Localized Hydrolysis-Based Proximity Reporting Platform for Protein Hierarchical Structure Profiling.

The substrate-specific bond cleavage capability of hydrolases provides a unique strategy for engineering off-on switches. This prompted our hypothesis that confining hydrolases and substrates to distinct biomolecular hierarchies (e.g.

JNK/c-Jun cascade activation enhances malignant proliferation of psoriatic keratinocytes by regulating ROS levels and mitochondrial membrane potential.

Psoriasis is a chronic immune-mediated skin disorder characterized by excessive keratinocyte proliferation, inflammation, and oxidative stress. This study investigates the role of the JNK/c-Jun cascade in psoriasis pathogenesis, focusing on its impact on keratinocyte proliferation and inflammatory responses. An in vitro psoriasis model was established using M5-stimulated HaCaT keratinocytes, while an in vivo model was created with imiquimod-treated Wistar rats.

Molecular cloning, expression, and functional analysis of cystatin B from silver pomfret (Pampus argenteus) under Vibrio parahaemolyticus challenge.

Cystatin B is an intracellular inhibitor that regulates the activity of cysteine proteases. In this study, the coding sequence of silver pomfret cystatin B was successfully cloned and identified, which was 297 bp in length, predicted to encode 98 amino acids with a molecular weight of approximately 11.12 kDa.

The toxicological effects of perfluorooctanoic acid (PFOA) exposure in large yellow croaker (Larimichthys crocea): exploring the relationship between liver damage and gut microbiota dysbiosis.

Per- and polyfluoroalkyl substances (PFASs) are synthetic organofluorine compounds characterized by their persistence, toxicity, and bioaccumulative properties, rendering them substantial environmental contaminants. However, limited research has investigated the effects of a short-term low-concentration PFAS exposure on the hepatic and intestinal systems of marine fish. In this study, large yellow croaker was selected as the experimental subject to explore the toxic effects of exposure to 1000 ng/L PFOA after 3, 7, and 14 days, with a focus on liver and gut microbiota.

Glycan Covalent Targeting Chimera-Based T-Cell Glycoengineering for Boosting Immunotherapy.

Chimeric antigen receptor (CAR) T-cell therapy is one of the most effective approaches in cancer immunotherapy. However, shortcomings such as loss of target antigens and poor infiltration remain in the treatment of solid tumors. Herein, we propose an approach to glycoengineer T cells based on glycan covalent targeting chimera (gcTAC), to enhance immunotherapy by modulating the glycan recognition behavior of T cells.

Climate Change and Human Pressure: Assessing the Vulnerability of Snow Leopard () Habitat Integrated With Prey Distribution on the Qinghai-Tibet Plateau.

Climate change is significantly altering the distribution of large carnivores and their primary prey species, with particular emphasis on the changing prey distribution in high-altitude regions. The Qinghai-Tibet Plateau, known for its rich biodiversity, is highly sensitive to climate change, affecting the habitats of snow leopards () and blue sheep (). Our study identified blue sheep as the primary prey of snow leopards through metagenomic analysis and used bioclimatic data and Land Use/Cover Change (LUCC) information to model habitat suitability under three climate scenarios (RCP 2.

METTL14-mediated mA methylation of pri-miR-5099 to facilitate cardiomyocyte pyroptosis in myocardial infarction.

N-methyladenosine (mA) modification is an important mechanism in microRNA processing and maturation. Previous studies show the involvement of pri-miRNA methylation in regulating the occurrence and development of tumor-related diseases. In this study, we investigated the role of its aberrant regulation in cardiac diseases.

A split-type photoelectrochemical sensor based on InS/PCN-224 Z-scheme heterojunction for ultrasensitive detection of ampicillin.

