Adaptation and psychometric evaluation of the Chinese version of the hip disability and osteoarthritis outcome Score-Physical function Short-form (HOOS-PS).

Background: Chronic hip joint disease has a high prevalence in China, and the primary scale currently used to assess functional recovery after total hip arthroplasty (THA) is the Hip disability and Osteoarthritis Outcome Score - Physical Function Short-form (HOOS-PS). Therefore, our objective was to translate the HOOS-PS scale into Simplified Chinese and evaluate its reliability, validity, and responsiveness in THA patients.

Methods: First, we followed the widely accepted cross-cultural translation process to translate the original HOOS-PS into the Chinese version of HOOS-PS (CHOOS-PS).

Determinants of childcare service demand for infants aged 0-3 among the childbearing population in China.

Objectives: This study aims to investigate the demand for childcare services for infants aged 0-3 years among the childbearing population in China and identify its key determinants.

Methods: An online survey was conducted in Suzhou, China in August 2024 using a self-designed questionnaire. Information on personal and family characteristics, as well as demand for childcare services, was collected.

High-Performance Electrochemiluminescence Imaging Immunosensor for Adeno-Associated Virus Serotype 8 Detection Based on Nanobody-Functionalized Covalent Organic Frameworks and Au@Rh Catalytic Amplification.

Adeno-associated virus serotype 8 (AAV8) is a widely used gene therapy vector with the characteristics of high transduction efficiency and tissue specificity. Reliable detection of AAV8 is crucial for assessing therapeutic efficacy and tracking its in vivo distribution. Herein, we develop a nanobody-based electrochemiluminescence (ECL) imaging immunosensor based on integrating advanced functional materials with spatially resolved signal output for sensitive and visual detection of AAV8.

Low-triggering-potential electrochemiluminescence biosensor based on hydrogen-bonded organic framework and luminol-derived carbon dots for α-glucosidase detection.

Low-triggering-potential can decrease electrochemical interference and cross talk of electrochemiluminescence (ECL), significantly improving long-term stability of working electrode. Herein, we develop a ratiometric ECL biosensor with hydrogen organic framework (HOF) and luminol derived carbon dots (Lu-CDs) as the emitters for sensitive detection of α-glucosidase (α-Glu) at a low positive potential range of 0.1-1.

Genomic insights into the reticulate evolution of viviparous Phrynocephalus lizards on the Qinghai-Tibetan Plateau.

The contribution of hybridization to the speciation and diversification of organisms remains a topic of ongoing debate. While ancient reticulate events have been increasingly recognized as a common feature in evolutionary history of many species, the role of hybridization in driving adaptation, especially in extreme environments, is still unclear. The viviparousPhrynocephaluslizards present an excellent system to investigate this question, which are distributed across the Qinghai-Tibetan Plateau (QTP) with most species occurring above 2,300 m above sea level.

ROS confer resistance to 3TC in p53 mutant colorectal cancer by promoting autophagy and ER stress.

Colorectal cancer (CRC) is among the most frequently diagnosed cancers globally. Lamivudine (3 TC), a nucleoside reverse transcriptase inhibitor (NRTI) commonly used for treating Human Immunodeficiency Virus (HIV) and Hepatitis B Virus (HBV), has recently demonstrated anticancer activity against p53-mutant CRC in Phase 2 clinical trials. However, the underlying mechanisms remain elusive.

Fructose metabolism in Entner-Doudoroff pathway-deficient Cupriavidus necator H16 depends on the Calvin shunt.

As a facultative chemolithoautotrophic bacterium, Cupriavidus necator H16 uses the Entner-Doudoroff (ED) pathway for heterotrophic growth on carbohydrates such as fructose and the Calvin cycle for lithoautotrophic carbon dioxide fixation. In a previous study, we found that an ED pathway-deficient C. necator strain can survive on fructose, but the underlying metabolic pathway remained unclear.

The Structure, Assembly Processes of Microbial Communities and Their Effects on the Quality of Goat MEAT During Chilled Storage (4 °C).

Microbial community succession is closely related to the corruption of meat, but there are few studies on microbial community assembly and their relationship with physicochemical indexes in meat during chilled storage (4 °C). This study aimed to investigate the mechanism of bacterial community assembly and the effect of microbial succession on quality changes during the preservation of goat meat. The results showed that the stochastic process was the primary driving mechanism during community construction.

