Integrated Analysis of Single-cell and Bulk RNA-Sequencing Identifies a Signature Based on Cancer-related Fibroblast Marker Genes to Predict Prognosis and Therapy Response in Lung Adenocarcinoma.

Cancer-related fibroblasts (CAFs), crucial in the tumor microenvironment, significantly influence tumorigenesis and extracellular matrix shaping. This study aimed to analyze the expression of CAF marker genes in lung adenocarcinoma (LUAD) and create a prognostic signature. We included 716 LUAD patients from different cohorts, conducting a comprehensive analysis of single-cell RNA sequencing data from the Gene Expression Omnibus (GEO) database, identifying 227 CAF marker genes.

Security-enhanced ultra-high-order QAM quantum noise stream cipher assisted by intensity and phase chaotic encryption.

An ultra-high-order QAM quantum noise stream cipher (QNSC) scheme assisted by intensity and phase chaotic encryption is proposed. In this scheme, the legal transmitter (Alice) first encrypts the original low-order QAM signal into an ultra-high-order QAM signal according to the pre-shared seed key based on the Y-00 protocol, and then conceals the intensity and phase information of the ultra-high-order QAM signal through the intensity and phase chaos generated from a nonlinear delay chaotic source for enhancing the security. The intensity and phase chaotic carrier can be eliminated by the open-loop chaotic synchronization module at the legal receiver (Bob).

Branched-Chain α Keto-Acid Dehydrogenase Kinase-Mediated AKT Phosphorylation Promotes RCC Tumorigenesis and Drug Resistance.

Advanced renal cell carcinoma (RCC) primarily relies on targeted and immune-based therapies, yet these treatments often face limitations due to inefficacy and drug resistance. Branched-chain α-keto-acid dehydrogenase kinase (BCKDK) has been implicated in promoting RCC metastasis, but its specific substrates and the mechanisms underlying its regulation of RCC progression remain poorly understood. This study uncovers a novel mechanism whereby BCKDK-mediated AKT phosphorylation drives RCC tumorigenesis and drug resistance.

Translation regulation in Bacillus subtilis and its applications in heterologous protein expression: A review.

Bacillus subtilis is widely used for industrial enzyme production due to its food safety and good capability of protein synthesis and secretion. However, the production of heterologous proteins is often inefficient, partly due to poor compatibility and versatility of genetic elements in B. subtilis.

Integration of steel slag and zeolite enhances simultaneous nitrification and autotrophic denitrification in ultra-low carbon/nitrogen ratio wastewater: Remodeling microbiota and iron metabolism.

Constructed wetlands (CWs) are widely used for nitrogen pollution control in rural aquatic environments, yet their nitrogen removal efficiency often remains suboptimal. This study firstly examines how zeolite robustly stimulates Fe-utilization of steelmaking waste (i.e.

High-Fidelity, High-Conductivity and Multifunctional PEDOT:PSS Hydrogel for Efficient Electromagnetic Interference Shielding and Ultrafast Response Electrochromic Applications.

Poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) hydrogel are promising for applications in electromagnetic interference (EMI) shielding, energy storage, and electrochromic (EC) devices. However, challenges such as low conductivity at reduced solid content, limited charge storage, poor mechanical properties, and structural distortion during solvent treatment limit their high-performance applications. To address these issues, a high-fidelity, high-conductivity and multifunctional PEDOT:PSS hydrogel is developed by an ice crystal-assisted skeleton stacking and stepwise treatment strategy, achieving ultrahigh conductivity of 87,249 S m at 5.

Alkalinity control in sludge propels the conversion of concrete slurry waste into micro- and nano-sized biogenic CaCO.

The utilization of for the production of bio-CaCO in concrete crack repair and strength enhancement has attracted considerable attention. However, microbial-induced calcium carbonate precipitation (MICP) has yet to be explored as a precedent with activated sludge. Here calcium sourced from concrete slurry waste (CSW) and carbon from sludge microbial β-oxidation under alkaline were used to generate micro/nano CaCO.

Application of protein engineering to ene-reductase for the synthesis of chiral compounds through asymmetric reaction.

Ene-reductase (ER) has been widely applied for asymmetrical synthesis of chiral intermediates due to its substrate promiscuity, photoexcited reactivity, and excellent property with producing two chiral centers at a time. Natural ERs often exhibit the same stereoselectivity, and they need to be engineered for opposite configuration of chiral compounds. The hydrogenation process toward activated alkenes by ERs is composed of reductive half reaction and oxidative half reaction, which are dependent upon two cofactors NAD(P)H and flavin mononucleotide.