A ultrasensitive split-type photoelectrochemical (PEC) sensor was constructed for ampicillin (AMP) detection, utilizing a metal-organic framework (MOF)-confined InS/PCN-224 Z-scheme heterojunction as the photoactive material. The prepared InS/PCN-224 was demonstrated with high charge separation efficiency and a stable PEC signal response due to the unique electron flow direction of the Z-scheme configuration. To further enhance the detection sensitivity, target-mediated in-situ ion exchange via Cd ions was employed to modulate the photoactivity of InS/PCN-224.

Target-regulated AgS/FeOOH heterojunction activity: a direct label-free photoelectrochemical immunosensor.

Myoglobin (Mb), an important cardiac marker, plays a crucial role in diagnosing, monitoring, and evaluating the condition of patients with cardiovascular diseases. Here, we propose a label-free photoelectrochemical (PEC) sensor for the detection of Mb through target regulated the photoactivity of AgS/FeOOH heterojunction. The AgS/FeOOH nanospindles were synthesized and served as a sensing platform for the fabrication of bio-recognized process for Mb.

Long-term Outcomes of Prostate Capsule-Sparing and Nerve-Sparing Radical Cystectomy With Neobladder: A Propensity Score-Matched Comparison.

Purpose: This study aimed to compare and analyze the feasibility and long-term efficacy of prostatic capsule-sparing (PCS) and nerve-sparing (NS) radical cystectomy in the treatment of bladder cancer.

Methods: From June 2004 to December 2021, our institution treated and followed 145 patients who underwent radical cystectomy with neobladder reconstruction for over a year. These patients were divided into 2 groups: PCS (n=74) and NS (n=71).

Target-assisted self-powered photoelectrochemical sensor based on AgS/BiOCl heterojunction for ultrasensitive chlorpyrifos detection.

Chlorpyrifos (CPF), a widely used organophosphorus pesticide, presents substantial risks to both environmental and human health due to its persistent accumulation, thereby necessitating the development of effective detection methods. Self-powered photoelectrochemical (PEC) sensors, as an innovative technology, address the limitations inherent in conventional sensors, such as susceptibility to interference and inadequate signal response. Herein, we synthesized AgS/BiOCl as a photosensitive material, employing it as a light-harvesting substrate and a signal-transducing platform to develop a self-powered PEC sensor for the detection of CPF.

Brg1 and RUNX1 synergy in regulating TRPM4 channel in mouse cardiomyocytes.

Background: Transient Receptor Potential Melastatin 4 (TRPM4), a non-selective cation channel, plays a critical role in cardiac conduction abnormalities. Brg1, an ATP-dependent chromatin remodeler, is essential for regulating gene expression in both heart development and disease. Our previous studies demonstrated Brg1 impacted on cardiac sodium/potassium channels and electrophysiological stability, its influence on TRPM4 expression and function remained unexplored.

Importance of biopsy sample length for cancer diagnosis during trans-perineal prostate biopsy.

Objective: To identify the factors that determine the minimum length of biopsy sample required for accurate diagnosis.

Materials And Methods: A retrospective analysis was conducted on 1202 cases that underwent rectal ultrasound-guided trans-perineal prostate biopsy (TPB) with standardized biopsy surgical procedures and pathological evaluation. Logistic regression correlation analysis and the imbalance between groups was eliminated by propensity score matching of patients' own factors between groups (positive group and negative group).

Photoreceptor-targeted extracellular vesicles-mediated delivery of Cul7 siRNA for retinal degeneration therapy.

Photoreceptor loss is a primary pathological feature of retinal degeneration (RD) with limited treatment strategies. RNA interference (RNAi) has emerged as a promising method of gene therapy in regenerative medicine. However, the transfer of RNAi therapeutics to photoreceptors and the deficiency of effective therapeutic targets are still major challenges in the treatment of RD.

Identification, molecular profiling and immune functions of cystatin M in silver pomfret (Pampus argenteus).

Cystatins play an important role in various physiological and pathological processes of organisms, including regulating protein metabolism, antigen processing, inflammatory response, nutritional disorders, and controlling enzyme activity. However, research on immunity functions of fish cystatin M is limited. In this study, Pampus argenteus cystatin M (Pacystatin M) was identified and analyzed.

