Creating Abundant Open Metal Sites in MOFs by Dual-Coordination Design for Enhanced Electrocatalytic Activity.

Metal-organic frameworks (MOFs) with well-defined crystalline structures offer ideal platforms to unravel structure-property relationships, but their low density of accessible metal sites limits catalytic activation. Efforts to generate open metal sites often compromise structural integrity, obstructing mechanistic investigation. In this study, we convert single-coordinated MOFs into dual-coordinated frameworks, enabling controlled creation of unsaturated metal sites to boost electrocatalytic performance while preserving crystal framework for structure-property study.

Optimized Cell-Based Platform for Platelet-Free Detection of Heparin-Induced Thrombocytopenia Antibodies.

Accurate detection of heparin-induced thrombocytopenia (HIT) antibodies is crucial for diagnosing and managing thrombotic events. Conventional immunoassays, however, often lack specificity and require confirmatory testing with fresh human platelets. To address this limitation, we optimized our previously developed cell-based enzyme-linked immunosorbent assay (ELISA) for improved HIT detection under various experimental conditions.

Molecular Insights into the Interaction between CD147 and the SARS-CoV‑2 Spike Protein.

Cluster of differentiation 147 (CD147), a transmembrane glycoprotein, has been identified as a potential auxiliary receptor for the SARS-CoV-2 spike protein (SP), contributing to COVID-19 infection. However, the detailed binding characteristics of this interaction remain unclear. Here, we characterized SP-CD147 binding using Enzyme-Linked Immunosorbent Assay (ELISA) and single-molecule force spectroscopy (SMFS) under varying contact times and temperatures.

SynchroSep-MS: Parallel LC Separations for Multiplexed Proteomics.

Achieving high throughput remains a challenge in MS-based proteomics for large-scale applications. We introduce SynchroSep-MS, a novel method for parallelized, label-free proteome analysis that leverages the rapid acquisition speed of modern mass spectrometers. This approach employs multiple liquid chromatography columns, each with an independent sample, simultaneously introduced into a single mass spectrometer inlet.

Parental educational levels association with autism risk in offsprings and their autistic phenotypes.

This study aims to explore the association between parental education levels and the risk of autism in offspring, as well as to further investigate the relationship between parental education and the phenotypic characteristics of autistic symptoms. The study is based on an autism cohort of 835 autistic children, with a control group of 504 typically developing children randomly selected for comparison. Socioeconomic data from the parents of the study participants were collected, and the clinical phenotypes of the autistic children were assessed.

Multifaceted roles of gene family in flowering, plant architecture, and adaptation.

gene family encodes transcription factors with a basic region/leucine zipper (bZIP) domain that play an essential role in floral transition regulation, which is vital for plants' reproduction. Recent studies have uncovered additional functions for gene family in plant development, hormone signaling, and response to environmental cues. These pleiotropic roles make them promising targets for modern crops' breeding.

Evaluation of the effects of different formulations of protectants on the preservation of the microbiota in fecal microbiota transplantation.

Background: With the increasing indications for fecal microbiota transplantation for the treatment of diseases, there is a growing demand for the preparation of frozen or lyophilized fecal microbiota products that are viable and can stably colonize the recipient. The addition of protective agents plays an important role in the preparation. However, there has been no systematic evaluation of the protective agents used in fecal microbiota sample transplantation preparation for transplantation.

Evaluation and reinforcement of the physicochemical properties of hybrid meatball kung-wan partially replaced with rice protein.

Hybrid meat products, involving the partial replacement of meat with plant proteins, provide a promising solution to address the rising consumer demand for sustainable and healthier food options. Rice protein (RP), featuring balanced amino acid composition and hypoallergenic property, represents a potent alternative to common legume-based proteins. This study investigates the potential of RP as a partial replacer for pork in kung-wan, a traditional Oriental-style emulsified meatball.

