LncRNA LINC01123-mediated impaired fracture healing through miR-134-5p/XIAP axis.

Background: The prolonged recovery and heightened discomfort associated with delayed fracture healing present substantial clinical challenges. Investigations into lncRNA-mediated pathways may provide novel biological targets for enhancing osseous regeneration and improving clinical outcomes.

Aim: The focus of this study is to elucidate the role and molecular mechanism of lncRNA LINC01123 (LINC01123) dysregulation in the osteogenic differentiation of osteoblasts.

Involvement of endocannabinoid receptor 1 in oxidative and inflammatory responses underlying anxiety-like behavior in SPS&S-exposed mice.

Objective: This study investigated the role of endocannabinoid (eCB) signaling in anxiety-like behaviors associated with a PTSD mouse model (SPS&S), focusing on oxidative stress and inflammation.

Methods: We examined expression of eCB-related proteins (Cannabinoid receptor type 1, CB1 Receptor; NAPE-specific phospholipase D, NAPE-PLD; Fatty acid amide hydrolase, FAAH; and Monoacylglycerol lipase, MAGL) and inflammatory markers (IL-1β, caspase-1) in the mPFC, hippocampus, and amygdala of SPS&S-exposed mice. Plasma oxidative stress markers (MDA, SOD, ROS) and cytokines (IL-1β, IL-6, TNF-α, COX-2) were measured by ELISA.

The MdWRKY50-MdHD14 Module Regulates Fruit Senescence by Affecting γ-Aminobutyric Acid Synthesis.

Fruit senescence negatively affects apple quality and postharvest storage. γ-Aminobutyric acid (GABA) accumulates rapidly following stress. Here, we found that the HD-ZIP transcription factor (TF) MdHD14 induces GABA accumulation by upregulating MdGAD2 and MdGAD4.

Apparent charge reduction in multipolarons crafted one-by-one in monolayer CrBr.

Manipulating electrons opens up emerging synthetic strategies. Multipolaron, as a rare quasiparticle containing multiple excess charges collectively dressed with shared local lattice distortions, provides an ideal medium for electron manipulation, yet remains elusive. Here, with scanning tunneling microscopy, we realize electron multipolarons in monolayer CrBr.

Influencing factors and trends in post-vaccination serologic testing completion rates among infants born to HBsAg-positive mothers in Guangdong Province, 2021-2023.

Objective: This study aims to assess the trends and influencing factors in post-vaccination serologic testing (PVST) completion rates among infants born to hepatitis B surface antigen (HBsAg)-positive mothers using real-world data, providing insights to guide future PVST strategy implementation.

Methods: Data were obtained from the case records of HBsAg-positive pregnant women and their newborns in Guangdong Province from January 1, 2021, to December 31, 2023. The Cochran-Armitage test was used to analyze trends in PVST completion rates and the age distribution of infants completing PVST, among HBV-exposed neonates, stratified by birth weight, from 2021 to 2023.

Integrated physiological, transcriptomic and metabolomic analysis of the response of Brassica napus L. to pruning residues from tea plant.

In the process of tea garden management, the pruned tea plant branches usually return directly to the field, which may interfere adjacent plant growth and resource waste. Therefore, it is essential to properly manage and economically utilize pruned tea plant branches. This study was combined physiology, transcriptomics, and metabolomics to analyze the effects of uncomposted and composted tea plant branches (UT and CT, respectively) on seed germination and seedling growth of Brassica napus L.

RinRK1's extracellular domain acts as a host-specific gatekeeper for rhizobial infection in Medicago truncatula.

Legume nodulation by nitrogen-fixing rhizobia displays strict host specificity, primarily determined by rhizobial nodulation factors (NFs). While the kinase domain of NF receptors in Lotus japonicus suffices for nodule organogenesis, their extracellular domains govern rhizobial infection. In Sinorhizobium meliloti, specific NF modifications (nodL-dependent acetylation and nodF-dependent N-linked C16:2 acyl chains) are critical for infecting certain Medicago truncatula ecotypes, with LYK2bis essential for nodL-mediated infection.

Elephantopus scaber attenuates MC903-caused atopic dermatitis and decreases TNF-α/IFN-γ-induced chemokines by suppressing MKP-1-mediated AP-1 signaling in keratinocytes.

