Multi-omics analysis revealing the taste characteristics and formation mechanism of Ocimum.

Ocimum, a plant of significant economic value, finds extensive applications in the food, spice, and pharmaceutical industries. This study integrated sensory evaluation, metabolomics, and transcriptomics to systematically analyze taste differentiation in four Ocimum accessions (G126, G081, G096, G124). Standardized quantitative descriptive analysis (QDA) revealed distinct taste profiles: G126 (O.

Gestational GenX Exposure Induces Maternal Hepatotoxicity by Disrupting the Lipid and Bile Acid Metabolism Distinguished from PFOA-Induced Pyroptosis.

Perfluorooctanoic acid (PFOA) and its replacement, GenX, are per- and polyfluoroalkyl substances (PFASs) widely used in industrial and consumer applications. Pregnant women are a vulnerable population to environmental pollutants. The maternal effects of GenX and PFOA exposure during pregnancy have not been fully elucidated.

Chlorogenic Acid Alleviates Early-Life GenX Exposure-Induced Neurotoxicity via Decreasing Lipopolysaccharide-Induced Pyroptosis by the Systemic Translocation and Suppressing the PI3K/AKT/NF-κB Pathway.

Ammonium perfluoro (2-methyl-3-oxahexanoate) (GenX), a substitute for perfluorooctanoic acid, disrupts early-life intestinal homeostasis and impacts neurodevelopment. However, the mechanisms are unclear, and interventions are limited. In this study, pregnant mice were exposed to GenX (2 mg/kg/day) and chlorogenic acid (CGA, 30 mg/kg/day) from gestation day 0 to postnatal day 21.

Lycopene ameliorates Di-(2-ethylhexyl) phthalate-induced neurotoxicity in mice via the gut-brain axis.

Background: Di(2-ethylhexyl) phthalate (DEHP), a ubiquitous plasticizer present in numerous consumer products, poses a substantial neurotoxic risk through environmental and dietary exposure. Growing evidence highlights a critical association between DEHP-induced neurotoxicity and gut microbiota dysbiosis. Renowned for its potent antioxidant and anti-inflammatory capabilities, the natural carotenoid lycopene (Lyc) demonstrates therapeutic promise in treating various neurological disorders.

Inulin alleviates intestinal changes caused by gestational PFOA and GenX exposure in the offspring of mice through reshaping maternal gut microbiota and vertically inhibiting the TLR4/NF-κB/NLRP3 inflammatory pathway in the pups.

Ammonium perfluoro (2-methyl-3-oxahexanoate) (GenX) is one of short-chain alternatives of perfluorooctanoic acid (PFOA) and has emerged as persistent global environmental pollutant due to its resistance to degradation. Nonetheless, the effects and mechanisms of perinatal exposure to GenX or PFOA on both mothers and offspring enterohepatic system remain poorly understood. In this study, pregnant C57BL/6J mice were administered 1 mg/kg body weight (bw)/day of PFOA or 2 mg/kg bw/day of GenX until the fourth week postpartum, with or without an inulin intervention at 5 g/kg bw/day.

Identification and validation of synergistic drug strategies targeting macrophage polarization in triple-negative breast cancer via single-cell transcriptomics and deep learning.

Triple-negative breast cancer (TNBC) presents therapeutic challenges due to its immunosuppressive tumor microenvironment (TME) and limited targeted options. Analyzing scRNA-seq data from 24 TNBC patients using integrated transcriptomics, machine-learning, and pseudo-time trajectory mapping, we developed a macrophage differentiation-based classifier (MMDCSS) (CD93, CHI3L1, ZBTB20) with remarkable prognostic accuracy (C-index: 0.929, 3-year AUC: 0.

Role of Tunneling Nanotubes in Arachidonic Acid Transfer and Macrophage Function Reprogramming in Intrahepatic Cholangiocarcinoma.

