Polystyrene microplastics alter plankton community and enhance greenhouse gas emissions: A case study in the China coastal sea.

Brominated volatile halocarbons (Br-VHCs) emitted from the ocean are the main ozone-depleting substances and greenhouse gases, yet their production dynamics by microorganisms under anthropogenic stressors such as microplastics perturbation are unknown. Here, through coupled ship-based incubations (Yellow Sea) and laboratory experiments, we demonstrate that 1 μm polystyrene (PS) microplastics addition inhibited phytoplankton growth with maximal suppression rates of 82.35% and increased dissolved organic carbon (DOC) accumulation by 91.

A functionally tunable magnetic nanochains platform for N-glycoproteomic analysis of extracellular vesicles from ultratrace biofluids.

Extracellular vesicles (EVs) carry complex glycoproteins critical mediators of disease progression and hold great potential for liquid biopsy applications. However, their low abundance presents significant analytical challenges. This study develop a versatile platform for EV N-glycoproteomic analysis (EVGpro), which enables tandem EV capture, EV protein digestion, and enrichment of N-glycopeptides.

Calculation of DNA damage at different depths of proton SOBP based on a new method and its applications.

In examining the biological effects of proton radiation, DNA is the primary sensitive target. This study utilizes Monte Carlo simulations to efficiently calculate DNA damage yields at various proton depths to analyze the biological effects of protons and their variability on different scales.A new method, the 'Coefficient Method' is used to replace the complete chemical processes by adjusting parameters to obtain suitable values for simulating DNA damage yields at different spread-out Bragg peak (SOBP) depths of low-energy protons, and these parameters are then applied to high-energy proton simulations based on a mesh-type cell model.

A recombinant adenovirus-vectored PEDV vaccine co-expressing S1 and N proteins enhances mucosal immunity and confers protection in piglets.

Porcine epidemic diarrhea virus (PEDV) is a highly contagious enteric coronavirus that causes severe diarrhea and high mortality in neonatal piglets. Effective control requires systemic, mucosal, and T cell-mediated immune responses, highlighting the need for a safe, effective, and versatile vaccine platform. In this study, a recombinant adenovirus type 5-based vaccine co-expressing the PEDV S1 and N proteins (rAd5-S1-N) was developed, and its immunogenicity and protective efficacy were evaluated in mice and piglets.

Influence of selection bias in epidemiology research on extreme temperature and birth outcomes: evidence from a prospective birth cohort in China.

Loss to follow-up can introduce selection bias in birth cohort studies, potentially distorting exposure-outcome associations. Few have assessed the influence of selection bias on the association between prenatal exposure to extreme temperatures and birth outcomes. This study evaluates the influence of selection bias in a Chinese birth cohort.

Carbon monoxide cycling in the marginal sea: A case of the yellow sea and the east China sea.

This study investigated carbon monoxide (CO) dynamics in the Yellow Sea (YS) and the East China Sea (ECS) during spring, revealing significant spatial variability in surface CO concentrations (0.23-3.02 nmol L, mean 1.

Environmental regulation, digital technology application and low-carbon supply chain resilience: Evidence from China.

Enhancing supply chain resilience through market-based environmental policies is crucial for global sustainability amid the escalating climate crisis. This study uses panel data from 887 Chinese A-share listed enterprises between 2008 and 2020 to explore the impact of carbon emissions trading policy on low-carbon supply chain resilience and the moderating role of digital technology application. Grounded in Social Network Theory, low-carbon supply chain resilience is deconstructed into cohesive capacity, restorative capacity, and resistive capacity.

Comprehensive genetic analysis and genotype-phenotype correlations in pediatric patients with atypical hemolytic uremic syndrome.

Background: Atypical hemolytic uremic syndrome (aHUS) is a rare disease caused by the dysregulation of the alternative pathway. The objective of this study was to evaluate the genetic background and genotype-phenotype correlations in pediatric patients with aHUS.

Methods: This retrospective study enrolled 116 pediatric patients from 2013 to 2023 in China.

PTMFusionNet: A Deep Learning Approach for Predicting Disease Related Post-translational Modification and Classifying Disease Subtypes.

With the advancement of technologies such as mass spectrometry, it has become possible to simultaneously perform large-scale detection of protein intensity and corresponding post-translational modification (PTM) information, thereby facilitating clinical diagnosis and treatment. However, existing PTM information is insufficient to fully integrate with protein expression data. We propose a deep learning method called PTMFusionNet, which predicts potential disease-related PTMs and integrates them with protein expression data to classify disease subtypes.

