Multimodal radiomics model with triple -timepoint contrast-enhanced ultrasound for precise diagnosis of C-TIRADS 4 thyroid nodules.

Objective: This study aims to construct a multimodal radiomics model based on contrast-enhanced ultrasound (CEUS) radiomic features, combined with conventional ultrasonography (US) images and clinical data, to evaluate its diagnostic efficacy in differentiating benign and malignant thyroid nodules (TNs) classified as C-TIRADS 4, and to assess the clinical application value of the model.

Methods: This retrospective study enrolled 135 patients with C-TIRADS 4 thyroid nodules who underwent concurrent US and CEUS before FNA/surgery. From each case, one US image and three CEUS key frames (2s post-perfusion, peak enhancement, 2s post-peak) were selected.

Behavior-specific intervention: anti-escape trap net physically control Pimelocerus perforatus (Roelofs, 1873) (Coleoptera: Curculionidae) by exploiting ascending-descending behavior.

Background: The weevil Pimelocerus perforatus (Roelofs, 1873) is a major wood-boring pest of Luteolaceae, causing significant ecological damage and economic loss in China. Currently, there are no effective control methods due to their hidden feeding sites and lack of effective natural predators. The use of chemicals pesticides causes severe pollution.

Harnessing cellular immunity for next-generation vaccines against respiratory viruses: mechanisms, platforms, and optimization strategies.

Respiratory tract infections, such as influenza, respiratory syncytial virus (RSV) infection, and COVID-19, remain a persistent threat to global public health due to their high transmissibility and disease burden. Vaccination, as a key preventive strategy, not only reduces the risk of infection but also blocks transmission by activating adaptive immunity. While traditional vaccine evaluations have primarily focused on humoral immunity, growing evidence highlights the critical role of T lymphocyte-mediated cellular immunity in clearing virus-infected cells, establishing long-term immune memory, and responding to viral mutations.

Maternal blood glucose level is correlated to offspring brain fatty acid composition in a mouse model for gestational diabetes mellitus.

Gestational diabetes mellitus (GDM) may increase the risk of suboptimal neurocognitive development in infants. Maternal supply of omega-3 and -6 long chain polyunsaturated fatty acids (LCPUFAs), including eicosapentaeonic acid, docosahexaenoic acid and arachidonic acid, are critical for offspring brain development. To study the effects of GDM on offspring brain fatty acid composition, C57BL/6J mice were exposed to short-term high-fat diet feeding and low-dose streptozotocin treatments before pregnancy.

The emerging role of miR-22 in inflammation.

MicroRNA-22 (miR-22), discovered in 2007, is a small non-coding RNA comprising 22 nucleotides. It is widely expressed in organisms and conserved across different species. A series of studies have shown that miR-22 not only regulates the growth and differentiation of various cells and tissues but also participates in the occurrence and development of many diseases.

Damaging effect of ischemia on the development of retinal organoids derived from human embryonic stem cells.

Aim: To explore the changes in early retinal development after the occurrence of ischemia.

Methods: Human retinal organoids (hROs) of day 18 or day 30 were treated with oxygen-glucose deprivation and reperfusion (OGD/R) to simulate the retinal ischemia. All hROs were maintained normally until day 60 to evaluate changes in ischemic injuries during retinal development.

Ultrasound-driven modulation of myofibrillar protein conformation promotes salt uptake and taste enhancement in marinated chicken breast.

Enhancing salt diffusion and flavour development in dense muscle matrices remains a key challenge in poultry marination. This study aimed to explore how ultrasound-assisted marination modulates protein structure and improves salt uptake and flavour development in chicken breast meat. Ultrasound treatment (150-750 W) altered muscle microstructure by disrupting myofibrillar alignment, loosening fibres, and modifying surface properties.

Endoscopic Release of the Carpal Ligament: A Sheathless Tube Technique for Carpal Tunnel Syndrome.

Minimally invasive techniques have become the standard of care for multiple procedures. Our sheathless tube technique makes minimally invasive techniques for carpal tunnel syndrome simpler and offers reduced incision pain, improved aesthetic outcomes, and shorter recovery time. The technique involves using the palmar longus tendon to create the operative space while using it as a guide to release the transverse carpal ligament and achieve nerve decompression.

Using Fibularis Tertius Tendon for V-Shaped Double-Bundle Anatomic Anterior Talofibular Ligament Reconstruction.

