Mammalian cells measure the extracellular matrix area and respond through switching the adhesion state.

Mammalian cells adjust integrin-mediated adhesion based on the composition and structure of the extracellular matrix (ECM). However, how spatially confined ECM ligands regulate cell adhesion initiation remains unclear. Here, we investigate how cells adapt early adhesion to different ECM protein areas.

Cardiovascular Outcomes and Variability in Plasma Lipid Levels Across Body Mass Index Categories: The ARIC Study.

The aim of this study was to investigate associations of cardiovascular outcomes with lipid variability across body mass index categories. We identified 6689 participants (57.1% women) enrolled in the Atherosclerosis Risk In Communities (ARICs) study who had ≥ 3 measurements of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C).

Agxt2l-mediated glycerophospholipid metabolism in trophocytes explains Apis mellifera queen's higher oviposition over A. cerana.

Apis mellifera and Apis cerana are two important honey bee species widely kept and studied. But their queens differ greatly in egg-laying capacity. To determine the mechanisms of this difference, we compare gene expression, chromatin accessibility and spatial localization of differential genes in the ovaries of the two species in virgin queens and laying queens using ATAC-seq, RNA-Seq, homologous gene alignment and spatial transcriptome.

Silicone-Enhanced PAA Supramolecular Hydrogels for High-Toughness Strain Sensors.

Hydrogels have shown significant potential in the field of flexible electronics; however, their practical application remains limited due to mechanical performance constraints. Inspired by the supramolecular interactions observed in nature, this study designs a water-soluble PhAPSi polymer and introduces it into an aqueous acrylic acid solution. During in situ polymerization, a hydrogel network is formed through the self-assembly of supramolecular interactions, resulting in the fabrication of a PAA/PhAPSi hydrogel with both high strength (2.

Integrated visible-light optical coherence tomography and fluorescence scanning laser ophthalmoscopy to image retinal ganglion cell axons.

Retinal ganglion cell (RGC) soma and axonal damage is a hallmark of optic neuropathies. Visible-light OCT fibergraphy (vis-OCTF) enables non-invasive imaging and quantitative assessment of individual RGC axon bundles; however, validating vis-OCTF using confocal fluorescence imaging of flat-mounted postmortem retina is less accurate due to structural alterations caused by flat-mount preparation and cannot be performed longitudinally. For in vivo vis-OCTF validation, we developed an integrated visible-light optical coherence tomography (vis-OCT) and fluorescence scanning laser ophthalmoscopy (SLO) system.

Liyan Kaiyin Formula relieves reflux pharyngitis by regulating M1 macrophage polarization via the NF-κB/NLRP3 pathway.

Background: We aimed to investigate whether Liyan Kaiyin Formula (LYKYF) can relieve reflux pharyngitis in rats by regulating M1 macrophage polarization via the nuclear factor-κB (NF-κB)/Nod-like receptor protein 3 (NLRP3) pathway.

Methods: Forty rats were randomized into a sham group, a laryngopharyngeal reflux disease (LPRD) group, a LYKYF group and a LYKYF+CHPG group (n = 10). Enzyme-linked immunosorbent assay was conducted to measure the serum levels of inflammatory factors interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α).

Therapeutic efficacy of fecal microbiota transplantation in severe food intolerance: a case report.

This report presents the first documented application of fecal microbiota transplantation (FMT) for the management of extensive multi-food intolerance involving 52 specific foods in a pediatric patient with autism spectrum disorder (ASD). A 7 years-old autistic child was diagnosed with food intolerance to 52 items, presenting with generalized rashes, diarrhea, and malnutrition (BMI of 12.9) upon exposure or ingestion of the implicated foods.

Ammonium recovery from wastewater by biochar with different N/O-doped groups and hierarchical pores: Synergistic enhancement mechanisms for strong negative electrostatic potential and micropore filling.

Biochar has shown considerable potential for removing ammonium (NH) from wastewater, supporting sustainable pollution control and nutrient recovery. However, optimizing biochar structure remains challenging, especially concerning the interplay between pore structure, surface functional groups, and NH adsorption mechanisms. This study addresses these challenges by investigating the adsorption mechanism of NH on biochar with nitrogen (N)- and oxygen (O)-doped groups, as well as hierarchical pores, through density functional theory (DFT) calculations and molecular dynamics (MD) simulations.

