Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis.

This study explores the effects of selenium-enriched yeast supplementation on growth-related and immune-related gene expression in , aiming to support feed optimization in oyster aquaculture. Selenium, an essential trace element, is vital for growth, immune function, and metabolism in animals. Selenium-enriched yeast, an organic form, offers superior bioavailability, enabling efficient absorption and utilization.

A Predictive Nomogram Model Incorporating Contrast Extravasation for Early Prediction of Postthrombectomy Malignant Cerebral Edema.

Objective: Early prediction of malignant cerebral edema (MCE) following endovascular thrombectomy (EVT) is critical for guiding timely interventions to improve outcomes; however, existing prediction models predominantly require complex algorithms. Contrast extravasation (CE) is a novel imaging biomarker. We aimed to develop a practical nomogram incorporating CE for predicting MCE to enhance postoperative management.

The galectin family in large yellow croaker (Larimichthys crocea): Genome-wide identification, stress-responsive regulation to Pseudomonas plecoglossicida infection and hypoxia, and functional validation of LcGal-2a.

Galectins are carbohydrate-binding proteins involved in many physiological functions, including immune response and cell migration. Although galectins have been studied in various fish species, their functional roles and expression patterns in large yellow croaker (Larimichthys crocea) remain poorly understood. In this study, 13 galectin genes (Lcgalectins) were identified using the whole genome database of large yellow croaker.

Oxidative Stress, Energy Metabolism Disorder, Mitochondrial Damage, and miR-144 Participated in Molecular Mechanisms of 4-Octylphenol-Caused Cardiac Autophagic Damage in Common Carps ( L.).

: In 4-octylphenol (4-OP), a toxic environmental pollutant with endocrine disruptive effect, the use of 4-OP causes pollution in the freshwater environment and poses risks to aquatic organisms. Common carps ( L.) live in freshwater and are experimental animals for studying the toxic effects of environmental pollutants on fish.

Efficacy and Safety of Ivarmacitinib, a Selective JAK1 Inhibitor, in Patients With Active Ankylosing Spondylitis: Results From an Adaptive Seamless Phase 2/3 Trial.

Objective: This randomized, double-blind, adaptive phase 2/3 trial aimed to evaluate the efficacy and safety of ivarmacitinib, a novel selective JAK1 inhibitor, in patients with active ankylosing spondylitis (AS).

Methods: In phase 2, patients were randomized (1:1:1:1) to receive ivarmacitinib at a dose of 2, 4, or 8 mg or placebo once daily for 12 weeks. Based on interim analysis, ivarmacitinib 4 mg was selected as the recommended dose.

Bleeding prediction scores in patients with venous thromboembolism using direct oral anticoagulants.

We aimed to identify factors associated with bleeding in patients with venous thromboembolism (VTE) when using direct oral anticoagulants (DOACs), to construct and externally validate a predictive model for bleeding, and to provide a validated tool for clinical assessment of bleeding. The prediction model in the development cohort was constructed using logistic regression and visualized through a Nomogram. We conducted external validation of the model.

Molecular cloning, expression, and functional analysis of cystatin B from silver pomfret (Pampus argenteus) under Vibrio parahaemolyticus challenge.

Cystatin B is an intracellular inhibitor that regulates the activity of cysteine proteases. In this study, the coding sequence of silver pomfret cystatin B was successfully cloned and identified, which was 297 bp in length, predicted to encode 98 amino acids with a molecular weight of approximately 11.12 kDa.

ROS acted as an initial role in selenium nanoparticles alleviating insecticide chlorpyrifos-induced oxidative stress, pyroptosis, and intestinal barrier dysfunction in porcine intestinal epithelial cells.

Chlorpyrifos (CPF), a toxic organophosphorus insecticide, is widely used in agriculture to protect crops (eg., maize) from pests. The use of CPF in crops can result in accumulation in crop seeds, such as corn seeds, which is a primary feed ingredient in pigs.

The toxicological effects of perfluorooctanoic acid (PFOA) exposure in large yellow croaker (Larimichthys crocea): exploring the relationship between liver damage and gut microbiota dysbiosis.

