The role of Na,K‑ATPase in lung diseases (Review).

Na,K‑ATPase, commonly referred to as the sodium pump, is known for its essential function as an ion pump, which is key for transporting Na and K across plasma cell membranes. Additionally, Na,K‑ATPase has multiple biological functions, independent of ion pumping, which are involved in regulating cell proliferation, apoptosis, differentiation and other processes associated with the development and progression of various diseases; particularly cancer, cardiovascular diseases, nervous system diseases, kidney diseases and lung diseases. As a potential therapeutic target, Na,K‑ATPase may represent a novel strategy for treating these diseases in the future.

Association between Ki-67 and clinical outcomes in 324 Chinese patients with resectable acral melanoma.

The relationship between Ki-67 expression and clinical prognosis in patients with resectable acral melanoma (AM) remains unclear. This study explores the prognostic role of Ki-67 in AM using data from 324 patients across five Chinese centers. Patients were divided into low Ki-67 expression and high Ki-67 expression groups based on pathological reports.

Deficiency of Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 in Pancreas Reduces β Cell Mass.

Although leucine-rich repeat-containing G protein-coupled receptor 4 (LGR4) is abundantly expressed in the pancreas, it is currently unknown whether LGR4 impacts pancreatic endocrine cells. Here, a critical role of LGR4 is demonstrated in islet β cell mass using a group of transgenic mice with LGR4 deficiency. Knock-out of Lgr4 in the pancreas and islet β cells significantly reduced islet β cell mass, and subsequently impaired glucose metabolism upon the challenge of a high-fat diet.

Maslinic acid alleviates ulcerative colitis by inhibiting the colitis-aggravating pathogen Clostridium perfringens and modulating gut microbiota.

Background: Ulcerative colitis (UC), a prevalent form of inflammatory bowel disease, continues to pose therapeutic challenges due to the limitations of conventional drugs and their inability to prevent relapse. Emerging evidence highlights the crucial role of gut microbiota dysbiosis in UC pathogenesis, yet our understanding of specific harmful microbes and their contributions to disease development remains limited.

Purpose: This study aims to (1) investigate the therapeutic potential of maslinic acid (MA), a food-derived natural compound, in colitis mice models; (2) elucidate the previously underestimated pathogenic role of Clostridium perfringens in UC development; and (3) reveal the molecular mechanisms underlying both C.

Simultaneous Decontamination for Ammonia Nitrogen and Phosphate Efficiently by Crystal Morphology MgO-Coated Functional Biochar Derived from Sludge and Sunflower Stalk.

Eutrophication driven by nitrogen and phosphorus discharge remains a critical global environmental challenge. This study developed a sustainable strategy for synergistic nutrient removal and recovery by fabricating MgO-coated biochar (Mg-MBC600) through co-pyrolysis of municipal sludge and sunflower stalk (300-700 °C). Systematic investigations revealed temperature-dependent adsorption performance, with optimal nutrient removal achieved at 600 °C pyrolysis.

Unraveling the Toxic Mechanisms of Microplastics in Aquatic Ecosystem: A Case Study on Vallisneria natans and Myriophyllum verticillatum.

Microplastics (MPs) have become ubiquitous pollutants in diverse ecosystems, with aquatic environments increasingly identified as major sinks. Nevertheless, the ecological risks and toxic effects of MPs on submerged macrophytes remain poorly understood. This study examined the impacts of three common MPs including polyvinyl chloride (PVC), polystyrene (PS) and polyethylene (PE) at varying concentrations (10mg/L; 50mg/L; 100mg/L) on two submerged plant, Vallisneria natans (V.

Structural and functional studies of the main replication protein NS1 of human parvovirus B19.

Parvovirus B19 (B19V) is a ubiquitous virus that can infect the majority of human population and cause erythema infectiosum, acute arthropathy, and many other diseases. The main replication protein NS1 plays a critical role in cell cycle arrest, transactivation of viral and host genes, and replication and package of B19V genome. Both DNA nicking and unwinding activities are required for the in vivo function of NS1, but the underlying basis is poorly understood.