Multiple autografts have been employed in reconstructing anterior talofibular ligament (ATFL) in patients with chronic lateral ankle instability. However, conventional techniques using peroneal tendons longus/brevis as graft sources carry inherent risks of donor site morbidity. This Technical Note describes using the fibularis tertius tendon for V-shaped double-bundle anatomic ATFL reconstruction in patients with chronic lateral ankle instability.

High-resolution and large-stretch single photon-gated sampling of chaotic laser.

Achieving high-fidelity acquisition and reconstruction of chaotic signals at a single-photon level remains a key challenge in secure communication and ultrasensitive sensing, primarily due to bandwidth and resolution limits of single-photon detectors (SPDs). In this work, we present an experimental method to recover high-bandwidth chaotic signals using low-bandwidth and high-sensitivity SPDs, which effectively mitigates the impacts of detector dead time and time jitter by shifting the signal spectrum into the SPD's response window and constructing a programmable gated sampling to time-stretch the signal. It achieves a maximum time stretching multiplication of 122 times, and the temporal resolution of SPD is improved by more than 24 times.

Remote ischaemic conditioning improves outcomes of ischaemic stroke treated by endovascular thrombectomy: the SERIC-EVT trial.

Background And Aims: Even after endovascular thrombectomy, more than half of patients with acute large vessel occlusion stroke do not achieve favourable outcomes. This study aimed to assess the efficacy and safety of remote ischaemic conditioning (RIC), a promising neuroprotective treatment, in patients with acute ischaemic stroke who received endovascular thrombectomy.

Methods: This participant-blinded, randomized controlled clinical trial was conducted at 25 hospitals.

Redefining tear film Biology: Immune regulation, multi-omics integration, and systemic disease interfaces.

Traditionally considered a passive lubricant, the tear film, comprising three functionally distinct yet interdependent layers (lipid, aqueous, mucin), is now recognized as a dynamic interface that integrates ocular and systemic immune regulation. It orchestrates innate, adaptive, and tolerogenic immune responses to preserve ocular surface homeostasis. In dry eye disease (DED), dysfunction of these regulatory pathways initiates inflammatory cascades, leading to goblet cell loss and epithelial injury.

Factors Affecting the MicroRNA Expression Pattern in the Chorioamniotic Membrane: From the Perspective of Preterm Labor.

Preterm labor is a major contributor to neonatal mortality and morbidity worldwide. Despite extensive research, the molecular mechanisms driving preterm labor are not fully understood. Epigenetic modifications may play a role in the underlying mechanisms leading to adverse pregnancy outcomes, particularly preterm labor.

Hypoxia combined with a high-fat diet aggravates pulmonary vascular endothelial dysfunction in rats.

Objectives: Hypoxia and high-fat diet are independent risk factors for pulmonary vascular endothelial dysfunction (VED); however, their interactive effects are poorly understood. This study aimed to elucidate the effect and mechanism of hypoxia combined with a high-fat diet on the pulmonary arterial endothelial structure and function in rats.

Methods: Forty rats were randomly assigned to a normal diet and normoxia, a normal diet and hypoxia, a high-fat diet and normoxia, and a high-fat diet and hypoxia.

Mechanism of San Jie Tong Mai Fang in Atherosclerosis Attenuation by JAK/STAT-mediated Inhibition of Macrophage M1 Polarization.

Macrophage polarization influences the atherosclerotic plaque microenvironment by driving inflammatory responses, with the JAK/STAT signaling pathway serving as a critical regulator of this process. This study investigated whether San Jie Tong Mai Fang (SJTMF), an herbal formulation reported to promote M2-type macrophage polarization, alleviates atherosclerosis (AS) by modulating the JAK/STAT signaling pathway. An AS model was established in ApoE mice via 12-week high-fat diet feeding, followed by 4-week treatment with SJTMF alone or combined with a JAK inhibitor or macrophage scavenger.

Water-soluble ratiometric fluorescent probe for dual-channel and naked-eye detection of heavy water.

Heavy water (DO) is critical in diverse scientific and industrial applications, yet its discrimination from ordinary water (HO) remains challenging due to their similar physicochemical properties. Herein, we present a series of D-π-A type small-molecule probes-HTCPP-H, HTCPP-F, HTCPP-Cl, and the water-soluble HTCPP-F-PEG-designed via precise ground-state proton transfer mechanisms for dual-mode ratiometric fluorescence and colorimetric detection of DO in aqueous media. These probes afford distinct simultaneous changes in both color and fluorescence upon exposure to DO, enabling rapid, naked-eye discrimination and accurate quantification without requiring organic cosolvents.

A prognostic score based on early recurrence-related factors for hepatocellular carcinoma patients with microvascular invasion after hepatectomy.