Research on Friction Performance of Friction Stir Welding Tools Based on Non-Smooth Structure.

In this study, based on the principles of bionics, we fabricated a bionic non-smooth concave pit structure on the shoulders of friction stir welding tools and detected the thermal cycling curve, downforce, and torque of the tool in the welding process. We tested the wear loss weight and analyzed the surface morphology of the shoulder surfaces after welding for 200 m. This study found that as the distance between the concave pits decreased and the number of concave pits increased, the maximum downforce, torque, and temperature in the welding process showed a decreasing trend.

Multi-channel broadband optical chaos generation assisted by phase modulation and CFBG feedback.

In this paper, we propose a novel and simple multi-channel broadband optical chaos generation scheme based on phase modulation and chirped fiber Bragg grating (CFBG). Firstly, phase modulation is introduced to generate more new frequency components to broaden the spectrum of the phase chaos. Meanwhile, the accumulated dispersion from CFBG distorts the intensity chaos, converts phase chaos to intensity chaos, and weakens the laser relaxation oscillation.

Can three-dimensional nitrate structure be reconstructed from surface information with artificial intelligence? - A proof-of-concept study.

Nitrate is one of the essential variables in the ocean that is a primary control of the upper ocean pelagic ecosystem. Its three-dimensional (3D) structure is vital for understanding the dynamic and ecosystem. Although several gridded nitrate products exist, the possibility of reconstructing the 3D structure of nitrate from surface data has never been exploited.

Enhancing the Enantioselectivity and Catalytic Efficiency of Esterase from for Kinetic Resolution of l-Menthol through Semirational Design.

Enzymatic kinetic resolution is a promising way to produce l-menthol. However, the properties of the reported biocatalysts are still unsatisfactory and far from being ready for industrial application. Herein, a -nitrobenzylesterase (pnbA) gene from was cloned and expressed to produce l-menthol from d,l-menthyl acetate.

Experimental demonstration of 201.6-Gbit/s coherent probabilistic shaping QAM transmission with quantum noise stream cipher over a 1200-km standard single mode fiber.

A probabilistic shaping (PS) quadrature amplitude modulation (QAM) based on Y-00 quantum noise stream cipher (QNSC) has been proposed. We experimentally demonstrated this scheme with data rate of 201.6Gbit/s over a 1200-km standard single mode fiber (SSMF) under a 20% SD-FEC threshold.

Modeling of a multi-parameter chaotic optoelectronic oscillator based on the Fourier neural operator.

A model construction scheme of chaotic optoelectronic oscillator (OEO) based on the Fourier neural operator (FNO) is proposed. Different from the conventional methods, we learn the nonlinear dynamics of OEO (actual components) in a data-driven way, expecting to obtain a multi-parameter OEO model for generating chaotic carrier with high-efficiency and low-cost. FNO is a deep learning architecture which utilizes neural network as a parameter structure to learn the trajectory of the family of equations from training data.

Mechanical Properties and Coagulation Characteristics of Flue Gas Desulfurization Gypsum-Based Polymer Materials.

To resolve problems caused by the accumulation of flue gas desulfurization gypsum (FGDG) in the environment, a polymer material was prepared using FGDG, granulated blast furnace slag (GBFS), fly ash (FA), and solid sodium silicate (SSS). The compressive strength of these polymer specimens cured for 3, 28, and 60 d was regularly measured, and their condensation behavior was analyzed. Both the formation behavior of mineral crystals and microstructure characteristics were analyzed further using X-ray diffraction and scanning electron microscopy.

Application of nanoarchitectonics in moist-electric generation.

The consumption of energy is an important resource that cannot be ignored in modern society. Non-renewable forms of energy, such as coal, natural gas, and oil, have always been important strategic resources and are always facing a crisis of shortage. Therefore, there is an urgent need for green renewable forms of energy.

In Situ Encapsulated Moiré Perovskite for Stable Photodetectors with Ultrahigh Polarization Sensitivity.

Nanostructures provide a simple, effective, and low-cost route to enhance the light-trapping capability of optoelectronic devices. In recent years, nano-optical structures have been widely used in perovskite optoelectronic devices to greatly enhance the device performance. However, the inherent instability of perovskite materials hinders the practical application of these nanostructured optoelectronic devices.