A novel 8-octapeptide repeat insertion in causing Huntington disease-like 1 in a Chinese family: a case report and literature review.

Background: Approximately 1-3% of patients with Huntington disease (HD) present with HD-like phenotype but test negative for the HD gene, suggesting other causes.

Methods: This study presents the first case of Huntington disease-like 1 (HDL-1) in a Chinese family and summarises the clinical features of previously reported HDL-1 cases and patients with octapeptide repeat insertion (OPRI) mutations.

Results: The proband, a 36-year-old woman, presented with progressive involuntary movements, bradykinesia, cognitive decline and personality changes over 6 years, worsening over the past year.

Investigation on the interactions among urea and three major components of lignocellulose in the biomass-based slow-release urea.

It is the theoretical basis to clarify the interaction mechanisms among the three major components of lignocellulose and urea for the utilization of agricultural waste and developing eco-friendly slow-release fertilizers. In this study, individual- and multi-component slow-release urea formulations were prepared from lignocellulose model compounds via the physical blending method. By comparing their physicochemical properties and slow-release performance, it was revealed that the key mechanism underlying the interactions among the three major components and urea is the "supporting skeleton-dense filling-chemical crosslinking" triad.

Porcine respiratory coronavirus in 10 provinces of China: Molecular epidemiology, genetic diversity, and pathogenesis of the isolated PRCV/NM strain.

Respiratory coronaviruses pose significant health threats to both humans and animals. Porcine respiratory coronavirus (PRCV) typically causes mild respiratory infections in pigs and serves as a valuable model for human respiratory coronavirus. However, the investigation of PRCV pathogenesis remains limited.

Shape-controlled hydrogen evolution reaction of single Pd nanocrystals.

This study presents a quantitative investigation of the facet-dependent hydrogen evolution reaction on Pd nanocrystals at the single-particle level, achieved by scanning electrochemical cell microscopy. Notably, Pd octahedra with {111} facets demonstrated significantly superior intrinsic hydrogen evolution reaction activity, as compared to the Pd cubes with {100} facets. This work provides a pedagogical approach for probing the intrinsic activities of nanocrystal electrocatalysts and establishes a definitive structure-activity relationship at the single-entity level.

Prognostic value of the pretreatment Glasgow prognostic score or modified Glasgow prognostic score in patients with advanced cancer receiving immune checkpoint inhibitors: A systematic review and meta‑analysis.

The Glasgow prognostic score (GPS) and modified GPS (mGPS) have value in evaluating the prognosis of patients receiving immune checkpoint inhibitors (ICIs). However, with the continuous emergence of new research, the predictive value of GPS and mGPS for immunotherapy deserves further validation. The aim of the present study was to explore the predictive value of GPS or mGPS on the progression-free survival (PFS) and overall survival (OS) of patients with advanced cancer receiving ICIs.

Effect of organic carbon enrichment on halogenated organic pollutants in wetland sediments of the Yellow River Estuary and Jiaozhou Bay, China.

Coastal wetlands, vital ecosystems, are threatened by anthropogenic activities and act as significant sinks for halogenated organic pollutants (HOPs). Despite the persistent toxicity and ecological risks associated with HOPs, their spatial distribution and environmental behavior in coastal sediments, particularly the role of total organic carbon (TOC) in modulating their fate, remain poorly understood. We investigated the contamination characteristics of 25 halogenated polycyclic aromatic hydrocarbons (HPAHs) in the surface sediments of two coastal wetlands, the Yellow River estuary and Jiaozhou Bay, with different environmental backgrounds.

Koumine mediates the generation of mtROS through the IP3R1-GRP75-VDAC1 complex to improve Citrinin induced intestinal inflammation.

Background: Citrinin (CTN) is a mycotoxin that is difficult to eliminate and easy to ingest. Chronic exposure to CTN can lead to inflammatory bowel disease (IBD). The herb Koumine has strong anti-inflammatory activity and is considered a candidate for the treatment of IBD.

Myeloid deficiency of Z-DNA binding protein 1 restricts septic cardiomyopathy via promoting macrophage polarisation towards the M2-subtype.

Background: Septic cardiomyopathy is a frequent complication in patients with sepsis and is associated with a high mortality rate. Given its clinical significance, understanding the precise underlying mechanism is of great value.