Tumor-associated macrophages (TAMs) play a crucial role in tumor progression within the tumor microenvironment (TME) through phenotypic plasticity and functional modulation. While tunneling nanotubes (TNTs) mediate intercellular communication, their role in shaping TAMs phenotypes and function remains unclear. This study explores how TNTs facilitate the transfer of tumor-derived materials, particularly fatty acids, to TAMs, affecting macrophage polarization and function.

Clinical prediction model by machine learning to determine the results of maternal dietary avoidance in food protein-induced allergic proctocolitis infants.

Objective: The objective of this study was to investigate the factors associated with the results of maternal dietary avoidance in infants diagnosed with Food Protein-Induced Allergic Proctocolitis (FPIAP). Additionally, we aimed to develop a predictive model using machine learning techniques to forecast the results of maternal dietary restrictions.

Methods: The clinical data of FPIAP infants were retrospectively analyzed.

The P53 Mutation Plays a Regulatory Role in Osteosarcoma Metastasis Via the Cholesterol-Hedgehog Pathway.

Background/aims: Osteosarcoma is the most common primary bone cancer affecting children and adolescents worldwide. Although many treatments for osteosarcoma have been explored, the overall survival rate for patients with metastatic osteosarcoma is only 20% due to the lack of understanding of the biological mechanisms by which osteosarcoma metastasis occurs. Therefore, it is important to uncover the molecular mechanism of metastasis in osteosarcoma.

Combining biomarkers to construct a novel predictive model for predicting preoperative lymph node metastasis in early gastric cancer.

Background: Accurately identifying the status of lymph node metastasis (LNM) is crucial for determining the appropriate treatment strategy for early gastric cancer (EGC) patients.

Methods: Univariate and multivariate logistic regression analyses were used to explore the association between clinicopathological factors and LNM in EGC patients, leading to the development of a nomogram. Differential expression analysis was conducted to identify biomarkers associated with LNM, and their expression was evaluated through immunohistochemistry.

MRI-based model to predict preoperative extrathyroidal extension in papillary thyroid carcinoma.

Objective: This study aimed to develop and validate a predictive model for preoperative extrathyroidal extension (ETE) in papillary thyroid carcinoma (PTC) using MRI features.

Methods: We retrospectively analyzed 140 confirmed PTC cases, divided into training (n = 84) and validation (n = 56) groups. MRI features such as T2-weighted imaging, multiphase contrast-enhanced MRI, and diffusion-weighted imaging were evaluated along with clinical data.

CXCL12 alone is enough to Reprogram Normal Fibroblasts into Cancer-Associated Fibroblasts.

Cancer-associated fibroblasts (CAFs) are critical components of the tumor microenvironment (TME), playing significant roles in regulating cancer progression. However, the underlying mechanism of CAFs activation remains elusive. In this study, we aim to investigates the mechanisms by which CAFs promote the conversion of normal fibroblasts (NFs) to CAFs in lung cancer, with a focus on the role of p53 mutations and the CXCL12/STAT3 signaling axis.

Multiomics analysis revealed the effects of polystyrene nanoplastics at different environmentally relevant concentrations on intestinal homeostasis.

Nanoplastics pollution is a global issue, with the digestive tract being one of the first affected organs, requiring further research on its impact on intestinal health. This study involved orally exposing mice to polystyrene nanoplastics (PS-NPs) at doses of 0.1, 0.

Expert consensus on intentional tooth replantation.

Intentional tooth replantation (ITR) is an advanced treatment modality and the procedure of last resort for preserving teeth with inaccessible endodontic or resorptive lesions. ITR is defined as the deliberate extraction of a tooth; evaluation of the root surface, endodontic manipulation, and repair; and placement of the tooth back into its original socket. Case reports, case series, cohort studies, and randomized controlled trials have demonstrated the efficacy of ITR in the retention of natural teeth that are untreatable or difficult to manage with root canal treatment or endodontic microsurgery.

Application of the Rosenthal effect combined with the CDIO teaching model to enhance core competencies in nursing interns.

