O-GlcNAcylation of CEP44 Promotes Its Droplet Formation and Regulates Its Localization.

The centrosomal protein of 44 kDa (CEP44) is essential for centriole duplication, centrosome cohesion, and spindle integrity. It localizes to the proximal end of centrioles and associates with spindle microtubules. Liquid-liquid phase separation (LLPS) is a process by which biomolecules undergo demixing into distinct liquid-like phases, facilitating the formation of cellular condensates such as the centrosome.

ENKD1 Modulates Skin Elasticity Through Microtubule Stability Regulation.

Skin elasticity is critical for maintaining skin function, yet the molecular mechanisms governing this process remain incompletely understood. Herein, we identify enkurin domain-containing protein 1 (ENKD1) as a key regulator of skin elasticity by modulating microtubule stability in basal keratinocytes. In Enkd1 knockout mice, impaired migration of basal keratinocytes results in reduced epidermal elasticity compared to wild-type controls.

Therapeutic Outcomes and Biomarker Potential of of Neoadjuvant Chemotherapy in Patients With Stage IIIC Versus Stage IV Epithelial Ovarian Cancer.

Purpose: Epithelial ovarian cancer (EOC) remains one of the most lethal gynecological malignancies. Although treatment options for newly diagnosed advanced EOC include primary debulking surgery (PDS) or neoadjuvant chemotherapy (NACT) followed by interval debulking surgery (IDS), the comparative effectiveness of these strategies remains uncertain across different disease stages.

Materials And Methods: We conducted a retrospective analysis of 297 patients with EOC whose initial treatment strategy was guided by predicted primary resectability using the Suidan model.

Pediatric Firearm-Related Hospital Encounters by Child Opportunity Index Level.

Backround And Objectives: Whether variables specific to children's neighborhood-level social determinants of health are associated with neighborhood incidence of pediatric firearm-related injury hospital encounters has not been assessed in any entire state. This study sought to determine the relationship between home zip code Childhood Opportunity Index (COI) and zip code incidence of pediatric firearm-related injury hospital encounters in 4 geographically representative states.

Methods: This retrospective observational cohort study combined 2016-2021 discharge data from Florida, Maryland, New York, and Wisconsin with COI 3.

Diminished rest-activity rhythm is associated with postoperative complications and mortality: A prospective cohort study of UK Biobank participants.

Background: Circadian rhythm disruption, as a modifiable risk factor, has been increasingly recognised for its potential impact on adverse health outcomes, particularly in surgical populations where its implications warrant further investigation.

Objectives: To investigate associations between postoperative outcomes and preoperative rest-activity rhythm metrics from accelerometry.

Design: A cohort analysis of UK Biobank participants undergoing major surgery within 1 year of accelerometer monitoring.

Association of disrupted circadian rhythms with self-reported periodontal diseases: Insights from 94,305 UK biobank participants.

Background: This study investigates the association of circadian rhythmicity, physical activity, and chronotype with periodontal diseases, focusing on both the independent and combined effects.

Methods: A cross-sectional study was conducted among 94,305 participants from the UK Biobank. Circadian rhythmicity, measured by relative amplitude from the accelerometer, was the primary exposure, with physical activity and chronotype (morning/evening preference) as secondary exposures.

Outbreeding of isofemale lines as a candidate approach to improve the quality of thelytokous Wolbachia-infected line of the egg parasitoid, Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae.

The egg parasitoids, Trichogramma spp. are important biological control agents targeting various lepidopteran pests in agriculture. However, laboratory rearing often reduces genetic diversity, and potentially decreased fitness of Trichogramma wasps.

Risk factors for traumatic fracture related infection in surgically treated ankle fracture: A multicenter retrospective study.

Purpose: Infection after ankle fracture fixation is a serious complication, leading to a prolonged healing period and dysfunction in a long-term follow-up. Identifying risk factors for ankle fracture-related infection (FRI) is beneficial in preventing complications and reducing the risk of treatment. The study aims to retrospectively analyze clinical risk factors for ankle FRI.

Outcomes of surgically treated traumatic neuromas of cutaneous nerves as complications of ankle arthroscopy: A 5-year follow-up.

Objectives: To investigate the long term result of traumatic neuromas as complications of ankle arthroscopy treated by neuroma resection.