Per- and polyfluoroalkyl substances (PFASs) are synthetic organofluorine compounds characterized by their persistence, toxicity, and bioaccumulative properties, rendering them substantial environmental contaminants. However, limited research has investigated the effects of a short-term low-concentration PFAS exposure on the hepatic and intestinal systems of marine fish. In this study, large yellow croaker was selected as the experimental subject to explore the toxic effects of exposure to 1000 ng/L PFOA after 3, 7, and 14 days, with a focus on liver and gut microbiota.

Hybrid NIR-responsive liposome/hydrogel platform mediating chemo-photothermal therapy of retinoblastoma enhanced by quercetin as an adjuvant.

Retinoblastoma is one of the most aggressive paediatric cancers originating from retina and finally invades vitreous humour or other tissues if treated improperly. Current interventions often fail due to the complexities of tumour progression and drug resistance driven by frequent intravitreal injection and epithelial-mesenchymal transition (EMT). Consequently, there is an unmet need for more effective, less invasive treatments.

The mechanism of lycopene alleviating cadmium-inhibited glucose uptake ability of epithelioma papulosum cyprini cells: miR-375, oxidative stress, and actin cytoskeleton dysfunction.

Cadmium (Cd) poses a threat to fish and human health. Carp is the most widely farmed fish, and it is necessary to study the mechanism of Cd toxicity and effective mitigation methods for Cd poisoning in carps. We previously found that Cd up-regulated miR-375 in common carp spleens, and that IRS1, a factor involved in glucose (GLU) uptake, was a potential target gene of miR-375.

Comparative transcriptome analysis reveals differences in immune responses to copper ions in Sepia esculenta under high-temperature conditions.

Sepia esculenta is one of the most abundant extant squid populations in Southeast Asia and is of interest due to its rapid reproductive rate and high commercial value. In recent years, with the rapid development of industrialization, issues such as global warming and heavy metal pollution in the oceans have emerged, posing a serious threat to the life activities of marine organisms. In this study, we used transcriptomic techniques to investigate the differences in Cu exposure immune responses in S.

Co-exposure to ammonia and lipopolysaccharide-induced impaired energy metabolism via the miR-1599/HK2 axis and triggered autophagy, ER stress, and apoptosis in chicken cardiomyocytes.

Ammonia (NH) and lipopolysaccharide (LPS), common pollutants in poultry farming environments, pose significant health risks by disrupting cellular processes. Although previous studies have demonstrated the individual effect of NH or LPS on human and animal health, the mechanisms underlying their combined impact on chicken heart tissue remain poorly understood. In this study, we established a chicken cardiotoxicity model to investigate the effects of NH and/or LPS exposure on energy metabolism, autophagy, endoplasmic reticulum (ER) stress, and apoptosis in cardiomyocytes.

Amino acid metabolism disorder and oxidative stress took part in EGCG alleviating Mn-caused ferroptosis via miR-9-5p/got1 axis.

Manganese (Mn) is not only an essential trace metal element in organisms, but also a water pollutant and damaging aquatic organisms. (-)-epigallocatechin gallate (EGCG), a natural tea polyphenol derived from green tea, may alleviate toxicity caused by environmental pollutants. Kidney is an important organ for excretion and expel toxins.

Development and validation of the Parent-Reported Indicator of Developmental Evaluation for Chinese Children (PRIDE) tool.

Background: Developmental delay (DD) poses challenges to children's overall development, necessitating early detection and intervention. Existing screening tools in China focus mainly on children with developmental issues in two or more domains, diagnosed as global developmental delay (GDD). However, the recent rise of early childhood development (ECD) concepts has expanded the focus to include not only those with severe brain development impairments but also children who lag in specific domains due to various social-environmental factors, with the aim of promoting positive development through active intervention.

Construction of nitroreductase-responsive near-infrared composite nanoprobe and its application in tumor hypoxia imaging.

Hypoxia is an important feature in the development of solid tumors. Nitroreductase (NTR) is closely related to the level of hypoxia, thus NTR-responsive fluorescent probes with high selectivity and sensitivity will help to evaluate the degree of hypoxia and guide personalized treatment. In this study, a near-infrared (NIR) NTR-activated composite nanoprobe 780-pNBC@MP-B was developed for tumor hypoxia imaging.

