p300/CBP is an essential driver of pathogenic enhancer activity and gene expression in Ewing sarcoma.

The t(11;22) translocation encodes the EWS::FLI1 fusion oncoprotein which is the primary driver of Ewing sarcoma. EWS::FLI1 creates unique, de novo pathogenic enhancers that drive gene expression and are a central mechanism of oncogenesis. Which chromatin regulatory proteins are critical to this mechanism is understudied.

Loss of NOTCH2 creates a TRIM28-dependent vulnerability in small cell lung cancer.

Small cell lung cancer (SCLC) is a highly aggressive malignancy that lacks effective targeted therapies, in part due to frequent loss-of-function mutations in tumor suppressors and the absence of recurrent oncogenic drivers. Approximately 15% of SCLCs harbor inactivating mutations in NOTCH1 or NOTCH2, and most neuroendocrine-high SCLCs exhibit low NOTCH activity. Using CRISPR-Cas9 screening in primary cell lines derived from NOTCH1/2-isogenic SCLC genetically engineered mouse models, we identified TRIM28 as a synthetic lethal dependency in NOTCH2-inactivated SCLCs.

DistilCLIP-EEG: Enhancing Epileptic Seizure Detection Through Multi-modal Learning and Knowledge Distillation.

Epilepsy is a prevalent neurological disorder marked by sudden, brief episodes of excessive neuronal activity caused by abnormal electrical discharges, which may lead to some mental disorders. Most existing deep learning methods for epilepsy detection rely solely on unimodal EEG signals, neglecting the potential benefits of multimodal information. To address this, we propose a novel multimodal model, DistilCLIP-EEG, based on the CLIP framework, which integrates both EEG signals and text descriptions to capture comprehensive features of epileptic seizures.

Unveiling the therapeutic mechanisms of Saorilao-4 decoction in pulmonary fibrosis through metabolomics and transcriptomics.

Pulmonary fibrosis (PF) is a challenging and intricate lung ailment to treat. Saorilao-4 decoction (SRL) is a traditional Mongolian medicine remedy frequently utilized in the management of lung disorders. This study employed transcriptomics and metabolomics to explore the molecular mechanisms of SRL in the bleomycin-induced PF rat model.

ALKBH5-mediated mA demethylation modification of RAD51 inhibits osteogenic differentiation via promoting DNA damage in osteoporosis.

Osteoporosis (OP) is a metabolic disorder characterized by reduced bone mineral density and degeneration of bone tissue microarchitecture. Osteogenic differentiation plays a pivotal role in OP pathogenesis by facilitating new bone formation, preserving bone strength and density, and counteracting bone resorption. RNA epigenetic modifications have been increasingly implicated in multiple aspects of bone metabolism.

Comprehensive Review-Chemistry and Bioactivities of Secondary Metabolites From Chaetomium globosum.

Chaetomium sp. is a widely distributed fungal genus that has been identified as a source of chemically diverse and biologically active natural products. This review aimed to comprehensively survey secondary metabolites derived from Chaetomium globosum, including chemical diversity, biological activities, and related pathways of biosynthesis and chemosynthesis.

Effect of low-energy stirring temperature on the formation of oil-in-water emulsions stabilized by gelatin-arginine complexes.

The impact of low-energy stirring temperatures (30 °C, 50 °C, 70 °C, 90 °C) on the stability of gelatin emulsions was investigated when stirring with a magnetic stirrer at 1600 rpm for 3.5 h at an arginine (Arg) concentration of 1.2 % (w/v).

Fetal umbilical-portal-systemic venous shunt diagnosed by prenatal ultrasonography and its association with chromosomal abnormalities.

Background: Fetuses with umbilical-portal-systemic venous shunt (UPSVS) have poor prognosis, and standard prenatal ultrasound planes do not contain the umbilical-portal-systemic venous system. Moreover, UPSVS-related research is limited, and the rate of chromosomal abnormalities remains unknown. It is necessary to further clarify the ultrasound characteristics and prognosis of these fetuses and develop effective diagnostic models.

Activation of a nongenetic AHR-ELMSAN1 axis optimizes BET-targeting therapy and suppresses leukemia stem cells in preclinical models.

