Similar coordination environments promote indium-doped MOF-74-Co precatalysts for oxygen evolution reaction.

The sluggish kinetics of the oxygen evolution reaction (OER) necessitate the use of efficient, non-precious metal catalysts. This work reports the rational design of an indium-doped bimetallic metal-organic framework (MOF-74-CoIn) as a precatalyst, achieved through post-synthetic modification of MOF-74-Co. Structural and spectroscopic analyses confirm that In(III) incorporation induces CoO bond contraction while preserves the overall structural integrity.

Energy Deficiency-Induced ATG4B Nuclear Translocation Inhibits PRMT1-Mediated DNA Repair and Promotes Leukemia Progression.

Metabolic alterations and genomic instability are the hallmark features of many cancers. However, the precise mechanisms underlying the intricate links among these processes remain largely unknown. Here, a molecular mechanism is presented that regulates the interplay between cellular energy metabolism and DNA repair.

Pore-Size Effect of Pt Incorporation in MOF-Derived PtNi/C Catalysts for Hydrogen Evolution.

The hydrogen evolution reaction (HER) is a critical step in water electrolysis, holding significant potential for converting intermittent renewable energy into a storable hydrogen fuel. In this work, we synthesized two types of bimetallic PtNi nanoparticles (NPs) embedded in porous metal-organic framework (MOF)-derived carbon nanomaterials, designated as L-PtNi-C and S-PtNi-NC, respectively. In this case, PtCl complex ions cannot be effectively entered into the narrow pores of Ni-ABDC (pore size: 4.

Transferrin receptor 1 (TfR1) functions as an entry receptor for scale drop disease virus to invade the host cell via clathrin-mediated endocytosis.

Scale drop disease virus (SDDV), a distinct member of the genus within the family, has emerged as a novel threat to global teleost aquaculture. Despite its importance, the pathogenic mechanism of SDDV remains largely elusive. In this study, we identified mandarin fish transferrin receptor 1 (TfR1) as an entry receptor for SDDV to invade host cells.

Modeling the vertebrate regulatory sequence landscape by UUATAC-seq and deep learning.

The regulatory sequences of vertebrate genomes remain incompletely understood. To address this, we developed an ultra-throughput, ultra-sensitive single-nucleus assay for transposase-accessible chromatin using sequencing (UUATAC-seq) protocol that enables the construction of chromatin accessibility landscapes for one species in a 1-day experiment. Using UUATAC-seq, we mapped candidate cis-regulatory elements (cCREs) across five representative vertebrate species.

Genetically modified bacterial vesicles with interferon-γ and tumor antigen remodel anti-tumor cellular immunity by modulating dendritic cells.

The tumor vaccine is regarded as a promising immunotherapeutic approach for the treatment of malignant tumors by activating dendritic cell (DC) and eliciting T-cell responses. Toll-like receptor (TLR) ligands are recognized as effective stimulators of DC maturation. Another critical factor in DC maturation is interferon-γ (IFN-γ), which amplifies the TLR signaling pathway and elevates the expression of inflammatory cytokines in DCs.

A high strength multifunctional lignin-based hydrogel dressing with antibacterial, antioxidant, hemostatic and adhesive properties for wound healing.

Developing multifunctional hydrogel wound dressings that integrate hemostasis, adhesion, antibacterial activity, mechanical robustness, and antioxidant properties remains a significant challenge. This study employed both curcumin and lignin to reduce silver ions in situ while simultaneously incorporating Callicarpa nudiflora into an acrylamide-acrylic acid (AM/AA) hydrogel system. Characterization techniques including FTIR, XRD, XPS, SEM, swelling tests, rheological measurements, and mechanical tests revealed that lignin and curcumin synergistically generate quinone free radicals, promoting the self-gelation of the AA/AM system and resulting in the formation of an Ag@Lig-Cur NPs/C.

Detection and Genomic Characterisation of Scale Drop Disease Virus (SDDV) in Farmed Yellowfin Seabream (Acanthopagrus latus) in Southern China.

Scale drop disease virus (SDDV) is a distinct member of the genus Megalocytivirus within the family Iridoviridae, which was recently identified as a causative agent of yellowfin seabream Acanthopagrus latus ascitic diseases (YFSBAD). Here, a molecular surveillance of SDDV in pond-cultured yellowfin seabream was conducted during 2021 to 2023 in Zhuhai, the most significant breeding region for this fish species in China. A total of 73 batches of naturally diseased yellowfin seabream samples were collected for SDDV detection.

Lats1/2 Are Essential for Developmental Vascular Remodeling and Biomechanical Adaptation to Shear Stress.

Background: Mechanical cues exerted by shear stress from blood flow remodel an initial vascular plexus into a ramifying array of large and small vessels. Hemodynamic forces trigger changes in endothelial cell (EC) gene expression and dynamic alterations in cell shape and adhesion. The objective of this study is to elucidate the role of the Lats (large tumor suppressor) 1 and Lats2 (Lats1/2) Hippo pathway kinases in EC transducing of hemodynamic signals as vessels form.

Tumor microenvironment remodeling with a telomere-targeting agent and its cooperative antitumor effects with a nanovaccine.