Methods And Results: Our results unveiled that Z-DNA binding protein 1 (ZBP1) is upregulated in myocardial tissues of lipopolysaccharide (LPS)-treated mice.

Protocol for assessing regulatory elements in murine heart using an AAV9-based massively parallel reporter assay.

The mammalian genome contains thousands of regulatory elements whose activities are highly sensitive to external and internal stimulation. Here, we present a protocol to assess the murine cardiac function of regulatory elements using an adeno-associated virus serotype 9 (AAV9)-based massively parallel reporter assay (MPRA). We describe steps for the selection principle of enhancer candidates, MPRA library construction, animal delivery, sequencing library generations, and data analysis pipeline.

Identifying Baicalein as a Key Bioactive Compound in XueBiJing Targeting KEAP1: Implications for Antioxidant Effects.

Background: XueBiJing injection (XBJ) is renowned for its multi-target pharmacological effects, including immunomodulatory, antithrombotic, and antioxidant activities, offering potential therapeutic benefits for patients with severe infections such as sepsis and Coronavirus disease 2019 (COVID-19). Despite its clinical effectiveness, the molecular targets and mechanisms of XBJ remain unclear, warranting further investigation.

Purpose: This study aimed to identify the key bioactive compounds in XBJ and elucidate their molecular targets and mechanisms.

Clinical efficacy of tunnel flap combined with de-epithelialized connective tissue graft in treating gingival recession: a case-series study.

To evaluate the clinical efficacy and influencing factors of the tunnel flap (TUN) combined with de-epithelialized connective tissue graft (DCTG) in the treatment of gingival recession (GR) from patient- and site-specific perspectives. Patients treated with the TUN + DCTG technique for GR between August 2020 and December 2023 were included. Baseline and follow-up periodontal measurements were recorded, including gingival recession depth (GRD), keratinized gingival width (KGW), and clinical attachment loss (CAL).

Investigation of the allelopathic effect of two medicinal plant in agroforestry system.

In order to enhance the sustainable development of the agroforestry system, this work explored the allelopathic effects between two medicinal plants. Siraitia grosvenorii was selected as the recipient plant and investigated the allelopathic effects of different concentrations of fig tree (Ficus carica L.) stem aqueous extracts on seed germination, seedling growth, and physiological indicators of S.

Crystal structure of the possible sulindac impurity 2-(5-fluoro-2-methyl-1-inden-3-yl)aceto-nitrile.

The title compound,CHFN, was identified as a possible critical degradation impurity of sulindac, a therapeutic COX-2 inhibitor for rheumatoid arthritis. Single-crystal X-ray analysis revealed two conformationally slightly different mol-ecules in the asymmetric unit (' = 2), each containing an indene ring system. In the crystal structure, [100] chains formed through C-H⋯N inter-actions are connected into a tri-periodic supra-molecular structure by further C-H⋯F and C-H⋯π inter-actions through 2/ symmetry operations.

Enhancing bloodstream infection diagnostics: a novel filtration and targeted next-generation sequencing approach for precise pathogen identification.

Bloodstream infections (BSIs) pose a significant diagnostic challenge, largely due to the limitations of traditional methods such as blood cultures. These methods often yield low positive rates, have lengthy processing times that delay treatment, and are limited in detecting only a narrow range of pathogens. Such delays and inaccuracies can critically impede timely clinical interventions, potentially compromising patient outcomes.

A new numerical method for calculating residual deformation in mined-out areas considering water-rock interaction and its application.

Globally, extensive land regions have fallen victim to coal mining subsidence, rendering the reuse of goaf sites a crucial concern. The residual deformation amount of these sites is the linchpin for determining their reusability. Presently, numerical computations of residual deformation in goafs, which overlook water-rock coupling, breed significant errors, posing severe threats to the safety of on-site structures.

MethPriorGCN: a deep learning tool for inferring DNA methylation prior knowledge and guiding personalized medicine.

DNA methylation plays a crucial role in human diseases pathogenesis. Substantial experimental evidence from clinical and biological studies has confirmed numerous methylation-disease associations, which provide valuable prior knowledge for advancing precision medicine through biomarker discovery and disease subtyping. To systematically mine reliable methylation prior knowledge from known DNA methylation-disease associations and develop robust computational methods for precision medicine applications, we propose MethPriorGCN.