Analytical evaluation of TriVerity, a rapid diagnostic and prognostic host gene expression test performed on the Myrna instrument using RT-LAMP.

Unlabelled: We evaluated the analytical performance of the TriVerity test system, a benchtop system for rapid measurement and interpretation of host messenger RNAs (mRNAs) measured quantitatively from PAXgene Blood RNA specimens using the TriVerity cartridge on the Myrna instrument. In approximately 30 minutes, 3 scores are generated from 29 host mRNAs for the likelihood of a bacterial infection, a viral infection, and illness severity (7-day need for ICU-level care), each falling into 1 of 5 interpretation bands (very low, low, moderate, high, and very high). Reproducibility, precision, limit of detection, linearity, interference, and sample and cartridge stability were determined per Clinical Laboratory Standards Institute standards.

KSHV-encoded vIRF3 Cooperates with Cellular IRF4 to Drive Super-Enhancer Activity through Complex DNA Elements.

The Kaposi's sarcoma-associated herpesvirus (KSHV) oncoprotein vIRF3 is essential for the survival of primary effusion lymphoma (PEL) cells. vIRF3 cooperates with cellular IRF4 to activate super-enhancers (SEs) driving oncogenes including MYC and IRF4 itself. However, the vIRF3/IRF4-responsive DNA sequences underlying this cooperation are unknown.

Association between serum metal levels and NLRP3 gene polymorphisms with cognitive function in aluminum workers: A combined epidemiological and genetic study.

Background: Metals, genes, and cognitive function are closely related. This study evaluated serum metal levels, NLRP3 gene polymorphisms, and their association with cognitive function in aluminum workers.

Methods: A cross-sectional survey was conducted with 478 aluminum workers.

Hydronidone Alleviates Metabolic Dysfunction-Associated Fatty Liver Disease by Inhibiting CD36 Expression.

Currently, the treatment of Metabolic dysfunction-associated fatty liver disease (MASLD) is limited, and this research aimed to investigate the therapeutic effect and molecular mechanism of Hydronidone (HDD) on MASLD. In our research, High fat and High cholesterol diet (HFHC) as well as Methionine-Choline Deficient diet (MCD) were used to establish the MASLD mouse model. Palmitic Acid (PA)-induced mouse primary hepatocytes (mpHCs) and AML12 cell line were used as cell model.

Wavelength selection methods for NIR calibration in petrochemicals: Status, characteristics, and scenarios analysis.

Wavelength selection is the crucial step in constructing a near-infrared (NIR) quantitative analysis model, which plays a crucial role in addressing the curse of dimensionality. Effective wavelength selection eliminates redundant and irrelevant wavelengths, significantly improving the speed and efficacy of data analysis. Given the complexity and collinearity of the spectral data from petrochemical products, numerous wavelength selection methods have been proposed to address the issue of dimensionality.

How surface charges affect interdroplet freezing.

The freezing of droplets on surfaces is closely relevant with various industrial processes such as aviation, navigation, and transportation. Previous studies mainly focus on physiochemically heterogeneous but electrically homogeneous surfaces, on which the presence of vapor pressure gradient between droplets is the predominant mechanism for interdroplet freezing bridging, propagation, and eventual frosting across the entire surface. An interesting yet unanswered question is whether electrostatic charge on surfaces affects freezing dynamics.

Janus Cellular Design Drives Solar-Powered Spatial Lithium Extraction and Water Co-Generation from Salt-Lake Brines.

Lithium plays a vital role in energy storage technologies, with global demand rapidly increasing. Current adsorption-based direct lithium extraction from salt-lake brines holds promise but suffers from low ion extraction efficiency due to sluggish intercalation kinetics and poor selectivity, necessitating repeated cycles that increase water and energy consumption. Here, a Janus cellular-structured solar-powered platform (JCSP) is developed for energy-efficient lithium extraction and sustainable water harvesting.

Association of time in tight range and 1,5-anhydroglucitol in type 2 diabetes.

