Screening of cellulose-degrading bacteria and its degradation and growth-promoting applications.

More than one billion tons of chaff waste are generated globally every year, but traditional recycling methods face the dual challenges of inefficiency and environmental risks, to solve this problem, this study innovatively achieves the dual functions of lignocellulosic synergistic degradation and plant promotion by constructing synthetic microbial communities. Firstly, a cellulose-degrading bacterium cmk-7 (Chromobacterium violaceum) was screened from soil based on Congo red staining method, and the maximum values of CMCase enzyme activity and FPase enzyme activity were 289.12 U/mL and 332.

A light-controlled one-tube detection platform combining CRISPR-Cas12a and RPA: an innovative approach for rapid diagnosis of .

Background: is a significant pathogen associated with nosocomial infections, predominantly affecting immunocompromised patients, and is linked to high mortality rates. To control infection rates, there is an urgent need for a diagnostic method that is cost-effective, rapid, and user-friendly, meeting the current demand for timely diagnosis.

Methods: We have developed a one-tube detection method for UV light unlocking based on RPA-CRISPR/Cas12a technology.

Plasma hydrogen sulfide as a promising predictor of collateral cerebral circulation in acute ischemic stroke.

Objectives: To investigate the relationship between plasma hydrogen sulfide (HS) levels and collateral circulation in patients with acute ischemic stroke (AIS), and to explore the effects of vascular endothelial growth factor (VEGF), homocysteine (Hcy), folic acid (FA), vitamin B (VB), and vitamin B (VB).

Methods: A total of 68 AIS patients were enrolled and classified into two groups based on collateral vessel grading: the Good Collateral Circulation (GCC) group (n = 37) and the Poor Collateral Circulation (PCC) group (n = 31). Plasma levels of HS, VEGF, Hcy, FA, VB, and VB were measured on the 2 and 7 days after admission using microassays and ELISA.

Optical characteristics and formation mechanism of haze events in Qingdao, a coastal city in China.

During the field campaign conducted in Qingdao, Shandong Province, from December 2019 to January 2020, six significant haze episodes occurred. To investigate the optical characteristics and formation mechanisms of haze in this region, we utilized an optical particle counter to measure the size distribution of aerosol particles. The results showed that the average Angström exponent value during the campaign was 1.

Rapid detection of by glycerol one-pot RAA/CRISPR-Cas12a method.

(PA), an opportunistic pathogen commonly responsible for hospital-acquired infections, poses significant threats to human health. To enable rapid and reliable PA detection while effectively mitigating aerosol contamination risks inherent in conventional methods. We developed a glycerol one-pot Recombinase-aided Amplification (RAA)/CRISPR-Cas12a method.

Mining-induced disaster mechanism and stress adjustment technology of deep buried weakly cemented overburden.

Under the typical condition of deep buried weakly cemented overburden, the mining roadway is affected by strong mining stress and high ground stress, its stress environment is complicated, and the disaster behaviors are severe. In this study, the mining disaster behaviors and stress distribution characteristics under this condition are obtained by taking No. 130608 return air roadway of a deep buried weakly cemented overburden mine as the engineering background.

Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed rats.

Aim: To explore the effects of arabinoxylan on the BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex of post-stroke depressed rats, and to explore its neuronal protective effects through the microbial-gut-brain axis in the regulation of this pathway.

Methods: The rat model of post-stroke depression (PSD) was established by middle cerebral artery occlusion (MCAO) combined with chronic unpredictable mild stimulation (CUMS). They were randomly divided into 5 groups (blank control, post-stroke depression, arabinoxylan, fluoxetine hydrochloride, fluoxetine hydrochloride combined arabinoxylan).

Structural importance analysis and key chambers identification for chain instability of chamber group in coal mine.

Accurately locating the weak links in chamber group is crucial for achieving targeted and directional control for its surrounding rock. In this paper, the topological structure of the chamber group was first established, which divided the chambers into important chambers and general chambers. The chain instability inertia I was defined as the ratio of important chambers m to all chambers n, which can reflect the difficulty of chain instability for chamber group.

Inetetamab triggers cardiotoxicity through its interaction with apoptosis, oxidative stress and autophagy pathways.

Inetetamab, a trastuzumab biosimilar, has obtained approval for the treatment of HER2-positive advanced metastatic breast cancer. Despite studies indicating its cardiotoxic properties, the intricate mechanisms underlying its toxicity remain elusive. In the present study, we demonstrated in vitro that exogenous administration of Inetetamab triggered injury in H9c2 myocardial cells, characterized by a significant decrease in cell viability and noticeable morphological changes.

Gut microbiota in liver diseases: initiation, development and therapy.

The gut microbiota plays a pivotal role in the pathogenesis and progression of various liver diseases, including viral hepatitis, alcoholic fatty liver disease, metabolic dysfunction-associated steatotic liver disease, drug-induced hepatitis, liver cirrhosis, hepatocellular carcinoma, and other hepatic disorders. Research indicates that dysbiosis of the gut microbiota can disrupt the integrity of the intestinal barrier and interfere with the immune functions of the gut-liver axis, thereby mediating the progression of liver diseases. Analysis of microbial composition and metabolites in fecal samples can assess the diversity of gut microbiota and the abundance of specific microbial populations, providing auxiliary diagnostic information for liver diseases.

Parametric modeling of mixed-layer turbulent structures based on sounding data.

This study systematically investigates the vertical characteristics of optical turbulence in the atmospheric boundary layer using high-resolution radiosonde data from three regions in China: the Shandong Peninsula (Site A), eastern Zhejiang (Site B), and southern Hainan (Site C). A parameterization model of the mixed-layer turbulence structure was developed, with a particular focus on the impact of vertical resolution on the estimation of the atmospheric refractive index structure constant (2). By comparing models constructed at 10 m, 50 m, and 100 m resolutions, the robustness of the exponential decay model was validated.

