Construction of A Novel 5-Dye Fluorescent Multiplex System With 30 Y-STRs for Patrilineal Relationship Prediction.

Nowadays, Y chromosome short tandem repeats (Y-STRs) are widely used in forensic medicine practice, which has great significance for ancestry tracing, male lineage evolution, and male paternal relatives. Rapidly mutating Y-STRs (RM Y-STRs) have been shown to have greater potential to distinguish males from males in the patrilineal line. Therefore, a novel 5-dye fluorescent multiplex system with 30 Y-STRs was developed and optimized to screen out more RM Y-STRs and fasting mutating Y-STRs (FM Y-STRs).

Motor imagery decoding network with multisubject dynamic transfer.

Brain computer interface (BCI) provides a promising and intelligent rehabilitation method for motor function, and it is crucial to acquire the patient's movement intentions accurately through decoding motor imagery EEG (MI-EEG) . Because of the inter-individual heterogeneity, the decoding model should demonstrate dynamic adaptation abilities.Domain adaptation (DA) is effective to enhance model generalization by reducing the inherent distribution difference among subjects.

Ultrasmall Hollow Covalent Triazine Framework Nanosphere/Aramid Nanofiber Composite Separator for High-Energy Lithium Metal Batteries.

Sluggish and uneven mass transport in separators significantly accelerates lithium metal batteries (LMBs) degradation. Here, via scaffold-coating synergistic strategy, composite separator integrating heat-resistant, polar aramid nanofiber (ANF) porous scaffold with ultrasmall hollow covalent triazine framework nanosphere (SCTF) coating is fabricated to boost the cycling performance of LMBs across diverse operating conditions. The highly porous ANF layer serves as robust electrolyte reservoir, while the SCTF layer functions as efficient ion redistributor leveraging its extensive interconnected pathways present within staggered layers of intrinsic nanopores with intra- and interparticle open spaces.

Advance in the use of Artificial Intelligence of Pulmonary nodule: evolution, trends, and future directions.

Background: Timely detection and intervention for pulmonary nodules play a vital role in decreasing lung cancer-related deaths. Nevertheless, the precise differentiation between benign and malignant nodules continues to face a major clinical challenge. With the rapid progress of artificial intelligence (AI), significant improvements have been made in the detection, classification, and clinical decision-making related to pulmonary nodules.

The Burden of Nonmalignant Metabolic Dysfunction-Associated Steatotic Liver Disease in the Western Pacific Region: A Systematic Analysis From 1990 to 2021.

The burden of metabolic dysfunction-associated steatotic liver disease (MASLD) is increasing, yet there is little understanding in the Western Pacific Region (WPR). This study aims to report the latest MASLD burden in the WPR from 1990 to 2021. This study obtained nonmalignant and total MASLD incidence, prevalence, mortality, disability-adjusted life years (DALYs), years of life lost (YLLs), and years of healthy life lost due to disability (YLDs) from the Global Burden of Disease (GBD) database, and calculated age-standardized ratios (ASRs) for each indicator.

Hierarchical Phosphorus-Carbon Microarchitectures for Robust and Secure Lithium-Ion Storage.

Phosphorus-carbon composites show promise as high-performance lithium-ion battery anodes but present inherent challenges related to uneven hybridization, poor electrochemical interfaces, and flammability during repeated alloying/dealloying. Here, we propose a countermeasure of hierarchical phosphor-carbon (h-PC) microarchitectures to overcome the aforementioned obstacles. The micromesopores, highly hybridized composites, and enhanced conductivity facilitate accelerated lithium-ion kinetics and robust properties of h-PC, featuring a high-rate capacity of 1040 mAh g @ 10 A g and persistent stability for 1000 cycles of 5 A g.

Prognostic value of residual lymph node status in patients with pathological T0 rectal cancer after neoadjuvant therapy.

Background: A yield pathological T0 (ypT0) classification usually indicates the pathologically complete response of rectal cancer to neoadjuvant therapy. However, lymph node metastasis may still be present.

Objectives: In this study, we aimed to evaluate the prognostic value of residual lymph node status in patients with pathological T0 rectal cancer after neoadjuvant therapy.

Tislelizumab as adjuvant therapy following endoscopic surgery for resectable recurrent nasopharyngeal carcinoma: a randomized clinical trial.

Background: Endoscopic surgery has become the first-line treatment for surgically resectable recurrent nasopharyngeal carcinoma (rNPC), but it is associated with a high risk of postoperative tumor progression. Currently, there is a lack of effective and well-tolerated adjuvant treatment regimens. Thus, the primary objective was to investigate the efficacy and safety of tislelizumab as adjuvant therapy with endoscopic surgery for the treatment of patients with rNPC.

