Targeted Delivery of CD34 Aptamer-Coupled Tocilizumab Microspheres for Effective Treatment of Thyroid-Associated Ophthalmopathy.

Purpose: Thyroid-associated ophthalmopathy (TAO) is a debilitating autoimmune disorder linked to Graves' disease (GD) that is characterized by inflammation and remodeling of orbital tissues. This study focuses on the use of poly(lactic-co-glycolic) acid (PLGA)-based microspheres (MS) coupled with CD34-specific aptamers to enhance the targeted delivery of Tocilizumab (Toc), an IL-6 receptor monoclonal antibody, to CD34+ orbital fibroblasts, which is a critical cell type implicated in TAO.

Methods: The flow cytometry and aptamer-mediated pull-down assays were applied to detect the affinity of CD34 aptamers (Apts) for CD34+ orbital fibroblasts.

Quality of cataract-related videos on TikTok and its influencing factors: A cross-sectional study.

Objective: Short videos are increasingly being used to disseminate health information. However, the quality of videos on common ophthalmic conditions such as cataract has not been systematically evaluated.

Methods: This study employed a cross-sectional design.

A bibliometric and visual analysis of Jak1 to explore trends and frontiers.

Background: JAK1, a member of the JAK kinase family, is involved in the signal transduction of multiple cytokine pathways and is crucial in the onset and progression of inflammation and tumours. Consequently, JAK1 has garnered significant attention in recent years.

Methods: We use bibliometric and visual analysis to evaluate the thematic trends and knowledge structure of TRPV1's research papers on JAK1, sourced from the Web of Science core collection from 2003 to 2024.

The miR-320a/PRDX3 Axis Alleviates the Oxidative Stress and Fibrotic Alterations in Fibroblasts in Thyroid Eye Disease.

Purpose: Thyroid eye disease (TED) is an autoimmune condition of the orbit characterized by significant morbidity. MicroRNAs (miRNAs) have been identified as key modulators of immune responses and fibrotic processes, with miR-320a emerging as a potential modulator in TED pathogenesis.

Methods: This study investigated miR-320a's role in TED by analyzing its expression in TED tissues and assessing the impact of its inhibition on fibroblast activation, fibrosis, and oxidative stress.

Quality and content evaluation of thyroid eye disease treatment information on TikTok and Bilibili.

Thyroid eye disease (TED) is an autoimmune condition that commonly impacts patients' visual function, appearance, and psychological well-being. Challenges in TED management include low early detection rates and large variation in treatment response. Video platforms like TikTok and Bilibili are increasingly utilized for health information dissemination, yet the quality of TED treatment content on these platforms varies significantly.

[One new sesquiterpene from Aquilariae Lignum Resinatum].

The chemical constituents of sesquiterpenes from 95% ethanol extract of Aquilariae Lignum Resinatum were isolated and purified by various column chromatography techniques, including silica gel, Sephadex LH-20, octadecylsilyl(ODS), and semi-preparative high performance liquid chromatography(HPLC). Their planar structures and absolute configurations were elucidated by ultraviolet(UV) spectrometry, infrared(IR) spectroscopy, mass spectrometry(MS), nuclear magnetic resonance(NMR), electronic circular dichroism(ECD), and other techniques. Eight sesquiterpenoids were isolated and identified as(+)-(7R,10R)-selina-4,11-dien-12-dimethoxy-15-al(1),(+)-(7R,10R)-selina-4,11-diene-12,15-dial(2), agalleudesmanol B(3), aquisinenoid C(4), 12,15-dioxo-α-selinen(5), agarospiranic aldehyde B(6), neopetasane(7), and eremophila-7(11),9-dien-8-one(8).

Integrin αvβ6 mediates the effects of age-related decreases in matrix stiffness on Tenon's fibroblast activation and bleb scarring in glaucoma.

