Computational Analyses Identified Three Diagnostic Biomarkers Associated With Programmed Cell Death for Lung Adenocarcinoma.

The high morbidity and mortality of lung adenocarcinoma (LUAD) are partly caused by a lack of sensitive and reliable molecular markers for early diagnosis. Programmed cell death (PCD) is a crucial process involved in tumorigenesis and immune regulation, and identifying PCD-correlated genes may contribute to the precision diagnosis and targeted therapy of LUAD. LUAD samples were acquired from UCSC Xena and Gene Expression Omnibus (GEO) database.

Signal Propagation in Surface-Confined DNA Circuits with Rigidified DNA Origami.

Surface-confined DNA computing has emerged as a powerful information processing paradigm, offering enhanced specificity and accelerated reaction kinetics. Artificially designed DNA origami serves as a key enabler for such systems by providing a highly programmable platform for positioning computational components with nanometer resolution. However, conventional monolayer DNA origami circuits often exhibit non-negligible signal leakage, attributed to structural fluctuation-induced crosstalk between surface-confined molecules.

Designing a DNA synchronizer for compact single-rail DNA logic circuits.

DNA has emerged as a robust platform for engineering molecular circuits with arbitrary logic operations. Nevertheless, implementing DNA circuits for such functions generally relies on the use of dual-rail expression that doubles the number of required gates, constraining the achievable complexity in a single solution. A fundamental limitation is that conventional single-rail circuits cannot support nonfirst-layer NOT operations.

Disturbance Observer-Based Adaptive Synchronization of Heterogeneous Chaotic Systems With Uncertainties via DNA Strand Displacement.

The application of DNA strand displacement (DSD) for synchronizing chaotic systems has made remarkable progress in recent years. However, uncertainty may exist in the systems, and interference is unavoidable during the synchronization process. This article endeavors to tackle the issue of performance degradation in synchronization caused by disturbances and system uncertainties.

Ni-Catalyzed Asymmetric Reductive Addition of Aromatic Iodides to Isatins: A General Access to Chiral 3-Hydroxy-2-Oxindoles.

A nickel-catalyzed enantioselective reductive addition of aryl iodides with isatins has been developed, enabled by a newly designed FOXAP-type ligand bearing a distal bulky silyl group. The reaction proceeds under mild conditions with Mn as the reductant, delivering 3-aryl-3-hydroxyoxindoles in high yields and good to excellent enantioselectivities. This protocol exhibits a broad substrate scope and provides a practical and efficient approach for the synthesis of valuable chiral 3-hydroxy-2-oxindoles bearing a quaternary stereocenter.

Comparative differences in job preferences among doctors in different levels of public hospitals in Henan, China: a discrete choice experiment.

Background: Doctor turnover is a significant challenge faced by hospitals worldwide, particularly in public hospitals in China. By understanding how job attributes influence job preferences among doctors, effective and targeted policies can be designed to attract doctors to key positions or organizations, providing insights for evidence-based workforce interventions.

Objectives: The aim of our study is to examine the difference of job preferences of doctors in different hospitals levels using DCE.

Locked Nucleic Acid Modification for Base-Stacking Engineering of Self-Assembled DNA Crystals.

DNA hybridization and assembly processes are governed by the concerted effects of Watson-Crick pairing and base-stacking interactions. While sequence engineering and chemical modifications have been extensively exploited to regulate DNA hybridization processes and complex structural assembly of DNA, here we demonstrate the use of locked nucleic acid (LNA) modifications to finely tune base-stacking interactions, which are yet to be explored in DNA assembly processes, to program the growth of self-assembled DNA crystals. We find that sticky-end LNA modifications decrease base-pair spacing and enhance base-stacking energy, which synergistically improves interstrand affinity and accelerates hybridization rate constants, as revealed by strand displacement kinetics, molecular dynamics simulations, and small-angle X-ray scattering analysis.

Population-based colorectal cancer risk prediction using a SHAP-enhanced LightGBM model.

Background: Colorectal cancer (CRC) is a highly frequent cancer worldwide, and early detection and risk stratification playing a critical role in reducing both incidence and mortality. we aimed to develop and validate a machine learning (ML) model using clinical data to improve CRC identification and prognostic evaluation.

Methods: We analyzed multicenter datasets comprising 676 CRC patients and 410 controls from Guigang City People's Hospital (2020-2024) for model training/internal validation, with 463 patients from Laibin City People's Hospital for external validation.

Antibacterial Activity of Non-Biaryl-Linked Dimeric Tetraxanthones: Insights into Stability and Efficacy against Gram-Positive Bacteria.

Aflaxanthone B (), a non-biaryl-linked dimeric tetraxanthone, was purified from the endophytic fungus sp. CPCC 401464. Four undescribed transformed products, aflaxanthones C-F (), were obtained by treating compound with dimethyl sulfoxide (DMSO).