Investigating the status and influencing factors of professional identity formation among Chinese medical interns: a mixed methods study.

Background: Professional Identity Formation (PIF) involves a movement through a series of conceptually distinct developmental stages. Kegan's theory of PIF is divided into 6 phases (stages 0-5). It is believed that students undergo stages 2-4 (imperial, interpersonal, institutional) during medical education.

Construction of MXene-Based Heterostructured Hybrid Separators for Lithium-Sulfur Batteries.

The advancement of lithium-sulfur (Li-S) batteries has been hindered by the shuttle effect of lithium polysulfides (LiPSs) and sluggish redox kinetics. The engineering of functional hybrid separators is a relatively simple and effective coping strategy. Layered transition-metal carbides, nitrides, and carbonitrides, a class of emerging two-dimensional materials termed MXenes, have gained popularity as catalytic materials for Li-S batteries due to their metallic conductivity, tunable surface chemistry, and terminal groups.

Dynamic Behavior of Ti/Ti Single-Lap Laminated Structure with a Large-Diameter Bolt-Based Electromagnetic Force: Numerical Simulation and Experimental Verification.

Electromagnetic force installation is recognized as a viable solution for interference-fit issues in large-diameter bolts. However, the dynamic mechanical behavior of the joint during installation has not been fully clarified. This study investigated the dynamic mechanical behavior of large-diameter Ti/Ti interference-fit bolted joints during electromagnetic installation through numerical simulation and experimental validation.

Research on the preference of community health communication service utilization by patients with cardiovascular diseases in China.

Objectives: This study sought to examine cardiovascular patients' preferences for community health communication and to offer recommendations to boost the engagement with health communication programs.

Methods: We conducted a discrete choice experiment involving 914 cardiovascular patients, assessing their preferences across five attributes: Health information communicator, Health information communication content, Health information communication channel, Health information form, and Cost willing to pay each time. The data were analyzed using a mixed logit model to account for preference heterogeneity.

The addition of alpha-ketoglutarate to NT-proBNP improves the prediction of long-term all-cause mortality in acute heart failure patients.

Background And Objective: Alpha-ketoglutarate (AKG), is a major intermediate metabolite of the tricarboxylic acid cycle, and is closely associated with cardiometabolic disease prognosis. Previous studies indicated that AKG is related to myocardial energy expenditure levels and reflects adverse short-term outcomes in heart failure (HF) patients. In this prospective cohort study, we examined the long-term prognostic value of AKG levels in acute HF (AHF) patients.

Morphological and metabolic changes in Changshan Huyou (Citrus changshan-huyou) following natural tetraploidization.

Background: Polyploids in citrus are generally used to improve crop varieties. Changshan Huyou (Citrus changshan-huyou) is a native citrus species in China that is highly adaptable and has pharmaceutical value. However, the influence in Changshan Huyou following polyploidization remains unclear.

Trimethylamine N-oxide aggravates human aortic valve interstitial cell inflammation by regulating the macrophages polarization through a N6-methyladenosine-mediated pathway.

Background: Trimethylamine N-oxide (TMAO) is a gut microbial metabolite that promotes calcified aortic valve disease (CAVD), but the underlying mechanism remains obscure. Herein, we aim to test the hypothesis that TMAO regulated the inflammatory process in aortic valves via N6-methyladenosine (m6A) RNA methylation-mediated macrophage polarization.

Methods: In vitro, we stimulated macrophages (Phorbol-12-Myristate-13-Acetate-induced THP-1) with TMAO and assessed the expression of methyltransferase-like 3 (Mettl3), IL-1 receptor associated kinase M (IRAK-M) and polarization markers.

The Relationship Between Mental Health Literacy and Social Well-Being: A Longitudinal Study in China.

In this study, 793 college students were examined through the utilization of the socioeconomic status scale, mental health literacy scale, and social well-being questionnaire at T1 and T2, respectively, with the aim of exploring the relationship between mental health literacy and social well-being and the relative static and dynamic development of the two. The results indicated that mental health literacy was significantly and positively correlated with social well-being to a moderate extent (T1: = 0.31; T2: = 0.

PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction.

Introduction: Heart failure with preserved ejection fraction (HFpEF) is a complex condition characterized by metabolic dysfunction and myocardial lipotoxicity. The roles of PTEN-induced kinase 1 (PINK1) and peroxiredoxin-2 (Prdx2) in HFpEF pathogenesis remain unclear.

Objective: This study aimed to investigate the interaction between PINK1 and Prdx2 to mitigate cardiac diastolic dysfunction in HFpEF.

Indocyanine Green Fluorescence Plus Blue Dye for Sentinel Lymph Node Biopsy in Patients Undergoing Neoadjuvant Chemotherapy for Breast Cancer: A Multicenter, Prospective Cohort Study.

Background: Sentinel lymph node biopsy (SLNB) using radioisotope tracer plus blue dye is the gold standard after neoadjuvant chemotherapy (NAC) in initially cN1 breast cancer patients, but clinical use still has limitations. This study aims to examine diagnostic performance of dual indocyanine green (ICG) and methylene blue tracing for SLNB in patients who have completed NAC for breast cancer with initially cN1 disease.

Methods: Adult women (20-80 years of age) scheduled to undergo NAC for biopsy-proven cT0-3N1M0 primary invasive breast cancer were consecutively enrolled in this prospective, multicenter, cohort study.

PINK1-mediated mitophagy attenuates pathological cardiac hypertrophy by suppressing the mtDNA release-activated cGAS-STING pathway.

Aims: Sterile inflammation is implicated in the development of heart failure (HF). Mitochondria play important roles in triggering and maintaining inflammation. Mitophagy is important for regulation of mitochondrial quality and maintenance of cardiac function under pressure overload.

A meta-analysis of conservation tillage management effects on soil organic carbon sequestration and soil greenhouse gas flux.

Conservation tillage practices, including reduced tillage (RT), no-tillage (NT) and straw return (SR), have been widely adopted to enhance soil organic carbon density (PSOC) and improve the soil quality while mitigating the negative environmental impacts of intensive farming. However, current studies on the effects of these practices on SOC sequestration and NO flux show considerable variability, making it challenging to draw definitive conclusions about the individual and combined impacts of conservation tillage practices and introducing substantial uncertainty in estimating the agricultural sector's potential to mitigate climate change. To address this gap, we conducted a meta-analysis of 902 pairwise comparisons from 90 peer-reviewed publications to evaluate the effects of five conservation tillage practices (straw return (SR), reduced tillage (RT), no-tillage (NT), straw return combined with tillage reduction (SR + RT) and straw returning combined with no-tillage (SR + NT)) on C sequestration and NO emissions from agricultural soils.

Vericiguat attenuates doxorubicin-induced cardiotoxicity through the PRKG1/PINK1/STING axis.

Doxorubicin (DOX) is restricted due to its severe cardiotoxicity. There is still a lack of viable and effective drugs to prevent or treat DOX-induced cardiotoxicity(DIC). Vericiguat is widely used to treat heart failure with reduced ejection fraction.

Research progress on the correlation between cataract occurrence and nutrition.

Cataract is a common eye disease characterized by lens opacity, leading to blurred vision and progressive blindness of the eye. Factors affecting the development of cataracts include nutrition, oxidative stress, micronutrients and inflammatory factors, and also include genetics, toxicity, infrared exposure, hyperuricemia, and mechanical injuries. Among the nutritional factors, a balanced diet, vegetarian diet, dairy products and vegetables are protective against cataracts; high-sodium diet, high intake of carbohydrates and polyunsaturated fatty acids may increase the risk of cataracts; and increased intake of proteins, especially animal proteins, may prevent nuclear cataracts.

The Honey Bee Colony's Criterion for Candidate Selection: "Ongoing" or "One-Shot"?

In the honey bee, the queen's death severely threatens the survival of the colony. In an emergency, new queens are reared from young worker larvae, where nepotism is thought to influence the choice of queen candidates by the workers. This article simulates the emergency queen-rearing process in a colony under natural conditions and records the results of colony selection (without nepotism).