Glaucoma, including primary open-angle glaucoma (POAG) and primary angle-closure glaucoma, leads to optic nerve injury and visual field loss, often necessitating surgical intervention to lower intraocular pressure (IOP). Trabeculectomy, the most common glaucoma surgery, could fail due to excessive scarring of the filtering bleb, driven by the hyperproliferation of human Tenon's fibroblasts (HTFs). Herein, the impact of aging on matrix stiffness in Tenon's capsule tissue, the role of extracellular matrix (ECM) stiffness in the phenotypic transformation of HTFs, and the regulatory function of integrin alphavbeta6 (αvβ6) were investigated.

Sleep quality and its determinants in patients with thyroid eye disease: a cross-sectional study.

Background: Sleep disorders may impair the nervous and immune systems. In thyroid eye disease (TED), an autoimmune condition, sleep quality and its potential determinants remain unclear. The study aimed to assess the sleep quality of patients with TED and identify its determinants.

Development and validation of a predictive model for depression risk in Chinese obese adults.

Objective: To construct a prediction model for the risk of depression in the obese population, aiming to facilitate the early identification of high-risk individuals and guide personalized preventive interventions.

Methods: This study was based on the data from the China Health and Retirement Longitudinal Study (CHARLS 2015), the Center for Epidemiologic Studies Depression Scale-10 (CES-D10) to assess the depression of obese patients, Lasso regression and multivariable logistic regression were used to select predictors, the construction of a nomogram model, and the use of the random splitting method divided into a training set ( = 974) and a validation set ( = 418) by the 7:3 method, and the model was evaluated by the ROC curves and the AUC, the H-L goodness-of-fit test, the calibration graphs, and the clinical decision-making curve to assess the model.

Results: A total of 1,392 obese patients were finally included, with a prevalence of depression of 32.

Correlation Between Macular Microstructural Changes with Disease Staging and Visual Acuity in Diabetic Retinopathy.

Purpose: To investigate the changes in macular microvascular structure at different stages of diabetic retinopathy (DR) and the correlation between macular ischemia and visual acuity.

Patients And Methods: A prospective cross-sectional study was conducted. A total of 173 patients with DR were enrolled and divided into three groups according to DR stage.

3-Oxo-22α-Hydroxy-Rotundic Acid Alleviates Hyperlipidemia in Mice by Modulating Lipid Metabolism Through the AMPK-SREBP-1c-PPARα Pathway.

3-Oxo-22α-hydroxy-rotundic acid (ITP3) demonstrated notable hypolipidemic activity. However, the molecular mechanism of its hypolipidemic activity has not been elucidated. The present study aimed to evaluate its lipid-lowering efficacy using in vivo and in vitro hyperlipidemia models and to further elucidate its potential mechanism of action in hyperlipidemia.

Phenylpropanoid Metabolites Mediate Antiviral Defense and Vector Resistance in Rice Infected With RRSV, RGSV, and SRBSDV.

Rice viruses frequently cause major yield losses, threatening global food security. In this study, we employed a multi-omics approach (transcriptome, small RNA and metabolome) to investigate the common and distinct responses of rice to three dwarfing viruses, rice ragged stunt virus, rice grassy stunt virus and Southern rice black-streaked dwarf virus. All three pathogens elicited notable dwarfing and increased tillering in infected plants, which significantly impacted yield potential.

A new limonoid from the fruits of var. (Siebold ex Hoola van Nooten) Swingle.

A new limonoid named ethyl nomilinate () and 10 known limonoids () were isolated from the fruits of var. (Siebold ex Hoola van Nooten) Swingle. Their structures were elucidated by HRESIMS and NMR analyses.

MsbZIP55 regulates salinity tolerance by modulating melatonin biosynthesis in alfalfa.

Soil salinity is a severe abiotic stress that damages plant growth and development. As an antioxidant and free radical scavenger, melatonin is well known for helping plants survive abiotic conditions, including salinity stress. Here, we report that the salt-related gene MsSNAT1, encoding a rate-limiting melatonin biosynthesis enzyme, is located in the chloroplast and contributes to salinity stress tolerance in alfalfa.

