Failure Mode and Effects Analysis (FMEA) integrating quality indicators for risk assessment of the total testing process in human papillomavirus genotyping testing: a proactive risk analysis model for molecular diagnostics.

Objectives: To develop a proactive risk assessment model for human papillomavirus (HPV) genotyping testing by integrating Failure Mode and Effects Analysis (FMEA) with quality indicators (QIs), ensuring compliance with ISO 15189:2022 and improving diagnostic accuracy.

Methods: A multidisciplinary team designed and performed detailed FMEA across pre-analytical, analytical, and post-analytical phases of HPV genotyping testing. To improve objectivity, we integrated Sigma metrics into the FMEA framework through a molecular diagnostics-specific model of QIs (MQI).

Cooperative Design of Magnesium Ions with Cations or Anions in 2D VS Electrodes.

Two-dimensional vanadium disulfide (VS) is widely used in electrode materials for aqueous batteries but still suffers from limited capacity and rate. We designed an interlayer reaction based on VS in synergy of Mg with conventional cations or anions (H, NH, OH, NO, F, Cl, and SO) (dividing these ions' workings into dual-ion batteries, Mg-anion co-(de)intercalation, and gap-filling). Relatively more suitable paired ions were recommended for each of the three mechanisms.

Bio-inspired ion channels for suppressing interfacial parasitic reactions and enabling low-energy ion desolvation in aqueous supercapacitors.

The sluggish de-solvation reaction kinetics of hydrated ions and the occurrence of undesired water electrolysis on the electrode-electrolyte interface pose significant challenges to the practical deployment of aqueous supercapacitors. Interestingly, biological ion channels exhibit remarkable abilities to facilitate the de-solvation and low-energy transport of hydrated ions, and these are achieved through their size-limited confinement effects and electrostatic interactions. Inspired by such transit mechanisms of ion channels, we propose an interesting strategy to facilitate the rapid desolvation of electrode surface ions with low-energy transport.

[ regulates manganese accumulation in ].

Stresses induced by the deficiency or excess of trace mineral elements, such as manganese (Mn), represent a common limiting factor for the production of crops like . To identify key genes involved in Mn allocation in . and elucidate the underlying mechanisms, a member of the metal tolerance protein (MTP) family obtained in the previous screening of cDNA library of .

Exploration of the Therapeutic Time Window for Thrombectomy in Rat Models of Middle Cerebral Artery Ischemia-Reperfusion.

Objective: To investigate the therapeutic time window of middle cerebral artery occlusion (MCAO) reperfusion in rats and the potential mechanism of injury beyond the time window.

Methods: Male Sprague Dawley (SD) rats were randomly divided into eight groups: a sham operation group, six ischemia-reperfusion groups (reperfusion initiated 1, 2, 3, 4, 5, and 6 h after infarction, respectively), and a 24-h infarction group without reperfusion therapy. Neurological function scores were assessed 24 h after reperfusion, and then brain samples were collected to explore the mechanisms.

mA methylation-mediated lncRNA RNF144A-AS1 promotes hepatocellular carcinoma progression through the miR-1301-3p/RNF38 pathway.

Background: Globally, HCC is still one of the most common cancers. N6-methyladenosine (mA) modifications and long-stranded noncoding RNAs (lncRNAs) play key roles in regulating HCC progression. The role of the lncRNA RNF144A-AS1, a newly identified lncRNA, in HCC is unclear.

Building gene regulatory networks to uncover tolerance mechanisms to salt and drought in Tamarix hispida.

Salt and drought stress significantly impact plant survival. The gene regulatory network (GRN) is crucial for elucidating stress tolerance mechanisms and identifying key transcription factors (TFs) involved in stress regulation. This study developed a three-layered GRN in Tamarix hispida for both salt and drought stress, identifying regulatory relationships among genes using a partial correlation coefficient algorithm.

Which Intraoperative Monitoring Method More Accurately Predicts Voice Change After Thyroid Nodule Ablation: Transcutaneous Laryngeal Ultrasonography Versus Subjective Voice Assessment.

