Genomic characterization of Gillisia xinjiangensis sp. nov.: insights into nitrogen metabolism, stress adaptation, and application potential.

High-altitude saline lakes host distinctive microbial communities characterized by specialized adaptations to extreme environmental conditions. However, the underlying mechanisms enabling bacterial survival and physiological function in these habitats remain largely unexplored. In this study, a novel Gram-stain-negative, non-motile, rod-shaped bacterium, designated strain Q332, was isolated from sediment samples collected from Achikkul Lake, a high-altitude saline lake in Xinjiang, northwestern China.

Temporal Trends and Regional Disparities in Rotavirus Vaccine Coverage for Children Under 1-Year-old in Shandong Province, China.

Background: Rotavirus vaccine is the most effective measure for preventing rotavirus-related diarrhea. The study aims to identify the coverage and trend of the rotavirus vaccine, providing insights for improving vaccination programs.

Methods: Rotavirus vaccination data from 2005 to 2024 were collected and analyzed using Joinpoint regression to identify trends and significant changes over time.

RORα-activated mitophagy attenuating hypoxic-ischemic encephalopathy via suppression of microglial cGAS-STING axis.

Introduction: Hypoxic-ischemic encephalopathy (HIE) involves neuroinflammation driven by microglial activation, yet regulatory mechanisms remain poorly defined. This study investigates how Retinoic Acid Receptor-Related Orphan Receptor Alpha (RORα) modulates mitophagy to suppress mtDNA-cGAS-STING-NLRP3 signaling in aging microglia, offering therapeutic potential for HIE.

Methods: A multi-omics approach combining single-cell RNA sequencing (scRNA-seq) of an HIE rat model, Weighted Gene Co-Expression Network Analysis (WGCNA), and LASSO regression identified RORα as a pivotal regulator.

Efficacy of carrimycin against complex and .

complex (MAC) is one of the principal pathogenic strains among nontuberculous . Current antibiotics have poor microbiological responses, and repurposed antibiotics are a way to meet clinical needs. Herein, the anti-MAC activity of carrimycin (CAM), a new macrolide, was evaluated vitro and .

sp. nov., a halophilic bacterium from a high-altitude saline lake exhibiting diverse metabolic potential and ecological adaptation.

Saline lakes are extreme habitats that host unique microbial communities with high biotechnological potential. In this study, a novel strain, designated Q87, was isolated from Gaxiukule Lake, a high-altitude magnesium sulfate-type saline lake in the Qaidam Basin, China. A polyphasic taxonomic approach, including morphological, physiological, chemotaxonomic, phylogenetic, and genomic analyses, was applied to characterize the isolate.

In vitro and in vivo antibacterial activity of sudapyridine (WX-081) combined with other drugs against Mycobacterium abscessus.

The management of Mycobacterium abscessus (MAB) infection presents formidable challenges, necessitating the development of innovative antimicrobial agents and more efficacious combination therapies. The objective of this study was to investigate the in vitro, intracellular, and in vivo antibacterial activity of sudapyridine (WX-081) in combination with clarithromycin (CLR), clofazimine (CFZ), rifabutin (RFB), and linezolid (LZD) against MAB. In vitro, the interaction between WX-081 and other drugs was assessed using the checkerboard method against MAB.

In vitro and in vivo activities of a novel benzothiopyranone candidate NTB-3119M against Mycobacterium tuberculosis.

Objectives: NTB-3119M, a novel benzothiopyranone derivative identified through comprehensive drug development studies, was selected as a promising antituberculosis (anti-TB) candidate. This study systematically evaluated its anti-TB efficacy in vitro and in vivo.

Methods: In vitro analyses encompassed antimicrobial susceptibility testing to determine minimum inhibitory concentrations (MICs) against Mycobacterium tuberculosis H37Rv, 10 drug-susceptible clinical isolates, and 30 multidrug-resistant (MDR) strains, alongside evaluations of minimal bactericidal concentrations (MBCs) using H37Rv and seven clinical isolates.

Taxonomic, genomic, and ecological insights into a novel Flavobacteriaceae strain from coastal tidal flats.

Background: Tidal flats are vital coastal ecosystems that play a significant role in organic carbon accumulation and biogeochemical cycles. Members of the family Flavobacteriaceae is known for its ability to degrade complex organic matter, including polysaccharides. However, the ecological roles and metabolic capabilities of Flavobacteriaceae in tidal flat environments remain underexplored.

Investigation of the enzymatic characteristics of thermotolerant xylanase McXyn0243 derived from Malbranchea cinnamomea, and its application in the degradation of three agricultural residues for the production of xylooligosaccharides.

Malbranchea cinnamomea produces enzymes essential for degrading lignocellulosic materials, converting agricultural waste into high-value compounds like xylooligosaccharides (XOSs). In this study, we cloned and expressed an endo-1,4-β-xylanase, McXyn0243, from M. cinnamomea and investigated its enzymatic properties and application in agricultural wastes degradation.

A narrative review on the effectiveness of PPSV23 vaccination in adults in China.

Pneumococcal Polysaccharide Vaccine (PPSV23) is available for free in some Chinese cities for elderly patients and those with comorbidities. However, there is a lack of studies summarizing its preventive effect specifically in Chinese adults. This review aims to discuss the epidemiology of pneumococcal disease, coverage and effectiveness of PPSV23 vaccination, elderly individuals and patients with comorbidities, coadministration of PPSV23 vaccine with other vaccines, and future directions for its use in China.

Decision Tree Extraction for Clinical Decision Support System With If-Else Pseudocode and PlanSelect Strategy.

