Novel pathogenic MAN2B2 variants cause systemic lupus erythematosus and dysregulated glycosylation.

Genetic factors have been demonstrated to play essential roles in the pathogenesis of systemic lupus erythematosus (SLE). Identifying novel disease-causing genes of SLE helps to reveal its molecular mechanisms and new therapeutic targets. In this study, we identified biallelic loss-of-function variants of MAN2B2 gene in five unrelated SLE patients by whole exome sequencing.

Extracellular vesicles of Gram-negative and Gram-positive probiotics.

Extracellular vesicles derived from probiotics have received considerable attention for their pivotal role in bacterial‒host communication. These nanosized, bilayer-encapsulated vesicles carry diverse bioactive molecules, such as proteins, lipids, nucleic acids, and metabolites. Currently, ample evidence has emerged that probiotic extracellular vesicles may modulate several processes of host physiological hemostasis and offer therapeutic benefits.

Genetic Characterization of Wild Soybean Collected from Zhejiang Province in China.

: Wild soybean could grow in different soil types and under diverse climate conditions, which provides rich genetic resources in the locality. It is important to understand the genetic diversity as well as phenotypic variation for soybean breeding. The objective of this study was to analyze the genetic and phenotypic characteristics of 96 wild soybean accessions collected in Zhejiang Province, and to explore the potential advantage of germplasm resources for further application.

Unlocking ginsenosides' therapeutic power with polymer-based delivery systems: current applications and future perspectives.

Ginsenosides, as the main active ingredient of Panax plants, have been found to have extensive pharmacological activity and clinical therapeutic potential in recent years. However, its inherent physical and chemical properties such as poor solubility and low intestinal permeability result in low bioavailability, severely limiting its clinical application and translation. To address these challenges, polymeric carriers-valued for their excellent biocompatibility, structural tunability, and intelligent response functions-have been engineered to: (i) enhance solubilization polymer conjugation and amphiphilic micellar encapsulation; (ii) achieve passive (EPR-mediated) and active (ligand-directed) tumor targeting to minimize off-target distribution; and (iii) enable on-demand drug release through pH-, ROS-, temperature-, and enzyme-responsive designs.

The role of SUMOylation in epithelial-mesenchymal transition.

Tumor metastasis remains a leading cause of mortality in cancer patients. The epithelial-mesenchymal transition (EMT) is pivotal for the invasion and metastasis of cancer cells, facilitating the transformation from epithelial to mesenchymal phenotypes and thereby altering cellular morphology and function. SUMOylation, a critical post-translational modification, orchestrates a myriad of cellular physiological responses by binding to lysine residues on target substrates.

A small heat shock protein Fmp28 influences virulence by regulating Als3 expression via the cAMP-PKA signaling pathway in .

Small heat shock proteins are evolutionarily conserved molecular chaperones with diverse cellular roles. However, our understanding of their functions in pathogenic microorganisms remains limited. In this study, we identified and characterized Fmp28, a novel small heat shock protein that enhances virulence in , and explored its regulatory mechanism.

Hepatitis viruses hijack cellular metabolic pathways to drive replication and disease: Implications for novel therapeutics.

Hepatitis viruses are one of the most common viruses threatening humans all over the world, which can lead to viral hepatitis and even increase the risk of hepatocellular carcinoma. Numerous investigations have shown that hepatitis viruses reprogram the metabolisms of infected cells, including alterations in glucose, amino acid, and lipid metabolic pathways, resulting in changes of the life cycles of viruses, the status of host cells and the development of diseases. In this review, we summarized how hepatitis viruses rewire cells' metabolisms to promote virus replication and pathogenesis, which contributes to the exploitation of potential therapeutic paradigms.

Novel mutation T853fs associated with inherited macrothrombocytopenia.

-related inherited thrombocytopenia (-related IT) is a hereditary disorder involving ribosomopathy and platelet dysfunction. Affected patients exhibit significant bleeding tendencies. To date, five affected pedigrees have been reported, all harboring mutations within the "ATPase associated with diverse cellular activities" (AAA) domain.