Ethnopharmacological Relevance: Atopic dermatitis (AD) is an inflammatory skin disease. Elephantopus scaber L. (E.

The component-to-trait relationship between intolerance of uncertainty and obsessive-compulsive personality traits: a network analysis.

Background: Previous studies examining the relationship between obsessive-compulsive personality traits (OCPT) and intolerance of uncertainty (IU) at the latent variable level (using total score analyses) have demonstrated positive correlations between them. However, such aggregate-level analyses obscure potential differential associations among specific OCPT and IU components.

Methods: This study employed network analysis to investigate the correlation patterns between IU and OCPT at the component/trait level in a sample of 1,440 Chinese college students.

Beneficial Effects of N102 and H1-5 Added as Starter Strains on the Metabolome, Safety and Quality of Dry-Fermented Sausages.

This study investigated the beneficial effects of individual and co-inoculation with N102 and H1-5 on improving safety parameters, sensory characteristics, and non-volatile metabolite profiles in dry-fermented sausages. Comprehensive analyses were conducted throughout the 20-day maturation period (0, 6, 13, 16, and 20 days), including physicochemical monitoring (moisture content, malondialdehyde (MDA) levels, biogenic amine concentrations, and sodium nitrite residues); sensory evaluation (color parameters and textural properties); and H NMR-based metabolomic profiling. Key findings revealed strain-specific advantages: the N102 inoculation significantly delayed lipid oxidation, achieving the lowest final MDA concentration (4.

Expression and clinical significance of mir-520g-3p in patients with gestational diabetes mellitus.

Background: Gestational diabetes mellitus (GDM) is a metabolic disorder characterized by glucose intolerance that develops during pregnancy. Recent studies have begun to elucidate the role of MicroRNAs (miRNAs) in the pathophysiology of GDM, suggesting that these non-coding RNAs could serve as potential biomarkers for the condition. This study aimed to investigate the clinical diagnostic value of miR-520g-3p in GDM patients.

Mechanistic Insights into the Anti-Glioma Effects of Exosome-Like Nanoparticles Derived from L.: A Metabolomics, Network Pharmacology, and Experimental Study.

Introduction: L. a widely used tropical fruit, has been historically valued as a medicinal plant. Modern pharmacological research has identified several compounds in its pericarp, such as alkaloids, flavonoids, and phenolic acids, which possess antioxidant and anticancer properties.

Concurrent TMS-EEG to characterize cortical responses in the motor and prefrontal cortices in Parkinson's disease.

Patients with Parkinson's disease (PD) experience both motor and non-motor symptoms. However, it remains unclear the full spectrum of PD, which requires a comprehensive assessment of both motor and non-motor cortical regions. The use of combined transcranial magnetic stimulation and electroencephalography (TMS-EEG) allows the examination of neural circuit beyond motor cortex.

Design and validation of novel brain-penetrant HCN channel inhibitors to ameliorate social stress-induced susceptible phenotype.

Major Depressive Disorder (MDD) is a devastating, multifactorial disease with limited pharmacological treatment options. Patients with MDD exhibit alterations in their dopamine (DA) signaling pathways through the midbrain ventral tegmental area (VTA). A similar observation is also detected in preclinical models of stress - mice exhibit behavioral and physiological impairments following chronic social defeat stress (CSDS).

Effects of fecal microbiota transplantation from patients with generalized anxiety on anxiety-like behaviors: The role of the gut-microbiota-endocannabinoid-brain Axis.

Background: Intestinal dysbacteriosis is frequently implicated in generalized anxiety disorder (GAD). However, the molecular mechanisms and functional changes of the gut-brain axis in GAD remain largely unexplored.

Methods: We investigated anxiety-like behaviors, gut microbiota changes, brain region-specific endocannabinoid (eCB) system alterations, including the expression of cannabinoid type 1 (CB1R), monoacylglycerol lipase (MAGL), and fatty acid amide hydrolase (FAAH) in the hippocampus (Hip), prefrontal cortex (PFC), and amygdala (Amy), as well as plasma medium- and long-chain fatty acids (MLCFAs) in a mouse model of chronic restraint stress (CRS) and antibiotic-treated mice receiving fecal microbiota transplantation from GAD patients (FMT-GAD).

Association Between Bovine Aortic Arch Anatomy and Stroke After Thoracic Endovascular Aortic Repair.