Butyrate Ameliorates Graves' Orbitopathy Through Regulating Orbital Fibroblast Phenotypes and Gut Microbiota.

Purpose: Graves' orbitopathy (GO), the common extrathyroidal complication of Graves' disease (GD), is characterized by orbital fibroblast stimulation, adipogenesis, and hyaluronan production. Recently, gut microbiota and its metabolites have garnered attention for their possible involvement in GO.

Methods: This study utilized an animal model of GO and examined the effects of butyrate treatment on orbital fibroblast cells and gut microbiota.

Triterpenoids from ilicis rotundae cortex ameliorate hyperlipidemia by affecting bile acids-hepatointestinal FXR axis.

Background: Hyperlipidemia is a lipid metabolism disorder that, in severe cases, can lead to conditions such as hypertension, coronary heart disease, and cirrhosis. Previous studies have identified Ilicis Rotundae Cortex (IRC) crude extract as having the potential to regulate blood lipids. However, whether the triterpenoids therein are the principal agents responsible for hypolipidemic effects and their specific mechanisms of action remain unexplored.

Development of an advanced acetaldehyde detection solution based on yeast and bacterial surface display technology.

Acetaldehyde, a carcinogen widely present in various beverages and the natural environment, necessitates convenient and efficient detection methods. In this work, two different host strains were used to develop a sensitive, convenient, and efficient whole-cell optical biosensor for acetaldehyde detection. Acetaldehyde dehydrogenase (AldH) was displayed on the cell surface of Saccharomyces cerevisiae and E.

Traditional Chinese medicine FYTF-919 (Zhongfeng Xingnao oral prescription) for the treatment of acute intracerebral haemorrhage: a multicentre, randomised, placebo-controlled, double-blind, clinical trial.

Background: There are few proven treatments for acute spontaneous intracerebral haemorrhage, and they all target reducing expansion of the haematoma. The traditional Chinese medicine FYTF-919 (Zhongfeng Xingnao) in an oral solution is comprised of several Chinese herbs that are widely used to treat patients with intracerebral haemorrhage in China on the understanding that they enhance resorption of the haematoma and reduce neuroinflammation. We aimed to provide a reliable assessment of the safety and efficacy of FYTF-919 in patients with moderate to severe acute intracerebral haemorrhage.

Orbital decompression improves visual function and macular blood perfusion status in patients with thyroid-related eye disease.

Background: Orbital decompression surgery is a treatment option for Thyroid-associated Ophthalmopathy (TAO). However, its effects on visual function and macular perfusion status remain unclear. This study examined how orbital decompression surgery affects visual acuity and macular blood flow in TAO patients and the variation between these two factors.

Correlation between macular perfusion status and visual acuity in retinal vein occlusion.

Objectives: Retinal vein occlusion (RVO) is the second most common retinal vascular disease worldwide, and the retinal perfusion status is closely related to the prognosis of the disease. Macular perfusion status is particularly correlated with visual acuity. This study aims to investigate the changes in macular perfusion indicators in RVO using optical coherence tomography angiography (OCTA) and analyze the correlation between macular perfusion status and visual acuity.

Significance of Gut Microbiota on Graves' Disease.

Growing research proves gut microbiota and thyroid autoimmunity are linked. Graves' disease (GD), as an autoimmune thyroid disease (AITD), is attributed to the production of thyroid-stimulating hormone receptor (TSHR) autoantibodies that bind to the thyroid follicular endothelial cells. It is well known that genetic factors, environmental factors, and immune disorders count for much in the development of GD.

Elucidating the role of genetically determined metabolites in Diabetic Retinopathy: insights from a mendelian randomization analysis.

Aims: Diabetic retinopathy (DR) results from complex genetic and metabolic interactions. Unraveling the links between blood metabolites and DR can advance risk prediction and therapy.

Methods: Leveraging Mendelian Randomization (MR) and Linkage Disequilibrium Score Regression (LDSC), we analyzed 10,413 DR cases and 308,633 controls.