Conformational dynamics underlying slow inactivation in voltage-gated sodium channels.

Voltage-gated sodium (Nav) channels initiate and propagate action potentials in many excitable cells. Upon repetitive activation, the fraction of Nav channels available for excitation gradually decreases on a timescale ranging from seconds to minutes, a phenomenon known as slow inactivation. This process is crucial for regulating cellular excitability and firing patterns.

YOLOv5s-TC: An Improved Intelligent Model for Insulator Fault Detection Based on YOLOv5s.

Insulators play a pivotal role in power grid infrastructure, offering indispensable electrical insulation and mechanical support. Precise and efficient detection of insulator faults is of paramount importance for safeguarding grid reliability and ensuring operational safety. With the rapid advancements in UAV (unmanned aerial vehicle) technology and deep learning, there has been a notable transition from traditional manual inspections to automated UAV-based detection systems.

Correction: Han et al. Lotus Bee Pollen Extract Inhibits Isoproterenol-Induced Hypertrophy via JAK2/STAT3 Signaling Pathway in Rat H9c2 Cells. 2023, , 88.

In the original publication [...

Bee venom alleviates isoproterenol-induced cardiac hypertrophy via JAK2/NF-κB signaling cascade.

Pathological cardiac hypertrophy (CH) often progresses towards heart failure, which presents a serious threat to global public health security. Fortunately, peptides from animal venoms have emerged as potential drugs for the therapeutic care of cardiovascular diseases. This paper aims to investigate the therapeutic potentials and mechanism of bee venom (BV) using isoproterenol (ISO)-induced CH models in vivo and in vitro.

PEELing: an integrated and user-centric platform for spatially resolved proteomics data analysis.

Summary: Molecular compartmentalization is vital for cellular physiology. Spatially resolved proteomics allows biologists to survey protein composition and dynamics with subcellular resolution. Here, we present PEELing, an integrated package and user-friendly web service for analyzing spatially resolved proteomics data.

Gastric Acid-Protective and Intestinal Targeted Nanogels Enable Anti-Bacterial Activity of Cefquinome.

To enhance the antibacterial activity of cefquinome (CEF) against , a Carboxymethylcellulose sodium (CMCNa)/D-Mannosamine hydrochloride (DMH)-based nanogels delivery system capable of protecting CEF from gastric acid degradation while enabling intestinal sustained release and targeted antibacterial enhancement was developed. Systematic research was conducted on the best formulation, physicochemical characteristics, stability, gastrointestinal fluid-responsiveness, and antibacterial activity of the optimal formulation. The results showed that the optimized CEF nanogels demonstrated an enhanced loading capacity (13.

Comparative effectiveness of lifestyle interventions for prehypertension: A network meta-analysis of randomized controlled trials.

This study aims to compare the effectiveness of lifestyle interventions on systolic blood pressure (SBP) and diastolic blood pressure (DBP) in individuals with prehypertension. A systematic literature search was conducted in PubMed, Web of Science, Embase, Cumulative Index to Nursing and Allied Health Literature, China National Knowledge Infrastructure, and the Wanfang database from inception to December 2023. The analysis included randomized controlled trials with either parallel or cross-over designs.

Structure and Function Features of Abundant and Rare Prokaryotic Communities Along Nearshore to Offshore Transitions.

Abundant and rare taxa are crucial members of the marine microbial community. However, their biodiversity, assembly mechanisms, functional characteristics and ecological response strategies remain poorly understood. In this study, 16S rRNA and metagenomic sequencing were carried out to reveal the structural and functional features of abundant and rare taxa across the transition from nearshore to offshore.

Comprehensive Analysis of Faba Bean AP2/ERF Genes Suggests Potential Roles of VfAP2-1 and VfERF-99 in Abiotic and Biotic Stress Responses.

The AP2/ERFs not only participate in regulating signal networks, but they also play important roles in the process of plant growth and stress response. However, systematic research of AP2/ERF in Vicia faba is lacking. In this study, VfAP2/ERF was systematically identified and their characteristics were comprehensively analyzed.

β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins.

Glioblastoma multiform (GBM) constitutes approximately 14.7 % of all central nervous system tumors (CNSTs) and 45.2 % of primary malignant CNSTs.

Significance of glutathione in the hormesis effect: a case study of the relationship between heavy metal Cd and monitoring plant Tillandsia ionantha.

With the impact of human activities on the global environment intensifies, how different organisms adapt to environmental stressors has become a critical issue, especially to the most toxic heavy metals. Hormesis effect is an effective way to adapt to the harsh environmental changes, and glutathione (GSH) has long been considered to play an important role in the generation of hormesis, but the underlying mechanisms have not been thoroughly investigated. This experiment selected heavy metal monitoring plant Tillandsia ionantha as the experimental material and subjected it to cadmium (Cd) stress with different time gradients.

NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation.

Intervertebral disc degeneration (IVDD) is a prevalent condition leading to low back pain. Endoplasmic reticulum stress (ERS) is strongly linked to IVDD progression, although the underlying mechanisms remain unclear. In this study, we investigated the effects of NOXA on ERS-induced IVDD.

Carbon-supported uniform RuAgW nanoparticles with high activity and CO resistance toward alkaline hydrogen oxidation.

RuAgW/C demonstrates an excellent mass specific exchange current density () of 442.9 A g superior to that of Pt/C (329.7 A g) toward the alkaline hydrogen oxidation reaction (HOR) primarily owing to weakened hydrogen binding energy (HBE) and strengthened hydroxide binding energy (OHBE).