Geospatially enhanced convolutional neural network for HY-2B scatterometer-based marine wind field retrieval.

Precise monitoring of sea surface wind fields is imperative for understanding ocean-atmosphere interactions and extreme weather evolution, where spaceborne scatterometers provide unique observational capabilities but face inherent limitations in accuracy and efficiency using the geophysical model function (GMF). We developed convolutional neural network-based models (CNN-WSPD and CNN-WDIR respectively for sea surface wind speed and direction retrievals) integrating HY-2B scatterometer data and ECMWF reanalysis with geographical coordinates. The innovation lies in embedding positional features into multi-angle backscatter processing, enhancing physical consistency across diverse marine environments.

Procedural outcomes of transvenous lead extraction in patients with abandoned leads: experience from a large single centre.

Aims: With the rapid growth of cardiac implantable electronic device (CIED) implantations, lead management has become increasingly challenging. Transvenous lead extraction (TLE) is now a primary approach for addressing lead-related complications. However, data on TLE in patients with abandoned leads remain limited.

Comparisons of sediment mass accumulation rates in different regions of Dongting Lake and their environmental indications: Based on Pu, Cs, and Pb.

In this study, 24 sediment cores collected from Dongting Lake were detected by inductively coupled plasma-mass spectrometry and HPGe γ-spectrometer for Pu, Cs, Ra, and Pb, respectively. Based on the vertical profiles of Pb, Pu, and Cs, sediment mass accumulation rates (MAR) were calculated, and the responses to major environmental events and anthropogenic activities were discussed. The vertical profiles of Cs and Pu exhibit bimodal distribution, the secondary peaks most probably indicated the fallout from a series of Chinese nuclear tests (CNT) during the 1970s.

Molecular mechanisms of MAPK9, BAX, and TFEB proteins: Genetic correlations between oxidative stress and autophagy pathways in Alzheimer's disease.

Alzheimer's disease (AD) is a complex neurodegenerative disease whose pathological mechanisms involve dysregulation of oxidative stress and autophagy pathways. MAPK9, BAX and TFEB were used as key proteins. Wayne analysis was used to identify genes associated with autophagy and oxidative stress, and protein-protein interaction (PPI) networks were constructed to study the associations between key genes.

No difference in outcomes with early vs late antibiotic prophylaxis for term premature rupture of membranes: a multicenter analysis.

Background: Although guidelines suggest administering antibiotics 12 to 18 hours after the rupture of membranes in women with term premature rupture of membranes, in practice, clinicians tend to initiate prophylactic antibiotics as soon as possible to avoid risk of infection.

Objective: This study aimed to assess whether early administration of prophylactic antibiotics for term premature rupture of membranes reduces the incidence of maternal and neonatal infections.

Study Design: This multicenter prospective cohort study included women with term premature rupture of membranes.

Soil microbial carbon and nitrogen limitation constraints soil organic carbon stability in arid and semi-arid grasslands.

Microorganisms play dual roles in soil organic carbon (SOC) decomposition and accumulation. Despite advancing insights into their involvement in the carbon cycle, understanding the impact of microbial community structure and physiological traits on SOC stabilization in arid and semi-arid grasslands remains elusive. Here, we analyzed arid and semi-arid grasslands SOC stability by comparing the ratio of mineral-associated organic carbon (MAOC) to particulate organic carbon (POC) across a grassland transect in north-south Ningxia, encompassing various grassland types and a broad climatic gradient (ΔMAP = 450 mm).

Differential analysis of microRNAs in plasma exosomes in patients with cerebral ischemic stroke.

The high incidence, disability, mortality, and recurrence rates of cerebral infarction impose a heavy burden on both the Chinese and global populations. It is essential for the early diagnosis, prevention, and protection against brain cell injury. To identify differentially expressed microRNAs (miRNAs) in plasma exosomes of patients with cerebral ischemic stroke, determine relevant biomarkers, and explore their potential signaling pathways.

The promotive effect of Caspase-11 overexpression in a rat model of chronic kidney disease and the therapeutic efficacy of exosome-delivered siRNA in inhibiting Caspase-11.

This study investigates the role of Caspase-11 in Chronic Kidney Disease (CKD) and examines the therapeutic potential of inhibiting Caspase-11 using exosome-mediated siRNA. We established a CKD rat model and analyzed the expression of Caspase-11 through immunohistochemistry. The study involved overexpressing Caspase-11 using an adeno-associated virus (AAV) and constructing exosomes loaded with siRNA targeting Caspase-11 (exo-si-Caspase-11).

Effect of acupuncture on quality of life in atrial fibrillation: study protocol for a randomised controlled trial.

Introduction: Atrial fibrillation (AF) is the prevalent cardiac arrhythmia and can significantly impair the quality of life (QoL). Although catheter ablation (CA) is an established treatment for AF,post-procedural complications or perceived inadequate control of AF may diminish the QoL for some patients, potentially even to levels lower than pre-procedure. Preliminary findings from our previous pilot trial indicate that acupuncture may positively influence QoL in AF patients post-CA.

Establishment of a platform based on dual RPA combined with CRISPR/Cas12a for the detection of and its KPC resistance gene.

Carbapenem resistant (CRKP) can cause serious hospital- and community-acquired infections. Treatment for CRKP infection is limited, resulting in prolonged hospitalization and high consultation costs. The KPC genotype has the highest detection rate of CRKP, and its mortality rate is higher than the overall mortality rate of CRKP.