Upregulation of EMP3 in acute myeloid leukemia: a study based on data mining, RT-qPCR and immunohistochemistry.

Background: Epithelial Membrane Protein 3 (EMP3) has been associated with multiple malignancies, but its expression patterns and clinical significance in acute myeloid leukemia (AML) remain poorly characterized.

Methods: Public datasets were integrated to assess EMP3 mRNA expression levels in AML patients versus healthy donors, with validation performed using reverse transcription quantitative PCR (RT-qPCR). Protein expression was accessed through immunohistochemistry.

Integration of an AIE photosensitizer and a COX-2 inhibitor for synergistic and enhanced tumor therapy.

Photodynamic therapy (PDT) has emerged as a minimally invasive and precision-guided cancer treatment method. However, single-modality PDT is limited by incomplete tumor ablation, and paradoxical immune evasion triggered by inflammatory cascades. Herein, we present BTDA-IMC, a nanoplatform that integrates an aggregation-induced emission (AIE) photosensitizer with a cyclooxygenase-2 (COX-2) inhibitor to synergistically enhance PDT efficacy.

Overexpression of promotes cell metabolism and activates the NOTCH signaling pathway in lung adenocarcinoma.

Background: Lung adenocarcinoma (LUAD) is one of the most malignant types of cancer with a high incidence. Protein phosphatase, Mg/Mn-dependent 1G () has recently been reported to promote cancer progression; however, its specific role in LUAD remains unknown. Therefore, in this study, we aimed to clarify the roles of in LUAD and the related the regulatory mechanisms.

Themis differentially regulates T follicular helper cell differentiation during early and late stages of chronic viral infection.

Introduction: T follicular helper (TFH) cells are critical for humoral immunity during chronic viral infection, but the mechanisms guiding their differentiation from a novel CD4⁺ T cell progenitors remain incompletely understood. Themis, a T cell-specific adaptor protein, has been implicated in T cell development and function, but its role in peripheral CD4⁺ T cell differentiation under chronic antigen stimulation has not been defined.

Methods: We used a chronic lymphocytic choriomeningitis virus (LCMV) Clone13 infection model in wild-type and Themis conditional knockout (cKO) mice.

Sulfide-driven denitrification process for low C/N ratio wastewater treatment.

This study presented a novel strategy employing sulfide (NaS) as the external electron donor and enzyme activity inhibitor to enhance nitrogen removal and enable nitrous oxide (NO) recovery in low carbon-to-nitrogen (C/N) ratio wastewater treatment. With the addition of NaS, the system achieved a maximum total nitrogen (TN) removal of 100.0 % while accumulating nitrogen oxide (NO) and elemental sulfur (S) at an average yield of 60.

A bibliometric and visual analysis of Jak1 to explore trends and frontiers.

Background: JAK1, a member of the JAK kinase family, is involved in the signal transduction of multiple cytokine pathways and is crucial in the onset and progression of inflammation and tumours. Consequently, JAK1 has garnered significant attention in recent years.

Methods: We use bibliometric and visual analysis to evaluate the thematic trends and knowledge structure of TRPV1's research papers on JAK1, sourced from the Web of Science core collection from 2003 to 2024.

Hypotrichosis 14: novel variants of the LSS gene in five Chinese families and insights from literature review.

Background: Congenital hypotrichosis 14 is a nonsyndromic form of alopecia associated with pathogenic variants in the lanosterol synthase (LSS) gene. Recent studies have expanded the spectrum of LSS-related phenotypes, including congenital cataracts, alopecia-intellectual disability syndrome, and palmoplantar keratoderma. Currently, investigations into this disease are still limited, and its treatment remains elusive.

RF-URL 2.0: A General Unsupervised Representation Learning Method for RF Sensing.

The major challenge in learning-based RF sensing is acquiring high-quality large-scale annotated datasets. Unlike visual datasets, RF signals are inherently non-intuitive and non-interpretable, making their annotation both time-consuming and labor-intensive. To address this challenge, we propose RF-URL 2.

Fe-Polyphenol Self-Assembled Nanoplatform for Sonodynamic-Ferroptosis-Autophagy Inhibition Synergistic Tumor Therapy.

