Metabolome analysis and functional characterization reveals that d-gluconic acid contributes to pine defence against Bursaphelenchus xylophilus infection.

Background: Pines (Pinus L.) are widely cultivated for their rich resin content and ease of wood processing. However, pines are constantly threatened by pine wood nematode (PWN; Bursaphelenchus xylophilus), causing a large number of pine forests to wilt and die.

Hypoxia combined with a high-fat diet aggravates pulmonary vascular endothelial dysfunction in rats.

Objectives: Hypoxia and high-fat diet are independent risk factors for pulmonary vascular endothelial dysfunction (VED); however, their interactive effects are poorly understood. This study aimed to elucidate the effect and mechanism of hypoxia combined with a high-fat diet on the pulmonary arterial endothelial structure and function in rats.

Methods: Forty rats were randomly assigned to a normal diet and normoxia, a normal diet and hypoxia, a high-fat diet and normoxia, and a high-fat diet and hypoxia.

Systematic Investigation of Phosphate Decomposition and Soil Fertility Modulation by the Filamentous Fungus .

Phosphate-solubilizing microbes (PSMs) in soil play a crucial role in converting insoluble phosphates into plant-available soluble phosphorus. This paper systematically presents a comprehensive array of qualitative and quantitative techniques to assess the phosphate-decomposing capabilities of microbes. Additionally, it introduces two optimized media, namely improved Monkina medium No.

Expression and Antagonistic Activity Against Plant Pathogens of the Phage Tail-like Protein from WS-FJ9.

Microorganisms exert antagonistic effects on pathogens through different mechanisms, thereby achieving biological control of plant diseases. Many strains can produce complex secondary metabolites and substances that have toxic effects on host cells. The phage tail-like bacteriocins (tailocins) is a compound with antibacterial activity.

Risk Estimation of Carcinogenic and Noncarcinogenic Diseases from Radiation for Medical X-ray Workers.

The objective of this paper is to construct a follow-up cohort of medical x-ray workers and analyze the risk estimates of radiation-induced carcinogenic and noncarcinogenic diseases induced by chronic low-dose ionizing radiation exposure in the follow-up cohort. A fixed cohort study was used. A total of 159 medical x-ray workers working in radiology departments of hospitals in Gansu Province from 1950 to 1980 were selected as the radiology group, and 149 medical workers in internal medicine, surgery, and other departments who had not engaged in radiology work at the same hospital were selected as the control group.

Biocontrol activity and action mechanism of Pseudomonas aurantiaca ST-TJ4 against Verticillium dahliae, the causal agent of Acer truncatum wilt.

Acer truncatum wilt caused by Verticillium dahliae is a severe soilborne disease that poses a threat to the cultivation of this plant in China. The present study explored the biocontrol efficiency and underlying antagonistic mechanism of Pseudomonas aurantiaca ST-TJ4 against V. dahliae.

Treatment of pediatric refractory or relapsed Epstein-Barr virus-associated hemophagocytic syndrome with PD-1 inhibitors.

Purpose: Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH) is a type of pediatric HLH that occurs frequently in Asia. Although immunochemotherapy based on etoposide and hormone has improved survival rates, there are still about 30% of HLH patients that do not respond. The objective of the article is to examine the efficacy and safety of programmed cell death protein 1 (PD-1) inhibitors for children with relapsed/refractory (r/r) EBV-HLH.

Volatiles emitted by Pseudomonas aurantiaca ST-TJ4 trigger systemic plant resistance to Verticillium dahliae.

Verticillium dahliae is among the most devastating fungal pathogens, causing significant economic harm to agriculture and forestry. To address this problem, researchers have focused on eliciting systemic resistance in host plants through utilizing volatile organic compounds (VOCs) produced by biological control agents. Herein, we meticulously measured the quantity of V.

A pioneer nematode effector suppresses plant reactive oxygen species burst by interacting with the class III peroxidase.

Bursaphelenchus xylophilus is the pathogen of pine wilt disease, which can devastate the pine forest ecosystem. Usually, plant cells generate reactive oxygen species (ROS) as a defensive substance or signalling molecules to resist the infection of nematodes. However, little is known about how B.

The impact of virtual clinical simulation on nursing students' palliative care knowledge, ability, and attitudes: A mixed methods study.

Background: Palliative care is fraught with numerous challenges when it comes to conducting practical teaching as it involves caring for people facing the complexities of end-of-life and death. Insufficient clinical practice hinders nursing students from mastering knowledge, attitude and ability of hospice care. Virtual clinical simulation has demonstrated its effectiveness as a valuable educational tool in nursing.

The Bursaphelenchus xylophilus candidate effector BxLip-3 targets the class I chitinases to suppress immunity in pine.

Lipase is involved in lipid hydrolysis, which is related to nematodes' energy reserves and stress resistance. However, the role of lipases in Bursaphelenchus xylophilus, a notorious plant-parasitic nematode responsible for severe damage to pine forest ecosystems, remains largely obscure. Here, we characterized a class III lipase as a candidate effector and named it BxLip-3.

