Phytochrome B stabilizes the KNOX transcription factor BP/KNAT1 to promote light-initiated seed germination in Arabidopsis thaliana.

Seed germination is a critical step in the life cycle of plants. The far-red/red light photoreceptor phytochrome B (phyB) plays a dominant role in promoting seed germination, mainly by modulating the metabolism of gibberellin (GA) and abscisic acid (ABA), although the underlying mechanism remains poorly understood. In this study, we identified BREVIPEDICELLUS (BP)/KNAT1, a KNOX transcription factor that acted downstream of phyB and activated light-initiated seed germination in Arabidopsis thaliana.

Differential gene expression drives muscle metabolic and structural differences in Liang Guang small spotted vs. large white pigs.

The Liang Guang Small Spotted (LGSS) pigs, an indigenous Chinese breed, are reported to exhibit desirable muscle attributes such as high intramuscular fat deposition, smaller muscle fiber diameter, and superior water-holding capacity. However, despite these merits, its commercial utility is constrained by suboptimal growth rates and diminished carcass yield. To explore transcriptomic differences potentially associated with distinct muscle phenotypes and identify targets for productivity enhancement, we performed integrated transcriptomic analysis of longissimus dorsi muscles (LDMs) from LGSS and Large White (LW) pigs.

Incidence and risk factors for modic changes in the lumbar spine: a systematic review and meta-analysis.

Background: Modic changes refer to bone marrow alterations beneath vertebral endplates and are potentially linked to infection, trauma, disc degeneration, scoliosis, and other pathological conditions. Systematic evaluations of their incidence and associated risk factors in the lumbar spine are lacking. This study aimed to analyze and evaluate the incidence and risk factors of Modic changes in lumbar spine disorders.

HDT2-mediated lysine deacetylation promotes phytochrome A degradation during photomorphogenesis in Arabidopsis.

The shift from skotomorphogenesis to photomorphogenesis, a developmental transition in seed plants, involves dramatic proteomic changes. Lysine acetylation (Lys-Ac) is an evolutionarily conserved and recognized post-translational modification that plays a crucial role in plant development. However, its role in seedling deetiolation remains unclear.

UniAV: Unified Audio-Visual Perception for Multi-Task Video Event Localization.

Video event localization tasks include temporal action localization (TAL), sound event detection (SED) and audio-visual event localization (AVEL). Existing methods tend to over-specialize on individual tasks, neglecting the equal importance of these different events for a complete understanding of video content. In this work, we aim to develop a unified framework to solve TAL, SED and AVEL tasks together to facilitate holistic video understanding.

Assessing Discharge Readiness and Factors in Peripherally Inserted Central Catheter Carriers with Malignant Tumors: A Meta-Analysis.

Background: The aim of this meta-analysis was to assess the readiness for discharge of patients with malignancy when carrying a peripherally inserted central catheter (PICC) as well as to explore the associated factors affecting readiness for discharge.

Methods: PubMed, EMBASE, Web of Science, CNKI, WanFang and VIP databases from inception to Mar 2024 were systematically searched to collect relevant cross-sectional studies. Fixed-effects and random-effects models were used for effect size synthesis, and the stability of the results was assessed by heterogeneity testing, sensitivity analysis, and publication bias detection.

Epithelial characteristics of ovarian clear cell carcinoma at single-cell resolution.

Ovarian clear cell carcinoma (OCCC) represents a rare and aggressive subtype of epithelial ovarian cancer with distinctive clinical and molecular characteristics. However, the identification, origin, and molecular features of the malignant epithelial cells in OCCC remain poorly studied. We establish an OCCC-associated transcriptional landscape using single-cell RNA sequencing and investigated the properties of epithelial cells in tissues from normal ovaries, ovarian endometriosis, primary OCCC and recurrent OCCC to assess the status of malignant epithelial cells.

Integrated physiological, transcriptomic and metabolomic analysis of the response of Brassica napus L. to pruning residues from tea plant.