Prevalence and Independent Risk Factors of Anxiety and Depression Symptoms in Glioma Patients: A Cross-Sectional Analysis.

Background: To investigate the current status of anxiety and depression symptoms in patients with brain glioma and identify the risk factors associated with anxiety and depression symptoms.

Methods: A total of 105 patients diagnosed with glioma at Longgang Central Hospital of Shenzhen from January 2021 to April 2024 were included in this study. The Hospital Anxiety and Depression Scale (HADS) was used to assess the anxiety and depression symptoms of the patients, who were then grouped based on their scores.

Intestinal Epithelial-Derived Exosomes Under Cold Stimulation Promote Adipose Thermogenesis.

: Whether intestinal epithelial cells can regulate distant adipose tissue remains a mystery. : Cold-stimulated intestinal epithelial cell-derived exosomes (Cold IEC-Exo) play a pivotal role in enhancing adipose thermogenesis and metabolic homeostasis, as demonstrated in this study. : IEC-Exo can accumulate in adipose tissue.

Isolation of Murine Pancreatic Stellate Cells and the Establishment of a New ex-vivo Activation Model.

Background: Pancreatic stellate cells (PSCs) are critical in the development of pancreatic fibrosis. In vitro, cell attachment itself can promote cell activation. Currently, there is a lack of methods for isolating activated PSCs that are unaffected by cell attachment.

Poor prognosis of early-stage acral melanoma with a history of trauma: a multicenter analysis of 468 patients.

Background: Previous studies have suggested that trauma may be a risk factor for the development and prognosis of acral melanoma (AM).

Methods: This population-based retrospective cohort study included patients with AM and a confirmed history of trauma who received treatment at 5 melanoma treatment centers in China. Factors associated with recurrence and survival were analyzed using univariate and multivariate Cox regression analyses.

Exploring methanogenic archaea and their thermal responses in the glacier-fed stream sediments of Rongbuk River basin, Mt. Everest.

Glacier-fed streams (GFS) are emergent sources of greenhouse gas methane, and methanogenic archaea in sediments contribute largely to stream methane emissions. However, little is known about the methanogenic communities in GFS sediments and their key environmental driving factors. This study analyzed stream sediments from the Rongbuk River basin on Mt.

Evaluation of PDZD11 in hepatocellular carcinoma: prognostic value and diagnostic potential in combination with AFP.

Background: Hepatocellular carcinoma (HCC) is the most prevalent liver cancer, with a 5-year survival rate below 20% and an average survival time of 3-6 months. Identifying new biomarkers is crucial for early diagnosis and prognosis. The function of PDZ domain protein 11 (PDZD11) in HCC remains unclear.

MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review.

Purpose: Acral melanoma (AM), a rare and aggressive melanoma subtype with poor prognosis, presents unique challenges in treatment due to its distinct molecular and immune characteristics. This case report describes a patient with AM harboring an AGK-BRAF fusion mutation, aiming to explore potential mechanisms of resistance to current treatment modalities.

Methods: We analyzed tumor tissue samples from the primary and metastatic lesions of the patient using next-generation sequencing (NGS) for genomic profiling and multiplex immunohistochemistry (mIHC) to assess the immune microenvironment.

Microbial Metabolic Limitations and Their Relationships with Sediment Organic Carbon Across Lake Salinity Gradient in Tibetan Plateau.

Inland lakes, contributing substantially to the global storage of sediment organic carbon (SOC), are subject to marked changes in salinity due to climate warming. The imbalance in the supply of resources, such as carbon, nitrogen, and phosphorus, in sediments leads to microbial metabolic limitations (MMLs). This, in turn, triggers the secretion of extracellular enzymes by microorganisms to mine for deficient resources by decomposing complex organic carbon.

Omadacycline successfully treated severe pneumonia after moxifloxacin treatment failure: Case series.

is a significant pathogen responsible for community-acquired pneumonia and, less commonly, for hospital-acquired pneumonia. , the most prevalent species within the genus, accounts for 80%-90% of human infections, and often leads to severe pneumonia complicated by multi-organ dysfunction. Omadacycline, a novel tetracycline, has demonstrated activity against atypical pathogens, including ; however evidence regarding its application in severe pneumonia remains limited.