Objective: Although subjective voice assessment (SVA) is recommended for the safety of thermal ablation (TA) for thyroid nodules (TNs), it is not reliable enough to predict voice quality. Transcutaneous laryngeal ultrasonography (TLUSG) can directly detect vocal cord mobility; however, only limited data on TA are available. Our aim was to determine a better method for predicting voice change after TA by comparing SVA and TLUSG.

GPX4: A Key Regulator of Mitochondrial Function and Glycolysis in Pulmonary Artery Smooth Muscle Cells.

Pulmonary arterial hypertension (PAH) is a progressive cardiovascular disease characterized by elevated pulmonary arterial pressure and vascular remodeling. However, the underlying mechanisms remain unclear. This study reveals a novel mechanism by which oxidative stress reduced glutathione peroxidase 4 (GPX4) expression in both rat and human pulmonary arterial smooth muscle cells (PASMCs), establishing a reciprocal regulatory relationship between GPX4 and reactive oxygen species (ROS).

Clinical Phenotype Spectrum in Two Large Chinese Families With Rippling Muscle Disease Caused by CAV-3 c.80G>A.

Rippling muscle disease (RMD) is a rare benign myotonic myopathy caused by pathogenic variants in the caveolin-3 (CAV-3) gene and is characterized by highly irritable muscles, transient localized muscle bulges, and ripple-like contractions. There is still a lack of systematic understanding of the phenotypic spectrum of RMD. In this study, clinical data were collected from patients with RMD.

Human gaze-based dual teacher guidance learning for semi-supervised medical image segmentation.

In the field of medical image segmentation, the scarcity of labeled data poses a major challenge for existing models to accurately perceive target regions. Compared with manual annotation, gaze data is easier and cheaper to obtain. As a classical semi-supervised learning framework, mean-teacher can effectively use a large number of unlabeled medical images for stable training through self-teaching and collaborative optimization.

TyG-BMI as a predictor of ischemic stroke over 10 years in middle-aged and older adults: findings from the China cardiometabolic disease and cancer cohort (4C) study.

Background: Ischemic stroke (IS) is a leading cause of death and disability, imposing a significant economic burden globally. Research has demonstrated that insulin resistance (IR) plays a key role in the development of atherosclerosis, platelet dysfunction, and a hypercoagulable state, all of which contribute to the pathogenesis and progression of IS. The triglyceride-glucose (TyG) index serves as a practical tool for assessing insulin sensitivity, with previous studies exploring its correlation with IS.

Enhancing Long-Term Survival and Self-Renewal of Primary Hepatocytes via Rapid Spheroid Formation Using Rocker System through Co-Culturing with HUVEC Over-Expressing RSPO1.

Background: Porcine primary hepatocytes are vital for liver therapy due to their procurement ease and robust functions. However, they rapidly dedifferentiate in vitro, challenging large-scale maintenance. This study aims to enhance the long-term survival and self-renewal of primary porcine hepatocytes by generating spheroids using a rocker system and optimizing conditions with HUVECs and Roof plate-specific spondin 1 (RSPO1).

CO hydrogenation to HCOOH catalyzed by aqueous Pd needle assembly.

Carbon dioxide (CO) conversion to liquid fuels has attracted great attention due to the current environmental concerns and energy crisis. However, the selective conversion of CO to target liquids is formidably challenging due to the chemical inertness of CO. We theoretically and experimentally confirm that the bending of Pd-Pd bond can breaks the asymmetric potential well and facilitate CO adsorption.

Chelerythrine Chloride Alleviated Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting Glycolytic Pathway Through Targeting Glyceraldehyde-3-Phosphate Dehydrogenase.

Acute lung injury (ALI) is a fatal respiratory disease caused by excessive inflammation. Chelerythrine chloride (CH), an isoquinoline alkaloid, exhibits diverse biological activities. The research focused on assessing CH's therapeutic effects against LPS-mediated ALI in mice and its underlying mechanisms.

Genome-wide identification and functional characterization of phosphatidylethanolamine-binding protein family genes in rubber dandelion (Taraxacum kok-saghyz Rodin).

