Inhibition of Interleukin-1 Receptor-Associated Kinase-1 by Pacritinib Is a Therapeutic Strategy to Overcome Melanoma Resistance.

Despite advancements in systemic therapy, the mortality rate for patients with metastatic melanoma remains around 70%, underscoring the imperative for alternative treatment strategies. Through the establishment of a chemoresistant melanoma model and a subsequent drug investigation, we have identified pacritinib, a medication designed for treating myelofibrosis and severe thrombocytopenia, as a potential candidate to overcome resistance to melanoma therapy. Our research reveals that pacritinib, administered at clinically achievable concentrations, effectively targets dacarbazine-resistant melanoma cells by suppressing IRAK1 rather than JAK2.

Recent progress of rapid detection technologies for mycotoxins.

Mycotoxins, due to their high carcinogenic and genotoxic properties, pose a significant threat to global food safety. Traditional detection methods often fall short in meeting the demands for large-scale, real-time, simple, and rapid monitoring. As a result, innovative rapid detection approaches, leveraging advanced materials and sensor technologies, are emerging as key solutions for preventing food contamination.

Starch crystalline types modulate myofibrillar protein aggregation and its functional properties in gelling and emulsifying systems.

Different starch crystal structures significantly influence meat product quality, though their specific impacts on myofibrillar protein (MP) functionality remain unclear despite industry demand for optimized ingredients. This study compared how potato, corn, mung bean, and pea starches affect MP properties in minced pork. Our findings reveal that starch-protein interactions fundamentally regulate MP gel and emulsion properties through the following mechanisms: First, starch promotes protein aggregation by enhancing hydrophobic interactions and disulfide bond formation, affecting gel network crosslinking.

Targeted degradation of Werner syndrome helicase (WRN) via ligand-directed covalent hydrophobic tagging.

The Werner syndrome RecQ helicase (WRN) has recently emerged as a novel synthetic lethality target for microsatellite instability-high (MSI-H) cancers. However, available WRN inhibitors or degraders is still lacking so far. Particularly, chemically designed probes capable of degrading WRN irrespective of microsatellite status remain unexplored.

Design and Fabrication of Flexible Silk Fibroin/Lanthanide Ion Membranes with Multifunctional Properties of Fluorescence, Humidity Sensitivity, and Conductivity.

Silk fibroin (SF)-based flexible electronic/photonic materials have gained great attention in wearable devices and soft sensors. However, it remains challenging to understand the molecular interaction mechanisms and subsequently fabricate SF-based flexible materials that exhibit fluorescence, humidity sensitivity, and conductivity properties. In this study, by incorporating lanthanide europium ion (Eu), the design and fabrication of a flexible, fluorescent, and conductive SF membrane was proposed.

Preparation and Characterization of Polysaccharides From Grifola frondosa and Their Human Intestinal Flora-modulating Effect.

A novel and efficient hydrogen peroxide/ascorbic acid-assisted extraction method for the preparation of Grifola frondosa polysaccharide (GFP) was developed, and two GFP fractions (GFP-H and GFP-L) with different molecular weights (Mws) were obtained by separation with ultrafiltration. Both high Mw component (GFP-H, Mw 396.4 kDa) and low Mw component (GFP-L, Mw 12.

Peptide Sequence Modulating the Analytical Performance of Electrogenerated Chemiluminescence Peptide-Based Biosensors for Matrix Metalloproteinase 2.

Electrogenerated chemiluminescence (ECL) methods have been widely used in clinical diagnosis. Although ECL peptide-based biosensors continue to grow with good sensitivity and signal flexibility, little emphasis has been placed on the effect of the peptide sequence on ECL sensitivity. We herein studied the nuanced effects of different peptide sequences on the analytical performance of ECL peptide-based biosensors for matrix metalloproteinase 2 (MMP-2) assay, in which [(pbz)Ir(DMSO)Cl] (pbz = 3-(2-pyridyl)benzoic acid) was used as the ECL emitter while a specific peptide was used as the molecular recognition element.

Ligand Engineering-Driven Visualization of Asymmetric Exchange: Unraveling the Structural Evolution of Ag to Ag.

Precisely structured nanoclusters provide ideal platforms for elucidating structural evolution and structure-activity relationships. However, mechanistic understanding of dynamic core-shell rearrangements has long been impeded by the elusive nature of intermediates during transformation processes. Here, we show that ligand engineering-driven asymmetric thiolate exchange enables atomic-level visualization of structural evolution, thereby overcoming the long-standing challenge of intermediate capture.

