Dephosphorylation of astrocyte elevated gene-1 protein upregulates eIF4E expression to promote gastric cancer progression.

Protein phosphorylation modification plays an important role in regulating protein activity. Astrocyte elevated gene-1 (AEG-1), an adaptor protein, promotes the progression of various types of cancers by protein-protein interactions. We previously demonstrated that AEG-1 promoted the growth and metastasis of gastric cancer by upregulating the expression of oncogenic eukaryotic translation initiation factor 4E (eIF4E).

Crystallinity-Directed In Situ Reconstruction of Cu-Al Oxides Yielding Tunable Cu Sites for Electrochemical CO-to-C Conversion.

During the electrochemical CO reduction reaction (CORR), copper catalysts continuously undergo structural evolution, which is less controllable, and its impact on the product distribution of CORR remains unclear. Here, crystallinity-tunable Cu-Al mixed metal oxide (CuAl-MMO-) precatalysts were first synthesized via layered double hydroxide calcination. These precatalysts subsequently underwent in situ electrochemical reconstruction to form active CuAl-MMO-R catalysts with tailored Cu valence states.

Selective Functionalization of Benzene Ring via Carbene-Initiated [4,5]-Rearrangement.

The extreme similarity in electronic and steric reactivity among the C─H bonds present on alkyl-substituted aromatic rings presents a formidable challenge when it comes to achieving high selectivity during the para-selective functionalization of these bonds. This article presents a unique study that delves into the para-selective functionalization or dearomatization functionalization of benzene rings, achieved through the reaction of carbenes with allyl sulfoxides. Mechanistic investigations suggest that the transformation likely proceeds via an uncommon carbene-mediated [4,5]-rearrangement process.

Analysis of Factors Influencing Cardiovascular Events and Mortality in Patients on Dialysis after Parathyroidectomy.

Background: Renal secondary hyperparathyroidism (SHPT) represents a prevalent complication among dialysis patients, significantly impacting long-term prognosis. Parathyroidectomy (PTX) serves as a clinically effective therapeutic option for patients diagnosed with refractory secondary hyperparathyroidism.

Objective: This study aims to assess the impact of PTX on cardiovascular events (CVEs) and all-cause mortality in dialysis patients, as well as to analyze the incidence and potential determinants of postoperative cardiovascular events and all-cause mortality.

Targeting inflammation with chimeric antigen receptor macrophages using a signal switch.

Chimeric antigen receptor (CAR) T-cell immunotherapy has shown great success in clinical cancer, bringing hope to apply CAR strategies to other clinical settings. Here we developed a CAR macrophage (CAR-M) that recognizes the major inflammatory molecule tumour necrosis factor (TNF) and activates an intracellular IL-4 signalling pathway, thereby programming engineered macrophages for an anti-inflammatory function. CAR-M therapy has exhibited efficacy in mouse models of both acute and chronic inflammatory diseases.

Diosmin attenuates UUO-induced renal ferroptosis and fibrosis by inhibiting the HIF-1α/FABP4 signaling axis.

Background And Purpose: Renal fibrosis is a major pathological feature of chronic kidney disease (CKD) that poses significant therapeutic challenges owing to its irreversible nature. In this study, we aimed to investigate the effects of diosmin, a polyphenolic flavonoid with anti-inflammatory, antioxidant, and antifibrotic properties, on renal fibrosis and to explore the underlying mechanisms.

Study Design And Methods: Mouse renal fibrosis model induced by UUO surgery and an HK-2 cell fibrosis model stimulated by TGF-β1 were established to evaluate the effects of diosmin treatment on cell injury, inflammatory response, and ferroptosis.

β-Hydroxybutyrate aggravates LPS-induced inflammatory response in bovine endometrial epithelial cells by activating the oxidative stress/NF-κB signaling pathway.