The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds.

Persistence in the soil is a function of seed physiology, particularly non-germination and inherent lifespan. However, for seeds with mechanical dormancy, non-germination is also a function of the composition and activity of the soil microbiota. We attempted to screen out microorganisms in the soil that can specifically and rapidly decompose the hard fruit pericarps of Maxim.

Correction: Aromatic amine electrochemical sensors based on a Co-MOF: a hydrogen bond-induced specific response.

Correction for 'Aromatic amine electrochemical sensors based on a Co-MOF: a hydrogen bond-induced specific response' by Xiao-qin Wu , , 2022, , 16861-16869, https://doi.org/10.1039/d2dt02049a.

Transcriptomic analysis reveals differentially expressed genes associated with pine wood nematode resistance in resistant Pinus thunbergii.

Pine wilt disease (caused by the nematode Bursaphelenchus xylophilus) is extremely harmful to pine forests in East Asia. As a low-resistance pine species, Pinus thunbergii is more vulnerable to pine wood nematode (PWN) than Pinus densiflora and Pinus massoniana. Field inoculation experiments were conducted on PWN-resistant and -susceptible P.

Dual inoculation with rhizosphere-promoting bacterium Bacillus cereus and beneficial fungus Peniophora cinerea improves salt stress tolerance and productivity in willow.

Utilization of rhizosphere microorganisms to improve plant growth and salt tolerance has recently attracted widespread attention. The growth and salt tolerance of willows inoculated with Bacillus cereus JYZ-SD2 and Peniophora cinerea XC were studied under different salt stress conditions. The results showed that the chlorophyll content of willow cuttings inoculated with the XC strain increased significantly by 51.

Thyroid dose assessments due to inhalation of I for nuclear medicine workers.

Background: In general, medical staff who work in nuclear medicine should be entirely safe in their professional environment. Nevertheless, we already know that the working environment of the nuclear medicine staff is not completely safe due to the handling of high amounts of radionuclides for diagnostic and therapy applications, which is especially relevant for I (as a non-sealed source).

Purpose: The goal of this study was to assess the inhaled I thyroid dose in nuclear medicine workers and to introduce a simple method for internal exposure monitoring.

Aromatic amine electrochemical sensors based on a Co-MOF: a hydrogen bond-induced specific response.

A 2D Co-MOF, {[Co(L)(bipy)](DMA)·2HO} (Co-1, HL = 2,5-thienedioic acid; bipy = 2,2'-bipyridine; DMA = ,'-dimethyl acetamide), was synthesized by hydrothermal method. Co-1 has excellent air stability. When modifying the surface of a glassy carbon electrode (GCE) with Co-1, the obtained electrochemical senor Co-1/GCE shows excellent sensitivity towards 1,3-dinitrobenzene (-DNB) and 2,4-dinitroaniline (2,4-DNA), although the electrochemical conductivity of Co-1 is not that good.

JZ-GX1 alleviates iron deficiency chlorosis in by secreting desferrioxamine and reshaping the soil fungal community.

Plant growth-promoting rhizobacteria are important for improving plant iron nutrition, but the interactions among inoculants, host plants and soil microorganisms have not been greatly explored. JZ-GX1 was applied to treat the increasingly serious iron deficiency chlorosis in , and the resulting improvement in chlorosis was determined by assessing the contents of chlorophyll, active iron, Fe and antioxidant enzymes in leaves, the effects on the soil microbial community and the metabolism in the rhizosphere by high-throughput sequencing techniques and liquid chromatography-mass spectrometry (LC-MS). The results showed that inoculation with JZ-GX1 significantly increased the chlorophyll content of , which promoted the redistribution of active iron in roots and leaves, increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), and thus reduced membrane damage in iron-deficient caused by reactive oxygen species.

The key molecular pattern BxCDP1 of induces plant immunity and enhances plant defense response two small peptide regions.

The migratory plant-parasitic nematode is the pathogen of the pine wilt disease (PWD), causing serious damage to pine forests in China. During the process of plant resistance to multiple pathogens, plant immunity plays a key role. In this current study, the pathogen-associated molecular pattern (PAMP) BxCDP1 in has been identified, but the host target protein of BxCDP1 and its key amino acid region inducing the plant immunity have yet to be elucidated.

Effects of Volatile Organic Compounds Produced by ST-TJ4 against .

is one of the most destructive fungal pathogens, causing substantial economic losses in agriculture and forestry. The use of plant growth-promoting rhizobacteria (PGPR) is an effective and environmentally friendly strategy for controlling diseases caused by . In this study, 90 mm in diameter Petri plates were used to test the effect of volatile organic compounds (VOCs) produced by different concentrations of ST-TJ4 cells suspension on mycelia radial growth and biomass.