Rapid detection of by glycerol one-pot RAA/CRISPR-Cas12a method.

(PA), an opportunistic pathogen commonly responsible for hospital-acquired infections, poses significant threats to human health. To enable rapid and reliable PA detection while effectively mitigating aerosol contamination risks inherent in conventional methods. We developed a glycerol one-pot Recombinase-aided Amplification (RAA)/CRISPR-Cas12a method.

Insights into the disinfection byproduct bromochloroacetamide-induced cardiotoxicity of zebrafish embryo-larvae: A multiomics approach and comparison of biomarker responsiveness.

Bromochloroacetamide (BCAcAm), an inevitable byproduct of the water treatment disinfection process, is widely detected in drinking water. Previous toxicological and in silico results suggested that developmental effects are associated with analogous chemical exposure; however, the key molecular events and underlying mechanisms remain unclear, especially in the early stages of aquatic organisms. In the present study, a zebrafish larval model was used to comprehensively assess the developmental toxicity of BCAcAm via transcriptional, metabolic, biochemical and morphological tests.

Decrease in cystathionine-β-synthase in tendon tissue is an independent risk factor for rotator cuff retear following arthroscopic repair.

Background: Rotator cuff retear is a common complication after arthroscopic repair, and identifying its risk factors is crucial for improving treatment outcomes. The lack of hydrogen sulfide (HS) in the degenerative rotator cuff tendon may inhibit normal tendon-to-bone healing, as HS is a key regulator for stem cells to differentiate into chondrocytes rather than fibroblasts. This study was designed to investigate whether the expression level of HS synthases correlates with rotator cuff retear.

FEED8-Accelerated Biotransformation of Hydroxycinnamic Acids and Anthocyanins into Hydroxyphenyl-Type Pyranoanthocyanins and Characterization of Their Stability and Antioxidant Capacity.

Hydroxyphenyl-type pyranoanthocyanins are anthocyanin-derived pigments with a vivid and stable color and biological activity, making them natural food colorants with potential applications. In this study, hydroxyphenyl-type pyranoanthocyanins, cyanidin-3-glucoside-4-vinylcatechol (C3G_VC) and cyanidin-3-glucoside-4-vinylphenol (C3G_VP), were efficiently prepared by the biotransformation of caffeic acid and p-coumaric acid with cyanidin-3-glucoside (C3G) using FEED8 (FEED8) fermentation at 37 °C in a model system (pH = 3.5).

Increased inflammation burden index increasing the risk of poor prognosis in patients with chronic kidney disease in NHANES study.

Objective: Several studies illustrated high level of inflammation is an important factor affecting the poor prognosis of patients with chronic kidney disease. Inflammatory burden index (IBI) is a composite maker by incorporating a variety of inflammatory markers to facilitate more in-depth assessment for systemic inflammation. The purpose of this research is to examine the correlation between IBI and prognosis in CKD population of American adults.

Hybrid framework for improved PM2.5 prediction based on seasonal-trend decomposition and tailored component processing.

Precise prediction of PM2.5 concentrations is crucial for effective environmental management and the safeguarding of public health. However, accurately forecasting PM2.

"Dual lock-and-key" triggered and endoplasmic reticulum targeting nanophotosensitizers for activatable Type-I photodynamic and photothermal therapies.

Photodynamic therapy (PDT) has emerged as a critical modality in cancer treatment with the merits of non-invasiveness, spatiotemporal control, and minimal drug resistance. However, the clinical application of PDT is often hindered by inherent limitations, including side effects caused by the "always on" state of reactive oxygen species (ROS) and low ROS generation efficiency in hypoxic tumors. To overcome these limitations, we developed a tumor microenvironment (TME) "dual lock-and-key" triggered and endoplasmic reticulum (ER) targeting nanophotosensitizer for fluorescence imaging-guided activatable Type-I PDT and photothermal therapy (PTT).

Recent progress in macrophage-mediated tendon injury and healing.

Recently, the role of macrophages in tendon repair has received increased attention. These cells are versatile, playing multiple roles, such as defending the host, engulfing debris, producing growth factors, and releasing substances that can both promote and alleviate inflammation. Within the scope of tendon repair and tendinopathy resolution, macrophages are essential contributors.

Solution structure and synaptic analyses reveal determinants of bispecific T cell engager potency.

Bispecific T cell engagers (TcEs) link T cell receptors to tumor-associated antigens on cancer cells, forming cytotoxic immunological synapses (IS). Close membrane-to-membrane contact (≤13 nm) has been proposed as a key mechanism of TcE function. To investigate this and identify potential additional mechanisms, we compared four immunoglobulin G1-based (IgG1) TcE Formats (A-D) targeting CD3ε and Her2, designed to create varying intermembrane distances (A < B < C < D).