Purpose: We aimed to construct a simple risk score to stratify individuals with distinguished prognosis for HCC patients with microvascular invasion (MVI).

Methods: 265 qualified patients were retrospectively analyzed. A prognostic score was constructed using predictors associated with early recurrence (ER).

The environmental adaptation of acidophilic archaea: promotion of horizontal gene transfer by genomic islands.

Acid mine drainage (AMD) is an extremely acidic leachate highly contaminated with metal ions, yet it harbors a significantly high abundance of archaea. Genomic islands (GIs), as one of the productions of horizontal gene transfer (HGT), play an important role in the environmental adaptation and evolutionary processes of archaea. However, the distribution, structure, and function of GI within the genomes of archaea remain poorly understood.

Effects of traditional Chinese exercises on cardiopulmonary function in stroke patients: A meta-analysis.

Background: Traditional Chinese exercises may be effective in improving cardiorespiratory fitness in post-stroke patients; however, no study has conducted a meta-analysis to assess their its efficacy in meta.

Objective: The purpose of this meta-analysis is to evaluate how traditional Chinese exercises affect post-stroke cardiorespiratory fitness.

Methods: We conducted a comprehensive search across eight Chinese and English databases, including Wan Fang, VIP, CBM, PubMed, Embase, Web of Science, and the Cochrane Library, from the inception of these databases until May 2025.

Toxicological Effects of Glufosinate-Ammonium-Containing Commercial Formulations on in Aquatic Environments: A Multidimensional Study from Embryotoxicity to Histopathology.

Glufosinate-ammonium (GLA) is a broad-spectrum herbicide widely used for weed control. However, its potential toxic effects on non-target aquatic organisms, especially in freshwater ecosystems, are of growing concern. This study investigates the toxic effects of GLA on , a freshwater snail highly sensitive to environmental pollutants and commonly used as a model organism in toxicological studies.

Toxicological effects of dimethyl fumarate in Biomphalaria glabrata: A multi-stage study from embryo to adult.

Dimethyl Fumarate (DMF) is a widely used organic synthetic intermediate known for its low toxicity, high efficiency, and broad-spectrum antimicrobial properties. It is commonly applied in food preservation and medical treatments. However, with the increasing use of DMF, its toxic effects on living organisms have attracted more and more attention.

Progressive gut microbiota shifts and functional alterations across aging stages and frailty in mice.

The gut microbiota modulates aging through metabolic and immune interactions. To characterize microbial changes during aging, we profiled gut microbiota across four stages in male C57BL/6J mice: young-adult, middle-aged, senescent, and frailty. Using 16S rRNA sequencing and targeted metabolomics, we observed a biphasic diversity pattern, with reduced richness in senescence followed by a rebound in frailty.

Comparing AI-generated and traditional textbook multiple-choice questions in nursing education: A prompt engineering-based Delphi study.

Objective: To explore the potential and challenges of GLM-4 model in nursing test design, and to evaluate its effectiveness in generating multiple-choice questions under the theme of management of surgical shock patients.

Methods: The Delphi method was employed, inviting 8 nursing education experts with master's degrees or higher, or associate professor titles and above, to review 25 MCQs generated by GLM-4. Various prompting techniques (e.

Chronic inflammation in Long COVID relationship to autoimmune diseases.

The new coronavirus pandemic has been ongoing for nearly five years. In addition to the severe symptoms in the acute phase, it is accompanied by long-term complications and sequelae involving the respiratory, neurological, immune, circulatory, and gastrointestinal systems for several months or even years, which is called the Long COVID. Many studies have suggested that systemic chronic inflammation caused by residual viral components may be one of the pathophysiologic mechanisms of Long COVID.

Amoxicillin-loaded chitosan coated magnetic nanocarriers as a dual-platform for anaerobic bacterial preconcentration and gene-sensing in periodontal pathogen detection.

Periodontitis, a progressive inflammatory condition, causes periodontal tissue destruction, pain, chewing difficulties, and tooth loss, primarily due to pathogenic oral biofilms dominated by low abundance periodontopathic bacteria. Accurate diagnosis is hindered by the limited bacterial presence in saliva, necessitating effective enrichment techniques. This study introduces novel amoxicillin-loaded chitosan-coated magnetic nanocarriers (AMX-CS-MNCs) designed for rapid capture, enrichment, and DNA extraction of Gram-positive and Gram-negative anaerobic periodontal pathogens, including Porphyromonas gingivalis and Parvimonas micra, from artificial and clinical saliva samples collected at Kangmei Dental Hospital, facilitating highly sensitive PCR-based detection.