Development Strategies for Influenza Vaccines Utilizing Phage RNA Polymerase and Capping Enzyme NP868R.

Influenza remains a highly contagious respiratory disease with profound global health and economic implications. Although traditional vaccines, including inactivated influenza vaccines (IIVs), live attenuated influenza vaccines (LAIVs), and recombinant subunit influenza vaccines (RIVs), are widely available, their efficacy against emerging viral strains is often limited. This limitation underscores the urgent need for novel vaccine strategies.

Roles of endothelial cells during infection.

Endothelial cells (ECs) integrate immune and vascular functions to promote host defense against pathogens. While previously studied as forming passive flow conduits, ECs are now recognized as active contributors to maladaptive inflammation. During acute infection, ECs may promote tissue pathologies, including hypoxia, acidosis, electrolyte disturbances, and capillary barrier breakdown.

Investigating the Components Change of Steamed Corydalis yanhusuo via Mass Spectrometry Imaging, HS-GC-IMS, HPLC, and Network Pharmacology to Unveil Mechanism for Antichronic Atrophic Gastritis.

Corydalis yanhusuo (YHS), a traditional Chinese medicine (TCM), was commonly steamed before clinical usage for chronic atrophic gastritis (CAG). However, the reasonable steaming time had not occurred, and bioactive components of potential mechanisms of YHS for CAG were still unclear. Thus, the main purpose of this study is to investigate the necessity of steaming processing of YHS before using it in clinical via mass spectrometry imaging (MSI).

Research on the resilience of renewable energy in rural areas-Based on the data from Shandong Province.

China's rural energy system faces three challenges: high structural carbonization, low energy utilization efficiency and insufficient supply stability. These systemic contradictions seriously hinder the coordinated promotion of the "dual carbon" strategy and the rural revitalization process. This study takes 16 cities and cities in Shandong Province as the object and combines GIS technology to construct a biomass and solar energy resource evaluation model.

Ethnomedicine, Botany, Phytochemistry, Pharmacology, Pharmacokinetics, and Toxicity of : A Systematic Review.

(RC) is a traditional Chinese herbal medicine (TCM) known as or in China. This plant is a limited resource distributed in , , , and other places of China. The aim of this paper is to provide a systematic review on the traditional uses, botany, phytochemistry, pharmacology, and pharmacokinetics of this plant.

Multi-Omics Analysis of Chronic Heat Stress-Induced Biological Effects, Liver Injury, and Heat Tolerance Mechanisms via Oxidative and Anti-Inflammatory Pathways in Early-Pregnancy Sows.

The prenatal environment critically influences sow and offspring health, with the liver being highly susceptible to heat stress (HS) and vital for antioxidant defense. However, mechanisms underlying HS impacts on early pregnancy and hepatic adaptation remain unclear. This study applied multi-omics to analyze chronic HS responses in early-pregnancy sows.

Hippocampal Proteomics Reveals the Novel Molecular Profiling of Postnatal Lead (Pb) Exposure on Autism-like Behaviors.

Autism spectrum disorder (ASD) is a multifactorial neurodevelopmental disorder, with lead (Pb) exposure increasingly linked to its risk. However, the molecular mechanisms linking Pb to ASD remain poorly understood. This study established a postnatal Pb-exposed mouse model and employed the three-chamber social test and the marble-burying test to assess ASD-like behavioral phenotypes.

Botany, applications, phytochemistry, and biological activities of Allium mongolicum regel: An update review.

Allium mongolicum, also known as Allium mongolicum Regel or Shā Cōng, is a perennial xerophytic herb that grows in high-altitude desert areas. It is commonly used as a vegetable in daily life with nutritional value beneficially, and it can be made into delicious food with various ingredients, so it is increasingly popular among people of different ages. Interestingly, it is also a traditional Chinese medicine (TCM) of Chinese particularly in Inner Mongolia, and records of its use as a medicine can be found in ancient Chinese books in Ming dynasty.

SFPGCL: Specificity-preserving federated population graph contrastive learning for multi-site ASD identification using rs-fMRI data.

Autism spectrum disorder (ASD) is a severe neurodevelopmental disorder that affects people's social communication and daily routine. Most existing imaging studies on ASD use single site resting-state functional magnetic resonance imaging (rs-fMRI) data, which may suffer from limited samples and geographic bias. Improving the generalization ability of the diagnostic models often necessitates a large-scale dataset from multiple imaging sites.

Optimized plasmid extraction through controlled temperature, prolonged alkaline lysis, and gentle mixing.

Alkaline lysis serves as an effective technique for plasmid extraction. However, its widespread adoption is significantly impeded by inconsistencies in key technical parameters across different documents. To address these issues, we conducted a rheological behavior analysis to understand the pattern of cell lysis and performed a series of plasmid extraction experiments to determine the optimal technical parameters.

Black tea addition enhances the aroma release of rose petals by activating related pathways during the scenting process.

This study investigated the aroma-releasing mechanism of rose during the scenting process with black tea. By quantitative descriptive analysis of sensory evaluations, it was found that 20-h scenting optimized the floral fragrance accumulation while minimizing the undesirable green odors. Meanwhile, neryl alcohol, citronellol, rose oxide, geraniol, linalool, phenethyl alcohol, neryl acetate, citronellyl acetate, eugenol, methyleugenol, and phenethyl acetate were identified as the dominant contributors to the aroma profiles of rose black tea and rose petals.

