A strategy to re-sensitise drug-resistant Gram-positive bacteria to oxazolidinone-class antibiotics.

Background: Multidrug-resistant bacterial infections have high mortality rates and few treatment options. Synergistic combinations may improve clinical outcome but traditional strategies often damage healthy microbiome. Oxazolidinone-class antibiotics are typical last-resort drugs for treating drug-resistant bacterial infections but are becoming less effective due to resistance development.

The organelle tethering protein PDZD8 regulates endolysosomal maturation and TLR9-NF-κB signaling in cisplatin-induced acute kidney injury.

Acute kidney injury (AKI) is a life-threatening condition with high morbidity and mortality, characterized by inflammation linked to organelle stress. Despite its clinical significance, effective therapies remain limited. While organelle dysfunction is recognized as a driver of inflammation in AKI, the role of inter-organelle communication in this process remains poorly understood.

Pericoronary adipose tissue radiomics to improve risk stratification for patients with acute coronary syndrome: a multicenter retrospective cohort study.

Background: Pericoronary adipose tissue (PCAT) radiomics derived from coronary computed tomography angiography (CCTA) for predicting major adverse cardiovascular events (MACE) in patients with acute coronary syndrome (ACS) remains unclear. This study aimed to assess whether PCAT radiomics could further provide complementary predictive value for the risk of MACE during long-term follow-up.

Methods: A multicenter retrospective study enrolled 777 subjects who underwent pre-intervention CCTA at 3 medical centers.

Spontaneous vs. Strategic Guilt: Guilt Communication in Repairing Trust with Different Severities of Violations.

Previous research has predominantly focused on the impact of emotions on trust repair, yet has largely neglected how the manner of emotional communication influences this process. Centering on guilt-the emotion most commonly experienced by transgressors following a trust breach-this study examines whether spontaneous versus strategic guilt communication exerts differential effects on trust repair, and explores how the severity of the trust violation moderates this relationship. Study 1 compared the trust repair efficacy of spontaneous and strategic guilt communication.

Stress-stress correlations in two-dimensional amorphous and crystalline solids.

Stress-stress correlations in crystalline solids with long-range order can be straightforwardly derived using elasticity theory (ET). In contrast, the emergent elasticity of amorphous solids, rigid materials characterized by an underlying disordered structure, defies direct explanation within traditional theoretical frameworks. To address this challenge, tensor gauge theories have been recently proposed as a promising approach to describe the emergent elasticity of disordered solids and predict their stress-stress correlations.

Unveiling the impact of bisphenol a exposure on gene expression and immune response in diabetic nephropathy through integrative toxicogenomics and molecular dynamics approaches.

Background: Bisphenol A (BPA) exposure has been implicated in various health issues, including diabetic nephropathy (DN). Understanding the molecular mechanisms underlying BPA-induced DN is crucial for developing effective therapeutic strategies.

Methods: We analyzed the dataset GSE96804 to identify differentially expressed genes (DEGs) associated with DN.

[Expression of Concern] Alterations in left ventricular function during intermittent hypoxia: Possible involvement of O-GlcNAc protein and MAPK signaling.

Following the publication of this paper, for the histopathological data shown in Fig. 2A, it was drawn to the Editor's attention by a concerned reader that, in the 'OGT' row of data panels, the 'Week  0' and 'Week 1' panels appeared to show an overlapping section of data, such that data which were intended to have shown the results of differently performed experiments appeared to have been derived from the same original source. Moreover, the OGT western blots shown in Fig.

The association between serum pinenes and non-alcoholic fatty liver disease based on the NHANES 2013-2014.

Background: Terpenes are known to affect metabolic status, though their relationship with non-alcoholic fatty liver disease (NAFLD) remains unclear.

Methods: Data from the 2013-2014 National Health and Nutrition Examination Survey (NHANES) were utilized in this cross-sectional study. Serum levels of α-pinene, β-pinene, and limonene were sourced from publicly available data provided by NHANES, determined through gas chromatography-tandem mass spectrometry assays.

AlTPS6 from Is a Crucial Regulatory Target for Root-Knot Nematode Infestation.

Root-knot nematodes (RKNs) are widespread parasites that damage crops by weakening the plant immune system, causing root galls and stunting growth. However, the molecular mechanisms behind how RKNs regulate host plant defenses remain largely unknown. The medicinal plant frequently suffers losses from RKNs.