Methods: Twenty-five patients diagnosed with traumatic neuroma as complications of ankle arthroscopy were included. The standard procedure of microsurgical neuroma resection and nerve release was performed in all patients.

Construction and application of the "coordinate multidisciplinary care model" in elderly patients undergoing radical cystectomy based on QCC.

Objective: This study aims to develop and evaluate a multidisciplinary care model utilizing Quality Control Circle (QCC) methodology for elderly patients undergoing radical cystectomy.

Methods: This comparative study included patients who underwent radical cystectomy at Zhujiang Hospital between May 2022 and July 2023 (pre-improvement group, ​= ​65) and between August 2023 and July 2024 (post-improvement group, ​= ​41). The QCC team implemented four key interventions: a case manager-led collaborative team, a comprehensive care information platform, tiered holistic care management protocols, and an evidence-based referral system.

DDX5 super-enhancer promotes vasculogenic mimicry formation and metastasis in nasopharyngeal carcinoma by enhancing ADAM10 transcription.

Anti-angiogenic therapies (AATs) exhibit limited efficacy, as most patients with cancer inevitably develop resistance to them. In this study, data generated using a nasopharyngeal carcinoma orthotopic mouse model, combined with clinical data, reveal compensatory vasculogenic mimicry (VM) formation during AAT treatment and the association of VM with poor prognosis in nasopharyngeal carcinoma. Additionally, data-independent acquisition mass spectrometry-based proteomics shows that upregulation of a disintegrin And metalloprotease 10 (ADAM10) contributes to VM.

Improved VPS4B O-GlcNAc modification triggers lipid droplets transferring from adipocytes to nasopharyngeal carcinoma cells.

Background: The tumor microenvironment (TME) supplies critical metabolites that support cancer cell survival and progression. Adipocytes support tumor progression by secreting free fatty acids (FFAs) and adipokines; however, the role and mechanisms underlying lipid droplet (LD) release from adipocytes remain elusive.

Methods: Using two nasopharyngeal carcinoma (NPC) cell lines and primary human pre-adipocytes (HPA), we evaluate the effect of LDs on cell growth, proliferation, colony formation, and migration.

Study on the Microstructure and Mechanical Properties of 7085 Aluminum Alloy Reinforced by In Situ (ZrB + AlO) Nanoparticles and Rare Earth Er.

This study investigates the synergistic strengthening effects of in situ synthesized nano (ZrB + AlO) particles and rare earth Er microalloying on the microstructure and mechanical properties of 7085 aluminum alloy. The composite material was prepared through a melt direct reaction combined with rolling and T6 heat treatment, with microstructural evolution characterized by metallurgical microscopy, XRD, and SEM. Results demonstrate that the addition of 3 vol.

Association between maternal smoking during pregnancy and smoking behaviors in adult offspring.

Background: Maternal smoking during pregnancy (MSP) is associated with offspring smoking. However, there is still scant evidence to support the association between MSP and smoking behaviors in adult offspring.

Methods: This is a prospective cohort study based on the UK Biobank.

Coordination of IFT20 With Other IFT Components Is Required for Ciliogenesis.

Background: Primary cilia are organelles formed on the cell surface. They can act as cellular antennae to sense signals and play important roles in various biological processes. Abnormalities in primary cilia lead to a variety of diseases collectively known as ciliopathies.

Association of Oxygenation Targets and Mortality in Critical Care Patients Following Cardiac Surgery: A Retrospective Cohort Study.

Objective: To investigate the relationship between arterial oxygen partial pressure (PaO) and all-cause mortality of post-cardiac surgery patients.

Design: A retrospective cohort study based on the Medical Information Mart in Intensive Care-III (MIMIC-III) database.

Setting: The MIMIC-III database is a dataset comprising the clinical data of patients admitted to intensive care units (ICUs) at the Beth Israel Deaconess Medical Center.

A Mixed-Methods Evaluation of a Collaborative-Wide Quality Improvement Project to Improve Postdischarge Venous Thromboembolism Chemoprophylaxis After Abdominopelvic Cancer Surgery.

Objective: We studied a collaborative-wide quality improvement project (CQIP) focused on improving postdischarge venous thromboembolism (VTE) chemoprophylaxis adherence. We aimed to identify patient-level characteristics associated with adherence, evaluate differences in adherence rates among participating hospitals, and assess facilitators and barriers to adherence at high- and low-performing hospitals.