Advancements in biomaterials and scaffold design for tendon repair and regeneration.

Tendon injuries present a significant clinical challenge due to their limited natural healing capacity and the mechanical demands placed on these tissues. This review provides a comprehensive evaluation of the current strategies and advancements in tendon repair and regeneration, focusing on biomaterial innovations and scaffold design. Through a systematic literature search of databases such as PubMed, Scopus, and Web of Science, key studies were analyzed to assess the efficacy of biocompatible materials like hydrogels, synthetic polymers, and fiber-reinforced scaffolds in promoting tendon healing.

The role of LECT2 in kidney fibrosis progression and endoplasmic reticulum stress.

Aims: Our previous studies showed that Leukocyte Cell-Derived Chemotaxin 2 (LECT2), as a ligand for Tie1, modulates vascular endothelial cell function, promoting hepatic fibrosis. These findings prompted an investigation into whether LECT2 effects kidney fibrosis. This study aimed to elucidate the role of LECT2 in kidney fibrosis and its regulation of C/EBP homologous protein (CHOP) expression.

Longitudinal Associations Between Baseline Sarcopenia and Knee Osteoarthritis Progression and Risk of Knee Replacement.

Objective: Sarcopenia and knee osteoarthritis (KOA) are common conditions in older adults, but their relationship is controversial. We aimed to examine the potential role of sarcopenia in KOA progression and subsequent knee replacement (KR).

Methods: Using data from the Osteoarthritis Initiative, baseline sarcopenia was first screened according to the EWGSOP2 algorithm using the SARC-F (Strength, Assistance with walking, Rise from a chair, Climb stairs, and Falls) questionnaire (screened sarcopenia [Scre-S]), then further assessed combined with the five times chair-stand-test (probable sarcopenia [Prob-S]).

The Potential and Challenges of Human Pluripotent Stem Cells in the Treatment of Diabetic Nephropathy.

Diabetic nephropathy (DN) is a prevalent complication of diabetes, with current treatment options offering limited effectiveness, particularly in advanced stages. Human pluripotent stem cells (hPSCs), particularly induced PSCs (iPSCs), show promising potential in the treatment of DN due to their pluripotency, capacity for differentiation into kidney-specific cells, and suitability for personalized therapies. iPSC-based personalized approaches can effectively mitigate immune rejection, a common challenge with allogeneic transplants, thus enhancing therapeutic outcomes.

Advanced Diagnosis and Treatment Methods of High-Pressure Injection Injury: A Single-Center Retrospective Case Series Study.

Aim: This article emphasizes the essential role of radiological imaging in high-pressure injection injury (HPII) management and assesses the results of Magnetic Resonance Imaging (MRI) examinations in providing better details about foreign material extension and assisting surgeons in visualizing operational movement during surgery. Additionally, it shares the authors' experience in managing 16 HPII patients and investigates the application of bone cement in the treatment of injection injuries.

Methods: Data collection of HPII patients was performed between January 2020 and June 2022 in our department, with 16 HPII patients hospitalized with mild to severe high injection injuries.

Case Report: Rheumatoid arthritis patient with hip joint infection and acetabular protrusion undergoing total hip arthroplasty: a case study and literature review.

The chronic autoimmune disease rheumatoid arthritis (RA) affects primarily the synovial joints, leading to hip joint deformity and dysfunction manifestations such as acetabular protrusion and joint infection. We present the case of a female patient RA complicated by severe hip arthritis and acetabular protrusion syndrome. The disease progressed rapidly with elevated preoperative inflammatory markers, initially overlooking hip joint infection.

FEED8 Biosynthesis of Pyranoanthocyanin (Cyanidin-3-glucoside-4-vinylcatechol) Improves Oxidative Stress and Inflammation of the Gut Microbiome in Cadmium-Exposed Mice.

The study is to explore the biosynthesis of cyanidin-3-glucoside-4-vinylcatechol (C3G_VC) through -fermented caffeic acid and cyanidin-3-glucoside (C3G) extract (molar ratio = 1:30) in the model medium. C3G_VC was isolated and purified by a venusil ASB-C18 column with a medium-pressure liquid chromatography (MPLC) system. The chemical structure of C3G_VC was identified by high-performance liquid chromatography (HPLC), which showed the maximum absorption wavelength of 505.

Accelerating the conversion of black chokeberry anthocyanins toward vinylphenolic pyranoanthocyanins by displaying phenolic acid decarboxylase from Lactiplantibacillus plantarum on the surface of Pichia pastoris.

