Development of the CRISPR/Cas9 system for genome editing in Riemerella anatipestifer.

Riemerella anatipestifer (R. anatipestifer) is an important pathogen responsible for high mortality rates and severe economic losses in the poultry industry. Research on R.

Emerging zoonotic pathogen Staphylococcus borealis mediates longitudinal increase and environmental contamination of tigecycline resistance throughout the pig production period.

The widespread use of tetracyclines in swine production has accelerated the emergence of tigecycline-resistant Staphylococcus, posing a threat to public health. This study investigated the prevalence and mechanisms of tigecycline resistance in pigs, where tigecycline is unapproved yet resistance is present. The emerging zoonotic pathogen Staphylococcus borealis played an important role.

Identification and characterization of short-chain dehydrogenase/reductase 3 (DHRS3) deficiency, a retinoic acid embryopathy of humans.

Purpose: Signaling by the morphogen all-trans retinoic acid (RA) is critical for embryonic development, during which its tissue concentration must be tightly regulated. We investigated 8 sibships (12 individuals) segregating 5 different homozygous variants of dehydrogenase/reductase 3 ), which encodes an embryonically expressed enzyme (short-chain dehydrogenase/reductase 3; also termed SDR16C1) that catalyzes the reduction of retinaldehyde to retinol, limiting excessive RA synthesis.

Methods: We assessed variant pathogenicity using comparative phenotypic and bioinformatic analysis, quantification of expression, and measurement of plasma retinoid metabolites.

The bidirectional regulatory effect of TXNRD2 methylation in patients with chronic heart failure and its nonlinear dose-response relationship with key clinical parameters.

Objective: The research focused on examining CpG methylation within the promoter area in chronic heart failure (CHF) patients, aiming to correlate methylation levels with clinical indexes to guide CHF treatment.

Methods: Whole blood samples from 20 CHF patients and 20 healthy controls were analyzed using MALDI-TOF-MS. Methylation levels of CpGs in the -FA42 region were compared between CHF patients, healthy controls, and CHF patients with varying cardiac functions.

Identification and measurement of potential land use conflicts in "production-living-ecological" overlapping spaces of Chang'an District, Xi'an City, China.

Under the backgrounds of urbanization expansion, agricultural development transformation, and the steady advancement of ecological civilization construction, identifying and measuring land use conflicts in production-living-ecological overlapping spaces is crucial for the coordinated development of national land space. We integrated two potential land use conflicts identification methods: production-living-ecological functional characterization and multi-scenario simulation, incorporated ecological risk theory to develop a research framework based on scenario simulation-potential conflicts identification-potential conflicts measurement. We identified and measured potential land use conflicts in three scenarios for Chang'an District of Xi'an in 2030.

Cell extrusion drives neural crest cell delamination.

Neural crest cells (NCC) comprise a heterogeneous population of cells with variable potency that contribute to nearly every tissue and organ throughout the body. Considered unique to vertebrates, NCC are transiently generated within the dorsolateral region of the neural plate or neural tube during neurulation. Their delamination and migration are crucial for embryo development as NCC differentiation is influenced by their final resting locations.

In-Situ Repair Strategies for Defects in Perovskite Solar Cells.

Perovskite solar cells have achieved significant progress in recent years. However, they still have challenges in photovoltaic conversion efficiency and long-term stability. Widespread defects in perovskite films are one of the most important factors leading to their poor performance.

Dual-Grained Lightweight Strategy.

Removing redundant parameters and computations before the model training has attracted a great interest as it can effectively reduce the storage space of the model, speed up the training and inference of the model, and save energy consumption during the running of the model. In addition, the simplification of deep neural network models can enable high-performance network models to be deployed to resource-constrained edge devices, thus promoting the development of the intelligent world. However, current pruning at initialization methods exhibit poor performance at extreme sparsity.

The effect of education level on depressive symptoms in Chinese older adults-parallel mediating effects of economic security level and subjective memory ability.

Background: Depression in older adults needs urgent attention. Increased education level may reduce depressive symptoms in older adults, and that economic security level and subjective memory ability may also have an impact on depressive symptoms in older adults, but the mechanisms between education level and depressive symptoms in older adults are unclear. This study endeavors to investigate the parallel mediating roles of economic security level and subjective memory ability between education level and depressive symptoms in older adults.

Identification and characterization of intermediate states in mammalian neural crest cell epithelial to mesenchymal transition and delamination.

Epithelial to mesenchymal transition (EMT) is a cellular process that converts epithelial cells to mesenchymal cells with migratory potential in developmental and pathological processes. Although originally considered a binary event, EMT in cancer progression involves intermediate states between a fully epithelial and a fully mesenchymal phenotype, which are characterized by distinct combinations of epithelial and mesenchymal markers. This phenomenon has been termed epithelial to mesenchymal plasticity (EMP), however, the intermediate states remain poorly described and it's unclear whether they exist during developmental EMT.

Connection of social anxiety to impaired pattern of cognitive control and underlying motivational deficiencies: Evidence from event-related potentials.

Numerous studies have established a correlation between social anxiety and poor cognitive control. However, little is known about the cognitive control pattern of individuals with high social anxiety (HSAs) and the underlying mechanisms. Based on the Dual Mechanisms of Control framework and the Expected Value of Control theory, this study explored whether HSAs have an impaired cognitive control pattern (Experiment 1) and whether motivational deficiencies underlie the impaired control pattern (Experiment 2).

Association between Oral Health and Depressive Symptoms in Chinese Older Adults: The Mediating Role of Dietary Diversity.