A global prevalence of turnover intention among doctors: a systematic review and meta-analysis.

Turnover intention (TI) among doctors is a critical issue in healthcare systems worldwide, as it can lead to workforce shortages, increased workload for remaining staff, and negatively impact patient care. This review aimed to explore the prevalence and determinants of TI among doctors, providing a comprehensive understanding of the factors influencing their turnover intentions. A systematic review was conducted using four electronic databases: PubMed, Web of Science, Medline, and Scopus.

Aesculi semen (Aesculus chinensis Bunge and Aesculus wilsonii Rehd): A review of its phytochemistry, pharmacology, clinical application and toxicity.

This article reviews recent advances of Aesculi Semen (AS), focusing on its phytochemistry, pharmacology, clinical application, and toxicity. We searched several databases using specific keywords in PubMed, Web of Science, Cochrane Library, Embase, China National Knowledge Infrastructure, Wanfang Data, and China Science and Technology Journal. The search terms encompassed Aesculi Semen, Suo luozi, Aesculus wilsonii Rehd, Chinese Buckeye Seed, etc.

Association between dietary calcium intake and benign prostatic hyperplasia: a population-based result from NHANES 2003 to 2008.

Background: Benign prostatic hyperplasia (BPH), as a cause of various lower urinary tract symptoms (LUTS), can improve patients' quality of life when effectively controlled. To elaborate on the effect of dietary calcium intake on BPH, we investigated the association between dietary calcium intake and the risk of BPH using data from the National Health and Nutrition Examination Survey (NHANES).

Methods: Data from the NHANES conducted from 2003 to 2008 were utilized.

A 'two-missile' nanoplatform for targeting triple-negative breast cancer: prodrug activation and immune enhancement.

Targeted cancer therapy remains a significant challenge due to the complexity of tumor microenvironments and the need for precise drug delivery systems that can overcome these obstacles. To address this challenge, a nanoparticle platform based on prodrug activation and immune enhancement for "two-missile" targeting of TNBC has been developed: Azido-HA@CuO@DNA. This nanoparticle platform utilises azido hyaluronic acid (Azido-HA) as the primary carrier to enhance specificity to tumor tissues and targeting to tumor cells.

Relationship between Serum Neurotransmitters and Cognitive Impairment in Adults with Obstructive Sleep Apnea.

Introduction: The primary aim of this study was to investigate the serum levels of acetylcholine (Ach), norepinephrine (NE), dopamine (DA), and 5-hydroxytryptamine (5-HT) in two groups: those with obstructive sleep apnea (OSA) and cognitive impairment (CI) and those with OSA but no cognitive impairment (NCI).

Methods: A total of 30 adults (CI) and 75 adults (NCI) who completed polysomnography examinations between December 2023 and September 2024 were enrolled in the study. Enzyme-linked immunosorbent assays were used to measure serum levels of Ach, NE, DA, and 5-HT.

All-Solid-State S-Scheme Heterojunction TiO/Cu/MoSiN for Efficient Visible-Light Driven Water Splitting.

In the fields of new energy and environmental protection, the development of highly efficient, low-cost, eco-friendly, and stable photoelectrocatalysts has drawn significant interest. Inspired by the high redox potential of S-scheme heterojunctions and the structural advantage of all-solid-state Z-scheme junctions, a novel all-solid-state S-scheme heterojunction TiO/Cu/MoSiN nanorod (NR) array is designed and prepared using hydrothermal and magnetron sputtering methods. Under the synergistic effect of the built-in electric field, high redox potential, and conductive Cu medium, light absorption is extended to the visible-light region, and the separation and transfer efficiencies of the photogenerated carriers are significantly improved.

Magnetic hierarchically mesoporous UIO-66(Ce/Ti) core-shell nanocomposites for efficient enrichment of phosphopeptides.

Hierarchically mesoporous metal-organic frameworks (HMUIO-66(Ce/Ti)) was designed and fabricated firstly by using a relatively rigid hydroxypropyl methylcellulose (HPMC) coupled with poly(ethylene oxide-b-propylene oxide-b-ethylene oxide, P123) as a soft template. By introducing the synergistic effect of Hofmeister ions and the soft template of HPMC coupled with P123 into the water-rich micelle system, the obtained HMUIO-66(Ce/Ti) possessed irregularly mesoporous architecture with a wide pore size distribution of 8.4 ~ 26.

From imaging techniques to laboratory tests: a multimodal stratification for varicocele-associated decreased semen quality.

Background: Varicocele adversely affects male fertility by impairing semen quality, yet there remains no unified approach that combines advanced imaging and laboratory markers for clinical stratification. To develop a uniform clinical stratification system, we conducted a review of the literature across major databases.