No difference in outcomes with early vs late antibiotic prophylaxis for term premature rupture of membranes: a multicenter analysis.

Background: Although guidelines suggest administering antibiotics 12 to 18 hours after the rupture of membranes in women with term premature rupture of membranes, in practice, clinicians tend to initiate prophylactic antibiotics as soon as possible to avoid risk of infection.

Objective: This study aimed to assess whether early administration of prophylactic antibiotics for term premature rupture of membranes reduces the incidence of maternal and neonatal infections.

Study Design: This multicenter prospective cohort study included women with term premature rupture of membranes.

Effects of allergic diseases on social-emotional development in children at 12 months of age: A Prospective Cohort Study.

Objectives: The link between allergic diseases and deficits in children's neurodevelopment has been suggested, but it remains unclear regarding the allergy-related effects on social-emotional development in early life. Our study aimed to explore the association between allergic diseases and social-emotional development during infancy using a prospective study.

Methods: 937 infants at 6 months were recruited from two community hospitals in Shanghai, of which 805 infants followed up at 12 months.

Transcriptome profiling of triploid Crassostrea gigas gills indicates the host immune mechanism against bacterial infection.

As an important member of global aquaculture, oysters (Crassostrea gigas) have significant economic value. With the development of commercial aquaculture, the frequent occurrence of diseases caused by Vibrio alginolyticus has become a hindrance to high-density aquaculture. Gill tissue, as an important component of immune system of the oysters, plays the key point in the face of invasion by foreign substances.

Risk of Bleeding, Thrombosis and Death among Atrial Fibrillation Patients Treated with Oral Anticoagulants Across Estimated Glomerular Filtration Rates.

There are limited data about the clinical benefits and harm of oral anticoagulants (OACs) for stroke prevention in patients with atrial fibrillation (AF) and chronic kidney disease using CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) creatinine equation for glomerular filtration rate (GFR) estimation in nuanced GFR stratification. We conducted a retrospective study in 12 centers in China and included 9,510 patients with AF. We grouped patients into the following estimated GFR (eGFR) categories: ≥60 (n = 7,616), 45 to 59 (n = 1,139), 30 to 44 (n = 474), and <30 (n = 281) ml/min/1.

Transcriptome analysis reveals the immune response mechanism of golden cuttlefish (Sepia esculenta) larvae exposed to ink.

The golden cuttlefish (Sepia esculenta), a significant cephalopod in the Yellow and Bohai Seas of China, is highly esteemed for its exceptional medicinal and commercial value. The natural resources of the S. esculenta are currently facing depletion due to the ongoing environmental degradation and overfishing.

Identification, molecular profiling and immune functions of cystatin M in silver pomfret (Pampus argenteus).

Cystatins play an important role in various physiological and pathological processes of organisms, including regulating protein metabolism, antigen processing, inflammatory response, nutritional disorders, and controlling enzyme activity. However, research on immunity functions of fish cystatin M is limited. In this study, Pampus argenteus cystatin M (Pacystatin M) was identified and analyzed.

[Liposome co-delivery of quercetin and doxorubicin in inhibiting retinoblastoma by regulating epithelial-mesenchymal transition process].

Regulating the process of epithelial-mesenchymal transition(EMT) is an essential strategy to inhibit tumor growth and metastasis. This study is based on the EMT process of retinoblastoma and constructs quercetin(QUE) and doxorubicin(DOX) co-loaded liposome(QD Lipo) to investigate the therapeutic effect and mechanisms of combined QUE and DOX treatment on retinoblastoma. Single-factor experiments were conducted to optimize the prescription process of QD Lipo.

Identification of COMMD gene family in large yellow croaker (Larimichthys crocea): Immune response induced by Pseudomonas plecoglossicida infection and acute hypoxia stress.

The COMMD (Copper Metabolism gene MURR1 Domain) gene family consists of 10 members, which are involved in various biological processes such as copper and sodium transport, NF-κB activity and cell cycle progression. However, the study of COMMD gene family in large yellow croaker (Larimichthys crocea) is largely unknown. In this study, 10 COMMD gene family members (named LcCOMMDs) were successfully identified from large yellow croaker.