Self-assembled ferritin-based nanoparticles elicit a robust broad-spectrum protective immune response against SARS-CoV-2 variants.

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants has resulted in global economic losses and posed a threat to human health. The pandemic highlights the urgent need for an efficient, easily producible, and broad-spectrum vaccine. Here, we present a potentially universal strategy for the rapid and general design of vaccines, focusing on the design and testing of omicron BA.

A virus-like particle candidate vaccine based on CRISPR/Cas9 gene editing technology elicits broad-spectrum protection against SARS-CoV-2.

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with frequent mutations has seriously damaged the effectiveness of the 2019 coronavirus disease (COVID-19) vaccine. There is an urgent need to develop a broad-spectrum vaccine while elucidating the underlying immune mechanisms. Here, we developed a SARS-CoV-2 virus-like particles (VLPs) vaccine based on the Canarypox-virus vector (ALVAC-VLPs) using CRISPR/Cas9.

Vegetation drought risk assessment based on the multi-weight methods in Northwest China.

Vegetation makes an outstanding contribution to the stability of ecosystems and to a certain extent reflects the state of the terrestrial ecosystem. Drought conditions greatly affect the growth and development process of vegetation due to its remarkable stochasticity and complexity. Due to the complex coupling mechanism between vegetation and drought, the research on vegetation drought risk is still limited.

Phylogeny of the plant receptor-like kinase (RLK) gene family and expression analysis of wheat RLK genes in response to biotic and abiotic stresses.

Background: The receptor-like kinase (RLK) gene families in plants contains a large number of members. They are membrane proteins with an extracellular receptor domain and participate in biotic and abiotic stress responses.

Results: In this study, we identified RLKs in 15 representative plant genomes, including wheat, and classified them into 64 subfamilies by using four types of phylogenetic trees and HMM models.

Feline Panleukopenia Virus With G299E Substitution in the VP2 Protein First Identified From a Captive Giant Panda in China.

A feline panleukopenia virus (FPV), Giant panda/CD/2018, was isolated from a captive giant panda with mild diarrhea in 2018 in Chengdu, China, and further identified indirect immunofluorescence assay (IFA), transmission electron microscopy (TEM) observation, and genetic analysis. Phylogenetic analysis based on the complete VP2 nucleotide sequences showed that it shared high homology with Chinese FPV isolates and grouped within FPV cluster 1. One unique substitution Gly(G)299Glu(E) in the capsid protein VP2 was first identified with Giant panda/CD/2018.

An improved calibration and uncertainty analysis approach using a multicriteria sequential algorithm for hydrological modeling.

Hydrological models are widely used as simplified, conceptual, mathematical representatives for water resource management. The performance of hydrological modeling is usually challenged by model calibration and uncertainty analysis during modeling exercises. In this study, a multicriteria sequential calibration and uncertainty analysis (MS-CUA) method was proposed to improve the efficiency and performance of hydrological modeling with high reliability.

Projected soil organic carbon loss in response to climate warming and soil water content in a loess watershed.

Background: Soil organic carbon (SOC) plays a crucial role in the global carbon cycle and terrestrial ecosystem functions. It is widely known that climate change and soil water content (SWC) could influence the SOC dynamics; however, there are still debates about how climate change, especially climate warming, and SWC impact SOC. We investigated the spatiotemporal changes in SOC and its responses to climate warming and root-zone SWC change using the coupled hydro-biogeochemical model (SWAT-DayCent) and climate scenarios data derived under the three Representative Concentration Pathways (RCPs2.

Characteristics of Chimeric West Nile Virus Based on the Japanese Encephalitis Virus SA14-14-2 Backbone.

West Nile virus disease (WND) is an arthropod-borne zoonosis responsible for nonspecific fever or severe encephalitis. The pathogen is West Nile virus belonging to the genus family . Every year, thousands of cases were reported, which poses significant public health risk.

Development of recombinase polymerase amplification assays for rapid and visual detection of canine distemper virus infecting giant panda.

Background: Canine distemper virus (CDV) is an enveloped negative-strand RNA virus that exhibits a high mutation rate and continuously expands the range of hosts. Notably, CDV has infected giant panda with spill over from viral reservoirs in canines. Giant pandas (Ailuropoda melanoleuca), especially captive pandas, are known to be susceptible to natural infection with CDV.

A highly efficient recombinant canarypox virus-based vaccine against canine distemper virus constructed using the CRISPR/Cas9 gene editing method.

Canine distemper virus (CDV) is the causative agent of canine distemper (CD), which is one of the most important infectious diseases affecting wild and domestic carnivores. Vaccination represents an effective approach to prevent CDV infection among domestic carnivores. Canarypox-vectored recombinant CD vaccines (such as Recombitek CDV, PureVax Ferret Distemper, and Merial) with the CDV hemagglutinin (H) and fusion (F) genes can induce a potent immune response in dogs and ferrets.

Characteristics of land-atmosphere interaction parameters in hinterland of the Taklimakan Desert.

The importance of the energy exchange between the land surface and the atmosphere can be characterized by bulk transfer coefficients for momentum, C, and heat, C. The diurnal and monthly variations of both bulk transfer coefficients and lengths of surface roughness are analyzed. Based on observed data from January to December 2009 in hinterland of the Taklimakan Desert, the characteristics of aerodynamic roughness length, z, and thermal roughness length, z, are discussed.

Long-term spatio-temporal precipitation variations in China with precipitation surface interpolated by ANUSPLIN.

Climate changes significantly impact environmental and hydrological processes. Precipitation is one of the most significant climatic parameters and its variability and trends have great influences on environmental and socioeconomic development. We investigate the spatio-temporal variability of precipitation occurrence frequency, mean precipitation depth, PVI and total precipitation in China based on long-term precipitation series from 1961 to 2015.

