Genome mining and characterization of a heme-dependent enzyme catalyzing intermolecular Nitrogen-Nitrogen bond formation in hydrazinosuccinic acid biosynthesis.

Nitrogen-nitrogen (N-N) bond-forming enzymes are rare but play vital roles in both primary and secondary metabolism. Guided by a nitric oxide synthase (NOS)-based genome mining strategy, we report the discovery and characterization of a new heme-dependent enzyme system that catalyzes intermolecular N-N bond formation. Using both in vivo and in vitro reconstitution approaches, we demonstrated that a protein complex, comprising a heme enzyme and a 2[4Fe-4S] ferredoxin partner, mediates the coupling of the α-amine group of l-aspartate with inorganic nitrogen oxide species, such as nitrite or nitric oxide, to generate hydrazinosuccinic acid, a key biosynthetic precursor in several natural product pathways.

A Foodborne Outbreak Associated with ST59- t441-SCC IVa Methicillin-resistant Producing Enterotoxins A and B - Puyang City, Henan Province, China, September 2024.

What Is Already Known About This Topic?: () represents a clinically significant pathogen and serves as a common causative agent of foodborne intoxication. The strain ST59 constitutes the predominant clone associated with both community-associated methicillin-resistant (CA-MRSA) and hospital-associated MRSA (HA-MRSA) infections. However, staphylococcal food poisoning (SFP) outbreaks attributed to ST59 MRSA have been documented in only a limited number of Chinese cities through retrospective investigations.

[Mechanism of auraptene in improving acute liver injury induced by diquat poisoning in mice].

Objective: To investigate whether auraptene (AUR) exerts a protective effect on acute diquat (DQ)-induced liver injury in mice and explore its underlying mechanisms.

Methods: Forty SPF-grade healthy male C57BL/6 mice were randomly divided into normal control group (Control group), DQ poisoning model group (DQ group), AUR treatment group (DQ+AUR group), and AUR control group (AUR group), with 10 mice in each group. The DQ poisoning model was established via a single intraperitoneal injection of 40 mg/kg DQ aqueous solution (0.

Suppression of miR-148a-3p Can Promote Bone Healing by Enhancing Angiogenesis-Osteogenesis Coupling Through the PI3K/Akt Pathway.

Previous studies have identified miR-148a-3p as a regulator of both angiogenesis and osteogenesis. However, in vitro findings have been inconsistent. This study aimed to elucidate the role and mechanism of miR-148a-3p in bone physiology using miR-148a knockout (KO) mice.

Revolutionizing cervical cancer treatment: single-cell sequencing of tumor EPCs and immune checkpoints to assess drug sensitivity and optimize therapy.

Background: Cervical cancer's tumor microenvironment (TME) was composed of a diverse array of immune cells that significantly influence tumor progression and response to treatment. Recent advancements in multi-omics and single-cell sequencing had provided valuable insights into the cellular heterogeneity and immune landscape of the TME, revealing critical interactions that shape tumor behavior and therapy outcomes.

Method: This study used multi-omics and single-cell sequencing to explore the immune landscape, cellular heterogeneity, and drug sensitivity in cervical cancer, focused on tumor subtypes and their interactions with immune cells, and aimed to understand therapy responses.

Protein Spatial Structure Meets Artificial Intelligence: Revolutionizing Drug Synergy-Antagonism in Precision Medicine.

Targeted drug design and development, as a core area of modern pharmaceutical research, critically depends on the assessment of protein site druggability as a fundamental component. This review systematically examines the latest research progress and application prospects of drug synergy and antagonism prediction methods that integrate protein three-dimensional spatial structure with artificial intelligence (AI) technologies. This review showcases the molecular biological mechanisms of drug synergism vs antagonism mediated by transcription factors, signal pathway regulation, and membrane transport proteins, and subsequently delves into the molecular structural basis of protein-drug interactions, including precise identification methods for drug binding sites, optimization strategies for molecular docking techniques, and the mechanisms and structural characteristics of multi-target drugs.

Enzyme-crosslinked protocatechualdehyde acetal-modified polyvinyl alcohol hydrogel with acid-responsive and emerging photothermal properties for infected skin wound management.

External pathogens and underlying diseases often complicate the wound microenvironment and hinder healing. The combination of plant polyphenols and photothermal therapy offers multiple advantages for skin wound repair and represents a promising alternative to conventional antibiotics. However, most plant polyphenols are susceptible to chemical degradation and inherently lack photothermal responsiveness.

