CSLD5-mediated cell wall remodelling regulates tissue mechanics and shoot meristem growth.

New tissues and organs in plants develop from stem cells located in meristematic tissues. Cell wall-mediated mechanics has been proposed to play crucial roles in controlling stem cell activity. Here, we show that in Arabidopsis shoot apical meristems (SAMs) Cellulose Synthase Like-D5 (CSLD5)-mediated cell wall synthesis modulates tissue mechanics.

Sub-Nano Ir-Based Alloy Clusters by Hierarchical Confinement Effect for Water Splitting.

The synthesis of sub-nanoscale noble metal catalysts is pivotal for enhancing electrocatalytic performance, yet achieving precise control over particle size at this scale remains a critical challenge. In this work, we propose a hierarchical confinement strategy which combines spatial confinement at nanoscale and anchoring confinement at atomic scale, to overcome the size limitations imposed by high-temperature sintering. Using this strategy, a series of uniformly sized (∼1 nm) Ir-based alloy clusters, including IrMn, IrFe, IrCo and IrNi, are successfully fabricated.

Hsa_piR_016975 Is a Novel Target of Nanotherapy that Boosts Hepatoma Progression and Sorafenib Resistance by Abating Maspin/GPX4-Mediated Ferroptosis.

PIWI-interacting RNAs (piRNAs) are known to be involved in germline development, but their potential mechanisms in carcinogenesis remain elusive. Herein, we investigated the roles of hsa_piR_016975, a novel piRNA, in hepatocellular carcinoma (HCC) progression and its therapeutic effects on drug resistance to sorafenib. The results disclosed that hsa_piR_016975 was highly expressed in HCC and promoted HCC growth, metastasis, epithelial mesenchymal transition (EMT) formation, and sorafenib resistance.

Magnetic nanobead assisted the dual targets driven fluorescent biosensor based on SPEXPAR and MNAzyme for the olfactory marker protein detection.

Fig. 1: Schematic illustration of the principle of the Dual targets driven SPEXPAR assisted MNAzyme (D-S-M) fluorescent biosensor for olfactory marker protein (OMP) detection. A.

Semimetal-triggered covalent interaction in Pt-based intermetallics for fuel-cell electrocatalysis.

Platinum-based intermetallic compounds (IMCs) play a vital role as electrocatalysts in a range of energy and environmental technologies, such as proton exchange membrane fuel cells. However, the synthesis of IMCs necessitates recombination of ordered Pt-M metallic bonds with high temperature driving, which is generally accompanied by side effects for catalysts' structure and performance. In this work, we highlight that semimetal atoms can trigger covalent interactions to break the synthesis-temperature limitation of platinum-based intermetallic compounds and benefit fuel-cell electrocatalysis.

Stabilized ε-FeC catalyst with Mn tuning to suppress C1 byproduct selectivity for high-temperature olefin synthesis.

Accurately controlling the product selectivity in syngas conversion, especially increasing the olefin selectivity while minimizing C1 byproducts, remains a significant challenge. Epsilon FeC is deemed a promising candidate catalyst due to its inherently low CO selectivity, but its use is hindered by its poor high-temperature stability. Herein, we report the successful synthesis of highly stable ε-FeC through a N-induced strategy utilizing pyrolysis of Prussian blue analogs (PBAs).

A Redox Flow Battery-Integrated Rechargeable H/O Fuel Cell.

The practical application of the H/O proton-exchange membrane fuel cell (PEMFC) is being greatly limited by the use of high-cost Pt as electrode catalysts. Furthermore, the H/O PEMFC is nonrechargeable and thus precludes kinetics energy recovery when equipped on electric vehicles and peak power regulation when combined to power grids. Here, we demonstrate a rechargeable H/O PEMFC through embedding a redox flow battery into a conventional H/O PEMFC.

Symmetry-Induced Regulation of Pt Strain Derived from Pt Ga Intermetallic for Boosting Oxygen Reduction Reaction.