Aims: Among the novel metrics derived from continuous glucose monitoring (CGM), time in tight range (TITR) has gained increasing attention. Our study aimed to investigate the relationship between 1,5-anhydroglucitol (1,5-AG) and TITR in patients with type 2 diabetes.

Materials And Methods: This cross-sectional study included 1531 moderately controlled patients with type 2 diabetes on a stable treatment regimen.

Polyphosphate- and Antioxidant Peptide-Based Coacervate Delivers miRNA.

RNA-based therapies are often hampered by low RNA stability and cytoplasmic delivery efficiency. Coacervate droplets formed by liquid-liquid phase separation (LLPS) exhibit great potential in drug loading and transfection efficiency for delivering biomacromolecules into the cytoplasm due to their condensed and fluid nature. Here, we developed a type of coacervate droplet as the delivery vector formed by the LLPS of sodium hexametaphosphate (SHMP) and antioxidant peptide SS-31, followed by loading with microRNA-223 (miRNA-223) as a coacervate artificial cell (Coac@miR).

Association between glycemic control indicators with renal function assessed by Tc-DTPA dynamic renal scintigraphy and estimating equations.

Purpose: Our study aimed to analyze the impact of renal function, assessed by various methods of evaluating glomerular filtration rate (GFR), on glycated hemoglobin A1c (HbA1c), glycated albumin (GA), and 1,5-anhydroglucitol (1,5-AG).

Methods: This cross-sectional study analyzed 5113 type 2 diabetes patients. GFR was evaluated using both Tc-DTPA dynamic renal scintigraphy (mGFR) and various estimating equations, including eGFRcr, eGFRcys, eGFRcr-cys, and eGFRc_MDRD.

Ileal FXR Knockdown Ameliorates MASLD Progression in Rats via Modulating Bile Acid Metabolism Mediated by Gut Microbiota.

Background And Aim: Metabolic dysfunction associated steatotic liver disease (MASLD) is the predominant cause of chronic liver disease, with dysregulation of bile acid (BA) metabolism and intestinal microbiota being intricately associated with MASLD progression. In this study, we investigated the role of ileal FXR in MASLD progression and BA metabolism in portal blood.

Methods: Sprague-Dawley rats were fed a typical western diet for 20 weeks, followed by local perfusion of AAV2-shNr1h4 to downregulate Nr1h4 expression in ileum tissue.

New evidence of unequal contribution to health burden reshaping air pollution control in waste-to-energy plants.

Waste-to-energy (WTE) incineration has been long argued for the emission of hazardous flue gas pollutants (FGPs), while the contribution to health burden of individual components is ambiguous, resulting empirical control measures. We constructed a comprehensive framework to assess the health burden by monetizing premature mortalities, combining nine kinds of regulated FGPs from WTE plants. NO and SO were identified as the main contributors to health risk, accounting for around 88.

Multimodal sequencing of neoadjuvant nivolumab treatment in hepatocellular carcinoma reveals cellular and molecular immune landscape for drug response.

A striking characteristic of liver cancer is its extensive heterogeneity, particularly with regard to its varied response to immunotherapy. In this study, we employed multimodal sequencing approaches to explore the various aspects of neoadjuvant nivolumab treatment in liver cancer patients. We used spatially-resolved transcriptomics, single- and bulk-cell transcriptomics, and TCR clonotype analyses to examine the spatiotemporal dynamics of the effects of nivolumab.

A noncanonical role of SAT1 enables anchorage independence and peritoneal metastasis in ovarian cancer.

Anchorage-independent survival of ovarian tumor cells in ascites is the initial and critical step for peritoneal metastasis. How ovarian tumor cells achieve anchorage independence remains unclear. Here we show that a noncanonical role of spermidine/spermine N1-acetyltransferase 1 (SAT1) dictates anchorage-independent cell survival and potentiates metastatic dissemination in ovarian cancer.

Research progress on the regulatory role of different cell death pathways in metabolic-dysfunction-associated steatotic liver disease.

Metabolic dysfunction associated steatotic liver disease (MASLD) is one of the most common chronic liver diseases that pose a significant threat to human health. An essential process in developing various diseases, including MASLD, is programmed cell death, a regulated and controlled mechanism that eliminates damaged or unnecessary cells. It is a ubiquitous process during organismal development and represents an active, orderly form of cell death.