Decision trees, as a structured representation of medical knowledge, are critical resources for building clinical decision support systems. Their structured decision pathways can be used for retrieval to enhance clinical decision making. Currently, mainstream methods mainly utilize large language models and in-context learning for decision tree extraction.

sp. nov. and sp. nov., two bacteria isolated from marine sediment in the East China Sea.

Two Gram-stain-negative, curved-rod-shaped, non-motile and aerobic bacteria W6 and I13 were isolated from marine sediment samples collected from Meishan Island located in the East China Sea. Catalase and oxidase activities and hydrolysis of Tween 40, 60 and 80 were positive for both strains, while nitrate reduction, indole production, methyl red reaction and HS production were negative. Phylogenetic analyses based on 16S rRNA and genome sequences revealed that strains W6 and I13 formed distinct phylogenetic lineages within the genera and , respectively.

Wip1 phosphatase activator QGC-8-52 specifically sensitizes p53-negative cancer cells to chemotherapy while protecting normal cells.

PP2C serine-threonine phosphatase Wip1 plays an important role in normal tissue homeostasis, stress signaling and pathogenesis of various human diseases. It is an attractive drug target for cancer treatment and inhibition of its expression or activity constitute a novel therapeutic intervention strategy to prevent the development of various cancers. However, previous strategies for Wip1 suppression may be ineffective in cancers lacking p53.

Function Switch of a Fungal Sesterterpene Synthase through Molecular Dynamics Simulation Assisted Alteration of an Aromatic Residue Cluster in the Active Pocket of PfNS.

Terpene synthases (TPSs) play pivotal roles in generating diverse terpenoids through complex cyclization pathways. Protein engineering of TPSs offers a crucial approach to expanding terpene diversity. However, significant potential remains untapped due to limited understanding of the structure-function relationships of TPSs.

sp. nov., a novel member isolated from coastal mudflat sediment of Meishan Islandin the East China Sea.

A Gram-negative, rod-shaped, non-motile and strictly aerobic strain, designated NBU2979, was isolated from a coastal mudflat located on Meishan Island in the East China Sea. Strain NBU2979 grew optimally at 32 °C, with 2.0 % NaCl (w/v) and at pH 7.

Widely targeted metabolomic and KEGG analyses of natural deep eutectic solvent-based saponins extraction from Camellia oleifera Abel.: Effects on composition.

Camellia saponins are important by-products of Camellia Oleifer Abel. processing. In this study, an eco-friendly method based on natural deep eutectic solvents (NaDESs, proline and glycerol at a molar ratio of 2:5) was established to extract saponins from C.

Birinapant Reshapes the Tumor Immunopeptidome and Enhances Antigen Presentation.

Birinapant, an antagonist of the inhibitor of apoptosis proteins, upregulates MHCs in tumor cells and displays a better tumoricidal effect when used in combination with immune checkpoint inhibitors, indicating that Birinapant may affect the antigen presentation pathway; however, the mechanism remains elusive. Based on high-resolution mass spectrometry and in vitro and in vivo models, we adopted integrated genomics, proteomics, and immunopeptidomics strategies to study the mechanism underlying the regulation of tumor immunity by Birinapant from the perspective of antigen presentation. Firstly, in HT29 and MCF7 cells, Birinapant increased the number and abundance of immunopeptides and source proteins.

Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket.

Fungal bifunctional terpene synthases (BFTSs) catalyze the formation of numerous di-/sester-/tri-terpenes skeletons. However, the mechanism in controlling the cyclization pattern of terpene scaffolds is rarely deciphered for further application of tuning the catalytic promiscuity of terpene synthases for expanding the chemical space. In this study, we expanded the catalytic promiscuity of fusoxypene synthase (FoFS) by a single mutation at L89, leading to the production of three new sesterterpenes.

Sudapyridine (WX-081) antibacterial activity against , , and and .

Unlabelled: Sudapyridine (WX-081) is a structural analog of bedaquiline (BDQ), which shows anti-tuberculosis and non-tuberculous mycobacteria (NTM) activities but, unlike BDQ, did not prolong QT interval in animal model studies. This study evaluated the antibacterial activity of this novel compound against , , and and . The minimum inhibitory concentration (MIC) of WX-081 against three kinds of non-tuberculous mycobacteria (NTM) clinical strains was determined using microplate-based alamarBlue assay (MABA), and the antibacterial activity of WX-081 against NTM in J774A.

Integration of multiple terminology bases: a multi-view alignment method using the hierarchical structure.

Motivation: In the medical field, multiple terminology bases coexist across different institutions and contexts, often resulting in the presence of redundant terms. The identification of overlapping terms among these bases holds significant potential for harmonizing multiple standards and establishing unified framework, which enhances user access to comprehensive and well-structured medical information. However, the majority of terminology bases exhibit differences not only in semantic aspects but also in the hierarchy of their classification systems.

An LF-NMR homogeneous immunoassay for Vibrio parahaemolyticus based on superparamagnetic 2D nanomaterials.

In this work, a sensitive low field nuclear magnetic resonance (LF-NMR) homogeneous immunoassay, also called magnetic resonance switch (MRSw) sensor, for Vibrio parahaemolyticus (VP) was developed. Superparamagnetic 2D nanomaterial was designed and used as the magnetic probe of MRSw sensor. It was GO@SPIONs&Ab, a composite nanomaterial with many superparamagnetic FeO nanoparticles (SPIONs) providing a magnetic signal and VP antibody (Ab) specifically recognizing the target VP evenly distributed on the surface of GO.