Pt-based fuel cell catalysts with excellent activity and stability for proton-exchange membrane fuel cells (PEMFCs) have been developed through strain regulation in recent years. Herein, this work demonstrates that symmetry-induced strain regulation of Pt surface of PtGa intermetallic compounds can greatly enhance the catalytic performance of the oxygen reduction reaction (ORR). With the strain environment varies derived from the lattice mismatch of analogous PtGa core but different symmetry, the Pt surface of the PtGa alloy and the Pt Ga (Pm m) precisely realize 0.

Avoiding threats, but not acquiring benefits, explains the effect of future time perspective on promoting health behavior.

Promoting health behavior among the public is always a pressing issue. The present research systematically investigated the association between future time perspective and adherence to health behavior including dietary habits, physical activities, and substance use in a Chinese college student sample ( = 519). Results showed that individuals with stronger future time perspective were more likely to adhere to health behavior.

Endovascular Stent Implantation in the Treatment of Vertebrobasilar Dolichoectasia.

Vertebrobasilar dolichoectasia (VBD) is a rare disease in clinic, with an incidence of 0.06% and 5.8%.

Robot-Guided Stereotactic Puncture and Drainage in the Treatment of Thalamic Abscess.

Brain abscess is rare in clinic, the reported incidence is only 0.4 to 0.90 per 100,000 population, and most of them have a history of prodromal infection.

Surface Anion Promotes Pt Electrocatalysts with High CO Tolerance in Fuel-Cell Performance.

Platinum reaches considerable activity and stability as an electrocatalyst but is not always capable of maintaining such performance under CO poisoning, particularly in CO residual fuels for practical proton-exchange membrane fuel cells (PEMFCs). In this work, we report that surface anions including a series of nonmetal elements on Pt nanoparticles result in outstanding CO tolerance for electrocatalysts in fuel cells. In particular, phosphorus surface-anion-modified Pt (denoted as P-Pt) possesses more than 10-fold enhancement of CO tolerance (only 8.

Case report: Cavernous hemangioma in the right frontoparietal junction.

Background: Primary intraosseous cavernous hemangioma is a benign tumor with slow growth and is rarely seen in clinics. The clinical manifestations of most patients are progressive enlargement of the head mass.

Case Presentation: We report a 30-year-old female patient with cavernous hemangioma at the frontoparietal junction.

A comparative analysis of differentially expressed genes in rostral and caudal regions after spinal cord injury in rats.

The initial mechanical damage of a spinal cord injury (SCI) triggers a progressive secondary injury cascade, which is a complicated process integrating multiple systems and cells. It is crucial to explore the molecular and biological process alterations that occur after SCI for therapy development. The differences between the rostral and caudal regions around an SCI lesion have received little attention.

Anti-inflammatory Activity of a Polypeptide Fraction From in Amyloid β Oligomers Induced Model of Alzheimer's Disease.

Neuroinflammation plays a crucial role in neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD), and anti-inflammation has been considered as a potential therapeutic strategy. polypeptide fraction k (ABPPk) was shown to protect neurons from death and suppress microglia and astrocyte activation in PD model mice. However, how ABPPk regulates neuroinflammation to exert a neuroprotective role remains unclear.

Loc680254 regulates Schwann cell proliferation through Psrc1 and Ska1 as a microRNA sponge following sciatic nerve injury.

Peripheral nerve injury triggers sequential phenotype alterations in Schwann cells, which are critical for axonal regeneration. Long noncoding RNAs (lncRNAs) are long transcripts without obvious coding potential. It has been reported that lncRNAs participate in diverse biological processes and diseases.

Revascularization After Traumatic Spinal Cord Injury.

Traumatic spinal cord injury (SCI) is a complex pathological process. The initial mechanical damage is followed by a progressive secondary injury cascade. The injury ruptures the local microvasculature and disturbs blood-spinal cord barriers, exacerbating inflammation and tissue damage.

Genetic Dissection of Growth Traits in a Unique Chicken Advanced Intercross Line.