Diet is a modifiable factor in healthy population aging. Additionally, oral health and diet are important factors affecting depressive symptoms. To assess the mediating role of dietary diversity (DD) in oral health and depressive symptoms in older adults, we selected 8442 participants aged ≥ 65 years from the 2018 Chinese Longitudinal Health Longevity Survey (CLHLS) for a cross-sectional study.

Evaluating the link between DIO3-FA27 promoter methylation, biochemical indices, and heart failure progression.

Background: Heart failure (HF) is a disease that poses a serious threat to individual health, and DNA methylation is an important mechanism in epigenetics, and its role in the occurrence and development of the disease has attracted more and more attention. The aim of this study was to evaluate the link between iodothyronine deiodinase 3 promoter region fragment FA27 (DIO3-FA27) methylation levels, biochemical indices, and HF.

Results: The methylation levels of DIO3-FA27_CpG_11.

Cell extrusion - a novel mechanism driving neural crest cell delamination.

Neural crest cells (NCC) comprise a heterogeneous population of cells with variable potency, that contribute to nearly every tissue and organ system throughout the body. Considered unique to vertebrates, NCC are transiently generated within the dorsolateral region of the neural plate or neural tube, during neurulation. Their delamination and migration are crucial events in embryo development as the differentiation of NCC is heavily influenced by their final resting locations.

Amine-releasable Mediator In situ Repair Perovskites for Efficient and Stable Perovskite Solar Cells.

Residual lead iodide (PbI) is deemed to a double-edged sword in perovskite film as small amounts of PbI are beneficial to the photovoltaic performance, but excessive will cause degradation of photovoltaic performance and stability. Herein, an in situ repair strategy has been developed by introducing amine-releasable mediator (methylammonium pyridine-2-carboxylic, MAPyA) to eliminate over-residual PbI and regulate the crystal quality of perovskite film. Notably, MAPyA can be partially decomposed into methylamine (MA) gas and pyridine-2-carboxylic (PyA) during high temperature annealing.

Core and variable antimicrobial resistance genes in the gut microbiomes of Chinese and European pigs.

Monitoring the prevalence of antimicrobial resistance genes (ARGs) is vital for addressing the global crisis of antibiotic-resistant bacterial infections. Despite its importance, the characterization of ARGs and microbiome structures, as well as the identification of indicators for routine ARG monitoring in pig farms, are still lacking, particularly concerning variations in antimicrobial exposure in different countries or regions. Here, metagenomics and random forest machine learning were used to elucidate the ARG profiles, microbiome structures, and ARG contamination indicators in pig manure under different antimicrobial pressures between China and Europe.

Mid-arm muscle circumference and triceps skinfold thickness associated with cardiometabolic disease in Chinese residents: A prospective cohort study.

Background And Aims: The association of cardiometabolic disease (CMD) with body muscle and fat mass remains unclear. Mid-arm muscle circumference (MAMC) and triceps skinfold (TSF) thickness are easily obtained measuring methods for these two body compositions. This study aimed to investigate the association of CMD with MAMC and TSF thickness among Chinese residents.

Identification and characterization of intermediate states in mammalian neural crest cell epithelial to mesenchymal transition and delamination.

Epithelial to mesenchymal transition (EMT) is a cellular process that converts epithelial cells to mesenchymal cells with migratory potential in both developmental and pathological processes. Although originally considered a binary event, EMT in cancer progression involves intermediate states between a fully epithelial and a fully mesenchymal phenotype, which are characterized by distinct combinations of epithelial and mesenchymal markers. This phenomenon has been termed epithelial to mesenchymal plasticity (EMP), however, the intermediate states remain poorly described and it's unclear whether they exist during developmental EMT.

What is the impact of integrated care on the job satisfaction of primary healthcare providers: a systematic review.

Background And Objectives: The integration of care influenced the job satisfaction of healthcare professionals, especially affecting primary healthcare providers (PCPs). This study aimed to perform a systematic review to explore the impact of integrated care on the job satisfaction of PCPs on the basis of Herzberg's two-factor theory.

Methods: This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.

Applied phosphorus is maintained in labile and moderately occluded fractions in a typical meadow steppe with the addition of multiple nutrients.

Phosphorus (P) is a limiting nutrient second only to nitrogen (N) in the drylands of the world. Most previous studies have focused on N transformation processes in grassland ecosystems, particularly under artificial fertilization with N and atmospheric N deposition. However, P cycling processes under natural conditions and when P is applied as an inorganic P fertilizer have been understudied.

Trait anxiety is related to an impaired attention model for controllable threat cues: Evidence from ERPs.

Attentional bias to threat cues is maladaptive for individuals with high trait anxiety (HTA), but may become adaptive when the dangers signaled by these cues can be controlled by timely actions. However, it remains unclear how HTA individuals allocate attention to controllable threat cues. The current study examined whether trait anxiety is associated with an impaired attention model for controllable threat cues and explored the related underlying neural mechanisms.

[Temporal and Spatial Variation Characteristics of Methane, Carbon Dioxide, and Nitrous Oxide Concentrations and the Influencing Factors in Small Aquaculture Pond].

As an important source of greenhouse gases, the changes in greenhouse gas concentrations of aquaculture ponds are not only the basis for accurate quantification of greenhouse gases emissions but are also important for identifying their influencing factors. The spatial and temporal variation characteristics of CH, CO, and NO concentrations and the influencing factors in a typical small aquaculture pond in the Yangtze River Delta were analyzed based on the headspace equilibrium-gas chromatograph method. Except in spring, the concentrations of CH, and NO appeared high at noon or afternoon and were influenced by water temperature.