Coagulation Mechanism and Compressive Strength Characteristics Analysis of High-Strength Alkali-Activated Slag Grouting Material.

In deep coal mining, grouting reinforcement and water blockage are the most effective means for reinforcing the rock mass of extremely broken coal. However, traditional cement grouting materials are not suitable for use in complex strata because of their insufficient early mechanical strength and slow setting time. This study innovatively proposes using alkali-activated grouting material to compensate for the shortcomings of traditional grouting materials and strengthen the reinforcement of extremely unstable broken coal and rock mass.

Preparation and Properties of Municipal Solid Waste Incineration Alkali-Activated Lightweight Materials through Spontaneous Bubbles.

A self-foaming alkali-activated lightweight material was prepared by the pretreatment of municipal solid waste incineration bottom ash (BA). The low weight could be achieved without adding a foaming agent by using the low-density and self-foaming expansion characteristics of BA in combination with a strong alkali. The effects of BA, liquid sodium silicate (LSS), and calcium hydroxide (CH) on dry and wet densities, as well as water absorption, are discussed.

Chaotic optical communications at 56 Gbit/s over 100-km fiber transmission based on a chaos generation model driven by long short-term memory networks.

Chaotic optical communication technology is considered as an effective secure communication technology, which can protect information from a physical layer and is compatible with the existing optical networks. At present, to realize long-distance chaos synchronization is still a very difficult problem, mainly because well-matched hardware cannot always be guaranteed between the transmitter and receiver. In this Letter, we introduce long short-term memory (LSTM) networks to learn a nonlinear dynamics model of an opto-electronic feedback loop, and then apply the trained deep learning model to generate a chaotic waveform for encryption and decryption at the transmitter and receiver.

Trading off security and practicability to explore high-speed and long-haul chaotic optical communication.

Recent demonstrations of chaos-based secure communication have proven the feasibility of secured transmission of high-speed (tens of Gbit/s) signals over certain distances (∼100-km), which bring hope for secure communication from theoretical analysis to practical applications. So far, the chaos-based secure communication system with chaos-masking (CMS) encryption is considered as one of the most important and feasible schemes. In this paper, an optical chaotic carrier generated by an opto-electronic oscillator is used to encrypt 112-Gbit/s message by CMS encryption for data transmission over a 1040-km single-mode-fiber.

[Construction and Application Optimization of the Chl-a Forecast Model ARIMA for Lake Taihu].

As an important indicator of phytoplankton biomass in lakes, the chlorophyll-a (Chl-a) concentration reflects the abundance and variation of phytoplankton in the water. Based on the monthly monitoring data of Chl-a and environmental factors in Lake Taihu from December 1999 to August 2019, key environmental factors related to Chl-a and their relationships were found using the principal component analysis (PCA) method. A multiple linear stepwise regression model and an auto-regressive integrated moving average (ARIMA) model were developed to predict the monthly Chl-a concentrations.

Experimentally validated full-vectorial model of wavelength multicasting via four-wave mixing in straight waveguides.

We derive full-vectorial nonlinear propagation equations of dual-pumped four-wave mixing in straight waveguides, which are valid in characterizing the one-to-six wavelength multicasting. Special attention is paid to the resulting idler wavelengths and their conversion efficiency, which enables the optimization of the experimental designs, including the incident wavelength and the power of pumps and signal. We validate the model by comparing the numerical simulation to the experimental measurement in a silicon-on-insulator waveguide, for the first time to our best knowledge, and achieve a good agreement.

[Effect of specific immunotherapy on GM-CSF and IL-5 in the tissues of recurrent nasal polyps].

Objective: To study the mechanism and clinical significance of specific immunotherapy (SIT) on the expression changes of GM-CSF and IL-5 in the tissue samples of recurrent nasal polyps.

Method: Perennial allergic rhinitis patients with recurrent nasal polyps were randomly divided into 2 groups. The experimental group of 19 patients was treated by SIT and standardized treatment (glucocorticoid nasal spray) , and the control group of 17 patients was only treated by standardized treatment (glucocorticoid nasal spray).

Endoscopic therapy of colonic liver flexure mucocele.

Colorectal mucoceles usually arise in the appendix, and colonic disease is very rare. We report the first case of a mucocele of the colonic liver flexure that was treated successfully with endoscopy. A 36-year-old man was admitted to our hospital because of abdominal distension persisting for 3 days.