sp. nov. NIT-TF6 Isolated from Trichloroethene-Dechlorinating Culture with Formate.

A strictly anaerobic bacterium denoted as strain NIT-TF6 of the genus was isolated from a trichloroethene-dechlorinating culture with formate. Cells were straight rods of 1.6-6 µm long and 0.

Recent Advances in Cellulose-Derived Organic Afterglow Materials.

Cellulose-based organic afterglow materials have gained considerable interest due to their low toxicity, cost-effectiveness, and environmental benefits compared to traditional phosphors, with promising applications in anti-counterfeiting, bioimaging, and sensing. Although extensive research has been conducted on cellulose and organic afterglow materials, a comprehensive review systematically discussing the underlying mechanisms and applications of cellulose-based organic afterglow systems remains scarce. This review provides an in-depth analysis of the origin of long-lived afterglow emission in cellulose-based materials, highlighting the critical roles of intramolecular and intermolecular interactions, and proposes strategic design principles for optimizing performance.

Wave succession in the pandemic clone of Vibrio parahaemolyticus driven by gene loss.

While spontaneous mutation and gene acquisition are well-established drivers of pathogen adaptation, the role of gene loss remains underexplored. Here we investigated the emergence and diversification of the pandemic clone of Vibrio parahaemolyticus through large-scale phylogenomic analysis of 8,684 global isolates. The pandemic clone rapidly acquired multiple marker genes and genomic islands, subsequently diverging into successive sublineages mediating independent waves of cross-country transmission, as also observed in Vibrio cholerae.

Assessing causal associations of FGF23 and α-Klotho with urolithiasis using Mendelian randomization analysis.

Fibroblast growth factor 23 (FGF23) and α-Klotho may involve in the occurrence and development of urolithiasis. However, the underlying relationship is still unclear. Mendelian randomization (MR) analysis was performed on datasets from genome-wide association studies (GWASs) to investigate the causal relationship between FGF23, α-Klotho, and urolithiasis.

Porous filter balls alternating internal structure of aerobic composting to drive rapid stabilization and efficient metabolic collaboration.

Co-composting of food waste and agricultural residues offers a promising biotechnological solution for organic solid waste management, where oxygen availability critically governs efficiency. This study evaluated the impact of polypropylene-based porous filter balls (PFB) on composting performance. The results demonstrated that PFB addition elevated peak temperature by 10.

Deciphering Virulence Factors of Hyper-Virulent Associated with Meningitis.

Objective: ( ) is a prevalent pathogenic bacterium involved in meningitis; however, the virulence factors contributing to this disease remain poorly understood.

Methods: The virulence of the A584, isolated from meningitis samples, was evaluated by constructing in vitro blood-brain barrier and in vivo systemic infection models. qPCR, whole-genome sequencing, and drug efflux assays of A584 were performed to analyze the virulence factors.

Kun-Qi birth cohort (KQBC) study for the incidence, aetiology and risk factors of chronic kidney disease in Chinese children: a cohort profile.

Purpose: The Kun-Qi birth cohort (KQBC) was established to investigate the incidence, aetiology and risk factors of chronic kidney disease (CKD) in early childhood (0-6 years). Additionally, the study analysed developmental phenotypic trajectories and their associated paediatric diseases.

Participants: Infants registered or residing in Kunshan and Qidong, Jiangsu Province, who participated in the Chinese Child Healthcare Programme (0-6 years) were enrolled.

Rapid High-Resolution Analysis of Polysaccharide-Lignin Interactions in Secondary Plant Cell Walls Using Proton-Detected Solid-State NMR.

The plant secondary cell wall, a complex matrix composed of cellulose, hemicellulose, and lignin, is crucial for the mechanical strength and water-proofing properties of plant tissues, and serves as a primary source of biomass for biorenewable energy and biomaterials. Structural analysis of these polymers and their interactions within the secondary cell wall has been heavily relying on C-based solid-state NMR techniques. In this study, we explore the application of H-detected solid-state NMR techniques for rapid, high-resolution structural characterization of polysaccharides and lignin, demonstrated on the stems of hardwood eucalyptus.

