A real-world safety evaluation of new oral anticoagulants in elderly patients: evidence from the Adverse Drug Reaction Monitoring Center of Henan Province.

Background: The use of new oral anticoagulants (NOACs) is becoming increasingly widespread, but data on their adverse reactions are still incomplete. Further analysis based on data from the Drug Adverse Reaction Center is needed to guide safe clinical use.

Methods: A retrospective analysis was performed on 281 cases of rivaroxaban and 48 cases of dabigatran etexilate-related ADR reported by medical institutions collected by a provincial Food and Drug Administration from 2018 to 2023.

The atlantoaxial distance ratio (AADR): proposal and validation of a novel radiographic parameter for the evaluation vertical atlantoaxial dislocation in a preliminary descriptive study.

Objectives: Vertical atlantoaxial dislocation (VAAD) is a clinically significant but often overlooked component of atlantoaxial instability, yet a reliable, universally applicable radiographic parameter for its quantification is lacking. This preliminary study proposes and validates a novel metric, the atlantoaxial distance ratio (AADR), for the radiographic assessment of VAAD.

Materials And Methods: The AADR is calculated on lateral cervical radiographs as the ratio of the mean vertical distance from the C1 superior tubercles to the C2 inferior endplate, divided by the anteroposterior diameter of the C2 inferior endplate.

Cinobufagin overcomes bortezomib resistance in multiple myeloma strains by targeting SEC62/TRPM4-mediated NECSO.

Background: Necrosis by sodium overload (NECSO) is a poorly understood, novel form of cell death implicated in cancer. TRPM4 is the only protein currently linked to NECSO and is downregulated in bortezomib-resistant multiple myeloma (MM) patients and cell lines.

Purpose: To investigate the potential of cinobufagin to overcome bortezomib resistance in MM and elucidate its underlying mechanism of action, particularly regarding TRPM4 and NECSO induction.

Energy Deficiency-Induced ATG4B Nuclear Translocation Inhibits PRMT1-Mediated DNA Repair and Promotes Leukemia Progression.

Metabolic alterations and genomic instability are the hallmark features of many cancers. However, the precise mechanisms underlying the intricate links among these processes remain largely unknown. Here, a molecular mechanism is presented that regulates the interplay between cellular energy metabolism and DNA repair.

A nomogram model for predicting maternal cardiovascular complications and neonatal adverse outcomes in pregnant patients with pulmonary arterial hypertension.

Background: Pulmonary arterial hypertension (PAH) during pregnancy significantly increases maternal and fetal mortality risk. We developed nomogram prediction models from retrospective data to assess maternal cardiovascular risks and neonatal adverse outcomes.

Methods: Our study included 170 pregnant women, divided into training (70%) and validation (30%) sets.

Membranous nephropathy in ankylosing spondylitis: a rare case report.

Ankylosing spondylitis (AS) is a chronic inflammatory disease that typically affects the axial skeleton. Renal involvement is rare in AS, only occurring in 5-13% of AS patients. Membranous nephropathy (MN) in patients with AS is extremely rare.

Gestational Exposure to Particulate Matter and Preeclampsia Risk: Evidence from a Meta-Analysis and Mendelian Randomization Study.

Preeclampsia (PE) is a severe hypertensive disorder that occurs during pregnancy. Exposure to particulate matter (PM), particularly fine particles such as PM2.5/PM10, has been investigated as a potential environmental risk factor for its development.

Identification of a Fetal De Novo Splice Variant in Associated With Growth and Skeletal Abnormalities.

Objective: To report a fetus with -related syndrome caused by a novel heterozygous variant, highlighting the importance of early genetic diagnosis in prenatal care.

Methods: The clinical and genetic data of a fetus with a complex combination of clinical signs and a novel heterozygous variant were collected and have been summarized in this study. The potential pathogenic variant was identified throughout the whole exome sequencing and the effects of candidate variants were further validated by a minigene splicing assay.

Intelligent Gene Delivery System Functionalized Metal Implants for Fracture Repair via Remodeling Mitochondrial Homeostasis.

Delayed union and nonunion of fractures are primarily attributed to the impaired osteogenic activities of bone marrow mesenchymal stem cells (BMSCs). Gene therapy targeting BMSCs is emerging as a promising strategy to promote fracture healing. However, the oxidative stress and mitochondrial dysfunction in BMSCs considerably weaken the efficacy of gene therapy.

