Lumbrokinase-loaded GelMA hydrogels with inflammatory regulatory capacity promote vascularized bone regeneration in critical-sized cranial defects.

The management of critical-sized bone defects resulting from trauma and resective surgeries still poses significant clinical challenges. Factors contributing to inadequate bone regeneration in critical-sized defects are complex and multifaceted, including vascular injury and inflammation. Inspired by earthworms' remarkable regenerative abilities, we incorporated lumbrokinase (LK), an extract from earthworms, into injectable gelatin methacryloyl (GelMA) hydrogels to achieve controlled release of LK, thereby enhancing vascularized bone regeneration in critical-sized defects.

Expert consensus on the diagnosis and treatment of cemental tear.

Cemental tear is a rare and indetectable condition unless obvious clinical signs present with the involvement of surrounding periodontal and periapical tissues. Due to its clinical manifestations similar to common dental issues, such as vertical root fracture, primary endodontic diseases, and periodontal diseases, as well as the low awareness of cemental tear for clinicians, misdiagnosis often occurs. The critical principle for cemental tear treatment is to remove torn fragments, and overlooking fragments leads to futile therapy, which could deteriorate the conditions of the affected teeth.

Occlusal disharmony attenuates inhibitory synaptic transmission in the medial prefrontal cortex and contributes to anxiety.

Background: It has been well-validated that Occlusal Disharmony (OD) induces negative emotions, especially anxiety. While it leads to considerable frustration for both doctors and patients, its underlying mechanisms remain unclear, and effective treatment has been lacking. The present study aims to explore the pathological mechanisms of OD induced anxiety and to find a straightforward yet effective treatment strategy for this ostensibly intricate clinical phenomenon.

Current status and future direction of mechanotransduction mechanisms: Perspectives from cell membrane to nucleus.

Mechanical stimuli are widely present in both in vivo and in vitro environments, and studies have shown that these mechanical stimuli can significantly modulate cell fate and regulate physiological or pathological processes such as embryonic development and tumor metastasis. Cells have abundant biomechanical signaling pathways, which can be mainly divided into three types of pathways: cell membrane (integrins, ion channels), cytoplasm (cytoskeleton), and nucleus (nuclear pore complexes, chromatin epigenetic alterations). Through the above-mentioned outside-in and inside-out signaling axes, the cells can accurately perceive the mechanical changes in the microenvironment and make adaptive changes.

deteriorates autism spectrum disorders by disturbing the gut and oral microbiota.

Autism spectrum disorder (ASD) significantly impairs socialization and communication, posing a major societal challenge due to limited understanding of its pathogenesis and lack of effective treatments. Recent studies have shown an imbalance in the oral and intestinal microbiota of individuals with ASD, which may exacerbate ASD symptoms. In this study, we successfully established an ASD mouse model induced by () solution.

LPS-Induced Mitochondrial Damage via SLC41A1-Mediated Magnesium Ion Efflux Leads to the Pyroptosis of Dental Stem Cells.

Although regenerative endodontics demonstrate promise for dental pulp regeneration, chronic inflammation often hinders the success. This study aims to explore the mechanism whereby lipopolysaccharide (LPS) induces dental pulp regeneration failure. Transcriptomic profiling of LPS-stimulated dental pulp stem cells (DPSCs) reveals dysregulated cation homeostasis and increased magnesium (Mg⁺) transmembrane transport.

Snhg18 regulates Yap subcellular localization to maintain bone homeostasis.

Osteoporosis treatments commonly mitigate bone loss but rarely restore lost bone mass. Yes-associated protein (Yap) nuclear translocation is crucial for the osteogenic differentiation of bone marrow stromal cells (BMSCs), but is disrupted by many factors under osteoporotic conditions. Long non-coding RNAs (lncRNAs) regulate BMSCs differentiation and Yap localization across diseases, exhibiting tissue- and cell-specific effects.

Neutrophil extracellular traps aggravate periodontitis by disturbing regulatory T-cell differentiation.