Mechanisms of Inonotus obliquus (Fr.) Pilát polysaccharides in ameliorating lipid-induced skeletal muscle insulin resistance via PI3K/AKT and AMPK/ACC1/CPT1 signaling pathways.

Ethnopharmacological Relevance: Inonotus obliquus (Fr.) Pilát, a traditional medicinal fungi, has been used to treat diabetes in China and Russia since the 16th century. Recent studies show Inonotus obliquus (Fr.

Acetylation of Hsc70 at K512 inhibits goat ovarian granulosa cell senescence by restoring chaperone-mediated autophagy.

Nonhistone acetylation plays a crucial role in key cellular processes associated with ageing, such as autophagy. Heat shock cognate protein 70 (Hsc70), a vital chaperone protein for chaperone-mediated autophagy (CMA), exerts a molecular chaperone function that is regulated by its acetylation status. However, the effects of this acetylation on CMA and granulosa cell senescence remain unclear.

The Effect of Plasma Treatment on the Mechanical Properties of HDPE/Bamboo Fiber Composites.

In order to improve the interface compatibility of HDPE/bamboo fiber composites, O and N plasma were used to treat the HDPE surface, the effects of plasma treatment power, time, and different flow rates on HDPE surface wettability were analyzed, the optimal process of plasma to improve HDPE surface wettability was determined, and a mathematical model between HDPE surface energy and the plasma treatment process was established. The effect of plasma treatment on the mechanical properties of HDPE/bamboo fiber composites was further studied, and the surface morphology and surface chemical structure of the materials before and after plasma treatment were analyzed. The results show that the surface wettability of HDPE can be significantly improved after O and N plasma treatment, which can further enhance the interface compatibility of HDPE/bamboo fiber composites and improve the impact and tensile strength of the composites (impact strength increased by 19.

BrainFTFCN: Synergistic feature fusion of temporal dynamics and network connectivity for brain age prediction.

Using neuroimaging-derived data for age estimation serves as a prominent approach in comprehending the normal pace of brain development and mechanisms underlying cognitive declines due to aging and neurological diseases. Despite the promise of resting-state functional magnetic resonance imaging (rs-fMRI) for brain age prediction, previous deep learning models have prioritized capturing either the temporal dynamics via time courses (TCs) or the inherent network topology revealed by functional network connectivity (FNC). These fragmented models neglect the complementary information available by synergistically integrating both.

Recombinant humanized collagen ameliorates ischemic myopathy through limiting natural IgM-mediated lectin complement activation.

Despite technological advances in endovascular procedures, therapeutics that provide supportive microenvironments to repair ischemic muscles are limited for the treatment of advanced lower extremity peripheral artery disease (PAD). Here, based on the two major extracellular matrix components in skeletal muscle, the efficacies of recombinant humanized collagen type I and III (rhCol I and rhCol III) in regenerating tibialis anterior muscle after hindlimb ischemia were studied. Repeated intramuscular injections of rhCol I or rhCol III preserved myofiber structure and accelerated myofiber regeneration within one-week after injury.

Synergistic effect of P2, O3 phase on biphasic layered oxide with enhanced electrochemical performance for sodium storage.

Oxides with a layered structure are regarded as prospective candidates for use as cathodes in the next generation of sodium ion batteries. These materials, which exhibit a P2 structure and O3 structure, possess distinctive advantages that give rise to disparate electrochemical performance. Herein, a thermodynamically and kinetically stable P2/O3 biphasic layered oxide with a chemical formula of KNaNiMnO is synthesized using a simplistic high temperature solid-state method.

Structural and functional alterations of the hippocampal subfields in T2DM with mild cognitive impairment and insulin resistance: A prospective study.

Background: Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance (IR) and is often accompanied by mild cognitive impairment (MCI). The detrimental effects of T2DM and IR on the hippocampus have been extensively demonstrated. Few studies have examined the effects of IR on structure and function of hippocampal subfields in T2DM-MCI patients.

Resting-State Brain Function Alteration in Colorectal Cancer Patients.

Background And Purpose: To investigate the abnormal pattern of altered functional activity in the brain and the neuroimaging mechanisms underlying the cognitive impairment of patients with colorectal cancer (CRC) via resting-state functional magnetic resonance imaging (rs-fMRI).

Materials And Methods: CRC patients (n = 56) and healthy controls (HCs) (n = 50) were studied. The participants underwent rs-fMRI scans and the Montreal Cognitive Assessment (MoCA).

Synthesis and Characterization of Multiple Stimuli-Responsive Fluorescent Polymer Hydrogels Based on Terpyridine and -Isopropylacrylamide.

A series of stimuli-responsive fluorescent hydrogels were successfully synthesized via micelle radical copolymerization of hydrophilic acrylamide (AM), hydrophobic chromophore terpyridine-based monomer (TPY), and -isopropylacrylamide (NIPAM). These hydrogels presented blue emissions (423-440 nm) under room temperature, which is caused by the π-π* transition of the conjugated structures. Once the ambient temperature was increased to 55 °C, the fluorescence color changed from blue (430 nm) to pink (575 nm) within 10 min, subsequently to yellow (535 nm), and eventually back to pink.