Identification of a key smooth muscle cell subset driving ischemic cardiomyopathy progression through single-cell RNA sequencing.

Cardiomyopathy encompasses a range of diseases that severely affect the complex functions of the heart, involving structural and functional abnormalities, and is associated with high mortality. Recent studies have highlighted the critical role of ferroptosis in regulating oxidative stress and inflammation in cardiomyopathy. In this study, we established that the C6 S100A4+ SMCs subpopulation is critical by performing an integrated single-cell analysis of the known publicly available data GSE145154.

Molecular insights into glioblastoma progression: role of CHCHD2P9 in tumor heterogeneity and prognosis.

Background: Gliomas are highly aggressive, life-threatening tumors with poor prognosis, and remain a leading cause of mortality among brain cancers. Although the role of mitochondrial proteins in cancer has garnered increasing attention, their specific functions in the nervous system, particularly in gliomas, remain poorly understood.

Methods: We integrated single-cell RNA sequencing with cellular assays and flow cytometry to investigate the molecular characteristics and cellular interactions within glioblastoma subpopulations during tumor progression.

Shopping for others: how does inconsistency in online reviews affect purchase intentions differently?

Introduction: The product reviews on e-commerce platforms often exhibit inconsistencies in consumer opinions. This raises the question: when consumers purchase products for others, do they place greater importance on inconsistent online reviews? To address this issue, this study explores the impact of online review consistency, social distance, and product type on consumer purchase intentions, based on the theories of psychological distance and construal level. It focuses on examining the moderating roles of social distance and product type in this process.

Deciphering the tumor immune microenvironment: single-cell and spatial transcriptomic insights into cervical cancer fibroblasts.

Background: Cervical cancer (CC) remains a significant global health challenge despite advancements in screening, HPV vaccination, and therapeutic strategies. Tumor heterogeneity, driven by epigenetic modifications, affects immune evasion, metastasis, and treatment response. Cancer-associated fibroblasts (CAFs) play a crucial role in CC progression and therapy resistance.

Single-cell RNA sequencing reveals the potential role of Postn(+) fibroblasts in promoting the progression of myocardial fibrosis after myocardial infarction.

Myocardial infarction (MI) is a life-threatening coronary artery-related pathology characterized by sudden cardiac death, often leading to cardiac fibrosis and heart failure (HF). Despite advances in emergency care and treatment measures such as percutaneous coronary intervention (PCI), the mortality rate due to HF following MI remains high, making it the leading cause of death in MI patients. While cardiac fibroblasts are known to be closely associated with the adverse outcomes of cardiac fibrosis and HF post-MI, the cellular landscape of fibroblasts after MI and their role in myocardial fibrosis and HF progression has not been fully explored.

Ectopic expression of GDF15 in cancer-associated fibroblasts enhances melanoma immunosuppression via the GFRAL/RET cascade.

Background: A key aspect of tumor biology is the involvement of cancer-associated fibroblasts (CAFs) in shaping the immunosuppressive microenvironment. However, the dynamic and complex key roles of CAFs in the melanoma immune microenvironment have not been elucidated.

Methods: The CAFs landscape in melanoma was characterized using single-cell RNA-seq and spatial transcriptomics.

Single-cell atlas of endothelial cells in atherosclerosis: identifying C1 CXCL12+ ECs as key proliferative drivers for immunological precision therapeutics in atherosclerosis.

Background: Atherosclerosis (AS) is a chronic inflammatory disease characterized by endothelial dysfunction, monocyte infiltration, smooth muscle proliferation, and extracellular matrix accumulation. Endothelial cell (EC) dysfunction plays a pivotal role in the initiation and progression of AS. Despite progress in traditional research methods, the complexity of cellular heterogeneity within the disease remains poorly understood, necessitating a more refined approach for uncovering disease mechanisms.

Decoding multiple myeloma: single-cell insights into tumor heterogeneity, immune dynamics, and disease progression.

Background: Multiple myeloma (MM) is a biologically heterogeneous malignancy of clonal plasma cells, often progressing from MGUS or smoldering MM. It causes anemia, bone lesions, and immune dysfunction due to abnormal plasma cell expansion in the bone marrow. Neuroinflammatory and neurotrophic factors may influence MM progression by affecting immune cells and the bone marrow niche.

N-Acetylcysteine relieving hydrogen peroxide-induced damage in granulosa cells of sheep.

Sheep ovarian granulosa cells (GCs) play a unique role in the ovary. Damage to GCs can affect the normal development of oocytes. The oxidative stress model was constructed by HOto study the biological changes.

