Publications by authors named "Shaoping Long"
A rapid and ultra-sensitive dual readout platform for detection based on RPA-CRISPR/Cas12a.
Front Cell Infect Microbiol
July 2024
Article Synopsis
- The study focuses on a fast and convenient method for detecting a specific hospital-acquired bacterium, which conventional methods struggle with.
- It utilizes a combination of Recombinase Polymerase Amplification (RPA) and CRISPR technology to enhance the detection signal and accuracy.
- The new platform showed high sensitivity and specificity, successfully identifying the bacterium in clinical samples and proving to be a rapid and effective diagnostic tool.
was one of the most common bacteria that caused foodborne illness, with () and () infections accounting for more than 75% of human salmonella infections. In this study, we developed a method of dual recombinase polymerase amplification (RPA) combined with a lateral flow dipstick for the rapid detection of and in clinical specimens (stool). The entire reaction process, including amplification and result reading, could be completed within 65 min.
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