Short-term comparison of switching to faricimab from other anti-VEGF agents in neovascular age-related macular degeneration patients: A retrospective study.

In order to evaluate the short-term outcomes of switching to faricimab from other anti-vascular endothelial growth factor (VEGF) agents in Chinese patients with neovascular age-related macular degeneration (nAMD). This was a retrospective, observational study involving patients with nAMD who had insufficient response to previous anti-VEGF therapy and were switched to Faricimab. Best-corrected visual acuity, central macular thickness, and pigment epithelium detachment (PED) changes were recorded at baseline and after one month of treatment.

Identification of a novel linear B-cell epitope recognized by a monoclonal antibody against the capsid protein of goose astrovirus genotypes 2.

Background: Goose astrovirus genotype 2 (GoAstV-2) is a novel and highly infectious pathogen characterized by symptoms of gout, swelling, and hemorrhage of the kidneys in 5-15-day-old goslings. The virus has caused severe economic losses for the goose husbandry industry. The capsid protein of GoAstV-2 is involved in viral packaging and immunogenicity, and it has served as a target antigen for diagnostic methods.

Anti-fibrosis effect of astragaloside IV in animal models of cardiovascular diseases and its mechanisms: a systematic review.

Context: Myocardial fibrosis is a common manifestation of end-stage cardiovascular disease, but there is a lack of means to reverse fibrosis. Astragaloside IV (AS-IV), the major active component of Fisch. ex Bunge Fabaceae, possesses diverse biological activities that have beneficial effects against cardiovascular disease.

Parents' perceptions of physical activity for their children with cancer: a qualitative meta-synthesis.

Background: The the number of cases of cancer in children is increasing annually. Physical activity (PA) can enhance the future outcomes and quality of life of children with cancer. However, studies have shown that children with cancer have low levels of PA and that the majority don't adhere to the guidelines' recommendations for physical activity.

Design of Ni-FAU Zeolite Bifunctional Materials for Integrated Carbon Dioxide Capture and Methanation: Construction of ultrafine NiO nanoparticles.

The Integrated Carbon Dioxide (CO) Capture and Methanation (ICCU-Met) technology has emerged as a promising strategy for reducing CO emissions while producing methane (CH) fuel. However, a significant challenge in this process is the absence of bifunctional materials (DFMs) that simultaneously possess highly dispersed metal sites and stable adsorbent structures under variable temperature conditions. This is particularly problematic because existing nickel-based (Ni) bifunctional materials are prone to metal sintering and structural degradation at high temperatures.

Astragaloside IV Attenuates Angiotensin II-Induced Inflammatory Responses in Endothelial Cells: Involvement of Mitochondria.

Background: Angiotensin II (Ang II)-triggered endothelial inflammation is a critical mechanism contributing to Ang II-related cardiovascular diseases. The inflammation is highly correlated with mitochondrial function. Although astragaloside IV (AS-IV), a primary bioactive ingredient extracted from the traditional Chinese medicine Bunge that can effectively treat numerous cardiovascular diseases, posses the actions of antiinflammation and antioxidation in vivo, limited data are made available on the impacts of AS-IV on mitochondrial function in endothelial inflammation triggered by Ang II.

Enhanced Electrochemiluminescence of Porphyrin-Based Hydrogen-Bonded Organic Frameworks at Low Positive Potential via Substituent-Induced Outer-Sphere Microenvironment Modulation.

The porphyrin-based hydrogen-bonded organic framework (HOF) offers a superior platform for decoding electrochemiluminescence (ECL) via controlling charge transfer due to its higher solubility, chemical stability, and tunable framework behavior. In this research, three kinds of HOFs including TDPP-HOF, TCPP-HOF, and TCNPP-HOF are synthesized based on a porphyrin tectonic plate decorated with 2,4-diaminotriazinyl (DAT), carboxyl, and nitrile moieties to study their ECL performances. The hydrazine as the coreactant can trigger TDPP-HOF at the low-excited positive potential to generate 15.

