MiR-183-5p inhibitor promotes mitoxantrone-induced immunogenic death of hepatoma cells by targeting STC1.

This study aimed to unravel the regulatory mechanism of the microRNA (miR)-183-5p/STC1 axis in regulating mitoxantrone (MIT)-induced immunogenic cell death (ICD) within hepatocellular carcinoma (HCC) cells and assess its therapeutic potential. Dual-luciferase reporter assays were employed to validate that miR-183-5p directly interacts with the 3'-untranslated region (3'-UTR) of STC1 mRNA. HepG2 cells were subjected to treatment with MIT, either in the presence of miR-183-5p inhibitors or STC1 knockout.

Response of ornamental plants to salinity: impact on species-specific growth, visual quality, photosynthetic parameters, and ion uptake.

Ornamental horticulture provides substantial economic and environmental benefits, generating billions in annual sales and contributing to urban greening. However, the increasing scarcity of freshwater resources necessitates the use of alternative irrigation sources, such as reclaimed water. Reclaimed water typically contains elevated salt levels that can induce salt stress.

Decoding oxygen preference: Machine learning discovers functional genes in Bacteria.

Predicting bacterial oxygen preference and identifying associated genes is critical in microbiology. This study developed a machine learning model using genomic features to predict bacterial oxygen preference and discover potential functional genes. Trained on a dataset of 1813 bacterial genomes, a Random Forest model achieved 90.

Splenic volume as a predictor of survival in cancer patients treated with immune checkpoint inhibitors.

Objective: This investigation seeks to examine the association between spleen volume and prognosis in cancer patients undergoing immune checkpoint inhibitor (ICI) treatment.

Methods: We performed a retrospective analysis involving 61 patients diagnosed with hepatocellular carcinoma (HCC) who received ICIs at our institution. We evaluated the relationship between baseline splenic volume and its changes during ICI therapy concerning overall survival (OS) and progression-free survival (PFS) using a log-rank test.

Insight into soot oxidation performance and kinetics of novel Ce/La modified Cs-V based non-noble metal catalysts.

The catalytic diesel particulate filter (CDPF) is the most widely used after-treatment device for controlling diesel engine soot emissions. The development of cost-effective catalysts is crucial for diesel engines to comply with future ultra-low emission regulations. This paper studies a new type of Ce/La modified Cs-V non-noble metal CDPF catalyst.

Unveiling gut microbiota and metabolic functions contributed to polyvinyl chloride degradation in Spodoptera frugiperda larvae.

The accumulation of synthetic plastic waste, particularly polyvinyl chloride (PVC), threatens ecosystems globally. While microbial biodegradation represents a sustainable solution, limited effective PVC-degrading microbial bioresources have been identified. Here, we investigated the gut microbiota of Spodoptera frugiperda larvae, revealing a consistent microbial profile dominated by Enterococcus in both gut contents and tissues.

Self-Assembly of Short Peptides Activates Specific ER-Phagy and Induces Pyroptosis for Enhanced Tumor Immunotherapy.

Developing specific endoplasmic reticulum-autophagy (ER-phagy) inducers is highly desirable for discovering new ER-phagy receptors and elucidating the detailed ER-phagy mechanism and potential cancer immunotherapy. However, most of the current ER-phagy-inducing methods cause nonselective autophagy of other organelles. In this work, we report the design and synthesis of simple and stable short peptides (D-FFxFFs) that could specifically trigger ER-phagy, which further induces pyroptosis and activates the immune response against tumor cells.

GPR180 reduces adiposity by inhibiting lipogenesis and fatty acid uptake in adipocytes.

In this study, we examined the effect of GPR180, a G protein-coupled receptor (GPCR) family member, on lipid metabolism of adipose tissue. We used adeno-associated virus overexpression of in subcutaneous adipose tissue, adipocyte-specific knockout mice and stromal vascular fraction (SVF) cells to explore the role and mechanism of GPR180 in lipid metabolism in adipocytes. Levels of mRNA in subcutaneous and epididymal adipose tissues were significantly reduced in mice fed high-fat diet (HFD).

Mobile Resistance Elements: Symbionts That Modify Insect Host Resistance.