In the process of tea garden management, the pruned tea plant branches usually return directly to the field, which may interfere adjacent plant growth and resource waste. Therefore, it is essential to properly manage and economically utilize pruned tea plant branches. This study was combined physiology, transcriptomics, and metabolomics to analyze the effects of uncomposted and composted tea plant branches (UT and CT, respectively) on seed germination and seedling growth of Brassica napus L.

Role of d-serine in intestinal ROS accumulation after sleep deprivation.

Prolonged sleep deprivation (SD) results in increased accumulation of reactive oxygen species (ROS) in gut, although the underlying mechanisms remain to be elucidated. This study identifies d-serine as a crucial regulator of gut ROS during SD. Knockdown of serine racemase (SR), the enzyme responsible for d-serine production, prevents the enhanced ROS buildup during SD in .

Association of follow-up neutrophil-lymphocyte ratio with postoperative pneumonia in aneurysmal subarachnoid hemorrhage patients after endovascular treatment: a retrospective analysis.

Introduction: Research on the relationship between dynamic neutrophil-to-lymphocyte ratio (NLR)-measured at different time points around endovascular treatment-and the occurrence of postoperative pneumonia (POP) in patients with aneurysmal subarachnoid hemorrhage (aSAH) is still relatively scarce. This study aims to explore the association between dynamic NLR and the incidence of POP in patients with aSAH undergoing endovascular therapy.

Methods: This study included patients with aSAH who underwent endovascular treatment between January 2019 and April 2023.

Targeted Europium Chelation: A Strategy for Intervening Tumor Extracellular Matrix.

As an important component of the tumor microenvironment, the extracellular matrix (ECM) generally undergoes extensive remodeling, acting as a shield to block drug delivery and immune recognition. Currently, ECM-clearing strategies for tumor treatment still face challenges. In this work, a type of europium metal-organic nanowire was prepared for targeting the highly expressed sialic acid (SA) on glycoproteins within the ECM.

Fluorescent probes for the visualization of membrane microdomain, deformation, and fusion.

The cell membrane, a fluid interface composed of self-assembled phospholipid molecules, is a vital component of biological systems that maintains cellular stability and prevents the invasion of foreign toxins. Due to its inherent fluidity, the cell membrane can undergo bending, shearing, and stretching, making membrane deformation crucial in processes like cell adhesion, migration, phagocytosis, and signal transduction. Within the plasma membrane are highly ordered dynamic structures formed by lipid molecules, known as "lipid rafts," whose dynamic dissociation and reorganization are prerequisites for membrane deformation.

Multi-omics analyses provide insights into the molecular basis for salt tolerance of Phyla nodiflora.

The perennial herbaceous plant, Phyla nodiflora (Verbenaceae), which possesses natural resistance to multiple abiotic stresses, is widely used as a pioneer species in island ecological restoration. Due to the lack of information about its genome, the mechanism underlying its tolerance to environmental stresses, such as salinity, is almost entirely unknown. Here, we report on the high-quality genome of P.

The processed L. triterpenoids prevent colon cancer through the TNF-/NF-κB signaling pathway, combined with network pharmacology, molecular simulation dynamics and experimental verification.

Background: L. (S.L.

Interpretable Machine Learning Prediction of Polyimide Dielectric Constants: A Feature-Engineered Approach with Experimental Validation.

Low-dielectric polyimides (PIs) have emerged as essential materials for next-generation microelectronics and communication technologies, yet traditional experimental and theoretical calculation methods for acquiring dielectric constant data face challenges in cost, accuracy, and scalability. This study presents a machine learning (ML) framework that combines polymer domain knowledge with advanced data-driven modeling techniques for accurate prediction of PI dielectric constants at 1 kHz. A dataset of 439 PIs was constructed, and 208 molecular descriptors were derived from SMILES-encoded structures.

Quantitative Evaluation and Preoperative Planning Using Three-Dimensional CT Profiles Improves the Accuracy of Bony Correction Under Primary Hip Arthroscopy and Clinical Outcomes in the Setting of Cam-Type Femoroacetabular Impingement Syndrome.

