Upregulation of EMP3 in acute myeloid leukemia: a study based on data mining, RT-qPCR and immunohistochemistry.

Background: Epithelial Membrane Protein 3 (EMP3) has been associated with multiple malignancies, but its expression patterns and clinical significance in acute myeloid leukemia (AML) remain poorly characterized.

Methods: Public datasets were integrated to assess EMP3 mRNA expression levels in AML patients versus healthy donors, with validation performed using reverse transcription quantitative PCR (RT-qPCR). Protein expression was accessed through immunohistochemistry.

A Comparative Analysis of the Efficacy of Three Plant Growth Regulators and Dose Optimization for Improving Agronomic Traits and Seed Yield of Purple-Flowered Alfalfa ( L.).

This study evaluated the effects of different plant growth regulators and their concentration gradients on the agronomic traits, seed yield, and yield components of L. cv. "Xinmu No.

Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa.

Alfalfa ( L.) is a globally significant forage crop with notable economic value. Gibberellins (GA) promote dormancy breaking and early germination whereas auxins (IAA) predominantly influence bud regeneration.

Clinical validation and application of targeted long-range polymerase chain reaction and long-read sequencing-based analysis for hemophilia: experience from a hemophilia treatment center in China.

Background: Targeted long-read sequencing (LRS) is expected to comprehensively analyze diverse complex variants in hemophilia A (HA) and hemophilia B (HB) caused by the F8 and F9 genes, respectively. However, its clinical applicability still requires extensive validation.

Objectives: To evaluate the clinical applicability of targeted LRS-based analysis compared with routine polymerase chain reaction (PCR)-based methods.