Effective tumor treatment still faces tough challenges. Sonodynamic therapy (SDT) has gained recognition as a viable substitute for photodynamic therapy, providing enhanced tissue penetration and reduced skin damage by generating reactive oxygen species (ROS) via ultrasound-triggered sonosensitizers. Nonetheless, the therapeutic efficacy of SDT is often hindered by the intrinsic apoptosis resistance of cancer cells.

Evaluation of Au Nanoparticle Catalysts Supported on Metal Oxide for the Catalytic Reduction of 4-NP.

4-Nitrophenol (4-NP) pollution in the chemical, dye, and other industries poses significant risks to humans and aquatic organisms. In this study, we prepared metal oxide-supported gold nanoparticles (AuNPs/AlO, AuNPs/TiO, and AuNPs/ZnO) and applied them to the catalytic reduction of 4-NP. The Bader charge and differential charge, dos and d band centers, work function, and reaction free energy of AuNP composites were analyzed through DFT calculations.

What's holding back the making of Living Wills? A qualitative study based on stakeholder perspectives.

Objective: To analyze challenges and complexities associated with Living Wills (LWs) under current medical regulations from the perspectives of diverse stakeholders, and to inform evidence-based strategies for improving LWs implementation in Asian and global contexts.

Methods: This phenomenological study conducted semi-structured interviews with stakeholders involved in the LWs making process in Shenzhen, China (July 2023-July 2024). A hybrid snowball-purposive sampling strategy was adopted.

Microglia in the Rostral Ventromedial Medulla Mediate Synaptic Pruning via the C1q/C3-CR3 Signaling Pathway-A Mechanism for the Chronic Orofacial Pain.

The mechanism of chronic orofacial pain was investigated by examining the interaction between activated microglia, C1q, and neurons in the rostral ventromedial medulla (RVM) of rats with orofacial pain caused by temporomandibular joint injection of complete Freund's adjuvant (CFA). The results demonstrated that the pain threshold in the CFA group exhibited a continuous decline, reaching its lowest point on the third day. During the modeling process, administered daily stereotactic injections of ANX-005 and minocycline into the RVM, which resulted in a notable recovery in the rats' pain threshold and a significant increase in C1q/C3 and microglia in the RVM of CFA rats.

Study on the macro and micro characteristics of the influence of deviator stress on true triaxial unloading failure.

This paper uses a specific underground tunnel project in the eastern coastal region of China as its research background. It analyzes the impact of deviatoric stress on the evolution of rock unloading failure, providing data to support the understanding of dynamic failure during the excavation and construction of underground chambers. The macroscopic characteristics observed in true triaxial compression tests with confining pressure unloading revealed that during unloading, the ε curve initially dropped before rising back along the original loading path.

YTHDF1 differentiates the contributing roles of mTORC1 in aging.

The mechanistic target of rapamycin (mTOR) serves as an essential hub in sensing metabolic stress and regulating aging, although the differential contributions of mTOR-regulated protein and cholesterol synthesis are unclear. Post-transcriptional modifications of mRNAs, such as N6-methyladenosine (m6A), occur rapidly in response to acute environmental changes to maintain tissue homeostasis. Here, we showed that loss of YTH m6A RNA-binding protein 1 (YTHDF1) accelerated murine aging.

Development and validation of a predictive model for depression risk in Chinese obese adults.

Objective: To construct a prediction model for the risk of depression in the obese population, aiming to facilitate the early identification of high-risk individuals and guide personalized preventive interventions.

Methods: This study was based on the data from the China Health and Retirement Longitudinal Study (CHARLS 2015), the Center for Epidemiologic Studies Depression Scale-10 (CES-D10) to assess the depression of obese patients, Lasso regression and multivariable logistic regression were used to select predictors, the construction of a nomogram model, and the use of the random splitting method divided into a training set ( = 974) and a validation set ( = 418) by the 7:3 method, and the model was evaluated by the ROC curves and the AUC, the H-L goodness-of-fit test, the calibration graphs, and the clinical decision-making curve to assess the model.

Results: A total of 1,392 obese patients were finally included, with a prevalence of depression of 32.

Deciphering the multifaceted role of in female-related cancers: implications for prognosis and therapeutic responsiveness.

Background: Aberrant function or overactivation of exonuclease 1 () may be associated with cancer tumor development, drug resistance, and response to immunotherapy in female-related cancers.