Background: VTE is the most common preventable cause of death after abdominopelvic cancer surgery, yet adherence to guideline-recommended postdischarge VTE chemoprophylaxis remains suboptimal.

Association between inequalities in mental health resources and burdens of mental health disorders in 146 countries and territories: An observational study.

Background: Mental health disorders impose a substantial social and economic burden. We conducted a comprehensive assessment of global mental health resource allocation and the associated burdens of mental health disorders, examining the relationship between these two factors.

Methods: We utilized data from the WHO Global Health Observatory and GBD 2021 to encompass 146 countries.

EFNA2 Mediates Stiffness-Regulated Hypopharyngeal Cancer Progression.

Objectives: Hypopharyngeal cancer (HPC), originating from the hypopharyngeal mucosa, is associated with a poor prognosis. Extracellular matrix (ECM) stiffness is closely linked to tumor progression and patient outcomes. This study aimed to investigate the effects of matrix stiffness and to identify molecular markers relevant to HPC prognosis, with the goal of improving clinical outcomes.

EZH2 inhibits senescence-associated inflammation and attenuates intervertebral disc degeneration by regulating the cGAS/STING pathway via H3K27me3.

Objective: Senescent nucleus pulposus mesenchymal stem cells (NPMSCs) are key instigators of local chronic inflammation and disruptions in nucleus pulposus tissue repair in intervertebral disc degeneration (IVDD). This study aimed to investigate the interplay between EZH2 and NPMSCs senescence-associated inflammation.

Methods: Nucleus pulposus samples were collected from IVDD patients (n = 15, F/M = 7/8, average age 47.

Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4.

Background: Porcine circoviruses 3 (PCV3) and 4 (PCV4) are emerging pathogens with global implications for swine industry, disturbing the diagnosis of PCVs associated diseases due to a range of similar clinical symptoms and increasingly coinfections. A rapid and accurate method for detection of PCV3 and PCV4 is critical for controlling the transmission of associated disease.

Methods: We developed a duplex real-time recombinase aided amplification (RAA) assay for detection of both PCV3 and PCV4 simultaneously.

TBC1D20 coordinates vesicle transport and actin remodeling to regulate ciliogenesis.

TBC1D20 deficiency causes Warburg Micro Syndrome in humans, characterized by multiple eye abnormalities, severe intellectual disability, and abnormal sexual development, but the molecular mechanisms remain unknown. Here, we identify TBC1D20 as a novel Rab11 GTPase-activating protein that coordinates vesicle transport and actin remodeling to regulate ciliogenesis. Depletion of TBC1D20 promotes Rab11 vesicle accumulation and actin deconstruction around the centrosome, facilitating the initiation of ciliogenesis even in cycling cells.

Raman Spectroscopy and Exosome-Based Machine Learning Predicts the Efficacy of Neoadjuvant Therapy for HER2-Positive Breast Cancer.

Early prediction of the neoadjuvant therapy efficacy for HER2-positive breast cancer is crucial for personalizing treatment and enhancing patient outcomes. Exosomes, which play a role in tumor development and treatment response, are emerging as potential biomarkers for cancer diagnosis and efficacy prediction. Despite their promise, current exosome detection and isolation methods are cumbersome and time-consuming and often yield limited purity and quantity.

Surgical correction of cavus foot may promote quality of life in patients with Charcot-Marie-Tooth disease: A retrospective study.

Charcot-Marie-Tooth disease (CMT) is a hereditary peripheral neuropathy leading to neuromuscular impairments, muscle atrophy, and functional limitations. Currently, no specific treatment exists to restore muscle strength in patients with CMT, and the disease can be severely disabling. Surgical correction of cavus foot has been suggested as a potential intervention to alleviate pain and improve gait in selected patients.

Development and validation of non-invasive prediction models for assessing kidney histopathological activity index in lupus nephritis.

Objective: To develop and validate prediction models for estimating the activity index (AI) of kidney histopathology in lupus nephritis (LN) using clinical and laboratory data.

Methods: This study used single-center data from patients with kidney biopsy-confirmed LN between January 2012 and March 2022. The development and validation datasets were temporally cut.