Inhibition of CPT1A activates the cGAS/STING pathway to enhance neutrophil-mediated tumor abrogation in triple-negative breast cancer.

Triple-negative breast cancer (TNBC) remains a challenging malignancy to treat, underscoring the urgent need to explore novel and effective therapeutic targets. In this study, we found that carnitine palmitoyltransferase 1A (CPT1A), the central and rate-limiting enzyme for fatty acid oxidation (FAO) in lipid metabolism, is significantly correlates with poor survival outcomes in TNBC patients and is highly expressed in TNBC patient samples. Inhibition of CPT1A greatly suppresses TNBC tumor growth.

Cutaneous infection caused by Mycobacterium boenickei due to lipolysis injection: A case report.

Cosmetic injection has emerged as a significant cause of cutaneous infections associated with rapidly growing mycobacteria (RGM). Mycobacterium boenickei, a rare RGM first isolated from a leg wound in the United States in 2004, has been reported in only six strains worldwide. However, clinical cases of M.

Triple-synergistic hollow AuAg@CeO plasmonic nanozymes enable rapid alkaline phosphatase detection via smartphone-integrated dual-mode biosensing.

Alkaline phosphatase (ALP), a pivotal hydrolase in human tissues, serves as a key biomarker for disease diagnostics. Here, we report a hollow AuAg@CeO plasmonic nanozyme engineered for ALP detection through triple synergistic catalysis. The hierarchically designed hollow architecture enhances near-infrared (NIR) photon capture via multiscale light scattering, while the AuAg-CeO heterojunction enables directional charge transfer.

Programmed Design of Nitric Oxide and Photodynamic Therapy Based on Orthogonal Upconversion Nanoparticles Improves Anti-Tumor Efficacy via Protein S-nitrosylation.

The effectiveness of cancer treatment has been significantly improved through combined therapies that synergistically utilize multiple treatment modalities. While combination strategy mitigates tumor resistance, enhances therapeutic efficacy, and reduces side effects compared to monotherapies, the precise coordination of treatment sequences and timing remains underexplored, particularly in the nanomaterial-based therapies. In this study, this gap is addressed by investigating the interaction between photodynamic therapy (PDT) and nitric oxide (NO) therapy, with a specific focus on the sequence in which these therapies are administered.

Rapid and sensitive detection of using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts.

Unlabelled: Tuberculosis remains a major global health threat, with existing detection methods often limited by efficiency and resource demands. Our previous PAM-dependent dsDNA Target-activated Cas12f1 Reporter (PDTCTR) fluorescence sensing platform, while effective for PAM-dependent pathogen detection, was constrained by its reliance on specialized fluorescence equipment and lack of visual output, limiting its use in resource-limited settings. To overcome these limitations, we introduce an innovative RPA/CRISPR-Cas12f1_ge4.

Translation regulation in Bacillus subtilis and its applications in heterologous protein expression: A review.

Bacillus subtilis is widely used for industrial enzyme production due to its food safety and good capability of protein synthesis and secretion. However, the production of heterologous proteins is often inefficient, partly due to poor compatibility and versatility of genetic elements in B. subtilis.

Grading magnetic resonance imaging signs for diagnosing invasive placenta accreta spectrum disorders.

Introduction: Placenta accreta spectrum (PAS) disorders result from abnormal placental attachment, leading to varying degrees of myometrial invasion. Magnetic resonance imaging (MRI) plays a crucial role in assessing the depth and extent of placental invasion. This study aims to evaluate the correlation between quantified MRI findings and the diagnosis of PAS, as classified according to the FIGO system.

Langhans giant cells regulate cutaneous immune responses during mycobacterial infection through CXCL1/CXCL2 secretion.

Mycobacterial infection poses a significant threat to human health, with granuloma formation serving as a hallmark pathological feature and a critical diagnostic indicator. Langhans giant cells (LGCs) play a pivotal role in the immune response during mycobacterial infections, yet their immunological functions remain incompletely understood. In this study, we explored the role of LGCs in immune modulation during mycobacterial infection.

Analysis of the efficacy of Langhans giant cells in controlling cutaneous Mycobacterium marinum infections.

Background: Cutaneous Mycobacterium marinum (M. marinum) infection can lead to the formation of infectious granulomas containing Langhans giant cells (LGCs). Due to concerns about prolonged antibiotic use and the development of drug resistance, its treatment poses challenges.

[Protective effect of K12 against infection in mice].

Objectives: To investigate the protective effect of the probiotic bacterium K12 (K12) against (Mp) infection in mice.

Methods: Forty male BALB/c mice were randomized into normal control group, K12 treatment group, Mp infection group, and K12 pretreatment prior to Mp infection group. The probiotic K12 was administered daily by gavage for 14 days before Mp infection induced by intranasal instillation of Mp.

Modification of Glucose Metabolic Pathway to Enhance Polyhydroxyalkanoate Synthesis in .

Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are semi-crystalline elastomers with a low melting point and high elongation at break, allowing for a wide range of applications in domestic, agricultural, industrial, and mainly medical fields. Utilizing low-cost cellulose hydrolyzed sugar as a carbon source and metabolic engineering to enhance synthesis in is a promising strategy for commercializing mcl-PHAs, but little has been attempted to improve the utilization of glucose for synthesizing mcl-PHAs. In this study, a multi-pathway modification was performed to improve the utilization of substrate glucose and the synthesis capacity of PHAs.