Results: Shear wave elastography thresholds above 5.

Single-Molecule DNA Hybridization on Tetrahedral DNA Framework-Modified Surfaces.

Tetrahedral DNA frameworks (TDFs) have been extensively utilized in biosensing systems. At the macroscopic level, it has been well established that TDFs, when employed as probe scaffolds, significantly enhance the interfacial DNA hybridization efficiency between target single-stranded DNA (ssDNA) and probe ssDNA at the solid-liquid interface. However, a molecular-level understanding of how TDF scaffolds facilitate this interfacial hybridization remains elusive.

Robust Accessibility of Addressable Sites in Defective DNA Origami.

Owing to its unique programmability and addressability, DNA origami-based nanofabrication has been widely utilized in fields such as nanomedicine and nanophotonics. The accessibility of addressable sites on DNA origami structures is crucial for their use as nanofabrication platforms. In this study, we systematically investigated the impact of structural defects on accessibility using a classic six-helix bundle (6HB) DNA origami and two variants with subsets of DNA staple strands deleted, introducing programmable defects in 6HB nanostructures.

Neuroadaptive fixed-time fault-tolerant containment control of high-order MIMO Nonlinear multi-agent systems in affine strict-feedback form.

This paper is concerned with the fixed-time containment control problem for high-order MIMO nonlinear multi-agent systems with external disturbances and actuator faults. First, in the backstepping framework, a neuroadaptive fixed-time containment control protocol is skillfully developed under an undirected communication topology. Second, criteria for designing desired fault-tolerant fixed-time containment control protocol are derived such that the containment performance of the resulting closed-loop MIMO multi-agent systems can be guaranteed within a fixed time horizon and avoided the singularity problem.

Prospective multicenter study identifying prognostic biomarkers and microbial profiles in severe CAP using BALF, blood mNGS, and PBMC transcriptomics.

To identify potential biomarkers associated with the prognosis and severity of severe community-acquired pneumonia (SCAP), we conducted a multi-center prospective study from January 1, 2022, to December 31, 2023, enrolling 14 mild CAP and 75 SCAP patients in Shanghai, China. Patients underwent bronchoalveolar lavage fluid (BALF) and blood metagenomic next-generation sequencing (mNGS) as well as peripheral blood mononuclear cell (PBMC) transcriptomics. Among the 75 SCAP patients, 32 died within 30 days, with older age, a history of allergies, and comorbidities like cerebrovascular disease linked to worse outcomes.

Highly sensitive and multifunctional Fe enhanced PVA/gelatin multi-network hydrogels with wide temperature range environmental stability for wearable sensors.

Hydrogels are one of the ideal materials for preparing new flexible sensor devices. In this work, based on the freeze-thaw cycle and Hoffmeister effect to improve the mechanical properties, a low-temperature resistant multifunctional conductive hydrogel with excellent mechanical properties, high transparency, high adhesion, high conductivity, high sensitivity, excellent sensing ability and self-healing ability was prepared by diffusing Fe and ethylene glycol (EG) in the hydrogel network via immersion method. Moreover, wearable sensors assembled from it could accurately monitor the movement of various parts of the human body, and it could accurately identify tiny strain and pressure sensing.

CAMP-negative strains exhibited complete or partial chromosomal deletions of the CAMP-factor encoding gene .

Unlabelled: Universal antepartum group B streptococcus (GBS) screening and intrapartum antibiotic prophylaxis (IAP) have effectively reduced early-onset GBS infections. However, GBS strains with chromosomal deletions affecting the gene may produce false negatives in both the CAMP test and -based molecular diagnostics, potentially increasing the risk of neonatal infections. Vaginal swabs were collected from pregnant women at 35-37 weeks of gestation in our hospital and cultured on agar.

Uniportal endoscopic decompression and debridement for infectious diseases of spine with neurological deficits: a retrospective study in China.

Study Design: A retrospective study.

Purpose: To evaluate the clinical efficacy of uniportal endoscopic decompression and debridement (UEDD) in treating infectious diseases of the spine (IDS) with neurological deficits.

Overview Of Literature: IDS patients with neurological deficits often require urgent surgical decompression.

Synergistic Effect of Topological Semimetal TiSi and Plasmonic Cu for Enhanced Photoelectrocatalytic Water Splitting of TiO Nanorod Array.

Developing high-efficiency and low-cost catalysts for PEC water splitting is vital for solving the issues of energy consumption, environmental pollution, and global warming. In this study, we designed and prepared a composite photoanode TiO/TiSi/Cu by depositing a topological semimetal TiSi film and plasmonic Cu nanoparticles onto a TiO nanorod array. Due to the synergistic effect of the topological bands of TiSi and the SPR effect of Cu, TiO/TiSi/Cu exhibits significantly enhanced optical absorption in the visible-light region.