Objective: Residual cam deformity is the main cause of revision hip arthroscopy, and the precise correction of cam lesions is a crucial clinical problem. This study aimed to propose a novel quantitative evaluation and preoperative planning method based on 3D-CT profile analysis applied in primary hip arthroscopy for the treatment of cam-type femoroacetabular impingement syndrome (FAIS) and to evaluate the effect of this method.

Methods: Consecutive patients who underwent primary hip arthroscopy for cam-type FAIS between April 2018 and August 2022 were enrolled in this study.

Sex hormones and epigenetic dysregulation in autoimmune disease.

Healthy individuals exhibit sex-specific immune responses. In certain immune-related diseases, such as autoimmune diseases, there is a pronounced sex bias in incidence, severity, and other clinical features. These differences are influenced by immune-related genes encoded in the sex chromosomes, as well as by genes whose expression is regulated by sex hormones.

Dynamic changes in volatile aroma compounds during Chinese Xiaoqu Baijiu brewing.

Baijiu aroma has become an increasingly popular topic in the study of aroma compounds. However, the changes in the volatile aroma compounds of Chinese Xiaoqu Baijiu during the brewing process remain unclear. In this study, headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was employed to analyze the flavor components throughout the brewing process of Chinese Xiaoqu Baijiu.

Gitelman syndrome with hypercalcemia and normomagnesemia: A case report.

Rationale: Gitelman syndrome (GS) is a rare autosomal recessive renal tubular disease, whose main symptoms are long-term hypokalemia, hypomagnesemia, hypochloremic metabolic alkalosis, and hypocalciuria.

Patient Concerns: This study reported a GS patient with hypercalcemia combined with normomagnesemia.

Diagnoses: GS with hypercalcemia.

Clinical Impact of Sub-Clonal RAS/BRAF Alterations in Liquid Biopsies From Patients With Advanced or Metastatic CRC.

Introduction: Colorectal cancer (CRC), a global health concern, requires effective treatments. Anti-epidermal growth factor receptor (anti-EGFR) monoclonal antibodies are used for RAS wild-type and BRAF mutation-negative metastatic CRC (mCRC) but are not indicated for RAS mutant CRC. Evidence suggests CRC patients with sub-clonal RAS/BRAF mutations in tumor tissue may benefit from anti-EGFRs.

Ultrasound-assisted combined with natural deep eutectic solvents for Platycodon grandiflorum polysaccharides extraction: Process optimization and evaluation of anti-lung cancer activity.

Platycodon grandiflorum (PG) is a traditional medicinal herb with a history of >2000 years, exhibiting various biological activities. However, its anti-lung cancer potential remains largely unexplored. This study aimed to extract Platycodon grandiflorum polysaccharides (PGP) using an environmentally friendly natural deep eutectic solvent (NADES) system and evaluate their anti-lung cancer activity.

Finite element analysis of neck sports injury based on a whole cervical spine model with muscles.

Cervical atlantoaxial subluxation injuries, often resulting from high-intensity external forces or improper posture during high-speed, rotational sports, pose significant risks to athletes' health and careers. This study aims to investigate the biomechanical effects of atlantoaxial subluxation on the cervical spine. Models representing Model 1 (healthy bone model), Model 2 (healthy muscle model), and atlantoaxial subluxation diseased model were developed using CT and MRI data.

Advances and challenges in lipid droplet isolation from animal tissues and cells.

Lipid droplets (LDs) are essential intracellular organelles involved in lipid storage and metabolism, playing critical roles in various cellular processes and diseases. Researchers require efficiently isolate and analyze LDs to understand lipid metabolism and related pathologies. This review summarizes recent advances in LD isolation methods, including traditional techniques such as centrifugation and density gradient centrifugation, as well as emerging technologies like automated and high-throughput approaches.

Recent Advances in Research on Inhibitory Effects of Seaweed Extracts Against Parasites.

Parasitic diseases pose a serious threat to the health of humans and the steady development of livestock husbandry. Although there are certain drug-based treatment methods, with the widespread application of drugs, various parasites are gradually developing drug resistance. Natural products are highly favored by researchers due to their characteristics such as low toxicity, multi-target effects, and low risk of drug resistance.