Edaravone inhibits neuronal ferroptosis and alleviates acute Central nervous system injury induced by diquat enhancement of METTL14-mediated m6A methylation of Aldh1l1.

The biological effects of edaravone (Eda), a free radical scavenger, include anti-inflammatory, antioxidant, and neuroprotective qualities. Nevertheless, the function and potential mechanisms of Eda in central nervous system injury damage are still unknown. A rat model of acute diquat toxicity was constructed to observe the pathological changes in brain tissues after diquat administration.

Comparative study on the drug effects and adverse reactions of doxycycline and isotretinoin in the treatment of moderate to severe acne.

To compare the drug effects and adverse reactions of doxycycline and isotretinoin in patients with moderate to severe acne. A retrospective analysis was conducted on 98 patients with moderate to severe acne who were treated in the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine from June 2020 to June 2023. They were divided into group A and group B based on actual medication use, with 49 cases in each group.

Enzyme-Mimetic Zwitterionic Microgel Coatings for Antifouling and Enhanced Antithrombosis.

Blood-contacting devices serve as a mainstay in clinical treatment, yet thrombosis remains a major cause of device failure and poses risks to patient health. In this study, we developed a diselenide cross-linker, ,'-bis(methacryloyl)selenocystamine (BMASC), incorporated into poly(sulfobetaine methacrylate) (PSBMA) microgels (defined as BSM) to create an enzyme-mimetic zwitterionic microgel coating (BSMC). The superhydrophilicity of PSBMA provides outstanding antifouling performance, while the diselenide bonds mimic the catalytic action of glutathione peroxidase (GPx) in generating nitric oxide (NO).

Seawater Membrane Distillation Coupled with Alkaline Water Electrolysis for Hydrogen Production: Parameter Influence and Techno-Economic Analysis.

The production of green hydrogen requires renewable electricity and a supply of sustainable water. Due to global water scarcity, using seawater to produce green hydrogen is particularly important in areas where freshwater resources are scarce. This study establishes a system model to simulate and optimize the integrated technology of seawater desalination by membrane distillation and hydrogen production by alkaline water electrolysis.

Molecular Mechanism of N-Acetylcysteine Regulating Proliferation and Hormone Secretion of Granulosa Cells in Sheep.

Granulosa cells (GCs) are pivotal in the development of ovarian follicles, serving not only as supportive cells but also as the primary producers of steroid hormones. The proliferation of these cells and the synthesis of steroid hormones are crucial for follicular development and atresia. In our study, GCs were isolated using follicular fluid aspiration and subsequently identified through immunofluorescence.

Single-cell analysis unveils cell subtypes of acral melanoma cells at the early and late differentiation stages.

Background: Melanoma, a malignant neoplasm originating from melanocytes, is a form of skin cancer with rapidly increasing global incidence, often exacerbated by UV radiation[1]. Particularly, acral melanoma, characterized by its swift metastasis and poor prognosis, underscores the significance of further research into its heterogeneity. Single-cell sequencing has been widely utilized in the study of tumor heterogeneity; however, research related to melanoma remains to be further refined.

[Protective effect of tumor necrosis factor receptor-associated factor 6 inhibitor C25-140 on acute kidney injury induced by diquat poisoning in mice].

Objective: To investigate the protective effect and mechanism of tumor necrosis factor receptor-associated factor 6 (TRAF6) inhibitor C25-140 on acute kidney injury (AKI) induced by acute diquat (DQ) poisoning in mice.

Methods: A total of 80 SPF grade healthy male C57BL/6 mice were randomly divided into the normal control group, DQ model group, C25-140 intervention group, and C25-140 control group, with 20 mice in each group. The DQ poisoning mouse model was established by using one-time intraperitoneal injection of 1 mL of 40 mg/kg DQ solution.

The cellular signaling crosstalk between memory B cells and tumor cells in nasopharyngeal carcinoma cannot be overlooked: Their involvement in tumor progression and treatment strategy is significant.

Nasopharyngeal carcinoma (NPC) refers to a cancerous tumor that develops in the upper and side walls of the nasopharyngeal cavity. Typically, individuals are often diagnosed with the disease when it has already progressed significantly, and those with advanced NPC tend to have an unfavorable outlook in terms of response rate to targeted treatments and overall clinical survival. Various molecular mechanisms, including Myeloid-derived suppressor cells and factors like PD-L1, have been explored to enhance the outcome of NPC.