THBS4 downregulation alters trophoblast function in preeclampsia via the TGF-β1/Smad signaling cascade.

Preeclampsia (PE) is a severe complication specific to pregnancy, characterized by the onset of hypertension after 20 wk of gestation, often accompanied by proteinuria and damage to important maternal organs. The abnormal invasive function of trophoblast cells leads to impaired remodeling of uterine spiral arteries, shallow placental implantation, and inadequate uteroplacental perfusion, which is one of the important factors in the development of PE. This study aimed to explore key molecules that affect the invasive and migratory functions of trophoblast cells.

An ultrathin polymer-based solid-state electrolyte membrane with high conductivity for lithium metal batteries operable at -25 °C.

Polymer-based solid-state electrolytes offer the advantages of achieving ultrathin thickness through straightforward processing, which is beneficial for improving the energy density of lithium metal batteries. However, significant challenges remain in accelerating Li transport within polymers at room and low temperatures, as well as in improving the electrolyte/electrode interfacial stability. In this work, a novel approach that combines multifunctional inorganic fillers and plasticizers in solid-state polymer electrolytes (SPEs), resulting in ultrathin solid electrolytes with a thickness of 13 μm and exceptional Li transport performance at room temperature and low temperature.

SUMOylation is a Translatable Target in Hypoxic MNPs Regulating Retinal Vasculopathy.

Retinal vasculopathies pose a devastating threat to human health. While anti-VEGF therapy situates the first-line treatment for patients, the clinical efficacy is limited by suboptimal response and potential risks raised by long-term high-dosage use. Neurovascular unit uncoupling is recognized as a key mechanism contributing to pathological neovascularization, yet how immune components get involved is less appreciated.

CD97-directed CAR-T cells with enhanced persistence eradicate acute myeloid leukemia in diverse xenograft models.

Chimeric antigen receptor (CAR)-T therapy on acute myeloid leukemia (AML) is hindered by the absence of a suitable tumor-specific antigen. Here, we propose CD97 as a potential target for CAR-T therapy against AML based on its broader and higher expression on AML cells compared to normal hematopoietic stem and progenitor cells (HSPCs). To resolve the fratricide problem caused by CD97 expression on T cells, we knock out CD97 in CAR-T cells using CRISPR-Cas9.

Injectable thermosensitive hydrogel system based on hyaluronic acid and methylcellulose for the synergistic therapy of traumatic spinal cord injury.

Spinal cord injury (SCI) is a neurological disease with a high rate of disability. Inflammation plays a key role in all stages of pathological of SCI and interacts with ferroptosis to induce deterioration. Methylprednisolone sodium succinate (MPSS) is currently the only drug in clinical to treat SCI through anti-inflammation, but due to the lack of efficacy and systemic adverse reactions, the drug therapy of SCI is still limited.

Endoscopic thyroidectomy for central lymph node dissection-is there a difference in the number of lymph node dissections performed through different surgical approaches? A retrospective cohort study and pooled data research.

Background: Endoscopic thyroidectomies are commonly performed for thyroid cancer. Previous studies indicated that trans-areola approach is inferior in central lymph node dissection (CLND) due to clavicle protruding. The present study aimed to compare different surgical approaches of endoscopic thyroidectomies regarding surgical outcomes.

Venetoclax plus azacitidine with or without homoharringtonine followed by allogeneic haematopoietic cell transplantation in patients with relapsed/refractory acute myeloid leukaemia: A multicentre cohort study.

The appropriate salvage regimen followed by allogeneic haematopoietic stem cell transplantation (allo-HSCT) for relapsed/refractory (R/R) acute myeloid leukaemia (AML) patients remains unclear. Three hundred and fifty R/R AML patients receiving venetoclax and azacitidine (VA) regimen or VA plus homoharringtonine (VAH) regimen as salvage therapy were enrolled in this study, with a higher composite complete remission rate in the VAH group (69.9%) than in the VA group (46.

Tissue-resident microbiota signature in nasopharyngeal carcinoma.

Background: Emerging evidence reveals that microbiota plays a crucial role in multiple cancers. Nasopharyngeal carcinoma (NPC) tissues harbour microbiota, highlighting the need to investigate the clinical implications of tissue-resident microbiota in the development of NPC. Here, we aim to clarify the specific profile of tissue-resident microbiota and its influence on NPC outcomes.