Ileal FXR Knockdown Ameliorates MASLD Progression in Rats via Modulating Bile Acid Metabolism Mediated by Gut Microbiota.

Background And Aim: Metabolic dysfunction associated steatotic liver disease (MASLD) is the predominant cause of chronic liver disease, with dysregulation of bile acid (BA) metabolism and intestinal microbiota being intricately associated with MASLD progression. In this study, we investigated the role of ileal FXR in MASLD progression and BA metabolism in portal blood.

Methods: Sprague-Dawley rats were fed a typical western diet for 20 weeks, followed by local perfusion of AAV2-shNr1h4 to downregulate Nr1h4 expression in ileum tissue.

[Mechanism of Qitu Erzhi Decoction against chemotherapy-induced myelosuppression based on network pharmacology and experimental validation].

To investigate the mechanism of Qitu Erzhi Decoction(QTEZ) in ameliorating chemotherapy-induced myelosuppression and the focus of its decomposed formulae on the effects of hematopoietic cells of the three lineages, respectively. Ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS) was used to identify the components of QTEZ intestinal absorption liquid and obtain the target sites, which were intersected with chemotherapy-induced myelosuppression targets collected from several databases, including OMIM, and an interaction network was established based on network pharmacology for Gene Ontology(GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis. Hematopoietic stem cells of mice were taken after intraperitoneal injection of 5-fluorouracil for myelosuppression modeling and randomly divided into the model group, Qitu Erzhi group, Astragali Radix-Angelicae Sinensis Radix group, Ligustri Lucidi Fructus-Ecliptae Herba group, Psoraleae Fructus-Cuscutae Semen group, and positive drug group, which were given the corresponding traditional Chinese medicine intestinal absorption liquid and the positive drug granulocyte colony-stimulating factor, respectively.

Modulation of renal fibrosis-related signaling pathways by traditional Chinese medicine: molecular mechanisms and experimental evidence.

Renal fibrosis (RF), characterized by excessive deposition of extracellular matrix leading to tissue damage and scar formation, represents a refractory disease and a pivotal pathological basis for the progression to end-stage renal disease. The pathogenesis of RF is intricate, prominently implicating multiple key signaling pathways, including adenosine monophosphate-activated protein kinase/mammalian target of rapamycin (AMPK/mTOR), phosphoinositide 3-kinase/protein kinase B (PI3K/Akt), transforming growth factor-β1/small mother against decapentaplegic (TGF-β1/Smad), toll-like receptor 4/nuclear factor kappa B (TLR4/NF-κB), wingless integrated/β-catenin (Wnt/β-catenin), hypoxia-inducible factor-1α (HIF-1α), Hedgehog, and mitogen-activated protein kinase (MAPK). The current Western medical practices primarily rely on supportive and replacement therapies, which are often costly and suboptimal in efficacy.

Case Report: Endoscope-assisted single-incision double-channel mini-open hemilaminectomy for the treatment of acute thoracolumbar intervertebral disc disease in 11 dogs.

This study aims to explore the feasibility and efficacy of an endoscopic-assisted mini-open hemilaminectomy technique for spinal cord decompression in thoracolumbar intervertebral disc extrusion. A total of 11 dogs with acute thoracolumbar intervertebral disc disease were included in the study, preoperative magnetic resonance imaging (MRI) and computed tomography (CT) were used for precise localization. The surgery was performed using a lateral approach with a skin incision approximately 2 cm in length for a minimally invasive hemilaminectomy of the thoracolumbar spine.

Sparingly-soluble CaCO promotes plant growth and arsenic accumulation in As-hyperaccumulator Pteris vittata: Oxidative stress and gene expression in As metabolisms.

Being a lithophytic plant and arsenic-hyperaccumulator, Pteris vittata can efficiently utilize sparingly-soluble CaCO, which enhances its arsenic (As) uptake and plant growth with the underlying mechanisms being unclear. Here, after growing P. vittata for 14 days under hydroponics containing 50 μM As and 8.

Effect and comparative analysis of urease inhibitor AHA and low pH method on MICP reinforcement of calcareous sand.

In order to alleviate the problem of grouting blockage caused by the immediate action of bacteria and chemical reagents in the process of MICP reinforcement of sandy soil, this paper delays the generation rate of calcium carbonate by adding urease inhibitor AHA to the cementing solution and lowering the pH of the bacterial solution. The mechanism of action of the two methods was explored, and their effects were compared and analyzed. The results indicate that both AHA method and low pH method can effectively delay the generation rate of calcium carbonate and improve the reinforcement effect of MICP under different mechanisms of action.

A novel coumarin-incorporated lanthanide coordination nanoprobe for ratiometric sensing of tetracycline.

