Matrix metalloproteinase-3 promotes arteriovenous fistula failure by regulating FAK-AKT signaling.

Objective- Surgically created upper extremity arteriovenous fistulae (AVF) are the preferred vascular access for patients requiring dialysis. It is estimated, however, that 50% of AVF fail within one year due to aggressive neointimal hyperplasia, which significantly increases morbidity and mortality. Matrix metalloproteinase-3 (MMP-3), also known as stromelysin-1, is a member of the metalloproteinase family that plays a critical role in the pathogenesis of many human disorders by degrading extracellular matrix and regulating molecular signaling pathways.

An Open-Label, Single-Arm, Phase II Trial of Sintilimab Plus Anlotinib for Metastatic Non-Small Cell Lung Cancer After First-Line PD-(L)1 Inhibitor.

Background: Although immune checkpoint inhibitors (ICIs) have markedly improved first-line management of non-small cell lung cancer (NSCLC), many tumors eventually escape control after anti-PD-(L)1 therapy, leaving a clear therapeutic gap. Preclinical studies and preliminary clinical data suggest that coupling ICIs with anti-angiogenesis therapy can yield complementary antitumor effects. Consequently, we launched this investigation to evaluate the therapeutic benefit and tolerability of sintilimab, a PD-(L)1-blocking monoclonal antibody, together with the oral multi-target anti-angiogenesis agent anlotinib in metastatic NSCLC individuals experiencing progression after first-line PD-(L)1 inhibition.

Development and Validation of a Nomogram for Prognostic Prediction in Ovarian Cancer Based on Tumor Proliferation and Immune-Related Biomarkers in Chinese Patients.

Objective: To develop a nomogram prediction model for ovarian cancer prognosis using tumor proliferation and immune-related biomarkers.

Methods: Between January 2018 and December 2023, clinical data were collected from 140 patients diagnosed with epithelial ovarian cancer (EOC). These patients were randomly allocated into a training cohort consisting of 84 patients and a validation cohort comprising 56 patients, adhering to a 6:4 ratio.

Mild Parkinsonian Signs and Incidence of Motoric Cognitive Risk Syndrome in Older Adults.

Objective: We aimed to compare the incidence of motoric cognitive risk syndrome (MCR)-a pre-dementia syndrome that is identified by a combination of slowed gait speed and cognitive complaints-and previous mild parkinsonian signs (MPS) in older adults without Parkinson disease who do not have dementia.

Design: Prospective cohort study.

Setting And Participants: The study included community-dwelling older individuals (≥65 years) who did not have MCR at baseline.

A Dual-Payload Bispecific ADC Improved Potency and Efficacy over Single-Payload Bispecific ADCs.

All current FDA-approved antibody-drug conjugates (ADCs) are single-target and single-payload molecules that have limited efficacy in patients due to drug resistance. Therefore, our goal was to generate a novel ADC that was less susceptible to single points of resistance to reduce the likelihood of patient relapse. We developed a dual-targeting, dual-payload ADC by conjugating a bispecific EGFR x cMET antibody to two payloads (MMAF and SN38) that had separate mechanisms of action using a novel tri-functional linker.

A Review of Acupuncture for the Treatment of Dry Eye Syndrome: Mechanisms, Efficacy, and Clinical Implications.

Dry eye disease (DED) is a prevalent ocular condition characterized by discomfort and vision impairment in affected individuals. This review discusses various etiologies of DED, including autoimmune, drug-induced, and mechanical factors, each with distinct underlying causes. Acupuncture, a traditional therapeutic modality, has garnered increasing attention in recent years as a potential complementary treatment for DED.

Therapeutic Outcomes and Biomarker Potential of of Neoadjuvant Chemotherapy in Patients With Stage IIIC Versus Stage IV Epithelial Ovarian Cancer.

Purpose: Epithelial ovarian cancer (EOC) remains one of the most lethal gynecological malignancies. Although treatment options for newly diagnosed advanced EOC include primary debulking surgery (PDS) or neoadjuvant chemotherapy (NACT) followed by interval debulking surgery (IDS), the comparative effectiveness of these strategies remains uncertain across different disease stages.

Materials And Methods: We conducted a retrospective analysis of 297 patients with EOC whose initial treatment strategy was guided by predicted primary resectability using the Suidan model.

Portable X-ray fluorescence of zinc and selenium with nail clippings - visit 3 of the Mother and Infant Nutrition Investigation (MINI).