Microfluidics-Based High-Throughput Single-Cell Analysis of Reactive Oxygen Species and T Cell Exhaustion.

Reactive oxygen species (ROS) play a significant role in cellular signaling and oxidative stress, with elevated levels often linked to T cell exhaustion in various pathological conditions, including cancer. However, the relationship between ROS and T cell exhaustion in acute myeloid leukemia (AML) remains unexplored. To address this, we developed a high-throughput single-cell platform─T cell exhaustion and reactive oxygen species analyzer (TEROSA).

Investigation of the mechanism of ambivalent professional identity formation: A directed content analysis of reflective assignments.

Background: Ambivalent professional identity (API)-the coexistence of identification and dis-identification with one's profession-undermines motivation and satisfaction. Research on API among medical interns remains limited. This study applies social cognitive theory (SCT) to explore the formation mechanism of API in Chinese medical interns and proposes strategies to enhance professional identity.

Investigating the status and influencing factors of professional identity formation among Chinese medical interns: a mixed methods study.

Background: Professional Identity Formation (PIF) involves a movement through a series of conceptually distinct developmental stages. Kegan's theory of PIF is divided into 6 phases (stages 0-5). It is believed that students undergo stages 2-4 (imperial, interpersonal, institutional) during medical education.

Singapore grouper iridovirus VP018 abrogates the interferon response by targeting STING-TBK1-IRF3 axis.

Singapore grouper iridovirus (SGIV), causes high mortality rate in grouper aquaculture. Previous study showed that SGIV VP018 was a highly abundant virulence factor, but the potential mechanism underlying the actions of VP018 still remained largely uncertain. Here, we firstly demonstrated that VP018 was interacted with major capsid protein (MCP) and VP075, and recruited into viral assembly sites in SGIV infected cells.

Gas Vesicle-Assisted Ultrasound Imaging for Effective Anti-Tumour CAR-T Cell Immunotherapy Efficacy in Mice Model.

Introduction: Real-time tracking of Chimeric Antigen Receptor (CAR) T cell trafficking provides crucial information regarding the targeted migration, infiltration, and persistence of CAR-T cells within the tumour microenvironment (TME). This information is invaluable for the timely adjustment of auxiliary treatment strategies in clinical settings.

Methods: We designed a gas vesicles (GVs)-labeled CAR-T (GVs@CAR-T) complex using nanoscale GVs with excellent ultrasound reflectivity to label CAR-T cells for post-labelling ultrasound molecular imaging.

Clinical characteristics and surgical options of cervical ossification of the posterior longitudinal ligament with trauma: a retrospective comparative study.

Background: Trauma may aggravate the clinical outcome of cervical ossification of the posterior longitudinal ligament (OPLL), but few studies have addressed the optimal surgical strategy for cervical OPLL with trauma. This study aimed to explore the clinical characteristics and discuss surgical options for cervical OPLL with trauma.

Materials And Methods: Enrolled in this study were 262 patients with cervical OPLL who were treated in Shanghai Changzheng hospital between March 2008 and July 2018.

Metformin ameliorates trophoblastic immunometabolic disorders via attenuating TLR4/NF-κB signaling through ATXN7L3-mediated histone H2B monoubiquitination.

Background: Trophoblastic inflammation and glycometabolic reprogramming represent two hallmarks of numerous diverse placental disorders, including but not limited to preterm labor, preeclampsia, and fetal growth restriction. Recent evidence indicates that TLR4/NF-κB signaling mediate the interaction between trophoblastic inflammation and glycometabolism disturbance while pharmacologic doses of metformin (MET, 10 μM) corrected these vicious states via its suppression on this pathway. However, the underlying precise mechanism remain incompletely understood.

Omentin-1 attenuates atrial fibrillation via Src/PI3K/Akt signaling-mediated anti-fibrotic effects in cardiac fibroblasts.

Background: Atrial fibrillation (AF) is characterized by progressive atrial fibrosis, leading to increased morbidity and mortality. While the novel adipokine Omentin-1 demonstrates anti-fibrotic potential across organ systems, its role in AF pathogenesis remains unclear. This study investigates Omentin-1's therapeutic effects and the underlying mechanisms in angiotensin II (Ang II)-induced atrial fibrosis and AF.