Developing strategies to enhance the response to bromodomain and extraterminal domain (BET) inhibitors and effectively eradicate cancer stem cells would represent a major cancer treatment advance against leukemia. Through a functional CRISPR screen, we identified the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, as a critical regulator of MYC expression and BET inhibitor sensitivity in human acute myeloid leukemia (AML). Constitutive or pharmacological activation of AHR repressed MYC and synergized with BET inhibitors to inhibit MYC transcription and suppress leukemia growth across diverse AML models.

Effects of vegetable extracts on the formation of heterocyclic aromatic amines and advanced glycation end products during roasting and in vitro digestion of roasted beef patties.

The research explored the effect of tomato, carrot, and lettuce extracts on the formation of heterocyclic aromatic amines (HAAs) and advanced glycation end products (AGEs) in beef patties during roasting and in vitro digestion. Carrot extract had strongest inhibitory effects on HAAs and AGEs formation, reducing total free HAAs (21.01 %), free AGEs (12.

The effect and mechanism of sublethal injury by lactic acid on Salmonella Typhimurium planktonic and biofilm cells.

Salmonella is a zoonotic pathogen present in the food and environment, which could survive as sublethally injured form during treatment. The effects of sublethal injury by lactic acid (LA) on S. Typhimurium planktonic and biofilm cells in broth, food manufacturing wastewater, and aqueous solution were investigated in this study.

Decellularized cartilage scaffolds derived from wharton's jelly facilitate cartilage regeneration and inhibit angiogenesis.

The avascular nature of articular cartilage severely limits its ability to self-repair after injury, which poses a challenge for clinical treatment, and tissue engineering aims to address this issue with scaffold-based strategies. However, the defining characteristics of an optimal scaffold remain controversial. In this study, we prepared two types of decellularized wharton's jelly (dWJ) scaffolds by trypsin combined with repeated freeze-thawing (TFT) and nuclease combined with repeated freeze-thawing (NFT), respectively.

Chemical Perturbations Impacting Histone Acetylation Govern Colorectal Cancer Differentiation.

Background & Aims: Aberrant epigenetic programs that suppress differentiation and enhance plasticity drive colorectal cancer (CRC), yet the molecular determinants underlying these processes remain elusive. We aimed to identify and characterize epigenetic regulators of CRC differentiation, uncovering mechanisms that reprogram cancer cell states.

Methods: A small-molecule library targeting epigenetic regulators was screened using an endogenous dual-reporter system.

The role of mA RNA methylation in a love-hate relationship between porcine rotavirus and host cells.

N6-methyladenosine (mA), the most abundant mRNA modification, regulates various mRNA metabolism to affect numerous physiological processes, including immune response. Interestingly, many RNA viruses contain internal mA modifications that contribute to viral replication and innate immune escape process, but its mechanisms remain unclear. Porcine rotavirus (PoRV) is a common cause of diarrhea and gastroenteritis in piglets.

Design of a Universal Backbone Improving the Endonuclease Dependence Molecular Beacon Assay.

The endonuclease dependence molecular beacon assay (DEMBA) is an isothermal detection method celebrated for its high sensitivity, operational ease, and time-saving capabilities. However, the intricate design of the probes limits the extensive application of DEMBA. The main challenge is the requirement for the probe sequence to include restriction endonuclease sites, two target sequence binding sites, and self-binding sites, which frequently overlap.

Formation of aroma compounds in smoked chickens during the sugar-smoking process using SPME/SAFE-GC-MS and ESR technologies.

Free radicals play crucial roles in the development of grilled meat flavors, but the mechanisms by which they contribute to the formation of flavor compounds in smoked chickens remain unclear. In this study, GC-MS, ESR, and UPLC techniques were employed to analyze the dynamic changes in key flavor compounds, sugars, and free radicals in smoked chickens. A total of 22 (OAVs >1) key volatile compounds were identified in smoked chickens.

Inhibiting the expression of GPR43 in macrophages can alleviate osteoarthritis by suppressing the M1 polarization and suppressing ROS production.

G-protein-coupled receptor 43 (GPR43) plays an important role in immunomodulation, metabolic regulation and ROS production. Our research aims to explore the role and mechanism of GPR43 in osteoarthritis (OA). We experimentally determined that following the intervention of macrophages with lipopolysaccharide (LPS), there is a significant upregulation in the expression of GPR43.