Human Cytomegalovirus-IE1 induces neonate liver injury in transgenic mouse.

Congenital human cytomegalovirus (HCMV) is a significant contributor to liver damage in infants and children. HCMV-encoded IE1 protein plays an important role in viral replication and disease progression. To investigate the effects IE1 on neonate liver, the transgenic mouse model (IE1 mice) and transcriptome sequencing were performed.

Root zone microbial communities of Krasch. at different successional stages in Mu US Sandy Land: a metagenomic perspective with culturomics insights.

Phytoremediation offers a promising strategy for addressing the global challenge of land desertification. In the Mu Us Sandy Land of China, Krasch. has emerged as a key species for desertification control.

Melatonin Promotes Osteogenic Differentiation of Rat Adipose-Derived Stem Cells via the p38/MAPK Signaling Pathway.

While adipose-derived stem cells (ADSCs) transplantation represents an appealing therapeutic strategy for bone defect repair, the osteogenic capacity of ADSCs is largely limited. Melatonin has been demonstrated to contribute to the bone marrow stem cell (BMSC) osteogenesis. However, its effect on the osteogenic differentiation of ADSCs has not yet been determined.

Mechanisms and Intervention Strategies of Trimethylamine N-Oxide in Abdominal Aortic Aneurysm.

Background: Abdominal aortic aneurysm (AAA) is a serious cardiovascular disease with high morbidity and mortality. The role of gut microbiota-derived metabolite trimethylamine N-oxide (TMAO) in AAA pathogenesis has attracted increasing attention.

Summary: This review systematically summarizes the mechanisms by which TMAO promotes AAA development, including inflammatory activation, apoptosis induction, and extracellular matrix degradation.

Survival Nomogram for Lung Adenocarcinoma Patients With Bone Metastasis Based on the SEER Database and an External Validation Cohort.

Background: Lung adenocarcinoma is a common type of cancer that can lead to bone metastasis and has a poor prognosis. Although previous studies have established nomograms for lung adenocarcinoma, these nomograms do not effectively predict the prognosis of lung adenocarcinoma patients with bone metastasis. This study aims to establish and validate a new nomogram to solve this problem.

Human cytomegalovirus-IE2 suppresses antigen presentation of macrophage through the IL10/STAT3 signalling pathway in transgenic mouse.

Human cytomegalovirus (HCMV) has evolved sophisticated strategies to evade host immune defenses, enabling its persistent survival in human populations. HCMV intermediate-early protein 2 (IE2) has been identified as a crucial factor in immune evasion mechanisms. However, the specific immunomodulatory effects of IE2 on antigen presentation remain insufficiently explored.

Predictive value of the preoperative C-reactive protein-to-albumin ratio for surgical site infection after percutaneous kyphoplasty: a single-center retrospective study.

Objective: This study aimed to investigate the risk factors for surgical site infection (SSI) after percutaneous kyphoplasty (PKP) and evaluate the application value of the preoperative C-reactive protein (CRP)-to-albumin ratio (CAR) in predicting SSI.

Methods: This study retrospectively enrolled 329 patients with thoracolumbar compression fractures who underwent PKP in the Affiliated Hospital of Qingdao University from January 2019 to June 2024. The demographic information, surgery-related data and laboratory examination results of the patients were collected.

High-throughput sequencing reveals microbial community dynamics in two treatment systems for oil field-produced water.

Petroleum-associated water harbors diverse microbial communities, including hydrocarbon-degrading bacteria, sulfate-reducing bacteria, and methanogenic archaea. The growth and metabolism of these organisms, as well as their community composition, can affect various aspects of oil field development and oil produced water treatment. In this study, Illumina-based 16S rRNA gene sequencing was used to analyze the microbial community structures of oilfield produced water processed at two treatment stations and subjected to different treatment protocols.

Accelerating enrichment of ARGs and MGEs with increasing ammonium removal during partial nitrification treating high-strength ammonia wastewater.

Free ammonia (FA) and free nitrous acid (FNA) are critical operational parameters to maintain partial nitrification (PN) in treating high-strength ammonia wastewater. However, whether FA and FNA, as non-antibiotic antimicrobial chemicals, could directly enhance antibiotic resistance genes (ARGs) enrichment during PN is still unclear. This study deciphered the prevalence of ARGs, potential mobility, and hosts under different ammonium removal efficiencies (ARE) (different FA and FNA) during stable PN.

Intricate relationship among major heat-induced harmful by-products and modulating role of lipid and protein oxidation in seafood: A comprehensive review.

Seafood is an important constituent of our diet and well-known for its high-quality proteins and lipids (especially polyunsaturated fatty acids). While thermal processing imparts unique flavors to seafood, it also generates heat-induced harmful by-products (HHBs), such as polycyclic aromatic hydrocarbons (PAHs), heterocyclic aromatic amines (HAAs), and advanced glycation end-products (AGEs), which pose a serious threat to human health in the long run. This manuscript provides an overview of the formation mechanisms of PAHs, HAAs, and AGEs, and their occurrence in seafood.

PURPL Represses Radiation-Induced Apoptosis to Promote Radioresistance in Cutaneous Melanoma by Direct Interfering With BID Cleavage.

The rise of radioresistance in treating cutaneous melanoma challenges the efficacy of radiotherapy. Transcriptomic sequencing highlights PURPL as one of the top upregulated long noncoding RNAs in response to ionizing radiation (IR) treatment in melanoma cells, suggesting its role in radioresistance. To explore such hypothesis, loss-of-function experiments were conducted to assess the impact of PURPL on melanoma cell viability, colony formation, and migration.