The nucleoside analogue 6-thio-2'-deoxyguanosine (6-thio-dG, also known as THIO) is a telomere-targeting agent with important clinical potency. It can selectively kill telomerase-positive tumor cells. We previously reported that THIO could successfully induce immunogenic cell death (ICD) in multiple mouse tumor cell lines.

Vitamin C Inhibits Scale Drop Disease Virus Infectivity by Targeting Nrf2 to Reduce Ferroptosis.

Scale drop disease virus (SDDV) poses an escalating threat to global aquaculture, prompting an urgent need for research. Our study found that SDDV infection upregulates genes related to iron, oxidative stress, and lipid metabolism, causing iron overload, reactive oxygen species (ROS) accumulation, and ultimately ferroptosis. Among the tested antioxidants, vitamin C (VC) demonstrated the most potent inhibitory effect in mandarin fish, reducing SDDV-induced mortality by 37.

Scale drop disease virus (SDDV) triggers ferroptosis both in mandarin fish (Siniperca chuatsi) and MFF-1 cells to facilitate virus infection via linking to transferrin receptor 1 (TfR1).

Scale drop disease virus (SDDV) is a distinct member in genus Megalocytivirus of family Iridoviridae, garnering increasing attention due to its significant threat to teleost. Ferroptosis is a new type of cell death discovered recently and involved in various viral infections. Knowledges on SDDV induced ferroptosis remains unclear.

Dissection of the global responses of mandarin fish pyloric cecum to an acute ranavirus (MRV) infection reveals the formation of serositis and then ascites.

Mandarin fish ranavirus (MRV), a new member of the species , sharing over 98% whole-genome nucleotide identity with the well-known largemouth bass virus (LMBV), is a distinct member of the genus within the family . Our recent work showed that acute MRV infection predominantly affects the pyloric cecum, a critical visceral organ in mandarin fish, and was hypothesized to drive the characteristic external clinical sign of severe ascites. In this study, we reveal that acute MRV infection initially targets the serosal layer of the pyloric cecum of mandarin fish, leading to rapid progression into fibrinous serositis characterized by serosal hypertrophy, fibrosis, hyperemia, edema, and tissue adhesions.

Poliovirus shedding after sequential immunization of Sabin-strain inactivated polio vaccines and oral attenuated polio vaccines.

The identification of poliovirus in stool samples post-Oral Poliovirus Vaccine (OPV) immunization is essential for implementing post-eradication monitoring of poliomyelitis. This research presents the inaugural assessment of the virus shedding rate and the genetic diversity of OPV shedding strains across different Inactivated Poliovirus Vaccine (IPV)-OPV sequential immunization schedules. Our findings revealed that the shedding rate of different serotypes in each sequential immunization groups peaked within 7 days following the initial administration of OPV, and then gradually decreased.

Lymphatic Malformations with Activating KRAS Mutations Impair Lymphatic Valve Development Through Matrix Metalloproteinases.

Background: Lymphatic malformations (LMs) are lesions due to inherited or somatic mutations that lead to a defective lymphatic vasculature. Activating KRAS mutations have been identified recently in LM patients with lymphedema, chylous ascites, or life-threatening chylothorax. In a LM mouse model, KRAS mutations are associated with a loss of lymphatic valves, which has been proposed to cause chylothorax via retrograde lymph flow into the pleural space.

Adaptive survival strategies of rumen microbiota with solid diet deficiency in early life cause epithelial mitochondrial dysfunction.

With extreme nutritional substrate deficiency, the adaptive responses of the gastrointestinal microbiota and host metabolism are largely unknown. Here, we successfully established a microbial substrate deficiency model in the rumen without solid diet introduction in neonatal lambs. In the absence of solid diet, we observed a reduction in the Simpson Index of rumen bacteria, along with a marked decline in the abundance of keystone microorganisms such as Prevotella, Selenomonas, Megasphaera, and Succiniclasticum, indicating a simplified microbial interaction network.

Enhancement mechanism and optimization analysis of resonant excitation Laser-induced Breakdown Spectroscopy (LIBS-RE) in gaseous ammonia element detection.

The existing techniques for gas components detection, such as Tunable Diode Laser Absorption Spectroscopy (TDLAS) and Cavity Ring-Down Spectroscopy (CRDS), are constrained by their inability to discern the elemental composition of the gas molecules. As a supplementary technology for detecting the total amount of atoms of each component in a gas, Laser-Induced Breakdown Spectroscopy (LIBS) offers significant advantages in gas elemental composition detection. Nevertheless, there are still some challenges to be overcome, including weak spectral signals and instability.

Pt-Skin Coated PtNi Alloy in Carbon Nanoshells for Enhanced Hydrogen Evolution Activity and Durability.

Hydrogen, as an environmentally sustainable energy carrier, offers substantial potential for addressing the global energy crisis. The development of highly efficient catalysts to accelerate the hydrogen evolution reaction (HER) is critical for the realization of electrochemical hydrogen production via water splitting. Herein, a novel heterogeneous catalyst consisting of PtNi nanoalloys with Pt-enriched surfaces is obtained, which are uniformly distributed within nitrogen-doped hollow carbon nanoshells derived from a complex of Ni-EDTA (ethylene diamine tetraacetate).