Dual prophylactic and therapeutic potential of iPSC-based vaccines and neoantigen discovery in colorectal cancer.

Induced pluripotent stem cells (iPSCs) share transcriptomic similarities with cancer cells and express tumor-specific and tumor-associated antigens, highlighting their potential as cancer vaccines. Our previous study demonstrated that an iPSC-based vaccine effectively prevented tumor growth in various mouse models, including melanoma, breast, lung, and pancreatic cancers. However, the underlying mechanisms and the therapeutic efficacy of the iPSC-based vaccine remain unclear.

Multimodal GPT model for assisting thyroid nodule diagnosis and management.

Although using artificial intelligence (AI) to analyze ultrasound images is a promising approach to assessing thyroid nodule risks, traditional AI models lack transparency and interpretability. We developed a multimodal generative pre-trained transformer for thyroid nodules (ThyGPT), aiming to provide a transparent and interpretable AI copilot model for thyroid nodule risk assessment and management. Ultrasound data from 59,406 patients across nine hospitals were retrospectively collected to train and test the model.

Dual-atomic Cu-Ag pairs boosting selective electroreduction of CO to acetate.

Single-atomic Cu catalysts show promise for the electrochemical CO reduction (CORR) to acetate, but their efficiency is limited by the difficulty in generating the CO intermediate needed for C-C coupling. While co-catalysts can enhance CO generation, weak interaction between co-catalytic and single-atom Cu sites hinders CO spillover, resulting in low acetate yield. Herein, we design atomic Cu-Ag pairs to enhance CO generation and facilitate CO spillover from Ag to Cu in the CORR to enhance acetate production.

Edge-hosted Fe single-atomic sites fabricated by a Bi-assisted pyrolysis strategy for electroreduction of CO.

We develop a Bi-assisted pyrolysis method to fabricate rich-edge N-doped carbon anchored with Fe active sites using Fe-doped Bi-MOF as a precursor. During pyrolysis, Bi aggregation forms edge-rich nanobelts, while the subsequent Bi evaporation generates mesopores, increasing edge-hosted Fe site density. The resulting catalyst exhibits superior intrinsic activity and selectivity for CO-to-CO, outperforming in-plane Fe single sites.

Exosomal biomarkers: A novel frontier in the diagnosis of gastrointestinal cancers.

Gastrointestinal (GI) cancers, which predominantly manifest in the stomach, colorectum, liver, esophagus, and pancreas, accounting for approximately 35% of global cancer-related mortality. The advent of liquid biopsy has introduced a pivotal diagnostic modality for the early identification of premalignant GI lesions and incipient cancers. This non-invasive technique not only facilitates prompt therapeutic intervention, but also serves as a critical adjunct in prognosticating the likelihood of tumor recurrence.

Halting hepatocellular carcinoma: Identifying intercellular crosstalk in HBV-driven disease.

Hepatitis B infection can lead to liver fibrosis and hepatocellular carcinoma (HCC). Despite antiviral therapies, some patients still develop HCC. This study investigates hepatitis B virus (HBV)-induced hepatocyte-hepatic stellate cell (HSC) crosstalk and its role in liver fibrosis and HCC.

Haem biosynthesis regulates BCAA catabolism and thermogenesis in brown adipose tissue.

The distinctive colour of brown adipose tissue (BAT) is attributed to its high content of haem-rich mitochondria. However, the mechanisms by which BAT regulates intracellular haem levels remain largely unexplored. Here we demonstrate that haem biosynthesis is the primary source of haem in brown adipocytes.

Evening complex component ELF3 interacts with LUX proteins to repress soybean root nodulation.

Formation of root nodules is a unique hallmark of the symbiotic interaction between legume host plants and rhizobia and is governed by a complex regulatory framework that balances the appropriate orchestration of rhizobial infection and subsequent nodule organogenesis. In contrast to prominent model species such as Medicago truncatula and Lotus japonicus, research on symbiotic signal transduction in the staple-crop soybean Glycine max remains relatively insufficient. Here, we identified a soybean mutant with ~25% additional root nodules over wild-type, designated as increased number of nodules 1 (inn1).