Introduction: Recent advances in portable X-ray fluorescence (XRF) have opened up the possibility of a rapid and cost-effective method for measuring elemental content in nail clippings. Two elements of particular interest for intake assessment through nail clipping measurement are zinc and selenium. The New Zealand-based Mother and Infant Nutrition Investigation (MINI) provides an ideal testing ground for the application of portable XRF in the measurement of zinc and selenium.

Altered mitochondrial DNA methylation in blood in individuals with mild cognitive impairment.

Background: Previous studies reported that altered mitochondrial methylation in Alzheimer's disease (AD), however, whether epigenetic modifications in mitochondrial genomes contribute to preclinical AD remains unclear. This study aimed to investigate mitochondrial methylation changes in individuals with cognitive decline.

Research Design And Methods: We examined whole mitochondrial genome methylation in 50 individuals with mild cognitive impairment (MCI) and 50 individuals without MCI, using bisulfite amplicon sequencing, assessing methylation at 366 Cytosine-guanine oligodeoxynucleotide (CpG) sites.

Novel pathogenic MAN2B2 variants cause systemic lupus erythematosus and dysregulated glycosylation.

Genetic factors have been demonstrated to play essential roles in the pathogenesis of systemic lupus erythematosus (SLE). Identifying novel disease-causing genes of SLE helps to reveal its molecular mechanisms and new therapeutic targets. In this study, we identified biallelic loss-of-function variants of MAN2B2 gene in five unrelated SLE patients by whole exome sequencing.

Precision Molecular Engineering of Alternating Donor-Acceptor Cycloparaphenylenes: Multidimensional Optoelectronic Response and Chirality Modulation via Polarization-Driven Charge Transfer.

In this study, three alternating donor-acceptor (D-A) type [12]cycloparaphenylene ([12]CPP) derivatives ([12]CPP 1a, 2a, and 3a) were designed through precise molecular engineering, and their multidimensional photophysical responses and chiroptical properties were systematically investigated. The effects of the alternating D-A architecture on electronic structure, excited-state dynamics, and optical behavior were elucidated through density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations. The results show that the alternating D-A design significantly reduced the HOMO-LUMO energy gap (e.

Physically enhanced neural network based on projection map synthesis for chemiluminescence tomography reconstruction with limited views.

In many combustion systems, data collection through optical windows is often hindered by fixed mechanical components that limit the number of available observation angles. This restriction poses a significant challenge to the accurate reconstruction of chemiluminescence tomography images with limited views. To address this limitation, we propose a novel projection interpolation approach for physically enhanced neural networks (PIPEN) to address this limitation.

Cerium oxide nanoparticles attenuates fibrosis and inflammation in thyroid-associated ophthalmopathy via JNK pathway.

Objective: Thyroid-associated ophthalmopathy (TAO) is an autoimmune orbital disorder characterized by pathological alterations including extraocular muscle fibrosis and orbital inflammation. Cerium oxide nanoparticles (CeO2-NPs, CNPs) are gaining popularity in ophthalmology due to their potential antifibrotic and anti-inflammatory properties. This study aims to investigate the inhibitory effects of CNPs on fibrosis and inflammation in TAO orbital fibroblasts (OFs) derived from TAO patients.

Investigation of Corrosion Resistance of 60Si2MnA Spring Steel Coated with Zn-Al in Atmospheric Environments.

To investigate the corrosion resistance of 60Si2MnA spring steel coated with Zn-Al in a domestic atmospheric environment containing harmful salts, the corrosion environmental factors (temperature, humidity, deposited salts, and pH) were obtained through field research. The deliquescence and weathering behavior of harmful salts were studied using impedance methods to establish their characteristic curves. Additionally, a self-designed salt deposition test apparatus was employed to conduct accelerated atmospheric corrosion tests under constant salt deposition (10 g/m) and controlled temperature and humidity conditions (20 °C/75% RH and 40 °C/75% RH) over different corrosion periods.

Optimizing Lidocaine Dosing in Hepatectomy Patients: A Population Pharmacokinetic Study of Active Metabolites.

Background: Lidocaine and its active metabolites are metabolized mainly by the liver, and liver-compromised may slow the metabolism of lidocaine and its active metabolites. In addition to excessive lidocaine, accumulated active metabolites may also lead to lidocaine-related toxicity in liver-compromised patients. This study aimed to describe the population pharmacokinetics of lidocaine and its active metabolites in partial hepatectomy patients and propose a novel drug regimen involving lidocaine-weighted active metabolites.