Tetracycline (Tc), a broad-spectrum antibiotic for treating bacterial infections, poses significant risks to human health and the environment. This study presents a novel lanthanide coordination probe, AMP/Eu/CMP, for the ratiometric detection of Tc. The pyridine-appended coumarin derivative, CMP, acting as a stable internal reference, combines with AMP and Eu to form the robust ratiometric probe AMP/Eu/CMP.

Cutting-edge technologies illuminate the neural landscape of cancer: Insights into tumor development.

Neurogenesis constitutes a pivotal facet of malignant tumors, wherein cancer and its therapeutic interventions possess the ability to reconfigure the nervous system, establishing a pathologic feedback loop that exacerbates tumor progression. Recent strides in high-resolution imaging, single-cell analysis, multi-omics technologies, and experimental models have opened unprecedented avenues in cancer neuroscience. This comprehensive review summarizes the latest advancements of these emerging technologies in elucidating the biological mechanisms underlying tumor initiation, invasion, metastasis, and the dynamic heterogeneity of the tumor microenvironment(TME), with a specific focus on neuron-glial-tumor interactions in glioblastoma(GBM) and other neurophilic cancers.

Predictive value of pretreatment peripheral blood N/CD4 and N/CD8 ratios for the efficacy of radiotherapy for esophageal cancer.

Background: This study aimed to explore the predictive value of pretreatment peripheral blood immune cell subsets in analyzing the outcomes of patients who underwent radiation therapy for esophageal cancer at their first visit.

Patients And Methods: This study included 72 patients with esophageal cancer (EC) treated at Jiangsu University Hospital from December 2021 to December 2023. Among them, 48 were males and 24 were females, with a median age of 64 years (range: 52-98 years).

Correlating Solvation Free Energy to Electrolyte Properties for Lithium Metal Batteries.

The electrolyte plays a critical role in lithium metal batteries. In particular, ion solvation profoundly impacts key electrolyte properties and battery performance. In this study, we systematically investigate solvation-property relationships in a series of electrolytes with different solvent-diluent ratios.

Arsenic Uptake and Metabolism in Mycorrhizal As-Hyperaccumulator : Symbiotic P Transporters and As Reductases.

Arbuscular mycorrhiza (AM) often protect host plants from As accumulation under arsenic stress; however, the opposite is true for the As-hyperaccumulator . With non-hyperaccumulator as a comparison, the AM colonization, P and As uptake, and genes associated with As metabolism were investigated in after growing 60-day with inoculation under 0 (As), 10 (As), or 100 μM As (As) treatments. Based on the As-induced increase in AM colonization (up to 21%), AM symbiosis promoted growth by 24% and frond P content by 22% in the AM+As treatment than As treatment.

Transcriptome analysis of multiple tissues and identification of tissue-specific genes in Lueyang black-bone chicken.

Systematically constructing a gene expression atlas of poultry tissues is critically important for advancing poultry research and production. In this study, the gene expression profiles of 9 major tissues of Lueyang black-bone chicken were successfully constructed by transcriptome sequencing technology. Through in-depth analysis of transcriptome data, a total of 10 housekeeping genes (HKGs) and 87 marker genes (MGs) were identified.

Evolutionary research trends of polysaccharides from Polygonatum genus: A comprehensive review of its isolation, structure, health benefits, and applications.

Polygonatum sibiricum, valued both as a medicinal and nutritional plant, has long been recognized for its health benefits. Increasing evidence highlights its polysaccharides (PSPs) as key components. As research into the structural characteristics and biological activity of PSPs continues to grow, there is rising interest in developing functional foods that harness their therapeutic potential.

Transferrin Receptor Promotes Endometrial Cancer Proliferation by Activating the Iron-Dependent PI3K/AKT/mTOR Signaling Pathway.

Aberrant iron metabolism is frequently observed in cancers, including endometrial cancer (EC). However, the role of transferrin receptor (TFRC), a key regulator of iron metabolism, remains unclear in endometrial cancer. We found transferrin receptor expression was significantly upregulated in endometrial cancer tissues compared to adjacent nontumor tissues, and high transferrin receptor levels were associated with poor prognosis.

Targeting amino acid metabolism to inhibit gastric cancer progression and promote anti-tumor immunity: a review.

The incidence of gastric cancer remains high and poses a serious threat to human health. Recent comprehensive investigations into amino acid metabolism and immune system components within the tumor microenvironment have elucidated the functional interactions between tumor cells, immune cells, and amino acid metabolism. This study reviews the characteristics of amino acid metabolism in gastric cancer, with a particular focus on the metabolism of methionine, cysteine, glutamic acid, serine, taurine, and other amino acids.