Gut microbiota as a predictive tool for outcomes in IgA nephropathy.

Immunoglobulin A nephropathy (IgAN) is characterized by the deposition of glycosylation-deficient IgA1 in the glomeruli and has been linked to the gut-kidney axis. This study aimed to determine if baseline differences in gut microbiota could predict therapeutic responses in IgAN patients. We analyzed fecal microbiomes of 55 biopsy-confirmed IgAN patients and followed them for over 6 months.

Imaging-Based High-Content Screening with Clickable Probes Identifies XPB Inhibitors.

High-content screening (HCS) has become a powerful tool in drug discovery; however, its reliance on indirect readouts and surrogate markers limits HCS's ability to directly assess drug-protein interactions at endogenous levels, particularly in subcellular contexts. Here, we report an approach to address these limitations by combining confocal imaging-based HCS and bio-orthogonal labeling with clickable probes. As a proof-of-concept, we synthesized a probe Triptolide-alkyne (TL-alk) that rapidly and specifically labels xeroderma pigmentosum type B (XPB), a critical protein in nucleotide excision repair (NER).

A Genetic Perspective to Reveal the Impact of Mitochondrial Dysfunction-related Genes on Diabetic Kidney Disease: A Multi-omics Study.

Objective: This study investigated the causes of Mitochondrial Dysfunction (MD) in Diabetic Kidney Disease (DKD) progression, and identified genes associated with DKD, especially those with significant genetic causal effects, to provide a theoretical basis for DKD treatment.

Methods: Using a large database and single-cell RNA sequencing (scRNA-seq) data, 333 MDRDEGs were discovered. MDRDEGs were linked to AGE-RAGE signaling, RNA processing, protein transport, and energy metabolism using functional enrichment analysis.

Miniaturized Soft and Stretchable Multilayer Circuits through Laser-Defined High Aspect-Ratio Printing.

Stretchable electronics enable seamless integration of wearables with the human body, thereby creating new opportunities in biomedical applications. Miniaturized multilayer stretchable printed circuit boards are key for achieving high functional density circuits with minimal footprint. However, current microfabrication technologies struggle with simultaneously achieving tissue-like softness (<<1 MPa), high resolution and low sheet resistance.

Regulating Adsorption Behaviors in Crystalline Porous Materials by External Electric Fields.

The regulation of adsorption behaviors in crystalline porous materials (CPMs) using external electric fields (E-fields) is an emerging field. CPMs are highly valued for their large surface area, well-ordered pore structures, and chemical versatility, making them ideal for applications in adsorption/separation, catalysis, and biomedicine. In this review, the mechanisms underlying the E-field-induced structural and functional modifications in CPMs, such as phase transitions, framework distortions, and alterations in pore accessibility and selectivity, are delved into.

Sex-specific differences in reproductive tract lymphomas: epidemiology, clinical features, and treatment outcomes.

Background: The differences in the epidemiology, clinical characteristics, and treatment strategies between primary lymphoma of the female reproductive tract (PLFGT) and primary lymphoma of the male reproductive tract (PLMGT) remain unclear.

Materials And Methods: A retrospective analysis of PLFGT and PLMGT data from the surveillance, epidemiology, and end results database spanning 2000 to 2021 was conducted to compare the differences in incidence rates and clinical indicators. Bar chart analysis highlighted the distribution differences between lymphoma subgroups.

Exosome-mediated Induction of Apoptosis in Cisplatin-treated Gastric Cancer Cells as a Strategy to Mitigate Side Effects.

Background: Gastric cancer is the third most lethal malignancy worldwide. While cisplatin has shown remarkable efficacy at a low cost, it is also associated with severe side effects. Exosomes play a key role in mediating the bystander effect of radiation and have the capacity to deliver apoptosis signals for targeted destruction of tumor cell.

Few-shot small vessel segmentation using a detail-preserving network enhanced by discriminator.

Accurate segmentation of small vessels, such as coronary and pulmonary arteries, is crucial for early detection and treatment of vascular diseases. However, challenges persist due to the vessel's small size, complex structures, morphological variations, and limited annotated data. To address these challenges, we propose a detail-preserving network enhanced by a discriminator to improve the few-shot small vessel segmentation performance.

Inhibition of ATG5-mediated autophagy maintains PMAIP1 stability to promote cell apoptosis and suppress triple-negative breast cancer progression.