SFTSV NSs interacts with AGO2 to regulate the RNAi pathway for viral replication.

RNA interference (RNAi) is a posttranscriptional gene silencing mechanism acting as an antiviral defense in eukaryotes. During viral replication, intermediate double-stranded RNAs are processed into virus-derived small interfering RNAs (vsiRNAs) by the host enzyme, DICER. These vsiRNAs are incorporated into the RNA-induced silencing complex (RISC), where AGO2 cleaves viral genomic RNAs.

Study on an Injectable Chitosan-Lignin/Poloxamer Hydrogel Loaded with Platelet-Rich Plasma for Intrauterine Adhesion Treatment.

It is a great challenge to obtain an ideal hydrogel for the clinical treatment of intrauterine adhesion (IUA) disease. Here, a novel injectable chitosan-lignin/poloxamer hydrogel loaded with platelet-rich plasma (CL-PF127@PRP) was prepared by self-assembly at room temperature. Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), rheological analysis, and injectable writing were used to characterize the structure of the hydrogel.

Multiparameter resting-state functional magnetic resonance imaging as an indicator of neuropsychological changes in Binswanger's disease with mild cognitive impairment.

The underlying neuropathological mechanisms in Binswanger's disease (BD) with mild cognitive impairment (BD-MCI) remain unclear. The multiparameter functional magnetic resonance imaging (fMRI) including amplitude of low-frequency fluctuations (ALFF), fractional amplitude of low-frequency fluctuations (fALFF), regional homogeneity (ReHo), independent component analysis (ICA), and edge-link analysis was utilized to explore the abnormal brain networks of BD-MCI patients. Compared with the BD without MCI group, this study revealed that the ALFF values in the BD-MCI group were significantly increased in the Temporal_Inf_R, Frontal_Mid_Orb_L, and Hippocampus_L, while decreased in the SupraMarginal_R and Precuneus_R.

Preparation and characterization of lauric acid-rich wax oleogel chocolates: Insights into crystallization behavior, storage stability, and bloom resistance.

Coconut oil (CO) and palm kernel oil (PKO), both rich in medium-chain fatty acids (lauric acid), have shown potential as substitutes for cocoa butter (CB). However, their tendency to melt at room temperature limits their application. To overcome this limitation, 8 % natural wax (candelilla) was added to different proportions of CO and PKO to form oleogels, creating a three-dimensional network that enhanced their crystallization behavior and structural stability.

Fluorescence kinetics for upconversion enhancement of Er/Yb doped oxyfluoride glass ceramics by K ions doping.

This study develops an oxyfluoride GCs embedded with β-PbF:4Yb/1Er/xK NCs by conventional melting-quenching method. Based on XRD and EDS line scanning data, it was inferred that Er/Yb and K ions were co-doped in the β-PbF lattice by substituting Pb ions. Compared with K ions free doping GCs, in β-PbF:4Yb/1Er/xK GCs, the synergistic substitution of Pb ions by K and Er/Yb into the β-PbF lattice not only effectively eliminates the charge imbalance defects caused by single doping of Er/Yb ions, but also causes lattice distortion around Er/Yb.

A deep learning framework for screening of anticancer drugs at the single-cell level.

Tumor heterogeneity plays a pivotal role in tumor progression and resistance to clinical treatment. Single-cell RNA sequencing (scRNA-seq) enables us to explore heterogeneity within a cell population and identify rare cell types, thereby improving our design of targeted therapeutic strategies. Here, we use a pan-cancer and pan-tissue single-cell transcriptional landscape to reveal heterogeneous expression patterns within malignant cells, precancerous cells, as well as cancer-associated stromal and endothelial cells.

Tumor Vaccine Exploiting Membranes with Influenza Virus-Induced Immunogenic Cell Death to Decorate Polylactic Coglycolic Acid Nanoparticles.

Immunogenic cell death (ICD) of tumor cells, which is characterized by releasing immunostimulatory "find me" and "eat me" signals, expressing proinflammatory cytokines and providing personalized and broad-spectrum tumor antigens draws increasing attention in developing a tumor vaccine. In this study, we aimed to investigate whether the influenza virus (IAV) is efficient enough to induce ICD in tumor cells and an extra modification of IAV components such as hemeagglutinin (HA) will be helpful for the ICD-induced cells to elicit robust antitumor effects; in addition, to evaluate whether the membrane-engineering polylactic coglycolic acid nanoparticles (PLGA NPs) simulating ICD immune stimulation mechanisms hold the potential to be a promising vaccine candidate, a mouse melanoma cell line (B16-F10 cell) was infected with IAV rescued by the reverse genetic system, and the prepared cells and membrane-modified PLGA NPs were used separately to immunize the melanoma-bearing mice. IAV-infected tumor cells exhibit dying status, releasing high mobility group box-1 (HMGB1) and adenosine triphosphate (ATP), and exposing calreticulin (CRT), IAV hemeagglutinin (HA), and tumor antigens like tyrosinase-related protein 2 (TRP2).