Construction of High-Performance Wash-Free Aβ Probes Based on Amino Group Optimization and Amphiphilic Molecular Engineering Strategies.

β-Amyloid (Aβ) protein is a misfolded protein that plays a pivotal role in the pathogenesis of Alzheimer's disease (AD) and is recognized as one of the primary pathological hallmarks of this disorder. Despite significant advancements in developing fluorescent probes for Aβ detection, research on the specific impact of end-group substituents on probe performance and the design of high-performance, wash-free Aβ probes remains limited. In this study, we designed and synthesized a series of water-soluble Aβ fluorescent probes, X-PYOH and X-PYC6, using a styrene pyridinium cation as the core structure by varying the end-group substituents.

Prevalence and clinical correlates of dissociation symptoms in chinese patients with bipolar disorder: An exploratory cross-sectional study.

Background: Increasing studies indicate that dissociation symptoms (DS) are common in bipolar disorder (BD) patients, but little is known about it in Chinese patients. This study aimed to assess the prevalence and clinical correlates of DS in this population.

Methods: Patients diagnosed with BD were recruited between December 2021 and February 2024.

Optimized combination methods for exploring novel space environment-responsive genes and their roles: insights from space-flown and their implications for astronauts.

Purpose: By expanding the catalog of spaceflight-induced molecular signatures in , we aim to identify key molecular features and potential mechanisms underlying space environment-induced health risks to astronauts using as a model organism.

Methods And Materials: We employed an optimized combination algorithm that integrated two co-expression network analysis methods and four machine learning-based models to identify space environment-responsive genes (SEGs) in space-flown . The expression levels and associated biological processes of human orthologues of identified genes were further analyzed using data from the JAXA CFE and NASA Twins studies.

The Isolation and Construction of an Infectious Clone for a Duck Adenovirus Type 3 Strain.

A duck adenovirus type 3 strain, SD2019, was isolated from sick Muscovy ducks in our laboratory in 2019. To study the biological properties of the virus, an infectious clone of the SD2019 strain was successfully established. The plasmid containing the whole genome of DAdV-3 was digested with I and the linearized DNAs were electortransfected into LMH cells; the cells showed cytopathic effects (CPEs) at 96 h post transfection and the rescued virus (rSD2019) was identified by PCR and indirect immunofluorescence assays (IFAs).

Gate-Tunable Polarization Gradient in 2D Polar Semiconductor for Synaptic Transistor.

Neuromorphic computing, inspired by the brain's energy-efficient parallelism processing, offers a transformative alternative to overcome von Neumann bottlenecks. A critical challenge is to replicate the dynamic plasticity of biological synapses using artificial transistor devices capable of nonvolatile, stimulus-tunable charge transport. Existing synaptic transistors mainly rely on mechanisms such as ion migration, ferroelectric switching, floating gate coupling or charge trapping, yet these approaches are inherently constrained by issues like ionic diffusion, polarization fatigue, and charge leakage─that degrade reliability and memory retention.

Transmission routes of cluster 3 Tembusu virus in ducks and chickens.

Tembusu virus (TMUV) is an emerging mosquito-borne flavivirus, primarily transmitted by spp. mosquitoes. The 2010 outbreak of TMUV in ducks revealed that the virus had acquired direct contact and aerosol transmission routes, enabling its rapid spread in duck farms.

Lipid metabolism reprograming by SREBP1-PCSK9 targeting sensitizes pancreatic cancer to immunochemotherapy.

Background: Pancreatic cancer's aberrant lipid metabolism fuels cell growth, invasion, and metastasis, yet its impact on immune surveillance and immunotherapy is unclear. This study investigated how sterol regulatory element-binding transcription factor 1 (SREBP1)-driven lipid metabolism affects the tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC).

Methods: Clinical significance of SREBP1 was assessed in a PDAC cohort from China and The Cancer Genome Atlas (TCGA) cohorts.

Polyketides with Cardioprotective Bioactivities from Sponge-Associated Fungus MA46-5.

One pair of novel enantiomers, gigantdioxin A (+)- and B (-)-, with a skeleton of benzo[][1,3]dioxin; a new acetophenone gigantone A (); a known 3-chlorogentisyl alcohol (), which is the bioprecursor of ; acetophenone (); and chromone derivative () were obtained from the sponge-associated fungus MA46-5. Their structures were established by extensive and in-depth spectral analysis, such as UV, 1D and 2D NMR, and HRESIMS. The absolute configurations of (±)- were deduced by ORD, chiral separation, and experimental and computational ECD.

Breaking Structural Instability and Orbital Symmetry Mismatch in -Block Metal Monochalcogenides for CO Electroreduction via Noninvasive van der Waals Doping.

-block metal monochalcogenides (MX) adopting black phosphorus (BP)-like structures are promising electrocatalysts due to their abundant exposed metal sites and tunable electronic structures. However, their practical application is limited by structural instability arising from lone-pair electron-induced structural distortions, along with an inherent orbital symmetry mismatch with the frontier orbitals of small molecules (e.g.

Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome.

Duck egg-reducing syndrome virus (DERSV) is a novel Avihepatovirus and is responsible for a gradual decline in the laying rate of ducks, decreasing from a peak of 90% to 50%. The development of a rapid detection method for DERSV is crucial for the identification and control of virus infections. In this study, we developed a quantitative reverse transcription PCR (RT-qPCR) assay for detecting DERSV.