The advanced intercross line (AIL) that is created by successive generations of pseudo-random mating after the F generation is a valuable resource, especially in agricultural livestock and poultry species, because it improves the precision of quantitative trait loci (QTL) mapping compared with traditional association populations by introducing more recombination events. The growth traits of broilers have significant economic value in the chicken industry, and many QTLs affecting growth traits have been identified, especially on chromosomes 1, 4, and 27, albeit with large confidence intervals that potentially contain dozens of genes. To promote a better understanding of the underlying genetic architecture of growth trait differences, specifically body weight and bone development, in this study, we report a nine-generation AIL derived from two divergent outbred lines: High Quality chicken Line A (HQLA) and Huiyang Bearded (HB) chicken.

Multiple ancestral haplotypes harboring regulatory mutations cumulatively contribute to a QTL affecting chicken growth traits.

In depth studies of quantitative trait loci (QTL) can provide insights to the genetic architectures of complex traits. A major effect QTL at the distal end of chicken chromosome 1 has been associated with growth traits in multiple populations. This locus was fine-mapped in a fifteen-generation chicken advanced intercross population including 1119 birds and explored in further detail using 222 sequenced genomes from 10 high/low body weight chicken stocks.

Food intake-related genes in chicken determined through combinatorial genome-wide association study and transcriptome analysis.

The chicken gizzard is the primary digestive and absorptive organ regulating food intake and metabolism. Body weight is a typical complex trait regulated by an interactive polygene network which is under the control of an interacting network of polygenes. To simplify these genotype-phenotype associations, the gizzard is a suitable target organ to preliminarily explore the mechanism underlying the regulation of chicken growth through controlled food intake.

Development and characterization of marker-free and transgene insertion site-defined transgenic wheat with improved grain storability and fatty acid content.

Development of marker-free and transgene insertion site-defined (MFTID) transgenic plants is essential for safe application of transgenic crops. However, MFTID plants have not been reported for wheat (Triticum aestivum). Here, we prepared a RNAi cassette for suppressing lipoxygenase (LOX) gene expression in wheat grains using a double right border T-DNA vector.

Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits.

GW2 is emerging as a key genetic determinant of grain weight in cereal crops; it has three homoeologs (TaGW2-A1, -B1 and -D1) in hexaploid common wheat (Triticum aestivum L.). Here, by analyzing the gene editing mutants that lack one (B1 or D1), two (B1 and D1) or all three (A1, B1 and D1) homoeologs of TaGW2, several insights are gained into the functions of TaGW2-B1 and -D1 in common wheat grain traits.

High expression of LAMP1 as a prognostic marker in patients with epithelial ovarian cancer.

Lysosome Associated Membrane Protein-1 (LAMP1), expressed in several functional processes, is a heavily glycosylated lysosomal membrane protein which is able to protect the lysosomal membranes from intracellular proteolysis. Its pro-tumorigenic effects are involved in the development of several types of Malignancy. However, the role of LAMP1 in human Epithelial Ovarian Cancer (EOC) patients remains unclear.

Isolation of rat Schwann cells based on cell sorting.

The present study presented a protocol that can be used to obtain rapidly a high purity of proliferating rat Schwann cells from freshly dissociated rat peripheral nerves. The sciatic nerves of newborn rats (1‑3 day old) were dissociated, and the Schwann cells (SCs) were purified using fluorescence‑activated cell sorting (FACS) based on the SC membrane‑specific expression of the low‑affinity nerve growth factor receptor, p75NGFR and oligodendrocyte marker 4. Following sorting, the cells were plated on poly‑l‑lysine‑coated dishes in SC culture medium containing DMEM with 10% FBS, 1% penicillin/streptomycin, 2 µM forskolin and 10 ng/ml HRG.

Optimized double-digest genotyping by sequencing (ddGBS) method with high-density SNP markers and high genotyping accuracy for chickens.

High-density single nucleotide polymorphism (SNP) markers are crucial to improve the resolution and accuracy of genome-wide association study (GWAS) and genomic selection (GS). Numerous approaches, including whole genome sequencing, genome sampling sequencing, and SNP chips are able to discover or genotype markers at different densities and costs. Achieving an optimal balance between sequencing resolution and budgets, especially in large-scale population genetics research, constitutes a major challenge.