[Bioinformatics, expression, purification, and inflammation-inducing effect of GroEL protein].

To preliminarily understand the pathogenic mechanism of (Mg) GroEL protein, we used bioinformatics tools to predict the structure and function of Mg GroEL protein and then constructed the recombinant plasmid pET-28a-GroEL. The protein expression was induced by 0.2 mmol/L IPTG, and the expressed protein was purified by Ni-iminodicitic acid (IDA) column affinity.

The effect of digital government on corporate total factor productivity.

This study examines the influence of digital government initiatives on corporate total factor productivity (TFP). Employing a difference-in-differences (DID) methodology and analyzing data from publicly listed companies spanning the period 2010 to 2020, we investigate the impact of digital governance on corporate TFP. Our findings reveal a noteworthy positive effect, with an average TFP increase of 5%.

Unraveling the obesity paradox in small cell lung cancer immunotherapy: unveiling prognostic insights through body composition analysis.

Background: The advent of immunotherapy has changed the landscape of SCLC treatment, although the identification of reliable prognostic biomarkers remains a formidable challenge. Our objective was to investigate the prognostic implications of obesity and body composition in SCLC immunotherapy while seeking a straightforward anthropometric measure.

Methods: This retrospective study analyzed data from patients with SCLC who underwent immunotherapy between 2019 and 2023.

ERA-CRISPR/Cas12a system: a rapid, highly sensitive and specific assay for .

Introduction: , the causative agent of human tuberculosis, poses a significant threat to global public health and imposes a considerable burden on the economy. However, existing laboratory diagnostic methods for are time-consuming and have limited sensitivity levels.

Methods: The CRISPR/Cas system, commonly known as the "gene scissors", demonstrates remarkable specificity and efficient signal amplification capabilities.

Magnetic resonance imaging of abnormal placental shapes with correlation with pathologic findings.

Objective: This study aimed to investigate the magnetic resonance imaging (MRI) characteristics of abnormal placental shapes (APS) compared with prenatal ultrasound.

Methods: From an initial cohort of 613 women with a high prevalence of placenta accreta spectrum (PAS) disorders, the MRI findings of 27 pregnant women with APS who underwent antenatal ultrasound and MRI examinations before delivery were retrospectively analyzed. The clinicopathological findings were used as the gold standard, and the sensitivity, specificity, and accuracy of antenatal MRI and a multidisciplinary team experienced in diagnosing APS were assessed.

Magnetic resonance imaging diagnosis of abnormal placental cord insertions.

Introduction: Abnormal placental cord insertions (APCIs) are significant risk factors for pregnancy complications, encompassing marginal cord insertion (MCI), velamentous cord insertion (VCI), and vasa previa (VP). While ultrasound is the primary imaging modality, its accuracy can be limited by factors such as maternal obesity and fetal positioning. Complementary to ultrasound, magnetic resonance imaging (MRI) offers a more precise visualization of the fetus, placenta, and umbilical cord relationships.

Preparation of W-N-C single atom catalyst and Cu(HHTP) metal-organic framework dual-decorated graphene nanoplatelet flexible electrode arrays for the rapid detection of carbendazim in vegetables.

It is highly desirable to develop a low-cost and rapid detection method for trace levels of carbendazim fungicide residues, which would be beneficial for improving human health and mitigating environmental issues. Herein, isolated single tungsten atoms were implanted onto well-organized metal-organic framework (MOF)-derived N-doped carbons to form W-N-C single-site heterojunctions with ultrahigh electrocatalytic activity. The coupling of W-N-C with Cu(HHTP), an electronically conductive MOF with a large surface area and porous structure, exhibited enhanced electrocatalytic performance for the oxidation of carbendazim (CBZ) when they were used for decorating graphene nanoplatelet flexible electrode arrays fabricated via template-assisted scalable filtration.

Clinical effect of spleen aminopeptide on improving liver function damage and immune function in children with infant hepatitis syndrome.

Background: Infant hepatitis syndrome (IHS) is a clinical syndrome in infants less than one year of age with generalized skin jaundice, abnormal liver function, and hepatomegaly due to various etiologies such as infection.

Aim: To investigate the effect of IHS patients, after treatment with arsphenamine-based peptides, on patients' liver function damage and immune function.

Methods: Of 110 patients with IHS treated in our hospital from January 2019 to January 2021 were grouped according to the randomized residual grouping method, with 5 cases in each group shed due to transfer, Ultimately, 50 cases remained in each group.

Regulatory Mechanism of Proanthocyanidins in Grape Peels Using vvi-miR828a and Its Target Gene .

Anthocyanins and proanthocyanidins are considered to be essential secondary metabolites in grapes and are used to regulate metabolic processes, while miRNAs are involved in their synthesis of anthocyanins and proanthocyanidins to regulate metabolic processes. The present research work was carried out to investigate the underlying regulatory mechanism of target genes in the grape cultivars 'Italia' and 'Benitaka'. miRNA and transnscriptomic sequencing technology were employed to characterize both the profiles of miRNAs and the transcripts of grape peels at 10 and 11 weeks post flowering (10 wpf and 11 wpf).