Polydeoxyribonucleotide-spermidine nanoparticles: Preparation, characterization, and verification of skin anti-aging efficacy.

Regenerative medicine, with stem cells and tissue engineering as its core, is dedicated to the regeneration of skin and organs and the treatment of diseases. Polydeoxyribonucleic acid (PDRN) has been demonstrated to promote skin regeneration and anti-aging in the field of regenerative medicine, yet its application is constrained by its inherent characteristics, including its high electronegativity, poor transdermal delivery, low transmembrane and susceptibility to degradation. Spermidine is a trivalent molecule with a variety of biological activities, including autophagy induction, cell regeneration and free radical scavenging.

Coordination of distinct sources of excitatory inputs enhances motion selectivity in the mouse visual thalamus.

Multiple sources innervate the visual thalamus to influence image-forming vision prior to the cortex, yet coordination between non-retinal and retinal inputs in shaping thalamic visual selectivity remains unclear. Using dual-color two-photon calcium imaging in the thalamus of awake mice, we observed a higher fraction of direction-selective boutons among input from superior colliculus neurons than from retinal ganglion cells, both providing strong converging excitatory input to thalamic neurons. Collicular and retinal axons exhibit retinotopic organization with similar precision.

Use of integrated spatial transcriptomics and histopathological analysis in adamantinomatous craniopharyngiomas to identify stromal cells as a new cellular source of leukemia inhibitory factor.

Objective: Adamantinomatous craniopharyngioma (ACP) is an epithelial tumor that occurs in the sellar region of the human brain. Despite resection, relapse is frequent with poor prognosis. To facilitate the development of new therapy for ACP, the authors examined the spatial distribution, cell of origin, and potential biological functions of leukemia inhibitory factor (LIF), an important stem cell self-renewal regulator, in a series of ACP tumors.

The relationship between dark traits and cyberbullying behavior among vocational school students: a moderated mediation model.

Background: The General Aggression Model offers the theoretical basis for this study, which evaluates connections among Dark Traits, Social Anxiety, School Climate, and Cyberbullying. This analysis centers on Chinese vocational school students, a demographic infrequently studied as an integrated system. This research aims to clarify the mediating role of social anxiety in the relationship between dark personality and cyberbullying, as well as the moderating role of school climate.

Effects of auditory training on children with developmental language disorder: a systematic review.

Objective: This systematic review aims to evaluate the effectiveness of auditory training (AT) on various parameters, including language abilities, speech perception, auditory behavior, electrophysiological assessments, and working memory, in children with developmental language disorder (DLD) population.

Methods: We searched PubMed, Web of Science, EMBASE, MEDLINE, Cochrane Library and CINAHL from inception to August 7, 2023, and further scrutinized the references of all selected articles. We included randomized controlled trials (RCTs) and quasi-experimental studies that investigated the effects of AT on children with DLD.

Cryo-EM structures reveal the PP2A-B55α and Eya3 interaction that can be disrupted by a peptide inhibitor.

We have previously shown that Eya3 recruits PP2A-B55α to dephosphorylate pT58 on Myc, increasing Myc stability and enhancing primary tumor growth of triple-negative breast cancer (TNBC). However, the molecular details of how Eya3 recruits PP2A-B55α remain unclear. Here, we determined the cryo-EM structures of PP2A-B55α bound with Eya3, with an inhibitory peptide B55i, and in its unbound state.

Identifying environmental chemicals impacting neurodevelopment using a random mixture-based screening approach.

Environmental exposures can impact brain development and contribute to neurodevelopmental disorder risk. In this study, we leveraged insights from in vitro high-throughput screening studies that examined the developmental toxicity of environmental chemicals to select 47 chemicals for in vivo testing as complex random mixtures in pregnant female mice. Our objectives were to identify mixtures that impact key neurodevelopmental endpoints-embryonic body, brain, and placenta weight-and subsequently to use mass spectrometry to ascertain which chemicals from each active mixture entered the developing brain following gestational exposure.

Regulatory role of the Bxy-gld-1 in meiosis and oocyte development of Bursaphelenchus xylophilus.