Methods: By analyzing RNA-sequencing data from The Cancer Genome Atlas database, combined with validation through quantitative polymerase chain reaction experiments, we explored the expression levels of in breast cancer (BRCA) cell lines and assessed its multidimensional roles in various female-related cancers.

Results: Our experiments revealed elevated expression of in BRCA cell lines, consistent with the RNA-sequencing data.

Exploring the causal link between serum 25-hydroxyvitamin D concentrations and idiopathic sudden sensorineural hearing loss: Insights gained from a Mendelian randomization study involving two independent samples.

Idiopathic sudden sensorineural hearing loss (ISSNHL) is defined by the rapid onset of hearing impairment without an identifiable etiology. The decreased serum concentrations of 25-hydroxyvitamin D(25(OH)D) was shown to be associated with decreased hearing ability. However, current cross-sectional studies have only demonstrated an association, failing to establish a causal link.

NLRP3 inflammasome inhibits mitophagy during the progression of temporal lobe epilepsy.

Epilepsy is a neurological disorder involving mitochondrial dysfunction and neuroinflammation. This study examines the relationship between NLRP3 inflammasome activation and mitophagy in the temporal lobe epilepsy, which has not been reported before. A pilocarpine-induced epileptic rat model was used to assess seizure activity and neuronal loss.

Climate Change and Topography Drive the Expansion of in the Alpine Treeline Ecotone of the Changbai Mountain.

Alpine treelines ecotones are critical ecological transition zones and are highly sensitive to global warming. However, the impact of climate on the distribution of treeline trees is not yet fully understood as this distribution may also be affected by other factors. Here, we used high-resolution satellite images with climatic and topographic variables to study changes in treeline tree distribution in the alpine treeline ecotone of the Changbai Mountain for the years 2002, 2010, 2017, and 2021.

Oncogenic Ras, Yki and Notch signals converge to confer clone competitiveness through Upd2.

Cell competition is an evolutionarily ancient mechanism that functions to remove unfit or dangerous clonal cells in a multicellular community. A classical model is the removal of polarity-deficient clones, such as the precancerous scribble (scrib) mutant clones, in Drosophila imaginal discs. The activation of Ras, Yki, or Notch signaling robustly reverses the scrib mutant clonal fate from elimination to tumorous growth.

Long-term efficacy of 10 kHz spinal cord stimulation in managing painful diabetic neuropathy: A post-study survey.

Objective: To evaluate the longer-term efficacy of 10 kHz spinal cord stimulation (SCS) in managing painful diabetic neuropathy (PDN) in a routine clinical setting after the transition from the 24-month SENZA-PDN study.

Methods: We contacted 142 participants who completed 24 months of postimplantation follow-up in the former randomized controlled trial (SENZA-PDN). Of these, 57 consented and responded to this longer-term post-study survey.

The risk perception and response of Azure-winged magpies: On the aspect of feeding behaviour and alarm calls.

Prey are expected to effectively perceive predation cues, recognise predators and adopt appropriate anti-predator strategies to enhance their chances of survival. Species with high cognitive abilities tend to be better at these processes, while empirical research is still lacking. The role of cognition in avoiding predation requires further investigation, especially in species with complex social structures and communication systems.

Bidirectional two-sample Mendelian randomization analysis identifies protein C rather than protein S or antithrombin-III as associated with deep venous thrombosis.

Introduction: Observational studies have indicated significant contributions of protein C and protein S to thrombotic diseases, yet the "anticoagulation paradox" in deep venous thrombosis (DVT) remains unresolved. Therefore, we conducted an investigation to discern the causal effects of protein C, protein S and antithrombin-III on DVT risk.

Material And Methods: We employed a two-sample (one to evaluate the gene-exposure relationship and the other to evaluate the gene-outcome relationship) bidirectional Mendelian randomization (MR) framework to assess the causal associations between protein C, protein S, antithrombin-III and DVT.

Single-cell spatial transcriptome atlas and whole-brain connectivity of the macaque claustrum.

Claustrum orchestrates brain functions via its connections with numerous brain regions, but its molecular and cellular organization remains unresolved. Single-nucleus RNA sequencing of 227,750 macaque claustral cells identified 48 transcriptome-defined cell types, with most glutamatergic neurons similar to deep-layer insular neurons. Comparison of macaque, marmoset, and mouse transcriptomes revealed macaque-specific cell types.