In fermented chokeberry products, hydroxycinnamic acids are enzymatically converted into 4-vinyl derivatives by phenolic acid decarboxylase (PAD), which react with anthocyanins (ACNs) to form stable pyranoanthocyanins (PACNs) that enhance color stability and exhibit excellent bioactivity. However, the fermentation process is usually acidic, the level of PAD secreted by microorganisms is limited and PAD has poor acid stability, resulting in low PACN production. To overcome this, we engineered a whole-cell biocatalyst (WCB) by displaying PAD from Lactiplantibacillus plantarum on Pichia pastoris GS115 (dLPPAD).

Leukocyte cell-derived chemotaxin 2 (LECT2) regulates liver ischemia-reperfusion injury.

Background And Aim: Hepatic ischemia-reperfusion injury (IRI) is a significant challenge in liver transplantation, trauma, hypovolemic shock, and hepatectomy, with limited effective interventions available. This study aimed to investigate the role of leukocyte cell-derived chemotaxin 2 (LECT2) in hepatic IRI and assess the therapeutic potential of Lect2-short hairpin RNA (shRNA) delivered through adeno-associated virus (AAV) vectors.

Materials And Methods: This study analyzed human liver and serum samples from five patients undergoing the Pringle maneuver.

One-Step Formed Janus Hydrogel with Time-Space Regulating Properties for Suture-Free and High-Quality Tendon Healing.

Janus hydrogels have promising applications in tendon healing and anti-peritendinous adhesions. However, their complicated preparation methods, weak mechanical properties, and unstable adhesion interfaces have severely limited their application in suture-free and high-quality tendon healing. In this work, by controlling the interfacial distribution of free -COOH groups and cationic-π structures on both sides of the hydrogels, a series of PZBA-EGCG-ALC Janus hydrogels with varying degrees of asymmetric properties are successfully prepared using a simple and efficient one-step synthesis method.

External Validation of the Simple Postoperative Acute Kidney Injury Risk Index in Patients Admitted to the Intensive Care Unit After Noncardiac Surgery.

Background: The Simple Postoperative AKI Risk (SPARK) index is a novel model for predicting risk of postoperative acute kidney injury (PO-AKI) among patients after noncardiac surgery. However, the performance of the index has been inconsistent partly due to heterogeneity in case mix and effects of the involved clinical features. To clarify potential reasons for poor performance, we tested the SPARK index in a cohort of high-risk patients requiring intensive care unit (ICU) care after noncardiac surgery and examined whether model modification by refitting coefficients of clinical features could optimize model performance.

Zinc pretreatment for protection against intestinal ischemia-reperfusion injury.

Background: Intestinal ischemiareperfusion (I/R) injury (II/RI) is a critical condition that results in oxidative stress, inflammation, and damage to multiple organs. Zinc, an essential trace element, offers protective benefits in several tissues during I/R injury, but its effects on intestinal II/RI remain unclear.

Aim: To investigate the effects of zinc pretreatment on II/RI and associated multiorgan damage.

The Role of Fibrinogen and Platelets in Mouse Liver Ischemia-Reperfusion Injury: Distribution and Pathophysiological Insights.

Liver ischemia-reperfusion (I/R) injury is a critical issue in clinical settings, particularly in liver transplantation and resection, leading to severe hepatocellular dysfunction and organ failure. This study investigates the role of fibrinogen and platelets in liver I/R injury, focusing on their distribution and pathophysiological impact within liver lobules. Using a mouse model, we examined the expression and localization of fibrinogen and platelets at various time points postreperfusion.

IRF1 transcriptionally up-regulates CXCL10 which increases CD8 T cells infiltration in colorectal cancer.

Tumor-infiltrating CD8 T cell is a robust predictor of outcome and immunotherapy response in patients with CRC. However, limited introduction of intratumoral CD8 T cells remains a barrier for treatment of CRC. One of the most effective but difficult therapy for CD8 T cells entering the tumor is activating chemokine receptors.

A1 Pulley Reconstruction for Severe Trigger Finger.

The aim of this study was to evaluate the effectiveness of A1 pulley reconstruction in treating severe trigger fingers and to compare its outcomes with those of the traditional A1 pulley release technique. A total of 43 patients participated in the study, divided into two groups: 22 patients underwent A1 pulley reconstruction, while the remaining 21 patients received the standard A1 pulley release procedure. The outcomes were assessed using the Michigan Hand Outcomes Questionnaire (MHQ) 1 month post-surgery.

siRNAs Targeting Non-Human Species-Specific lncRNAs Trigger Cell Death in Human Colorectal Cancer Cells.

Species-specific long non-coding RNAs (lncRNAs) possess numerous unknown functions. We have recently reported that short interfering RNAs (siRNAs) designed to target mouse-specific lncRNAs caused cell death exclusively in human cancer cells, sparing normal human cells and mouse cancer cells. However, it is uncertain whether other non-human species-specific lncRNAs could also be applied as sequential targets for designing anti-tumor therapeutic siRNAs.