Effects of effort-reward imbalance on emergency nurses' health: a mediating and moderating role of emotional exhaustion and work-family conflict.

Background: Effort-reward imbalance (ERI) is a prevalent issue in the healthcare sector, particularly in the high-intensity, high-risk, and high-stress environment of the emergency department. This results in emergency department nurses bearing heavier workloads, responsibilities, and time commitments compared to their counterparts in other departments. ERI poses significant risks to their physical, psychological, and sleep quality.

Insights into gut fungi: fungal succession, viral interactions and clinical applications.

As an essential part of the human gut microbiota, gut fungi are vital in human homeostasis, physio-pathological processes and intestinal functions. Some studies have confirmed the crucial roles of gut fungi in viral infection. And in an era that viral infection proliferate unchecked, the meticulous investigation into the interplay between gut fungi and human viruses holds immeasurable worth.

A retrospective study on unfavorable 28-day neurological outcomes of stage II TB meningitis.

Tuberculous meningitis (TBM) is often associated with adverse neurological outcomes, however, there is a lack of relevant research. The aim of this study is to explore the risk factors that affect the poor prognosis of 28-day neurological function in patients with stage II TBM. A retrospective analysis was conducted on the clinical data of patients with stage II TBM who visited our hospital from January 1st, 2018 to August 1st, 2019 based on the staging criteria of the Medical Research Council.

Elucidating the tumor microenvironment interactions in breast, cervical, and ovarian cancer through single-cell RNA sequencing.

This study aimed to identify the key cell types and their interactions in gynecological oncology of breast cancer, cervical cancer, and ovarian cancer. Single-cell RNA sequencing was performed on tumor samples of gynecological oncology from the GEO database. Cell types were identified using SingleR and cell composition was analyzed to understand the tumor microenvironment (TME).

[Clinical characteristics and genotypes of patients with Congenital fibrinogen disorders].

Objective: To explore the clinical features and genetic mutation sites of 28 patients with Congenital fibrinogen disorders (CFDs).

Methods: A total of 28 unrelated CFDs patients admitted to Wenzhou People's Hospital from June 2018 to April 2023 were enrolled into this research. A total of 2.

Postoperative continuous saline bladder irrigation reduces active urinary cancer cells: a prospective study in NMIBC.

Purpose: There is a lack of clinical evidence on whether further clinical strategies are needed after TURBT combined with immediate bladder instillation. This study intends to establish a reliable quantitative assay for active urinary cancer cells (AUCC) and to investigate the clinical efficacy of continuous saline bladder irrigation (CSBI) as a feasible option by analyzing the perioperative AUCC changes in TURBT.

Methods: An AUCC assay was developed and its reliability was verified by single-cell whole genome sequencing.

An SDS-NaOH-based method to isolate genome of recombinant adeno-associated virus vectors for physical titer measurement.

Recombinant adeno-associated viruses (rAAVs) vectors are promising for their safety and sustained expression of genetic payloads across various tissues. These vectors consist of a protein capsid enclosing a 4.7 kb single-stranded DNA genome.

Oral care medications for the prevention and treatment of ventilator-associated pneumonia in intensive care unit.

This study aims to ameliorate the management of VAP in clinical practice and deliver more precise care in the ICU. Study selection using the appropriate critical appraisal tools was undertaken by three authors. This review provides an overview of empirical antibiotics, chlorhexidine, and povidone-iodine, which are currently commonly used in critical care.

Disrupting the bacterial language: quorum quenching and its applications.

Quorum sensing (QS) is a bacterial communication method closely linked with population density and regulates biofilm formation and the secretion of virulence factors through the release, recognition, and prompt response to small molecule signals. At low cell density, each bacterium produces a low concentration of QS signals that diffuse or are actively transported into the external environment. The accumulated QS signals in the external environment reach a threshold concentration when the bacterial population attains a certain density, enabling effective recognition and interaction of bacterial QS signals with their receptors.

Determination and Pharmacokinetics of Acetylcorynoline in Mouse Plasma by UPLC-MS/MS.