Robust wavefront sensing method for reflective optical surfaces based on reference light modulation and total variation regularization.

Ptychographic coherent diffraction imaging has been proven effective for measuring reflective optical surfaces. However, its high scanning overlap requirements make it inefficient and cumbersome. This study introduces a lens-free technique that uses reference light coding for more efficient and accurate reflective surface topography measurement.

Calcium phosphate-mineralized nanoplatform for enhanced ferroptosis and synergistic anti-PDL1 therapy in triple-negative breast cancer through multi-pathway targeting.

Ferrotherapy has risen as a promising therapeutic approach for triple-negative breast cancer (TNBC); however, its potency is frequently compromised due to tumor cells' ability to evade ferroptosis via various resistance pathways and insufficient immunogenicity. To overcome these limitations, we have engineered a calcium phosphate-mineralized ferroptosis inducer nanoplatform, termed Lf-PEG-CaP@iFSP1-Brequinar-Erastin-Fe-TA (LP-CaP@iBEFT), designed to augment ferroptosis by simultaneously targeting three key pathways: glutathione peroxidase 4 (GPX4), ferroptosis suppressor protein 1 (FSP1), and dihydroorotate dehydrogenase (DHODH). Once internalized and reached the acidic tumor microenvironment (TME), the nanoplatform discharges its therapeutic payloads, comprising inhibitors of FSP1 (iFSP), brequinar, erastin, and iron ions.

Exosomal tsRNA-Gly-5-0007 may be used as a diagnostic marker for colorectal cancer.

Colorectal cancer is one of the common malignant tumors nowadays, with the highest mortality rate among cancers, and most patients have metastasis when diagnosed. Exosomes contain bioactive molecules such as RNA, which can participate in signal transduction between cells. tRF is a new type of non-coding small RNA, which plays an important role in the progression of cancer.

Association between vaginal microecological alterations and high-risk human papillomavirus infection: a cross-sectional study.

Objective: This study aimed to investigate the correlation between alterations in the vaginal microecological environment and high-risk human papillomavirus (HR-HPV) infection, thereby providing a theoretical basis for clinical prevention and early intervention.

Methods: A total of 854 patients who visited the gynecology outpatient clinic at Wusong Central Hospital, Baoshan District, Shanghai, between June and December 2023 were included. Vaginal secretions were collected for HPV genotyping and microecological analysis.

The autophagy pathway participates in resistance to Candidatus Liberibacter asiaticus infection in Diaphorina citri.

Background: Autophagy is a conserved mechanism by which eukaryotic organisms defend against pathogen infection. However, the molecular mechanisms underlying the role of autophagy in the interactions of insect vectors with the phloem-limited bacterial pathogen remain unclear. The citrus Huanglongbing (HLB)-associated pathogen 'Candidatus Liberibacter asiaticus' (CLas) seriously endangers development of the citrus industry.

Long Noncoding RNA TOB1-AS1 Represses Cervical Cancer Cell Proliferation, Invasion, and Migration via the MicroRNA-27a-3p/Thioredoxin-Interacting Protein Molecular Axis.

Cervical cancer (CC) remains a major global health concern, particularly due to its aggressive nature and limited treatment options in advanced stages. Long noncoding RNA (lncRNA) TOB1-AS1 has been proposed as a tumor suppressor, yet its regulatory mechanism in CC remains unclear. This study aimed to elucidate the role of TOB1-AS1 in CC progression through the miR-27a-3p/thioredoxin-interacting protein (TXNIP) molecular axis.

Synthesis of Perfluorooctyl-Functionalized Halloysite Nanotubes for Dispersive Solid-Phase Extraction of Antimalarial Inhibitors in Whole Blood.

Quinine, chloroquine, and hydroxychloroquine are effective antimalarial drugs that have shown antiviral activity against SARS-CoV-2 in initial in vitro studies. However, their excessive use can lead to adverse effects in humans. Therefore, rapid and accurate analysis of these inhibitors in whole blood samples is crucial for public health, although this presents significant challenges.