Construction of a Self-Enhanced Electrochemiluminescent Sensor Based on Tandem Signal Amplification and a Self-Luminescent Lanthanide Covalent-Organic Polymer for Ochratoxin A Assay.

Ochratoxin A (OTA) is a type of mycotoxin found in various contaminated foods, and it is highly toxic to the livers and kidneys of humans. Herein, we develop a self-enhanced electrochemiluminescent (ECL) sensor based on tandem signal amplification and a self-luminescent europium covalent-organic polymer (Eu-COP) for OTA assay. Eu-COP is a self-enhanced ECL emitter that is obtained by using 2,4,6-trimethyl-1,3,5-triazine (TMT) and 2,2'-bipyridine-5,5'-dicarbaldehyde (BPA) as the first and second ligands to form a highly conjugated structure for sensitizing the Eu luminescence.

Constructing SiO-Supported Atomically Dispersed Platinum Catalysts with Single-Atom and Atomic Cluster Dual Sites to Tame Hydrogenation Performance.

Construction and optimization of stable atomically dispersed metal sites on SiO surfaces are important yet challenging topics. In this work, we developed the amino group-assisted atomic layer deposition strategy to deposit the atomically dispersed Pt on SiO support for the first time, in which the particle size and ratio of Pt entities from single atom (Pt) to atomic cluster (Pt ) and nanoparticle (Pt ) on the SiO surface were well modulated. We demonstrated the importance of dual-site synergy for optimizing the activity of single-atom catalysts.

Recent advance for enantiorecognition of chiral drugs sensing: Electrochemical, electrochemiluminescent and photoelectrochemical application.

Chiral isomers show different behaviours and properties in physiological activities. It is of great significance to find productive approach to realize the recognition of enantiomers, which is a key issue in biochemical and pharmaceutical fields. Nowadays, chiral identification can be successfully achieved according to the discrepancies of special signals correlated with different enantiomers of multiple electrode structures.

Atypical conjunctival sporotrichosis secondary to Mooren's ulcer: a case report.

Background: Conjunctival sporotrichosis is a rare fungal infection, typically presenting as granulomatous lesions. Its manifestations can be atypical, particularly in immunosuppressed patients. Here, we present a rare case of a Mooren's ulcer patient with bulbar conjunctival Sporotrichosis presenting as a salmon-pink tumor.

Remarkable increase in electrochemiluminescence of isomeric bipyridine-based covalent organic frameworks via regulating the direction of imine linkage for sensing application.

Covalent organic frameworks (COFs) with highly ordered structures and predictable optoelectronic properties provide an ideal platform to investigate the electrochemiluminescence (ECL) performance based on organic materials by atomically varying the molecular construction. Herein, the effect of imine-bond orientation on the ECL performance of COFs is investigated. We report two COFs (NC-COF and CN-COF) with different orientations of imine bonds using pyrene donor units (D) and bipyridine acceptor motifs (A) monomers.

Singapore's COVID-19 Genomic Surveillance Programme: Strategies and Insights From a Pandemic Year.

Background: During the COVID-19 pandemic, genomic surveillance was crucial for monitoring virus spread and identifying variants. Effective surveillance helped understand transmission dynamics. Singapore had success in combating COVID-19 through its surveillance programmes.

Absolute quantification of Neuron-specific enolase based on surface plasmon resonance.

Neuron-specific enolase (NSE) is currently the most reliable biomarker for small cell lung cancer (SCLC), which is important for disease monitoring, clinical evaluation and diagnosis. However, traditional methods suffer from various disadvantages, including instability, complexity, time-consuming operations, and the necessity for standards. In this study, we developed a calibration-free concentration analysis (CFCA) method based on surface plasmon resonance (SPR) technology, to accurately quantify the active concentration of NSE without relying on any standards.

Childhood acute flaccid myelitis, including the first confirmed cases of enterovirus D68 myelitis, in Singapore and Southeast Asia.

Aim: We report the epidemiology, treatment and outcomes of acute flaccid myelitis (AFM), including the first two cases of enterovirus (EV) D68 myelitis, in Singapore.

Methods: Retrospective observational study from a paediatric hospital in Singapore, from January 2012 to December 2022. Clinical, laboratory, neuroimaging and outcome data were analysed.