Topology in Thermal, Particle, and Plasma Diffusion Metamaterials.

Diffusion is a fundamental process in the transfer of mass and energy. Diffusion metamaterials, a class of engineered materials with distinctive properties, enable precise control and manipulation of diffusion processes. Meanwhile, topology, a branch of mathematics, has attracted growing interest within the condensed matter physics community.

Engineering of Core-Shell Pd/SSZ-13@AlO Zeolite: Unlocking Superior NO Adsorption and Chemical Durability.

Pd-zeolites are promising passive NO adsorber (PNA) materials for mitigating cold-start emissions from lean-burn engines. However, their practical deployment is constrained by insufficient densities and dispersion of isolated Pd active sites as well as their susceptibility to hydrothermal degradation and phosphorus poisoning encountered in vehicle exhaust environments. Herein, we develop a rationally engineered core-shell Pd/SSZ-13@AlO composite, featuring a Pd/SSZ-13 core encapsulated within a mesoporous AlO shell.

Tailoring Active Sites in Amorphous NiFe-MOFs through Pyridine Ligand Coordination for Enhanced Oxygen Evolution Performance.

The development of high-performance, cost-effective non-noble metal catalysts for the oxygen evolution reaction (OER) is critical to advancing sustainable hydrogen production via water electrolysis. Herein, we report a facile and mild strategy for synthesizing amorphous bimetallic organic framework materials (NiFe-MOFs) using pyridine-modified threonine (l-PyThr) as an organic ligand. The optimized NiFe-PyThr-4:1 catalyst exhibits remarkable OER activity, requiring low overpotentials of only 162 and 222 mV to achieve current densities of 10 and 100 mA cm, respectively, along with a small Tafel slope of 34.

Information sharing and channel structure in e-commerce supply chain considering data-driven marketing.

E-tailers such as Amazon and Tmall can accurately recognize consumer interest in product categories and grasp current consumer trends through product recommendation algorithms and data-driven analysis. In this study, we develop a game-theoretic model to investigate the encroachment and information sharing decisions considering data-driven marketing (DDM). Our outcomes reveal that the manufacturer has the incentive to reduce the wholesale price to incentivize the e-tailer to increase the DDM effort when the spillover effect is high and the marginal cost is low.

Fragment dispersity index analysis of cfDNA fragments reveals chromatin accessibility and enables early cancer detection.

We introduce a cell-free DNA (cfDNA) fragmentation pattern: the fragment dispersity index (FDI), which integrates information on the distribution of cfDNA fragment ends with the variation in fragment coverage, enabling precise characterization of chromatin accessibility in specific regions. The FDI shows a strong correlation with chromatin accessibility and gene expression, and regions with high FDI are enriched in active regulatory elements. Using whole-genome cfDNA data from five datasets, we developed and validated the FDI-oncology model, which demonstrates robust performance in early cancer diagnosis, subtyping, and prognosis.

EzBioCloud 16S rRNA Gene Sequence Formatter: a Python-based sequence formatting tool for systematic microbiology.

EzBioCloud is one of the practical reference databases and analytical platforms for systematic microbiology research. The EzBioCloud database provides convenient services in this regard, especially for performing sequence analysis using the 16S rRNA genes. However, '.

Diverse biofilm-forming represent twelve novel species isolated from glaciers on the Tibetan Plateau.

The family , encompassing the genus and related taxa, comprises diverse Gram-negative, aerobic, rod-shaped bacteria found in varied habitats, including air, soil, water and glaciers. Recent genomic-based taxonomic revisions have reclassified some species into new genera, such as and , due to polyphyletic relationships within the family . Certain species are known for forming biofilms or functioning as aerobic anoxygenic phototrophic bacteria, traits that enhance resilience in extreme environments like the cryosphere.

sp. nov., isolated from the upper respiratory tract of duck.

Five bacterial strains, designated as RCAD1438, RCAD1439, RCAD1670, RCAD1671 and RCAD1672, were isolated from the upper respiratory tract of ducks in Anhui, Shaanxi and Sichuan, China. All strains are Gram-stain-negative, rod-shaped, non-motile, non-spore-forming, aerobic and capsulated. They grow optimally at 37 °C and pH 7.

Polystyrene Nanoplastics Regulate Silicon Cycling and Biosilica Deposition in Marine .

Nanoplastics are emerging pollutants with the potential to disrupt the microbial physiology and biogeochemical cycles in marine ecosystems. However, their influence on silicon cycling in cyanobacteria remains poorly understood. Here, we investigate how amine-modified polystyrene nanoplastics (PS-NH) regulate silicon transport and biosilica deposition in sp.