Time-weighted kernel density for gearbox residual life prediction.

With improvements in industrial automation, the reliability of the gearbox, a key transmission device, has become increasingly crucial for the stable operation of an entire operating system. However, predicting the remaining useful life of the gearbox is challenging because of complex working environments and dynamic load changes. Several existing methods assume an inaccurate model structure and parameter estimation during life prediction, owing to the limited availability of similar fault sample data.

Deficiency of neuronal LGR4 increases energy expenditure and inhibits food intake via hypothalamic leptin signaling.

The metabolic effects of leucine-rich repeat-containing G protein-coupled receptor 4 (LGR4) remain largely unknown. Here, we showed that knockdown of Lgr4 in nestin progenitor or Sp1 mature neurons reduced high fat diet (HFD)-induced obesity by increasing energy expenditure and inhibiting food intake. Deficiency of LGR4 in AgRP neurons increased energy expenditure, and inhibited food intake, leading to alterations in glucose and lipid metabolism.

GPR180 reduces adiposity by inhibiting lipogenesis and fatty acid uptake in adipocytes.

In this study, we examined the effect of GPR180, a G protein-coupled receptor (GPCR) family member, on lipid metabolism of adipose tissue. We used adeno-associated virus overexpression of in subcutaneous adipose tissue, adipocyte-specific knockout mice and stromal vascular fraction (SVF) cells to explore the role and mechanism of GPR180 in lipid metabolism in adipocytes. Levels of mRNA in subcutaneous and epididymal adipose tissues were significantly reduced in mice fed high-fat diet (HFD).

Unravelling of the interaction mechanism of PFOA with submerged macrophytes and epiphytic biofilms at gene and molecular level.

Perfluorooctanoic acid (PFOA) is a global concern for the remediation of aquatic environments due to its toxicity, persistence and bioaccumulation. To gain a comprehensive understanding of the effects of PFOA on submerged macrophytes and epiphytic biofilms in eutrophic waters, as well as the mechanisms of PFOA and nutrient removal by submerged macrophyte-biofilm system, plant-attached biofilms were cultured under PFOA stress. Results indicated that PFOA exposure leads to an increase in ROS in plants, seriously damaging cellular systems, activating enzyme antioxidants to protect plant cells from oxidative damage.

Comparative study of the toxicity responses of Vallisneria natans and Pistia stratiotes to sulfadiazine under different planting methods.

Sulfonamides are receiving increased attention due to their persistence in the environment and potential ecological risks. However, there are currently relatively few studies on the toxicity response of aquatic plants grown under the single and mixed planting methods to sulfadiazine (SD). This study investigated the response of the Vallisneria natans (Lour.

Travertine Mineralogical Characteristics and Spatial Distribution in Huanglong, China, a World Natural Heritage: Derived from Late Pleistocene to Early Holocene Glacial U-Shaped Valley Carbonate Deposits.

In the long and complex process of geological evolution, the rise of the Himalayan movement and the strong Quaternary glacial movement 400,000 years ago have shaped the highly distinctive travertine landform landscape of Huanglong, China. The overflow of karst water in the high travertine layer has formed magnificent waterfalls and wonderful karst caves as well as the world's largest open-air travertine beaches and pools. The unique travertine landscape has entered the public's vision.

Waterborne Recoatable Transparent Superhydrophobic Coatings with Excellent Self-Cleaning and Anti-Dust Performance.

Superhydrophobic surfaces have attracted tremendous attention due to their intriguing lotus-leaf-like water-repelling phenomenon and wide applications, however, most superhydrophobic coatings are prepared with environmentally unfriendly organic solvents and suffer from poor mechanical strength. To solve these issues, waterborne recoatable superhydrophobic (WRSH) coatings are developed based on a novel self-synthesized water-soluble fluorinated acrylic polymer and hydrophobic modified silica nanoparticles. The trade-off between waterborne and superhydrophobicity is well mediated by protonation and deprotonation of the fluorinated acrylic polymer.