Acetylcorynoline is an alkaloid isolated from the tubers of Cham. et Schltdl. It has anti-inflammatory properties with the potential to treat Parkinson's disease.

Triterpenoid saponins in tea plants: A spatial and metabolic analysis using UPLC-QTOFMS, molecular networking, and DESI-MSI.

Triterpenoid saponins, bioactive compounds with pharmaceutical relevance and functional food potential, are abundant in tea plants (Camellia sinensis), yet their structural diversity and tissue-specific distribution remain insufficiently explored. Using high-resolution mass spectrometry, Feature-based Molecular Networking, and imaging mass spectrometry (IMS), we profiled 52 tea saponins, including two novel trisaccharide saponins with unique glycosylation patterns. Aerial tissues, particularly buds and leaves, were enriched with cinnamoyl-decorated tetrasaccharide saponins, whereas roots predominantly accumulated di- and trisaccharide saponins, with significant cultivar-specific variation.

Somatic gain-of-function mutation in TLR7 causes early-onset systemic lupus erythematosus.

Objectives: We identified a case of early-onset systemic lupus erythematosus (SLE) characterised by acute immune thrombocytopenia, recurrent fever, pneumonia, myocardial damage, thyroid dysfunction, lymphadenopathy, hepatosplenomegaly, and intracranial calcification. Our objective was to investigate the genetic and molecular mechanisms underlying the disease.

Methods: Whole exome sequencing and targeted sequencing were performed and a somatic mutation in TLR7 was identified.

Prevalence and factors associated with renal insufficiency in patients with lung cancer: A cross-sectional study.

Objective: The aim of this study was to determine the prevalence of renal insufficiency in patients with lung cancer and factors associated with its development.

Materials And Methods: All patients at the First Affiliated Hospital of Guangzhou Medical University from January 1 to December 31, 2016 who had lung cancer were included in this study. Baseline characteristics, including age, sex, clinical features, estimated glomerular filtration rate (eGFR), echocardiographic findings, electrocardiogram results, and biochemical indicators were collected retrospectively.

Elucidating the causal associations and mechanisms between circulating immune cells and idiopathic pulmonary fibrosis: new insights from Mendelian randomization and transcriptomics.

Background: Growing evidence indicates an association between circulating immune cell phenotypes and idiopathic pulmonary fibrosis (IPF). Although studies have attempted to elucidate the causal relationship between the two, further clarification of the specific mechanisms and causal linkages is warranted.

Objective: We aimed to conduct a two-sample Mendelian randomization (MR) analysis with transcriptomics data analysis to elucidate the causal relationship between circulating immune cells and IPF and to explore potential biomarkers.

Effect of peripheral blood absolute monocyte count at admission on relapse-free survival in patients with idiopathic thrombotic thrombocytopenic purpura in remission.

Introduction: Peripheral monocytes have been reported to correlate with clinical outcomes in various types of malignancies. Previous reports have also shown that acute-phase thrombotic thrombocytopenic purpura (TTP) plasma could induce the activation of monocytes. However, the significance of peripheral blood absolute monocyte count (AMC) in idiopathic TTP remains an unanswered question.

Source-free domain transfer algorithm with reduced style sensitivity for medical image segmentation.

In unsupervised transfer learning for medical image segmentation, where existing algorithms face the challenge of error propagation due to inaccessible source domain data. In response to this scenario, source-free domain transfer algorithm with reduced style sensitivity (SFDT-RSS) is designed. SFDT-RSS initially pre-trains the source domain model by using the generalization strategy and subsequently adapts the pre-trained model to target domain without accessing source data.

Research progress on the role of bypass activation mechanisms in resistance to tyrosine kinase inhibitors in non-small cell lung cancer.

The clinical application of small molecule tyrosine kinase inhibitors (TKIs) has significantly improved the quality of life and prognosis of patients with non-small cell lung cancer (NSCLC) carrying driver genes. However, resistance to TKI treatment is inevitable. Bypass signal activation is one of the important reasons for TKI resistance.