P-Doped Cu-N-C Single-Atom Catalysts Boost Cathodic Electrochemiluminescence of Luminol for MicroRNA-320d Detection.

Compared with efficient anodic luminol electrochemiluminescence (ECL), the disadvantage of cathodic ECL is that luminol cannot be electrochemically oxidized in a direct manner, and the conversion efficiency of dissolved oxygen (DO) as the coreactant to reactive oxygen species (ROS) is poor, which limits its application. Therefore, it is necessary to develop a functional catalyst suitable for the luminol-DO ECL system to directly trigger cathodic ECL. In this study, a coordination microenvironment modulation strategy was proposed.

Light-Promoted C(sp)-H Functionalization: Alkyl Amines as C2 Synthons in Formal [3+2] Cycloaddition with -Imino(iso)quinolinium Ylides.

The classical 1,3-dipolar cycloaddition reaction predominantly generates five-membered heterocycles through cycloaddition between 1,3-dipoles and unsaturated hydrocarbons, such as alkenes or alkynes. Herein, we report a light-mediated approach that employs saturated amines as alternatives to conventional unsaturated hydrocarbons, enabling their reaction with -imino(iso)quinolinium ylides to achieve efficient synthesis of pyrazolo-fused (iso)quinoline core structures. This method demonstrates operational simplicity, mild reaction conditions, and excellent functional group compatibility.

Cellulosic Flexible Electronic Materials: Recent Advances in Structural Design, Functionalization, and Smart Applications.

Rapid advancement of flexible electronics has generated a demand for sustainable materials. Cellulose, a renewable biopolymer, exhibits exceptional mechanical strength, customizable properties, biodegradability, and biocompatibility. These attributes are largely due to its hierarchical nanostructures and modifiable surface chemistry.

Electron-Rich Macrocycle-Based Metal-Organic Frameworks for Efficient Photocatalytic CO Reduction.

Metal-organic frameworks (MOFs) are distinguished by their structural diversity, tunable electronic properties, and exceptional performance in various applications. Notably, the electron-donating ability of ligands significantly enhances the ligand-to-metal charge transfer (LMCT) processes within these frameworks, thereby promoting efficient charge migration. Herein, we developed two electron-rich macrocyclic ligands derived from phenothiazine- and phenoxazine-functionalized calix[3]arenes, alongside their corresponding cobalt-coordinated MOFs.

Stretchable Sensor-Integrated Microfluidic Chip for Monitoring Fatty Acid-Enhanced Serotonin Release from the Intestinal Epithelium.

High-fat foods are decomposed into fatty acids during digestion and absorption, primarily occurring in the gastrointestinal tract, and numerous studies have indicated that long-term high-fat diets significantly increase the incidence of intestinal disorders. As a critical intestinal hormone, serotonin (5-hydroxytryptamine, 5-HT) is involved in regulating intestinal peristalsis, secretion, and visceral sensitivity. However, due to the lack of methods capable of reproducing intestinal mechanical activities and in situ monitoring of 5-HT levels, the influence of high-fat diets on intestinal 5-HT release remains unclear.

Association between the uric acid to high-density lipoprotein ratio (UHR) and hypertension in US adults: evidence from NHANES 2005-2020.

Uric acid to HDL ratio (UHR) is a new measure of inflammation that has been widely used to study cardiovascular disease relationships. The aim of this study was to investigate the relationship between uric acid to HDL ratio and hypertension. We found that UHR was positively associated with hypertension prevalence in a nationally representative sample of U.

Unveiling Ion-Transport Dynamics in 2D Nanofluidic Anion-Selective Membranes toward Osmotic Energy Harvesting.

Two-dimensional (2D) nanofluidic architectures with nanoconfined interlayer channels and excess surface charges have revolutionized membrane-based reverse electrodialysis systems, demonstrating highly efficient osmotic energy collection through strong electrostatic screening of electric double layer (EDL). However, the ion-transport dynamics in 2D nanofluidic anion-selective membranes (2D-NAMs) still remain unexplored. Here, we combine density functional theory and molecular dynamics (MD) simulations to systematically explore ion transport in the 2D-NAMs.

G20 stroke burden 1990-2035: trends, projections and precision health insights.

Background: Stroke persists as the second leading global cause of mortality and disability. We analyzed G20 nations using Global Burden of Disease (GBD) 2021 data (1990-2021) to provide a new perspective.

Methods: We obtained age-standardized rates (ASR) of stroke mortality, incidence, prevalence, and YLLs (years of life lost) across G20 nations.