Noninsulated Microneedle Radiofrequency for Skin Rejuvenation: A Histological, Transcriptomic and Clinical Study.

Background: The efficacy and safety of microneedle radiofrequency (MRF) for antiaging skin treatment has already been demonstrated. This study aims to, for the first time, comprehensively interpret the short and long-term effects of MRF by combining histology, high-throughput molecular analysis, and clinical evaluation.

Methods: At 1, 3, and 6 months after MRF in different parameters, the skin samples were taken for HE staining, Masson staining, and Victoria blue staining to observe the overall histological changes.

N-methyladenosine reader YTHDF3-mediated CEBPA translation maintains genomic stability and stem cell function to prevent liver injury.

Liver injury is a major health issue with significant implications for liver function and overall well-being, but precise mechanisms of the N-methyladenine (mA) reader YTHDF3 in liver injury remain severely understudied. Here, we discovered that Ythdf3 knockout exacerbated CCL-induced liver injury with a reduction in functional hepatocytes and liver stem cells using single cell RNA-sequencing and organoid culture. Furthermore, Mettl14 and YTHDF3-dependent RNA mA dysregulation induced DNA damage.

Cultured biomass enhances the flavor and nutrition characteristics of biomass/plant hybrid cultured meatballs.

Cultured biomass, as a novel food ingredient, significantly contributes to providing meat-like flavor and nutrition. In this study, cultured porcine fat biomass (hereafter referred to as "biomass") was used as a fat substitute in the biomass/plant hybrid cultured meatballs (BPMs) to investigate the effect of biomass levels (0 %, 10 %, 20 %, and 30 %) on the flavor and nutritional characteristics, compared to the FPM-10 % group containing 10 % pig subcutaneous fat. Results showed that increasing biomass levels significantly enhanced the protein, fat, and total amino acid content of BPMs (p < 0.

Risk Factors and Prognostic Implications in Synchronous Breast-Thyroid Dual Primary Cancers: A Matched Case-Control Study.

Objective: To characterize the distinct clinicopathological patterns and identify independent risk factors in synchronous breast-thyroid dual primary malignancies (DBTMs), a rare yet clinically significant entity.

Methods: In this retrospective case-control study (2019.03-2021.

Highly quantitative measurement of differential protein-genome binding with PerCell chromatin sequencing.

Quantitative comparison of ChIP-seq profiling between experimental conditions or samples remains technically challenging for the epigenetics field. Here, we report a strategy combining the use of well-defined cellular spike-in ratios of orthologous species' chromatin and a bioinformatic analysis pipeline to facilitate highly quantitative comparisons of 2D chromatin sequencing across experimental conditions. We find that the PerCell methodology results in efficient and consistent levels of spike-in vs.

Targeting transcription factors through an IMiD independent zinc finger domain.

Immunomodulatory imide drugs (IMiDs) degrade specific C2H2 zinc finger degrons in transcription factors, making them effective against certain cancers. SALL4, a cancer driver, contains seven C2H2 zinc fingers in three clusters, including an IMiD degron in zinc finger cluster one (ZFC1). Surprisingly, IMiDs do not inhibit the growth of SALL4-expressing cancer cells.

NN-based visual servoing compensation control of a Gough-Stewart platform with uncertain load.

This paper addresses the trajectory tracking control of a Gough-Stewart platform (GS platform) with an uncertain load. The uncertainty of this load leads to external disturbance to the parallel robot, which affects the dynamic coupling among the six degrees of freedom (DOF) and the tracking performance. Even though many researchers focus on improving the system robustness and tracking accuracy, there still exist two main problems: the system's internal uncertainties, including the modeling, manufacturing, and assembly errors of the parallel robot affect the control accuracy; the uncertain external disturbance varies in an extensive range and reduces the stability and tracking accuracy of the system.

The Application of scRNA-Seq in Heart Development and Regeneration.

Single-cell RNA sequencing (scRNA-seq) is a rapidly developing and useful technique for elucidating biological mechanisms and characterizing individual cells. Tens of millions of patients worldwide suffer from heart injuries and other types of heart disease. Neonatal mammalian hearts and certain adult vertebrate species, such as zebrafish, can fully regenerate after myocardial injury.