Objective: This study aimed to investigate the impact of applying the CDIO teaching model, incorporating the Rosenthal effect, on the development of core competencies in nursing interns during clinical training.

Background: Possessing core competencies is of crucial importance for nursing students. Although new teaching methods have emerged, an effective model for the internship stage is still in demand.

Enhancing diagnostic accuracy of American College of Radiology TI-RADS 4 nodules: nomogram models based on MRI morphological features.

Background: Thyroid nodules classified as American College of Radiology Thyroid Imaging Reporting and Data System category 4 (ACR-TR4) present a diagnostic challenge due to their undetermined nature. This study aimed to develop and validate nomogram models using magnetic resonance imaging (MRI) morphological features to enhance the diagnostic accuracy of ACR-TR4 thyroid nodules, thereby reducing unnecessary fine-needle aspiration (FNA) and minimizing missed cancers.

Methods: We retrospectively analyzed 229 ACR-TR4 nodules from 184 patients who underwent preoperative MRI and surgical thyroidectomy between January 2017 and December 2022 in Minhang Hospital, Fudan University.

Single-Port Laparoscopic Choledochal Cyst Radical Surgery Using Mucosal Eversion Technique in Small-Diameter Hepaticojejunostomy.

Single-port laparoscopic choledochal cyst (CDC) excision and Roux-en-Y hepaticoenterostomy (SPCH) exhibits several advantages over conventional laparoscopic techniques in the management of CDCs. However, performing small-diameter hepaticojejunostomy during single-port laparoscopy presents significant challenges, thereby increasing the risk of anastomotic complications. In this study, we employed the mucosal eversion (ME) technique to alleviate the difficulties associated with executing small-diameter hepaticojejunostomy during SPCH and report the preliminary outcome of a prospective case cohort.

Gastrointestinal complications of hepatic glycogen storage disease: a national survey questionnaire study in China.

Background: Hepatic glycogen storage diseases (GSD) are inborn errors of metabolism with abnormal storage or utilization of glycogen, a complex disease with significant genetic heterogeneity and similar clinical manifestations. This study aimed to describe the gastrointestinal symptoms and endoscopic features of hepatic GSD, including types Ia, Ib, III, VI, and IX, to provide evidence for etiology and treatment.

Methods: A national cohort survey questionnaire was distributed to patients diagnosed with GSD type Ia, Ib, III, VI, and IX through genetic testing or their parents in mainland China in May 2022.

Expert consensus on pulpotomy in the management of mature permanent teeth with pulpitis.

Pulpotomy, which belongs to vital pulp therapy, has become a strategy for managing pulpitis in recent decades. This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing long-term patient-centered outcomes. Pulpotomy is categorized into partial pulpotomy (PP), the removal of a partial segment of the coronal pulp tissue, and full pulpotomy (FP), the removal of whole coronal pulp, which is followed by applying the biomaterials onto the remaining pulp tissue and ultimately restoring the tooth.

The role of the microbiota-gut-brain axis in methamphetamine-induced neurotoxicity: Disruption of microbial composition and short-chain fatty acid metabolism.

Methamphetamine (METH) abuse is associated with significant neurotoxicity, high addiction potential, and behavioral abnormalities. Recent studies have identified a connection between the gut microbiota and METH-induced neurotoxicity and behavioral disorders. However, the underlying causal mechanisms linking the gut microbiota to METH pathophysiology remain largely unexplored.

Identification and validation of serum MUC17 as a non-invasive early warning biomarker for screening of gastric intraepithelial neoplasia.

Background: The early diagnosis and treatment of Gastric Intraepithelial Neoplasia (GIN) are pivotal for improving the survival rates of patients with gastric cancer (GC). Regrettably, reliable noninvasive biomarkers for GIN screening are currently lacking.

Methods: mRNA data from the GEO database, pan-cancer data from the TCGA database, and a gene list of exocrine proteins were subjected to integrated analysis to identify a noninvasive biomarker for GIN.