Mounting evidence indicates that symbionts play a beneficial role through secondary metabolic compounds and various chemical processes in host adaptation to adversity, particularly in herbivorous insects whose survival is severely threatened by insecticides or secondary metabolite stress. Despite extensive research on insect symbionts, the spread of these beneficial symbionts and the correlation with host phenotypes limit our ability to predict and manage the adaptive capabilities of insect populations in changing environments. In this review, we propose the concept of "Mobile Resistance Elements (MRE)" to describe the dynamic and adaptable nature of resistance-related symbionts that can be transmitted between insect hosts.

Symbiont community assembly shaped by insecticide exposure and feedback on insecticide resistance of Spodoptera frugiperda.

Exploring the mechanism of microbiota assembly and its ecological consequences is crucial for connecting microbiome variation to ecosystem function. However, the influencing factors underlying microbiota assembly in the host-microbe system and their impact on the host phenotype remain unclear. Through investigating the prevalent and worsening ecological phenomenon of insecticide resistance in global agriculture, we found that insecticide exposure significantly changed the gut microbiota assembly patterns of a major agricultural invasive insect pest, Spodoptera frugiperda.

Exploring microbial diversity and function in companion planting systems of white clover and orchard grass.

Companion planting of white clover (Trifolium repens L.) with orchard grass (Dactylis glomerata L.), a famous hay grass, improves the forage quality of orchard grass.

Effect of alternative fuels on the emissions of a passenger car under real-world driving conditions: a comparison of biodiesel, gas-to-liquid, coal to liquid.

Fossil fuel energy crisis and environmental pollution have initiated the scientific research on alternative fuels. Biodiesel (B100), gas to liquid (G100), and coal to liquid (C100) are superb selections to be substitutes for conventional diesel. To better investigate the emission characteristics of the alternative fuels mentioned above, a portable emission measurement system (PEMS) was used to carry out this study under real-world driving conditions.

Uninheritable but Widespread Bacterial Symbiont Mediates Detoxification of the Insecticide Chlorantraniliprole in the Agricultural Invasive Pest .

Host-symbiont interaction plays a crucial role in determining the host's fitness under toxic stress, as observed in numerous insect species. However, the mechanism of the symbionts involved in the detoxification of insecticides remains poorly known. In this study, through microbiome, proteomic, and genomic analysis, we identified a prevalent symbiont, EMBL-3, in a major invasive insect pest,.

Investigation of emission characteristics of a marine cargo ship in real-world conditions.

Pollution caused by ship emissions will considerably impact coastal areas. A test system that matched the actual conditions of a ship was designed based on a portable emission measurement system (PEMS), and the emission characteristic of gaseous and particle emissions and the particle size distribution of the ship's main engine were investigated under real-world operating conditions. The results showed that the emission concentrations of the main pollutants fluctuated greatly under the departure, anchoring, and docking conditions, and the peaks of CO, CO, and NO emissions appeared under these transient conditions.

The conformational modification of myofibrillar protein by magnetic field improves its emulsification properties.

This study investigated the effect of different magnetic field treatments (0, 3, 6, 9, 12 mT) on the structure and emulsification properties of myofibrillar protein (MP). The results showed that the emulsion stabilized by MP with 3, 6, 9 mT magnetic field treatments possessed higher emulsifying ability, storage stability and apparent viscosity, since magnetic field induced the structural unfolding of MP and exposed the hydrophobic groups (the surface hydrophobic increased from 30.10 to 43.

Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice.

Whether intestinal Leucine-rich repeat containing G-protein-coupled receptor 4 (LGR4) impacts nutrition absorption and energy homeostasis remains unknown. Here, we report that deficiency of Lgr4 (Lgr4) in intestinal epithelium decreased the proportion of enterocytes selective for long-chain fatty acid absorption, leading to reduction in lipid absorption and subsequent improvement in lipid and glucose metabolism. Single-cell RNA sequencing demonstrates the heterogeneity of absorptive enterocytes, with a decrease in enterocytes selective for long-chain fatty acid-absorption and an increase in enterocytes selective for carbohydrate absorption in Lgr4 mice.

Intermittent fasting promotes type 3 innate lymphoid cells secreting IL-22 contributing to the beigeing of white adipose tissue.