Excessive neutrophil extracellular traps (NETs) induce an intense inflammatory response in periodontitis. Recently, Tregs were shown to be essential for attenuating inflammation-driven bone resorption. However, the regulation of Tregs differentiation by NETs in periodontitis is still unclear and needs further investigation.

Development of health emergency response capability evaluation framework for primary health institutions in metropolis: based on Delphi method and analytic hierarchy process.

Background: Primary health institutions, as the "first on-site responders," play a crucial role in responding to health emergencies. However, there are few studies on the systematic assessment of their emergency response capabilities. We aimed to develop a health emergency response capability evaluation framework for primary health institutions to assess the resuscitation capacity in metropolis.

Hierarchical Nanocellulose-Based Functional Films: A Sustainable and High-Performance Alternative to Conventional Plastic Films.

The rapid increase in plastic production has led to significant environmental challenges due to the accumulation of nondegradable plastic waste, underscoring the urgent need for sustainable alternatives. Nanocellulose, the most abundant renewable biopolymer on Earth, has emerged as a promising candidate for next-generation film materials. This review explores the potential of nanocellulose films as a sustainable replacement for conventional oil-derived plastic films, focusing on its hierarchical structure, tunable properties, and diverse functionalities.

Expert consensus on digital restoration of complete dentures.

Digital technologies have become an integral part of complete denture restoration. With advancement in computer-aided design and computer-aided manufacturing (CAD/CAM), tools such as intraoral scanning, facial scanning, 3D printing, and numerical control machining are reshaping the workflow of complete denture restoration. Unlike conventional methods that rely heavily on clinical experience and manual techniques, digital technologies offer greater precision, predictability, and efficacy.

Parasubthalamic Glutamatergic Neurons Coordinate Cardiovascular Homeostasis and Locomotion in Mice.

Cardiovascular homeostasis and locomotion are vital for animal survival and the maintenance of essential physiological functions. The parasubthalamic nucleus (PSTN) has recently emerged as a novel interoceptive center in the central nervous system, yet its role in coordinating cardiovascular homeostasis and locomotor behavior remains poorly understood. Here, we demonstrate that PSTN neurons serve as a novel baroreflex center, exhibiting heightened activity during acute hypertension.

An optical model for evaluating the influence of microstructures on zirconia translucency.

Objectives: Pores and tetragonal phase crystallites are the two main scatterers in yttria-stabilized zirconia (YSZ) ceramics. Previous optical models have often overestimated the proportion of the tetragonal phase and lacked accurate characterization of pore distribution. The aim of this study was therefore to construct a transmission-scattering model for zirconia, to separately calculate and analyze the contributions of pores and tetragonal phase crystallites to translucency.

Procr chondroprogenitors sense mechanical stimuli to govern articular cartilage maintenance and regeneration.

Protein C receptor (Procr) cells were identified as stem or progenitor cells in multiple adult tissues. However, whether mechanical stimuli fine-tune their activation and differentiation remain unknown. Here, we found rare Procr cells in the superficial layer of tibial articular cartilage and meniscus, which keep replenishing chondrocytes in postnatal knee joints.

ASXL2 Modulates Chromatin Remodeling to Direct Osteogenesis and Multiple Cell Fate in hPDLSCs.

Aims: This study investigates the epigenetic role of Additional Sex Combs-Like 2 (ASXL2) in regulating osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs), aiming to address limitations in current strategies for oral and maxillofacial tissue regeneration.

Methods: Lentiviral-mediated ASXL2 knockdown in hPDLSCs was validated by flow cytometry. Functional impacts were assessed through proliferation/apoptosis assays, alkaline phosphatase (ALP) activity, Alizarin Red mineralization, and qPCR/Western blot analyses of osteogenic markers (RUNX2, ALP, COL1A1, OCN).

The Effects of Azithromycin Modified Triple Antibiotic Paste in Resolving Periapical Inflammation.

Objective: This study aimed to assess the anti-inflammatory and antibacterial properties of traditional and modified triple antibiotic paste. The 16S rDNA was employed to analyse the root canal microbial community's characteristics pre- and post-treatment.

Methods: Rabbits with periapical inflammation (AP) were treated with both pastes.

Expert consensus on the prevention and treatment of radiochemotherapy-induced oral mucositis.