Microscopic probing into plasmodesmata (PD) and PD-derived intercellular gateways (PdIGs): Beyond morphology.

Plasmodesmata (PD) are nano-channels in plant cell walls that connect adjacent cells, facilitating the symplastic transport of micro- and macromolecules. In certain tissues, specialized transport requirements drive the formation of intercellular channels in diverse morphologies, compositions and functions, including sieve plate pores, plasmodesmo-pore units, lateral sieve area pore, and flexible gateways. These channels, derived from PD, are referred to as PD-derived intercellular gateways (PdIGs) in this review.

ST-CFI: Swin Transformer with convolutional feature interactions for identifying plant diseases.

The increasing global population, coupled with the diminishing availability of arable land, has rendered the challenge of ensuring food security more pronounced. The prompt and precise identification of plant diseases is essential for reducing crop losses and improving agricultural yield. This paper introduces the Swin Transformer with Convolutional Feature Interactions (ST-CFI), a state-of-the-art deep learning framework designed for detecting plant diseases through the analysis of leaf images.

Polymorphic α-Glucans as Structural Scaffolds in Cryptococcus Cell Walls for Chitin, Capsule, and Melanin: Insights From C and H Solid-State NMR.

Cryptococcus species are major fungal pathogens responsible for life-threatening infections in approximately a million individuals globally each year, with alarmingly high mortality rates. These fungi are distinguished by a distinctive cell wall architecture further reinforced by two virulence-associated layers, melanin and capsule, rendering them insensitive to antifungal agents targeting the cell wall, such as echinocandins. The molecular interplay between these three biomolecular layers remains poorly understood.

Comparative study of electrocardiogram-gated computed tomography (CT) and respiratory four-dimensional CT for radiotherapy in breast cancer patients.

Background: Theoretically, electrocardiogram-gated computed tomography (ECG-CT) can more precisely characterize the motion of a beating heart. However, cardiac motion involves both pulsatile cardiac activity and respiratory-induced movement. ECG-CT has high temporal resolution and fast scanning speed, requiring image acquisition to be completed within a single cardiac cycle.

Plasma exosomal tsRNA may be used as a marker for differential diagnosis of benign and malignant pulmonary nodules.

Background: tRNA-derived small RNAs (tsRNAs) have garnered significant attention in the field of cancer research, however, exosomal tsRNAs remain relatively understudied as potential biomarkers in the pulmonary nodules. This study aims to identify exosomal tsRNAs that are differentially expressed between benign and malignant pulmonary nodules, integrate these findings with other clinical parameters, and develop a novel predictive model to estimate the likelihood of malignancy in pulmonary nodules.

Methods: Exosomes were extracted from plasma of patients with benign pulmonary nodules and malignant pulmonary nodules (early-stage lung cancer), then characterized using transmission electron microscopy (TEM), qNano, and western blot.

Multimodal deep learning-based radiomics for meningioma consistency prediction: integrating T1 and T2 MRI in a multi-center study.

Background: Meningioma consistency critically impacts surgical planning, as soft tumors are easier to resect than hard tumors. Current assessments of tumor consistency using MRI are subjective and lack quantitative accuracy. Integrating deep learning and radiomics could enhance the predictive accuracy of meningioma consistency.

Extended switching endurance of phase change memory through nano-confined cell structure.

Phase change memory has been regarded as a promising candidate for storage class memory application. However, the high switching current and limited switching endurance remain a critical challenge. In this work a switching endurance beyond 1.

Association between iron homeostasis and prognosis in patients with chronic obstructive pulmonary disease: a retrospective analysis from MIMIC-IV database.

Introduction: Accumulating evidence indicates that inflammatory responses can alter iron-related biomarkers, such as serum iron, ferritin, transferrin, and total iron-binding capacity (TIBC). However, in the context of chronic obstructive pulmonary disease (COPD), characterized by airway inflammation, the relationship between its prognosis and iron-related biomarkers has not been comprehensively assessed.

Methods: Clinical data of 611 COPD patients from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database were retrospectively analyzed.