Ketosis, a metabolic disorder characterized by elevated levels of ketone bodies in the blood or urine, is known to impair the health and productivity of dairy cows, leading to substantial economic losses in the dairy industry. When ketosis occurs in dairy cows, the levels of β-hydroxybutyrate (BHBA), an abundant form of ketone bodies, in the blood increase significantly. Elevated BHBA levels have been shown to negatively impact reproductive performance and increase the incidence of periparturient diseases in dairy cows, including mastitis and endometritis.

Creation of dual-purpose soybean germplasm for grain and forage by CRISPR/Cas9-mediated targeting mutation of GmFT2a and GmFT5a.

Soybean [Glycine max (L.) Merr.] seeds are a rich source of high-quality protein and edible oil, and their foliage supports the rearing of the economically significant insect, 'Doudan'.

A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice.

The circadian clock regulates various physiological processes in mammals. The core circadian clock gene Bmal1 is crucial for maintaining the oscillations of the circadian clock system by controlling the rhythmic expression of numerous circadian clock-controlled genes. To explore the transcriptional changes associated with Bmal1 deletion in liver tissues, we collected liver tissues from global and liver-specific Bmal1 knockout mice, along with their respective control groups, at two circadian time points (CT2 and CT14) and used them for transcriptome sequencing analysis.

A hemodialysis patient unable to walk - brown tumor as the culprit: Case report and review of the literature.

Introduction: Brown tumors are benign lesions caused by hyperparathyroidism and characterized by increased osteoclast activity and mass effect, which can lead to paraplegia when the spine is involved. Secondary hyperparathyroidism is common in patients on long-term hemodialysis therapy.

Case Report: We report the case of a 48-year-old man on regular dialysis who presented with leg weakness as well as back pain and was diagnosed with secondary hyperparathyroidism and thoracic spine tumor.

NR1D1 activation alleviates inflammatory response through inhibition of IL-6 expression in bovine endometrial epithelial cells.

Endometritis, an inflammatory disease affecting dairy cattle, causes substantial economic losses in the dairy industry. Conventional treatment using uterine infusion of antibiotics often results in bacterial resistance and antibiotic residues in milk. Thus, identifying novel, effective therapeutic targets for endometritis in dairy cows is necessary.

Optimizing secure multimedia communication in embedded systems a parallel convolutional neural network approach to RIS and D2D resource allocation.

With the rapid development of Internet of Things (IoT) services, technologies that leverage multimedia computer communication for information sharing in embedded systems have become a research focus. To address the challenges of low spectral efficiency and poor network flexibility in multimedia computer communications, this paper proposes a resource allocation scheme based on parallel Convolutional Neural Network (CNN). The scheme optimizes the base station beamforming vector and the Reconfigurable Intelligent Surface (RIS) phase shifts to maximize the secure transmission rate for cellular users (CUs), while ensuring normal and secure communication for device-to-device (D2D) users.

Genome-scale CRISPR-Cas9 screen identifies as a therapeutic target for wild-type non-small cell lung cancer.

Epidermal growth factor receptor-targeted (-targeted) therapies show promise for non-small cell lung cancer (NSCLC), but they are ineffective in a third of patients who lack mutations. This underlines the need for personalized treatments for patients with wild-type NSCLC. A genome-wide CRISPR/Cas9 screen has identified the enzyme phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase (), which is vital in de novo purine biosynthesis and tumor development, as a potential drug target for wild-type NSCLC.

Clinical application of bronchoalveolar lavage fluid metagenomics next-generation sequencing in cancer patients with severe pneumonia.

Objective: Metagenomic next-generation sequencing (mNGS), as an emerging technique for pathogen detection, has been widely used in clinic. However, reports on the application of mNGS in cancer patients with severe pneumonia remain limited. This study aims to evaluate the diagnostic performance of bronchoalveolar lavage fluid (BALF) mNGS in cancer patients complicated with severe pneumonia.

Global, regional and national burden of CKD in children and adolescents from 1990 to 2019.

Background: Chronic kidney disease(CKD) is one of the most prevalent non-communicable health concerns in children and adolescents worldwide; however, data on its incidence, prevalence, disability-adjusted life years (DALYs) and trends in the population are limited. We aimed to assess the global, regional and national trends in CKD burden in children and adolescents.