The Relationship Between Glycated Albumin and Time in Tight Range in Type 2 Diabetes.

Aims: Among the new glucose metrics derived from continuous glucose monitoring, the concept of time in tight range (TITR) has gained increasing attention. We aimed to assess the association between TITR and traditional glycemic indicators, such as glycated albumin (GA).

Methods: A total of 310 patients with type 2 diabetes on a stable glucose-lowering regimen over the previous 3 months were enrolled.

Ultrasound Reference Values of Ventriclo/Hemispheric Ratio and Evans Ratio Based on Most Correlated Covariates for Neonates Born at 25-41 Gestational Weeks: A Prospective Cross-Sectional Study.

Objectives: This study aimed to establish ultrasound reference values for the ventriculo/hemispheric ratio (V/HR) and Evans ratio in neonates between 25 and 41 weeks of gestation, based on the most correlated covariates (MCCs).

Methods: Data for V/HR and Evans ratio were collected from 1-to 7-day-old neurologically healthy neonates born or hospitalized at Hunan Provincial Maternal and Child Health Care Hospital and Yueyang Maternal and Child Health Hospital between March 2023 and September 2024. Max-min normalized quantile regressions were utilized to analyze the correlation between exploratory and dependent variables and to identify the MCCs.

YBX1/CD36 positive feedback loop-mediated lipid accumulation drives metabolic dysfunction-associated steatotic liver disease.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a common chronic liver disorder mainly caused by an imbalance in lipid homeostasis. Y-box binding protein 1 (YBX1) participates in multiple pathophysiological processes, including embryonic development, tissue repair, liver disorders, and energy metabolism. The objective of this study is to investigate the mechanisms underlying MASLD and characterize the role of YBX1 in MASLD.

Elevated prolactin levels before endometrial transformation negatively impact reproductive outcomes in frozen embryo transfer cycles under hormone replacement therapy.

Introduction: Prolactin (PRL) plays a key role in the regulation of reproductive functions. However, its impact on outcomes in infertility women undergoing assisted reproductive technology remains unclear. This study aimed to examine the relationship between PRL levels and reproductive outcomes in frozen embryo transfer (FET) cycles under hormone replacement therapy (HRT).

Study on landslide hazard risk in Wenzhou based on slope units and machine learning approaches.

Landslides are a prevalent and devastating form of geological disaster. These events occur when gravity causes rock and soil masses to slide along specific surfaces or zones, often triggered by intense rainfall, seismic activity, or human engineering activities. Assessing landslide hazard risk is crucial for effective disaster management, yet traditional approaches often rely on administrative or grid units, which lack the granularity needed for site-specific hazard management.

Early sepsis recognition: a pilot study using a rapid high-multiplex host-response mRNA diagnostic test.

Background: Early sepsis diagnosis is essential to allow timely initiation of adequate care. The TriVerity™ Test, performed on the Myrna™ Instrument, is the first rapid high-multiplex host-response mRNA diagnostic test that supports clinical decision-making by evaluating the likelihood of bacterial and/or viral infections and severity of illness. We present findings of the first, proof of concept, real-world evaluation in an emergency department (ED).

ACAT1-Mediated ME2 Acetylation Drives Chemoresistance in Ovarian Cancer by Linking Glutaminolysis to Lactate Production.

Lactate derived from aerobic glycolysis is crucial for DNA damage repair and chemoresistance. Nevertheless, it is frequently noted that cancer cells depend on glutaminolysis to replenish essential metabolites. Whether and how glutaminolysis might enhance lactate production and facilitate DNA repair in cancer cells remains unknown.

Assessment of landslide susceptibility in watersheds during extreme rainfall using a complex network of slope units.

Rainfall-induced landslides present significant challenges in regional landslide prediction and management. Traditional regional landslide susceptibility assessment models often evaluate individual units in isolation, neglecting the hydrological connections between slope units within a watershed. This approach fails to account for the occurrence of landslides in groups.