Ability and utility of the Physician Well-Being Index to identify distress among Chinese physicians.

Background: Despite the high prevalence of mental stress among physicians, reliable screening tools are scarce. This study aimed to evaluate the capability of the Physician Well-Being Index (PWBI) in identifying distress and adverse consequences among Chinese physicians.

Methods: This cross-sectional online survey recruited 2803 physicians from Southern Mainland China snowball sampling between October and December 2020.

Isolation and Characterization of H1 Subtype Swine Influenza Viruses Recently Circulating in China.

Pigs serve as a mixing vessel for influenza viruses and can independently promote the emergence of pandemic strains in humans. During our surveillance of pig populations from 2021 to 2023 in China, 11 H1 subtype swine influenza viruses (SIVs) were isolated. All viruses were reassortants, possessing internal genes of identical origins (PB2, PB1, PA, NP, M: pdm09/H1N1 origin, NS: North American triple reassortant origin).

The Generation of a H9N2 Avian Influenza Virus with HA and C3d-P29 Protein Fusions and Vaccine Development Applications.

Background: Maternal-derived antibody (MDA) interferes with immune responses, leading to the failure of H9N2 avian influenza vaccinations in poultry. So far, none of the commercially available H9N2 avian influenza vaccines used in poultry have been able to overcome MDA interference.

Methods: To develop a vaccine that can overcome MDA interference, one or multiple copies of the minimum-binding domain (P29) from the complement protein C3d were inserted in between the signal peptide and the head domain of the hemagglutinin (HA) protein on a H9N2 avian influenza virus (A/Chicken/Shanghai/H514/2017, named H514).

New Benzaldehyde Derivatives From the Soft Coral-Associated Epiphytic Fungus Aspergillus Sp. EGF 15-0-3.

Two new benzaldehyde derivatives, aspergitones A (1) and B (2), along with seven known analogues 3-9, were isolated from the soft coral-associated epiphytic fungus Aspergillus sp. EGF 15-0-3. Their structures were elucidated using 1D and 2D nuclear magnetic resonance (NMR) spectroscopy and high-resolution electrospray ionization mass spectrometry (HRESIMS), whereas the absolute configuration of 1 was determined through the experimental and computational ECD analysis.

Integrated spaceflight transcriptomic analyses and simulated space experiments reveal key molecular features and functional changes driven by space stressors in space-flown C. elegans.

The space environment presents unique stressors, such as microgravity and space radiation, which can induce molecular and physiological changes in living organisms. To identify key reproducible transcriptomic features and explore potential biological roles in space-flown C. elegans, we integrated transcriptomic data from C.

hnRNPH1 Inhibits Influenza Virus Replication by Binding Viral RNA.

During the life cycle of the influenza virus, viral RNPs (vRNPs) are transported to the nucleus for replication. Given that a large number of progeny viral RNA occupies the nucleus, whether there is any host protein located in the nucleus that recognizes the viral RNA and inhibits the viral replication remains largely unknown. In this study, to explore the role of hnRNPH1 in influenza virus infection, we knocked down and over-expressed the hnRNPH1 proteins in 293T cells, then infected the cells with the influenza virus.

Occurrence and associated factors of self-reported medical errors among Chinese physicians and nurses: a cross-sectional survey.

Background: Medical errors (MEs) significantly threaten patient safety globally. This study aimed to explore multidimensional factors associated with self-reported MEs among Chinese physicians and nurses.

Methods: A cross-sectional online survey using snowball sampling collected 7197 valid responses from Chinese physicians and nurses between October 2020 and April 2022.

THGB: predicting ligand-receptor interactions by combining tree boosting and histogram-based gradient boosting.

Ligand-receptor interaction (LRI) prediction has great significance in biological and medical research and facilitates to infer and analyze cell-to-cell communication. However, wet experiments for new LRI discovery are costly and time-consuming. Here, we propose a computational model called THGB to uncover new LRIs.

Dimdazenil for the treatment of insomnia: a systematic review and narrative synthesis.

Background: Insomnia is a common sleep disorder affecting approximately 10-20% of adults worldwide. Despite various available treatment modalities, significant gaps remain in improving sleep maintenance and reducing functional impairments.

Objective: To systematically review and synthesize studies on the efficacy and safety of Dimdazenil for the treatment of insomnia.

Efficacy and safety of lumateperone for bipolar depression and schizophrenia: a systematic review and meta-analysis.

This study aimed to evaluate the efficacy and safety of lumateperone in treating bipolar disorder and schizophrenia. A comprehensive literature search was conducted across multiple databases and websites from inception to July 16, 2024, to identify both published and unpublished randomized controlled trials (RCTs). Meta-analyses were performed using random-effects or fixed-effects models depending on statistical heterogeneity.

Surface-modified composites of metal-organic framework and wood-derived carbon for high-performance supercapacitors.

The renewable nature, high carbon content, and unique hierarchical structure of wood-derived carbon make it an optimal self-supporting electrode for energy storage. However, the limitations in specific surface area and electrical conductivity defects pose challenges to achieving satisfactory charge storage in wood-derived carbon electrodes. Therefore, exploring diverse and effective surface strategies is crucial for enhancing the electrochemical energy storage performance.