Objective: The link between bovine aortic arch (BAA) and stroke after thoracic endovascular aortic repair (TEVAR) was investigated.

Methods: The multicenter retrospective study included consecutive Stanford type B aortic dissection patients undergoing TEVAR from January 2019 to December 2023. A multivariable logistic regression was utilized to assess the correlation between BAA and early stroke, while a multivariable Cox regression was employed to evaluate the association between BAA and overall stroke.

S-ketamine exposure in early postnatal period induces social deficit mediated by excessive microglial synaptic pruning.

The impact of general anesthetics on neurodevelopment is highly controversial in terms of clinical and preclinical studies. Evidence mounted in recent years indicated development of social cognitions was more susceptible to general anesthesia in early life. However, the behavioral characterization during adolescence and underlying mechanisms remains unclear.

Risk factors for rifampicin-susceptible and isoniazid-resistant tuberculosis in adult patients with type 2 diabetes mellitus in Nanjing.

Objective: Globally, Tuberculosis(TB) with type 2 diabetes mellitus (T2DM) is becoming increasingly serious, especially the emergence of rifampicin-susceptible and isoniazid-resistant tuberculosis (Hr-TB), which increases the difficulty of treatment and the burden of disease. Therefore, this single-center retrospective cohort study analyzed risk factors of Hr-TB in adult patients with T2DM and pulmonary tuberculosis (PTB) in Nanjing to guide clinical practice and improve the long-term prognosis of patients.

Methods: The clinical data of 279 adult inpatients diagnosed with culture-positive PTB and T2DM in the Second Hospital of Nanjing from January 2019 and December 2021 were collected.

ErTao decoction alleviates liver fibrosis by suppressing STING-mediated macrophages and NLRP3 inflammasome activation.

Background: Liver fibrosis (LF) is a common pathological process in the progression of multiple chronic liver diseases to cirrhosis, affecting millions of people worldwide annually. The incomplete understanding of its mechanisms has led to a lack of clinically effective therapeutic options. ErTao decoction (ETD, ), a derivative combining the components of Erchen Decoction and Taohong Siwu Decoction, is rooted in the traditional Chinese medicine theory of "phlegm-dampness-blood stasis".

Construction and Validation of a Predictive Nomogram Model for Patients with Type 2 Diabetes Complicated by Diabetic Foot Ulcers.

To investigate the risk factors for diabetic foot ulcers (DFU) in patients with type 2 diabetes (T2DM), to create a nomogram prediction model, and to further validate the nomogram model. 500 T2DM patients treated at Shanghai Integrated Traditional Chinese and Western Medicine Hospital were retrospectively analyzed from April 2023 to November 2023. Patients were categorized into groups based on the presence of DFU (n = 64) and T2DM (n = 436).

Related factors of turnover intention among general practitioners: a cross-sectional study in 6 provinces of China.

Background: Turnover among general practitioners (GPs) is an issue of growing concern. Therefore, identification of the variables which associated with GPs' decision in staying in or leaving their employer institutions is necessary.

Objective: The aim of this study was to investigate the relationship between work stress, job satisfaction, and turnover intention, and explore factors associated with turnover intention of GPs in China.

Polaron formation by electron polarization in two-dimensional transition metal halides.

An electron in solid can be dressed by the lattice distortions of surroundings, forming a localized composite quasiparticle called small polaron, whose formation has been customarily attributed to the electron-phonon couplings that the ion polarization traps the excess electron. Here we present a theory of electron-polarization induced small polaron, in which the carrier localization happens spontaneously and drives subsequent ion relaxation. This mechanism of polaron formation is qualitatively different than the Mott-Stoneham picture in that there is no need to overcome a kinetic barrier for the carrier to self-trap to form a polaron.

Effect of minimally invasive strabismus surgery under a microscope on tear film function and ocular surface status in strabismus patients.

Objective: To investigate the effects of minimally invasive strabismus surgery (MISS) on tear film function and ocular surface status in patients with strabismus.

Methods: We respectively analyzed the clinical data from 173 cases of strabismus patients treated at Xi'an First Hospital from September 2021 to March 2024. The patients were classified into a minimally invasive group (n=91, undergoing MISS) and a conventional group (n=82, undergoing traditional strabismus correction) according to their treatment plans.