Functional nanomaterials for enhanced tumor photothermal therapy - the mechanisms and applications.

Photothermal therapy (PTT) offers revolutionary breakthroughs in tumor treatment due to its minimally invasive nature, high selectivity and efficiency. Photothermal therapy is a method of using laser irradiation (near-infrared light) to convert light energy into heat, reaching a relatively high temperature to kill tumor cells. Efficient and stable photothermal conversion materials are the key factors in PTT.

Single cell transcriptomic analysis reveals transcriptome differences of different cells between eosinophilic chronic rhinosinusitis with nasal polyps and non-eosinophilic chronic rhinosinusitis with nasal polyps.

Background: Chronic rhinosinusitis (CRS) with nasal polyps is a heterogeneous chronic inflammatory disease generally divided into two phenotypes including eosinophilic CRS with nasal polyps (eCRSwNP) and non-eosinophilic CRS with nasal polyps (neCRSwNP). However, its pathogenesis remains largely unknown. The aim of this study was to explore mechanistic differences between eCRSwNP and neCRSwNP using a bioinformatics approach.

Complete biosynthesis of salicylic acid from phenylalanine in plants.

Salicylic acid (SA) is a pivotal phytohormone for plant responses to biotic and abiotic stresses. Plants have evolved two pathways to produce SA: the isochorismate synthase and phenylalanine ammonia lyase (PAL) pathways. Whereas the isochorismate synthase pathway has been fully identified, the PAL pathway remains incomplete.

NFIC suppressed the epithelial ovarian cancer via modulating the balance of PTEN/TGFβ1/EGR1/BRD4 and SP1/EZH2 induced Inhibition of TBX2/MMPs signaling.

This study aims to explore the impact of NFIC and its regulated signaling factors on epithelial ovarian cancer, providing new insights for the treatment of ovarian epithelial cancer. Bioinformatics methods were applied to predict and analyze NFIC and its downstream signaling factors. In vivo experiments involved dividing 27 purchased female nude mice into three groups: NC group, NFIC-OE group, and NFIC-OE + TBX2-OE group, to observe tumor growth in each group.

[Two cases of Coffin-Siris syndrome type 3 caused by gene mutations].

Patient 1, a 3-year-6-month-old male, presented with feeding difficulties and delayed motor development. He exhibited poor responsiveness at birth, weak crying, intellectual and motor delays, low immunity, recurrent respiratory infections, hypotonia of the limbs, and distinctive facial features (low-set ears, double chin, and high arched palate), as well as a single transverse palmar crease on the right hand. Genetic testing revealed a c.

METTL3 alleviates renal tubular mitochondrial dysfunction by regulating the TUG1/PGC-1a axis in an IGF2BP2-dependent manner in diabetic nephropathy.

Purpose: To explore the N6-methyladenosine (mA) modification mechanism of taurine upregulated gene 1 (TUG1) and whether methyltransferase 3 (METTL3) can promote peroxisome proliferators-activated receptor γ coactivator 1 alpha (PGC-1α) transcription and alleviate mitochondrial dysfunction.

Methods: high glucose (HG)-treated HK-2 cell models and db/db mice models injected with rAAV-METTL3 the tail vein were established. The expression levels were determined by RT-qPCR, western blot, and immunohistochemical staining.

Analysis of astrocyte progenitors derived from human induced pluripotent stem cells and following transplantation into the intact spinal cord.

Functional improvement following traumatic spinal cord injury (SCI) remains limited, therefore, it is necessary to develop therapeutic interventions such as cell transplantation to replace lost cells and promote connectivity. While transplantation typically focuses on neurons, it is important to include other neural cells, such as immature astrocytes, to provide a permissive environment, promote neuroprotection and regeneration, and ultimately restore connectivity. In this study, we leveraged cellular engineering using human induced pluripotent stem cells (hiPSCs) to generate astrocyte progenitor cells (hAPCs).

Impact of blood lipid levels on breast cancer prognosis: a systematic review and meta-analysis.

Background: Breast cancer has emerged as the predominant malignant neoplasm globally, with potential implications for patient prognosis based on blood lipid profiles. This study aims to systematically review and meta-analyze the influence of lipid levels on the prognostic outcomes of individuals with breast cancer.

Methods: A thorough search was performed across multiple academic databases, including Embase, Cochrane, PubMed, Web of Science, CNKI, and Wanfang Database, up to March 2024.