Mechanism underlying the metabolic benefit of intermittent fasting remains largely unknown. Here, we reported that intermittent fasting promoted interleukin-22 (IL-22) production by type 3 innate lymphoid cells (ILC3s) and subsequent beigeing of subcutaneous white adipose tissue. Adoptive transfer of intestinal ILC3s increased beigeing of white adipose tissue in diet-induced-obese mice.

Insight into the mechanisms of combining direct current magnetic field with phosphate in promoting emulsifying properties of myofibrillar protein.

This study investigated the combined effects of direct-current magnetic field (DC-MF, 9.5 mT) and tetrasodium-pyrophosphate (TSPP, 1-5 g/L) on emulsified gel properties of porcine myofibrillar protein (MP). Results showed that MP at DC-MF and 3 g/L TSPP had decreased spectrum intensity of UV and fluorescence compared to that without DC-MF, owing to the changes of MP tertiary structure caused by DC-MF, especially tryptophan and tyrosine.

promotes the proliferation of lactic acid bacteria and influences the fermentation quality of whole-plant corn silage.

Objective: This study aimed to investigate the promoting effect of a (BV) strain on lactic acid bacteria (LAB) and determine its influence on the fermentation quality and aerobic stability of silage.

Methods: Flat colony counting method was used to evaluate the effect of BV on the growth of LAB. Freshly harvested whole-plant corn was inoculated separately with BV and L.

Effect of magnetic field mediated CaCl on the edible quality of low-sodium minced pork gels.

Magnetic field combined with calcium chloride (CaCl,) treatment is a highly promising technique for reducing sodium chloride (NaCl) in meat. Therefore, this paper investigated the effect of reducing NaCl addition (0-10%) by CaCl in combination with a magnetic field (3.8 mT) on the edible quality of low-salt pork mince.

Polyp segmentation with interference filtering and dynamic uncertainty mining.

Accurate polyp segmentation from colo-noscopy images plays a crucial role in the early diagnosis and treatment of colorectal cancer. However, existing polyp segmentation methods are inevitably affected by various image noises, such as reflections, motion blur, and feces, which significantly affect the performance and generalization of the model. In addition, coupled with ambiguous boundaries between polyps and surrounding tissue, i.

A terbium(III) complex-based time-resolved luminescent probe for selenocysteine as an inhibitor of selenoproteins.

A terbium(III) complex-based time-resolved luminescence probe for selenocysteine can inhibit selenoprotein activity a selenolate-triggered cleavage reaction of sulfonamide bonds in living cells.

The performance of plant essential oils against lactic acid bacteria and adverse microorganisms in silage production.

Plant essential oils have played an important role in the field of antibiotic alternatives because of their efficient bacteriostatic and fungistatic activity. As plant essential oils are widely used, their activity to improve the quality of plant silage has also been explored. This review expounds on the active ingredients of essential oils, their bacteriostatic and fungistatic activity, and mechanisms, as well as discusses the application of plant essential oils in plant silage fermentation, to provide a reference for the development and application of plant essential oils as silage additives in plant silage fermentation feed.

Effect of catalyst diesel particulate filter aging and catalyst loadings on particulate emission characteristics from a diesel vehicle.

In this study, the effect of new and used catalyzed diesel particulate filter (CDPF) with different catalyst loadings on the particulate emissions including the particle mass (PM), particle number (PN), particle size distribution (PSD) and geometric mean diameter (GMD) from a diesel vehicle were investigated based on a heavy chassis dynamometer. Results showed that more than 97.9% of the PN and 95.

Effect and mechanism of DC magnetic field combined with calcium chloride on saltiness and flavor enhancement of low salt pork gel.

This study intended to investigate the synergistic effect of direct current magnetic field (DC-MF) and calcium chloride (CaCl) on improving the saltiness and quality of pork gel and to explore the potential adjustment mechanisms involved. Pork mince was subjected to low-salt treatment of DC-MF for 3 h (T1), CaCl substitution (T2) or DC-MF combined with CaCl (T3) respectively under high and low salt controls (HC and LC). Heat-induced gels of pork mince were prepared and analyzed in terms of gel texture, saltiness perception, moisture status, salt release and protein structure.