Background: Investigating the cell fate of the germline in Bursaphelenchus xylophilus can reveal the biological characteristics of its reproductive processes, which is crucial for formulating more targeted control strategies against pine wilt disease. However, the molecular regulatory mechanisms governing cell fate in the germline of B. xylophilus remain unclear.

[Family socioeconomic status and children's reading fluency: the chain mediating role of family reading environment and children's living and learning styles].

Objectives: To study the impact of family socioeconomic status on children's reading fluency and the chain mediation effect of family reading environment and children's living and learning styles in this relationship.

Methods: A total of 473 children from grades 2 to 6 in two primary schools in Nanjing were selected through stratified random sampling. The children's reading fluency was assessed, and a questionnaire was used to collect information on family socioeconomic status, family reading environment, and children's living and learning styles.

Anti-Inflammatory Combination of Puerarin and Ac2-26 Using Intranasal Delivery for Effective Against Ischemic Stroke in Rat Model.

Purpose: The pathological mechanisms underlying ischemic stroke are highly complex, with the neuroinflammatory response triggered by cerebral ischemia-reperfusion being a major contributor to secondary brain damage. This response significantly impedes neural tissue regeneration. Despite advancements in treatment, current anti-inflammatory strategies remain suboptimal in terms of safety and efficacy.

Ulvan Microneedles Loaded with Photosensitizer 5-aminolevulinic Acid Inhibits Human Cervical Cancer HeLa Cells .

Background: Cervical cancer encompasses highly invasive and metastatic malignant tumors with poor prognoses. Recently, microneedles have gained significant attention as a novel, non-invasive drug delivery method, offering unique advantages in tumor treatment.

Objective: This study aims to develop an ulvan-based microneedle delivery system encapsulating the photosensitizer 5-aminolevulinic acid (5-ALA-UMNs) and to investigate its inhibitory effects on the growth of human cervical cancer Hela cells.

Cryo-EM structures reveal the PP2A-B55α and Eya3 interaction that can be disrupted by a peptide inhibitor.

We have previously shown that Eya3 recruits PP2A-B55α to dephosphorylate pT58 on Myc, increasing Myc stability and enhancing primary tumor growth of triple-negative breast cancer (TNBC). However, the molecular details of how Eya3 recruits PP2A-B55α remain unclear. Here we determined the cryo-EM structures of PP2A-B55α bound with Eya3, with an inhibitory peptide B55i, and in its unbound state.

Coordination of distinct sources of excitatory inputs enhances motion selectivity in the mouse visual thalamus.

Unlabelled: Multiple sources innervate the visual thalamus to influence image-forming vision prior to the cortex, yet it remains unclear how non-retinal and retinal input coordinate to shape thalamic visual selectivity. Using dual-color two-photon calcium imaging in the thalamus of awake mice, we observed similar coarse-scale retinotopic organization between axons of superior colliculus neurons and retinal ganglion cells, both providing strong converging excitatory input to thalamic neurons. At a fine scale of ∼10 µm, collicular boutons often shared visual feature preferences with nearby retinal boutons.

Principles of CRISPR-Cas13 mismatch intolerance enable selective silencing of point-mutated oncogenic RNA with single-base precision.

Single-nucleotide variants (SNVs) are extremely prevalent in human cancers, although most of these remain clinically unactionable. The programmable RNA nuclease CRISPR-Cas13 has been deployed to specifically target oncogenic RNAs. However, silencing oncogenic SNVs with single-base precision remains extremely challenging due to the intrinsic mismatch tolerance of Cas13.

A three-phase strategy of bionic drug reservoir scaffold by 3D printing and layer-by-layer modification for chronic relapse management in traumatic osteomyelitis.

We have developed a novel three-phase strategy for osteomyelitis treatment, structured into three distinct phases: the "strong antimicrobial" phase, the "monitoring and osteogenesis" phase and the "bone repair" phase. To implement this staged therapeutic strategy, we engineered a bionic drug reservoir scaffold carrying a dual-drug combination of antimicrobial peptides (AMPs) and simvastatin (SV). The scaffold integrated a bilayer gel drug-carrying structure, based on an induced membrane and combined with a 3D-printed rigid bone graft using a layer-by-layer modification strategy.