[Investigation of the Reliability and Validity of the Chinese Version of the Beck Depression Inventory-Ⅱ in Middle School Students of Yi Ethnicity in Liangshan Prefecture, China].

Objective: To assess the reliability and validity of the Chinese version of the Beck Depression Inventory, Second Edition (BDI-Ⅱ-C) among Yi middle school students in Liangshan Prefecture.

Methods: A stratified cluster sampling method was used to select 466 Yi middle and high school students in Liangshan Prefecture to respond to the BDI-Ⅱ-C. The general demographic characteristics of the samples were described, and independent sample -test, chi-square test, and reliability analysis were conducted to analyze the data.

Age-stratified deep learning model for thyroid tumor classification: a multicenter diagnostic study.

Objectives: Thyroid cancer, the only cancer that uses age as a specific predictor of survival, is increasing in incidence, yet it has a low mortality rate, which can lead to overdiagnosis and overtreatment. We developed an age-stratified deep learning (DL) model (hereafter, ASMCNet) for classifying thyroid nodules and aimed to investigate the effect of age stratification on the accuracy of a DL model, exploring how ASMCNet can help radiologists improve diagnostic performance and avoid unnecessary biopsies.

Methods: In this retrospective study, we used ultrasound images from three hospitals, a total of 10,391 images of 5934 patients were used for training, validation, and testing.

Monkeypox outbreak 2023 in Chengdu, China: an observational study.

Monkeypox is a zoonotic viral disease caused by monkeypox virus infection and formerly this disease had been endemic mainly in Africa. Since May 2022, the disease has rapidly spread across the world and some imported case was confirmed sporadically in China. Under these circumstances, data of confirmed cases were collected consecutively in Chengdu, southwest China to investigate clinical and epidemiological characteristics.

Promoting Hydrogen Transfer in Electrochemical CO Reduction via a Hydrogen on Demand Pathway.

The proceeding of electrochemical CO reduction reaction (CORR) requires the formation of active hydrogen species for CO protonation, while traditional catalysts fail to balance the rate of hydrogen supply and CO protonation. Herein, we propose a "hydrogen on demand" mechanism, in which the polarity of the adsorbed CO is enhanced to allow the capture of hydrogen from water without forming free hydrogen species, realizing the matching rate of hydrogen supply and CO protonation. As a proof of concept, we construct Zn-N sites modified by Se atoms, allowing the proceeding of CORR under the "hydrogen on demand" mechanism with superior efficiency.

Selective Electroreduction of CO to CO over Ultrawide Potential Window via Implanting Active Site with Long-Range P Regulation on Periodic Pores.

Electroreduction of CO to CO represents a highly promising way for artificial carbon cycling, but obtaining high selectivity over a wide potential window remains a challenge due to the sluggish CO generation and diffusion kinetics. Here we report an integration of long-range P modified bismuth atomic site on an ordered macroporous carbon skeleton with mesoporous "wall" (MW-BiN-POMC) for efficient electroreduction of CO. In-depth in situ investigations with theoretical computations reveal that the incorporation of long-range P atom is able to strengthen the orbital interaction between the C 2p of CO and Bi 6p, thereby establishing an electronic transport bridge for the activation of CO molecule.

Case management strategy enhances virological response in people living with human immunodeficiency virus in a resource-limited region in Southwest China: a real-world prospective observational study.

This study assessed the effectiveness of case management compared to that of standard care in improving treatment outcomes for first-time antiretroviral therapy (ART) patients in Liangshan Prefecture, China. First-time ART patients (total n = 828) were divided into the Standard Care Group (SCG, n = 419) and Case Management Group (CMG, n = 409). At week 48, the CMG showed higher retention rates (97.