The domestication breeding of rubber dandelion (Taraxacum kok-saghyz, TKS), a natural rubber (NR)-producing plant that can produce >20 % NR and 40 % inulin in its roots, is of great agricultural and industrial significance. The phosphatidylethanolamine-binding protein (PEBP) family members play important roles in floral induction and sugar metabolism. Therefore, they can be used for the domestication breeding of TKS to enhance NR and inulin production.

The CsphyB-CsPIF4-CsBRC1 module regulates ABA biosynthesis and axillary bud outgrowth in cucumber.

Shoot branching is an important crop agronomic trait that directly affects plant architecture and crop productivity. Although phytochrome B (phyB), BRANCHED1 (BRC1), and abscisic acid (ABA) mediate axillary bud outgrowth, it is unknown if there is any integrating factor among them in the Plantae. We report that mutation of CsphyB or inactivation of CsphyB by shade inhibits lateral bud outgrowth in cucumber.

The GSK3β inhibitor attenuate APAP-induced liver injury by regulating PGC1α-mediated oxidative stress.

Acetaminophen (N-acetyl-p-aminophenol, APAP), is the most frequent cause of drug-induced liver injury. Reducing APAP-induced oxidative stress is an effective strategy to improve acute liver injury. Glycogen synthase kinase-3β (GSK3β), is sensitive to redox homeostasis and is involved in oxidative stress-related injuries.

Exogenous [Pyr]apelin-13 prevents bupivacaine-induced cardiotoxicity via the apelin (APJ) receptor.

Background: Abnormal energy metabolism is an important mechanism in the development of bupivacaine-induced cardiotoxicity. Apelin, a peptide derived from adipocytes, plays a pivotal role in both energy metabolism and the regulation of the cardiovascular system, thereby potentially linking it to bupivacaine-induced cardiotoxicity.

Methods: Our study employed both an Sprague-Dawley neonatal rat cardiomyocyte-based bupivacaine toxicity model and an bupivacaine-induced adult male Sprague-Dawley rat asystole model.

Inhibition of HPSE/SDC-2 axis-induced epithelial-mesenchymal transition for treating IC/BPS.

Interstitial cystitis/bladder pain syndrome (IC/BPS) plagues patients and clinicians with its unclear etiology and pathogenesis, and ineffective treatments. Destruction of epithelial tissue and proliferation of interstitial tissue are typical pathological features of IC/BPS, in which epithelial-mesenchymal transition (EMT) may play an important role. Both the increased urination frequency observed in mice with acute cystitis induced by cyclophosphamide (CYP) and the disruption of the anti-leakage barrier in urothelial cells induced by LPS are associated with the occurrence of EMT.

Ultrastretchable, Antifreeze, Self-Healing, Conductive Hydrogel-Based Triboelectric Nanogenerators for Human Motion Detection and Signal Transmission.

Wearable electronic devices based on conductive hydrogels have gained attention for applications in health monitoring, electronic skin, and human-computer interaction. However, limited functionality hinders the development of conventional hydrogels. Herein, a multifunctional poly(acrylic acid)/carboxymethyl cellulose/polydopamine-ethylene glycol (PAA/CMC/PDA-EG) hydrogel is developed via free radical polymerization initiated by a PDA-Fe redox system and dynamic metal coordination.

Bacterial biofilm on peritoneal dialysis catheters: A retrospective observational study.

Biofilm formation significantly impacts infection risks in peritoneal dialysis (PD) patients. However, few studies focused on biofilms on PD catheters. This study aims to explore the formation and characteristics of bacterial biofilm on PD catheters.

Impact of online negative and positive reviews on app innovation.

Past studies have suggested that online reviews positively impact app innovation. However, extant research has not yet explored the distinct impacts of online negative and positive reviews on app innovation. Based on signaling theory and negative bias, this study empirically examines the effects of online negative reviews versus online positive reviews on app innovation by using panel data from the iOS App Store in China.

Arabidopsis Golgi Anti-Apoptotic Proteins Confer Drought Tolerance to Water Deficiency by Enhancing Degradation of the Aquaporin PIP2;7.

Water retention is one of the important factors for plants to survive under various stress conditions. In plants, the so-called plasma membrane intrinsic proteins (PIPs) are the main water channels that regulate the water status of plants. Membrane trafficking contributes to the functional regulation of major PIPs and is crucial for abiotic stress resilience.