Digital technology and psychological happiness: the mediating roles of interpersonal relationships and employment situations.

Psychological happiness represents the ultimate pursuit of human beings, and the impact of digital technology on psychological happiness is becoming increasingly significant in the era of the digital economy. Based on data from 2020 China Family Panel Studies (CFPS), this study constructs an empirical model that examines the effect and mechanism of digital technology on happiness. Additionally, this study investigates the heterogeneity and robustness of the impact of digital technology on happiness.

Synthesis and Biological Evaluation of Novel Piperidine-3-Carboxamide Derivatives as Anti-Osteoporosis Agents Targeting Cathepsin K.

A series of novel piperidamide-3-carboxamide derivatives were synthesized and evaluated for their inhibitory activities against cathepsin K. Among these derivatives, compound exhibited the most potent inhibition, with an IC value of 0.08 µM.

Effect of Extrusion Process on Microstructure, Corrosion Properties, and Mechanical Properties of Micro-Alloyed Mg-Zn-Ca-Zr Alloy.

The effect of the extrusion process on the microstructure, corrosion, and mechanical properties of Mg-Zn-Ca-Zr alloy has been investigated. Zn and Ca were both in a solid solution and only the Zr-rich phase was observed in the homogenized and extruded alloys. The Zr-rich phase was obviously refined after extrusion.

Effect of Ce Addition on Microstructure, Thermal Conductivity, and Mechanical Properties of As-Cast and As-Extruded Mg-3Sn Alloys.

In the present research, the impacts of Ce additions at various concentrations (0, 1.0, 3.4, and 4.

CXCR4 promotes migration, invasion, and epithelial-mesenchymal transition of papillary thyroid carcinoma by activating STAT3 signaling pathway.

Aims: Papillary thyroid cancer (PTC) is a serious threat to human health worldwide, while metastasis in the early phase limits therapeutic success and leads to poor survival outcomes. The CXC chemokine receptor type 4 (CXCR4) plays an important role in many cellular movements such as transcriptional modulation, cell skeleton rearrangement, and cell migration, and the change in CXCR4 levels are crucial in various diseases including cancer. In this study, we explored the role of CXCR4 in the migration and invasion of PTC and investigated the potential mechanisms underlying its effects.

Impact of Combined Pollution of Ciprofloxacin and Copper on the Diversity of Archaeal Communities and Antibiotic-Resistance Genes.

This study aimed to explore the response of archaeal communities and antibiotic-resistance genes (ARGs) to ciprofloxacin (CIP, 0.05-40 mg/L) and copper (Cu, 3 mg/L) combined pollution during stress- and post-effect periods in an activated sludge system. With the increase in the CIP concentration, the diversity of archaea decreased, but the richness increased under the stress of 10 mg/L CIP.

Development of PainFace software to simplify, standardize, and scale up mouse grimace analyses.

Facial grimacing is used to quantify spontaneous pain in mice and other mammals, but scoring relies on humans with different levels of proficiency. Here, we developed a cloud-based software platform called PainFace ( http://painface.net ) that uses machine learning to detect 4 facial action units of the mouse grimace scale (orbitals, nose, ears, whiskers) and score facial grimaces of black-coated C57BL/6 male and female mice on a 0 to 8 scale.

Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing.

The development of precise RNA-editing tools is essential for the advancement of RNA therapeutics. CRISPR (clustered regularly interspaced short palindromic repeats) PspCas13b is a programmable RNA nuclease predicted to offer superior specificity because of its 30-nucleotide spacer sequence. However, its design principles and its on-target, off-target and collateral activities remain poorly characterized.

Association between serum neuron-specific enolase at admission and the risk of delayed neuropsychiatric sequelae in adults with carbon monoxide poisoning: A meta-analysis.

Delayed neuropsychiatric sequelae (DNS) significantly impact the quality of life in patients following acute carbon monoxide poisoning (COP). This systematic review and meta-analysis aimed to assess the relationship between serum neuron-specific enolase (NSE) levels at admission and the risk of DNS in adults after acute COP. Relevant observational studies with longitudinal follow-up were identified through searches in PubMed, Embase, Web of Science, Wanfang, and China National Knowledge Infrastructure databases.