Objective: Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer characterized by a high recurrence rate and a lack of effective targeted therapies. The purpose of this study was to investigate the interaction between the pro-apoptotic factor phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1) and autophagy-related protein 5 (ATG5), as well as their regulatory mechanisms in TNBC cell apoptosis and autophagy, to identify potential therapeutic targets for TNBC.

Methods: TNBC-related datasets were retrieved from The Cancer Genome Atlas and selected by Prediction Analysis of Microarray 50 analysis to assess the expression of PMAIP1 in the samples.

Zero- to One-Dimensional Transformation in a Highly Porous Metal-Organic Framework to Enhance Physicochemical Properties.

The dynamic behaviors of metal-organic frameworks (MOFs) continue to expand the accessible architectures and properties within this material class. However, the dynamic behaviors that can be studied in MOFs are limited to the transitions, preserving their high crystallinity. For this reason, their significant structural changes involving coordination bond breakage and rearrangement remain largely underexplored.

Control of Mitochondrial Quality: A Promising Target for Diabetic Kidney Disease Treatment.

Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease (ESRD), affecting over 40% of patients with diabetes. DKD progression involves fibrosis and damage to glomerular and tubulointerstitial regions, with mitochondrial dysfunction playing a critical role. Impaired mitochondria lead to reduced adenosine triphosphate (ATP) production, damaged mitochondria accumulation, and increased reactive oxygen species (ROS), contributing to renal deterioration.

Identification of a novel prognostic marker ADGRG6 in pancreatic adenocarcinoma: multi-omics analysis and experimental validation.

Background: Pancreatic adenocarcinoma (PAAD) ranks among the most lethal malignancies worldwide. Current treatment options have limited efficacy, underscoring the need for new therapeutic targets.

Methods: This study employed a multi-omics analytical framework to delve into the expression profiles and prognostic implications of ADGRG6 within the pan-cancer dataset of The Cancer Genome Atlas (TCGA) database, highlighting the prognostic value and potential carcinogenic role of ADGRG6 in PAAD, which was further validated using data from multiple PAAD cohorts in the Gene Expression Omnibus (GEO) database.

Identifying ribonucleotide reductase subunit genes as potential lung adenocarcinomas biomarkers using integrated bioinformatics analysis.

Introduction: Dysregulation of ribonucleotide reductase (RR) subunit genes (RRM1, RRM2 and RRM2B) expression is reported to be involved in the occurrence of various human malignancies. However, the prognostic value of RR subunit genes expression in lung adenocarcinoma (LUAD) patients remains controversial.

Objective: This study aims to analyze the expression profiles, prognostic values, and immune infiltrating associations of RR subunit genes in LUAD to explore whether RR subunit gene expression has value in the prognosis of patients with lung adenocarcinoma (LUAD).

Phosphorylation enhanced OTUD3 deubiquitination ARID3A promotes the progress of cholangiocarcinoma.

Cholangiocarcinoma (CCA) is a highly heterogeneous group of malignant tumors with different molecular etiologies and clinical manifestations. Post-translational modifications (PTM) such as ubiquitination and phosphorylation are widely involved in the progression of CCA. Our aim was to elucidate the effect of the deubiquitinating enzyme OTU domain-containing protein 3 (OTUD3) on the molecular pathogenesis of CAA.

Effects of botulinum toxin type a on nucleotide binding oligomerization domain-like receptor 3 inflammasome in trigeminal ganglion of a rat migraine model.

Background: Botulinum toxin type A (BTX-A) has been used in the prevention and treatment of chronic migraine, but the detailed mechanism was not clear completely.

Objective: The effects of BTX-A on nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome and interleukin-1 beta (IL-1β) were explored in the trigeminal ganglion of migraine model rats.

Methods: Healthy adult male Sprague-Dawley (SD) rats were randomly divided into groups.

Comparing estimated protein excretion rate and spot urinary protein-creatinine ratio in assessing urinary protein excretion in patients with kidney disease in China: a single center study.

Objectives: This study aimed to analyze the potential of the estimated protein excretion rate (ePER) as a substitute for the spot urinary protein-creatinine ratio (uPCR) in clinical reports for accurately assessing urinary protein excretion in China.

Methods: We included 1721 patients in the study and compared the differences in levels, correlation, bias, methodological evaluation between uPCR, ePER, and 24-h urinary protein.

Results: Significant differences ( = -17.