Radiochemotherapy-induced oral mucositis (OM) is a common oral complication in patients with tumors following head and neck radiotherapy or chemotherapy. Erosion and ulcers are the main features of OM that seriously affect the quality of life of patients and even the progress of tumor treatment. To date, differences in clinical prevention and treatment plans for OM have been noted among doctors of various specialties, which has increased the uncertainty of treatment effects.

Dual-functionalized graphene oxide induces M2a and M2c macrophage polarization to orchestrate inflammation and tissue remodeling.

Functional tissue repair is often constrained by inflammation and fibrosis. Alternatively activated M2 macrophages have emerged as promising therapeutic targets for optimizing graft-to-host interactions; however, efficient induction methods are required. Presumably, the outcome of regenerative wound healing or scar formation/fibrosis might be dependent on the balance between M2a and M2c sub-phenotypes.

Tensile stress promotes the chondrogenic ability of condylar cartilage stem/progenitor cells in the temporomandibular joint via the Piezo1-Ca-Prkca pathway.

Background: Tensile force is a key regulator for condylar cartilage remodeling in the temporomandibular joint (TMJ), and this biomechanical characteristic underlies the mechanisms of mandibular growth modification in orthodontic practice. Cartilage stem/progenitor cells (CSPCs) in the superficial layer of condylar cartilage play an essential part in the development and remodeling of condylar cartilage. However, the regulatory role of tensile force on condylar CSPCs remains unclear.

Exploring the Correlation Between Psychological Stress, Anxiety, and Periodontitis Among University Students: A Cross-Sectional Investigation.

Objective: Periodontitis is a prevalent chronic inflammatory disease, with growing evidence suggesting a link to psychological factors such as stress and anxiety. University students, who frequently experience elevated psychological stress, may be particularly susceptible to periodontal issues. This study aimed to examine the relationship between stress levels, anxiety status, and periodontitis in university students, and to assess the potential influence of psychological factors on periodontal health.

Influence of abutment types on the success of two-unit implant-supported fixed dental prostheses: A three-dimensional finite element analysis and fatigue test combined with microleakage evaluation.

Statement Of Problem: Implant-supported splinted prostheses have been widely used to rehabilitate partial edentulism. However, few studies have investigated how different abutment types affect stress distribution in dental implants and the surrounding bone structures of splinted crowns. In addition, which combination of abutments helps reduce microleakage at the abutment-implant interface the most is unclear.

The Joint Impact of Heavy Metals and Polycyclic Aromatic Hydrocarbons on Periodontitis.

Objective: Heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) are significant environmental pollutants, raising growing concerns about their potential impact on human health, particularly oral health. This study aimed to investigate the combined effects of these pollutants on periodontitis (PD) - a prevalent chronic inflammatory disease affecting tooth-supporting structures.

Methods: This study utilized the National Health and Nutrition Examination Survey data to examine interactions between HMs, PAHs, and PD.

Sword and Board in One: A Bioinspired Nanocomposite Membrane for Guided Bone Regeneration.

Guided bone regeneration (GBR) faces far from a one-dimensional challenge. It demands a multifunctional membrane to possess paradoxical but essential properties in sophisticated clinical scenarios. Drawing inspiration from natural biological structures and superior properties, through a combination of structure innovation and composition regulation, a multicomponent nacre-inspired discontinuous Bouligand structure is devised and transcribed into a bioinspired Janus nanocomposite membrane with comprehensive properties for GBR, identical to "sword and board.

ER-induced PERK/TFEB cascade sequentially modulates mitochondrial dynamics during cranial suture expansion.

The effectiveness of cranial suture expansion therapy hinges on the timely and adequate regeneration of bone tissue in response to mechanical stimuli. To optimize clinical outcomes and prevent post-expansion relapse, we delved into the underlying mechanisms governing bone remodeling during the processes of suture expansion and relapse. Our findings revealed that in vitro stretching bolstered mesenchymal stem cells' antioxidative and osteogenic capacity by orchestrating mitochondrial activities, which governed by force-induced endoplasmic reticulum (ER) stress.