Identification of COMMD gene family in large yellow croaker (Larimichthys crocea): Immune response induced by Pseudomonas plecoglossicida infection and acute hypoxia stress.

The COMMD (Copper Metabolism gene MURR1 Domain) gene family consists of 10 members, which are involved in various biological processes such as copper and sodium transport, NF-κB activity and cell cycle progression. However, the study of COMMD gene family in large yellow croaker (Larimichthys crocea) is largely unknown. In this study, 10 COMMD gene family members (named LcCOMMDs) were successfully identified from large yellow croaker.

Self-shedding MOF-nanocarriers modulated CdS/MoSe heterojunction activity through in-situ ion exchange: An enhanced split-type photoelectrochemical sensor for deoxynivalenol.

Deoxynivalenol (DON), a mycotoxin produced by Fusarium, poses a significant risk to human health and the environment. Therefore, the development of a highly sensitive and accurate detection method is essential to monitor the pollution situation. In response to this imperative, we have devised an advanced split-type photoelectrochemical (PEC) sensor for DON analysis, which leverages self-shedding MOF-nanocarriers to modulate the photoelectric response ability of PEC substrate.

Long-term follow-up results of prostate capsule-sparing and nerve-sparing radical cystectomy with neobladder: a single-center retrospective analysis.

Objective: This study aims to investigate and analyze the feasibility, oncological outcomes, functional efficacy, and complications with the prostatic capsule sparing (PCS) as well as the nerve sparing (NS) in radical cystectomy for bladder cancer.

Patients And Methods: Between January 2007 and December 2021, 67 total cystectomies with PCS and 54 with NS were performed at our institution. The inclusion criteria for PCS were as follows: proactive, fully informed patient consent; negative transurethral resection of the bladder neck; normal prostate-specific antigen (PSA) level < 4 ng/dL; and normal transrectal ultrasonography with biopsy of any suspicious nodes.

miR-133a-3p/TRPM4 axis improves palmitic acid induced vascular endothelial injury.

Vascular endothelial injury is a contributing factor to the development of atherosclerosis and the resulting cardiovascular diseases. One particular factor involved in endothelial cell apoptosis and atherosclerosis is palmitic acid (PA), which is a long-chain saturated fatty acid. In addition, transient receptor potential melastatin 4 (TRPM4), a non-selective cation channel, plays a significant role in endothelial dysfunction caused by various factors related to cardiovascular diseases.

A genome-wide association study identifies genes associated with cuticular wax metabolism in maize.

The plant cuticle is essential in plant defense against biotic and abiotic stresses. To systematically elucidate the genetic architecture of maize (Zea mays L.) cuticular wax metabolism, 2 cuticular wax-related traits, the chlorophyll extraction rate (CER) and water loss rate (WLR) of 389 maize inbred lines, were investigated and a genome-wide association study (GWAS) was performed using 1.

Identification of large yellow croakers (Larimichthys crocea) scavenger receptor genes: Involvement in immune response to Pseudomonas plecoglossicida infection and hypoxia-exposure experiments.

Scavenger receptors (SRs) are pattern recognition receptors involved in the innate immune defense against pathogen infection in fish. However, there has not been much research done on teleosts. In this study, 18 members of the SR gene family were found in large yellow croaker.

A P-type pentatricopeptide repeat protein ZmRF5 promotes 5' region partial cleavages of atp6c transcripts to restore the fertility of CMS-C maize by recruiting a splicing factor.

A fast evolution within mitochondria genome(s) often generates discords between nuclear and mitochondria, which is manifested as cytoplasmic male sterility (CMS) and fertility restoration (Rf) system. The maize CMS-C trait is regulated by the chimeric mitochondrial gene, atp6c, and can be recovered by the restorer gene ZmRf5. Through positional cloning in this study, we identified the nuclear restorer gene, ZmRf5, which encodes a P-type pentatricopeptide repeat (PPR) family protein.