High-resolution simulation and validation of soil moisture in the arid region of Northwest China.

Soil moisture plays an important role in land-atmosphere interactions, agricultural drought monitoring, and water resource management, particularly across arid regions. However, it is challenging to simulate soil moisture of high spatial resolution and to evaluate soil moisture at fine spatial resolution in arid regions in Northwest China due to considerable uncertainties in forcing data and limited in situ measurements. Then, the data set was used to produce the 1 km high-resolution atmospheric forcing datasets and to drive the Community Land Model version 3.

Retraction Note: Simulation and spatiotemporal pattern of air temperature and precipitation in Eastern Central Asia using RegCM.

This paper has been retracted.

Seasonal Characteristics and Particle-size Distributions of Particulate Air Pollutants in Urumqi.

Urban particulate air pollution is a known cause of adverse human health effects worldwide. Urumqi is a large oasis city in which rapid urbanization has caused a series of eco-environmental problems including serious air pollution, water shortage, dense population, excess energy consumption, and the creation of an urban heat island, among others. Coal is the most important source of energy and air pollutants that are poorly dispersed into the natural surroundings are the main reasons for serious pollution in the Urumqi urban area.

Simulation and spatiotemporal pattern of air temperature and precipitation in Eastern Central Asia using RegCM.

Central Asia is a region that has a large land mass, yet meteorological stations in this area are relatively scarce. To address this data issues, in this study, we selected two reanalysis datasets (the ERA40 and NCEP/NCAR) and downscaled them to 40 × 40 km using RegCM. Then three gridded datasets (the CRU, APHRO, and WM) that were extrapolated from the observations of Central Asian meteorological stations to evaluate the performance of RegCM and analyze the spatiotemporal distribution of precipitation and air temperature.

Study on the characteristics of future precipitation in response to external changes over arid and humid basins.

The simulation abilities of the Coupled Model Inter-comparison Project Phase 5 (CMIP5) models to the arid basin (the Tarim River Basin, TRB) and humid basin (the Yangtze River Basin, YRB) were evaluated, determining the response of precipitation to external changes over typical basins. Our study shows that the future temporal and spatial variation characteristics of precipitation are different in different regions with the CMIP5. The annual and seasonal changes in precipitation were analyzed for the RCP2.

Investigating spatiotemporal changes of the land-surface processes in Xinjiang using high-resolution CLM3.5 and CLDAS: Soil temperature.

Soil temperature plays a key role in the land surface processes because this parameter affects a series of physical, chemical, and biological processes in the soil, such as water and heat fluxes. However, observation of soil temperature is quite limited, especially at the regional scale. Therefore, this study is to investigate the spatiotemporal features of soil temperature in Xinjiang, China, using the Community Land model 3.

Pre-rigor temperature control of Chinese yellow cattle carcasses to 12-18 °C during chilling improves beef tenderness.

This study evaluates the effects of pre-rigor temperature control on quality traits of Chinese yellow cattle M. longissimus lumborum (LL). One stepwise chilling (SC) treatment was used on one half-carcass, involved a fast chilling (-11 ± 1 °C;0.

[Effect of internal fixation on stability of pedicled fascial flap and osteogenesis of exceed critical size defect of bone].

Objective: To evaluate the effect of internal fixation on the stability of pedicled fascial flap and the osteogenesis of exceed critical size defect (ECSD) of bone so as to provide theory for the clinical application by the radiography and histology observation.

Methods: The ECSD model of the right ulnar midshaft bone and periosteum defect of 1 cm in length was established in 32 New Zealand white rabbits (aged 4-5 months), which were divided into group A and group B randomly (16 rabbits in each group). The composite tissue engineered bone was prepared by seeding autologous red bone marrow (ARBM) on osteoinductive absorbing material (OAM) containing bone morphogenetic protein and was used repair bone defect.

Osthole improves synaptic plasticity in the hippocampus and cognitive function of Alzheimer's disease rats via regulating glutamate.

Osthole, an effective monomer in Chinese medicinal herbs, can cross the blood-brain barrier and protect against brain injury, with few toxic effects. In this study, a rat model of Alzheimer's disease was established after intracerebroventricular injection of β-amyloid peptide (25-35). Subsequently, the rats were intraperitoneally treated with osthole (12.

[Clinical evaluation of one-stage radical debridement combined with posterior pedicle internal fixation for brucellar spondylitis of the thoracic and lumbar vertebrae].

Objective: To discuss the surgical treatment method and effectiveness of brucellar spondylitis.

Methods: Between January 2002 and October 2010, 78 patients underwent one-stage radical debridement and posterior pedicle internal fixation, who were definitely diagnosed as having brucellar spondylitis of the thoracic and lumbar vertebrae. There were 42 males and 36 females, aged 24 to 65 years (mean, 45 years).

[Feasibility and clinical application of design about the hollowed titanium stick supporting the femoral head and preventing it from collapsing].

Objective: To evaluate the feasibility and the clinical practicality of the design about the hollowed titanium stick supporting the femoral head and preventing it from collapsing.

Methods: From Jan.2003 to Jun.

[Comparison of effect between vascularization osteogenesis and membrane guided osteogenesis in bone repair by tissue engineered bone with pedicled fascial flap packing autologous red bone marrow].

Objective: To compare the effect between vascularization osteogenesis and membrane guided osteogenesis in the bone repair by the tissue engineered bone with pedicled fascial flap packing autologous red bone marrow (ARBM), so as to provide a reference for the bone defect repair in clinic.

Methods: The tissue engineered bone was constructed with ARBM and the osteoinductive absorbing recombinant human materials with recombinant human bone morphogenetic protein 2. Sixty New Zealand rabbits (aged 4-5 months, weighing 2.