Methods: In this trend analysis based on the 2019 Global Diseases, Injuries, and Risk Factors Study, CKD incidence, prevalence and DALYs rates per 100 000 population for children and adolescents were reported at the global, regional and national levels, as well as the average annual percentage change (AAPC).

Prioritized regional management for antibiotics and heavy metals in animal manure across China.

High levels of antibiotics and heavy metals in animal manure pose a potential threat to both the ecological environment and public health. A regional knowledge of their distribution and risk assessment across China remains unclear. A dataset containing 4082 records covering a total of forty-two antibiotics and eight heavy metals was established for animal manure across China.

A novel synthesized prodrug of gemcitabine based on oxygen-free radical sensitivity inhibited the growth of lung cancer cells.

In the present study, we introduced the H O -sensitive thiazolidinone moiety at the 4th amino group of gemcitabine (GEM) to synthesize a new target compound named GEM-ZZQ, and then we confirmed its chemical structure by nuclear magnetic resonance spectroscopy. We further confirmed that GEM-ZZQ had a good chemical stability in different pH solutions and that it could be activated by H O to release GEM. Pharmacodynamic studies revealed that the growth inhibition of human normal epithelial cells was weaker by GEM-ZZQ than by GEM treatment and that the inhibition of various lung cancer cell lines by GEM-ZZQ was similar to that of GEM.

Investigation Tracing the Origin of Tan Sheep Visceral Tissues through Mineral Elements.

The traceability of quality mineral fingerprints in the viscera of Tan sheep from northwest China was studied. Twenty-five mineral elements in the heart and liver samples of Tan sheep were determined using an inductively coupled plasma mass spectrometer (ICP-MS), and the characteristics of the mineral elements in the visceral tissues of the Tan sheep were further analyzed in combination with a principal component analysis (PCA), hierarchical cluster analysis (HCA), and linear discriminant analysis (LDA) to establish a discriminant model and verify it. The results show that 11 elements (Ba, Ca, Cu, Fe, K, P, Ni, Se, Sn, Te, and Zn) in the Tan sheep heart samples had significant differences among different regions ( < 0.

Anion Exchange Facilitates the In Situ Construction of Bi/BiO Interfaces for Enhanced Electrochemical CO -to-Formate Conversion Over a Wide Potential Window.

Electrochemical reduction of CO (CO RR) into value-added products is a promising strategy to reduce energy consumption and solve environmental issues. Formic acid/formate is one of the high-value, easy-to-collect, and economically viable products. Herein, the reconstructed Bi O CO nanosheets (BOC NSs) are synthesized by an in situ electrochemical anion exchange strategy from Bi O SO as a pre-catalyst.

Exosomes from tubular epithelial cells undergoing epithelial-to-mesenchymal transition promote renal fibrosis by M1 macrophage activation.

Kidney fibrosis is the common final pathway of chronic kidney disease (CKD), and it is distinguished by inflammation, mesenchymal transition with myofibroblast formation, and epithelial-to-mesenchymal transition (EMT). Macrophages are protuberant inflammatory cells in the kidney, and their roles are dependent on their phenotypes. However, it remains unclear whether tubular epithelial cells (TECs) undergoing EMT can influence the phenotypes of macrophages and the underlying mechanisms during the development of kidney fibrosis.

Effect of Biodentine on Odonto/Osteogenic Differentiation of Human Dental Pulp Stem Cells.

This study aims to compare the biological characteristics of human dental pulp stem cells (hDPSCs) isolated from different-aged populations and examine the effects of Biodentine on proliferation and odonto/osteogenic differentiation of hDPSCs isolated from the elderly in vitro. hDPSCs were isolated from three different-aged populations: group A (≤18 years old), group B (19−59 years old), and group C (≥60 years old). The adhesion, proliferation, odonto/osteogenesis, and senescence were compared.