Diagnostic and prognostic roles of interferon-λ1 and interferon-λ3 in bronchoalveolar lavage fluid and plasma in non-neutropenic patients with invasive pulmonary aspergillosis.

This study aims to evaluate the diagnostic and prognostic value of interferon (IFN)-λ1 and IFN-λ3 levels in bronchoalveolar lavage fluid (BALF) and plasma in non-neutropenic invasive pulmonary aspergillosis (IPA) patients. A total of 481 patients suspected of IPA were enrolled (169 IPA cases and 312 non-IPA cases) in this study. BALF and plasma samples were collected, and IFN-λ1 and IFN-λ3 levels were measured using an enzyme-linked immunosorbent assay.

Microvesicle-transferred mitochondria trigger cGAS-STING and reprogram metabolism of macrophages in sepsis.

The inflammatory cytokine storm is a hallmark of sepsis and is highly correlated with organ injury. Therefore, inhibiting inflammatory cytokine production is a straightforward strategy for effectively treating this disease. In this study, we found that microvesicles from lipopolysaccharide (LPS)-primed macrophages could transfer mitochondria to other macrophages and alter their biological functions.

Induced nanostructured MnO on carbon cloth with optimized sodium-ion storage capability for self-supporting supercapacitors.

Herein, a hybrid-structured MnO cathode with self-supporting carbon cloth (CI-MnO@CC) was constructed by a nanomicellar-induced strategy cetyltrimethylammonium bromide for advanced supercapacitors. This self-supporting electrode achieves enhanced Na-ion storage ability and structure stability, which provides new insights for designing functional MnO electrodes for high-performance supercapacitors.

CPNE5 overexpression inhibits cardiomyocytes apoptosis by promoting the degradation of FAS receptor.

CPNE5, a member of the Copine family, is characterized by its membrane-binding properties and functions as a regulatory modulator of intracellular signaling through the spatial redistribution of interacting protein partners. Emerging evidence has demonstrated that CPNE3 exerts cardioprotective effects via anti-apoptotic activity in myocardial ischemia-reperfusion injury models. However, the functional role of CPNE5 in cardiac pathology remains unclear.

Quantitative assessment of lumbar intervertebral disc degeneration and its correlation with clinical symptoms: a study utilizing ultrashort time-of-echo and T2 mapping as biomarkers.

Background: Lumbar cartilage endplate (CEP) structures show low signal intensity on conventional magnetic resonance imaging (MRI), making them hard to observe and quantify. This often results in poor correlation between conventional MRI findings and low back pain (LBP) symptoms and provides inadequate guidance for clinical decisions.

Methods: The study included Twenty-five healthy volunteers and forty-one patients with LBP.

Enhanced One-Pot Cas12a-Based Nucleic Acid Detection via Epitope Insertion and Recruitment of Rad51.

The CRISPR-Cas12a system has emerged as a promising tool for nucleic acid-based diagnostics. However, its multi-step workflow and limited sensitivity hinder its integration into point-of-care testing (POCT). Here, the ECOT system (Engineered Cas12a for One-pot Test), a novel approach that combines protein engineering with one-pot detection, offering high sensitivity, specificity, and rapid response is introduced.

Disassembly of chiral hydrogen-bonded frameworks into single-unit organometallic helices for enantioselective amyloid inhibition.

Chiral nanostructures hold transformative potential across diverse fields, yet their assembly construction remains hindered by the high entropic barrier of dissymmetric building units. Inspired by biological structural dynamics, we construct two chiral copper-based hydrogen-bonded frameworks [D(L)-Cu-crystals] via hydrogen-bonded assembly using chiral metal-organic helical as the building unit. Single-crystal X-ray diffraction elucidates hierarchical chirality evolution from asymmetric coordinations to helical chains and framework packing.

Engineered Stem Cell-Derived Exosomes for Dual siRNA and Chemotherapy Delivery via Rolling Circle Transcription.

Exosomes (EVs) are promising drug carriers, with efficiency dependent on membrane proteins and drug loading capacity. Preserving EVs integrity and enabling efficient drug loading is crucial. We developed a nondestructive system using stem cell-derived EVs for tumor treatment by functionalizing the EVs surface with a cholesterol-modified T7 primer, enabling robust siRNA production via rolling circle transcription (RCT) for potent gene silencing.

PIEZO1-Mediated Mechanotransduction in Craniofacial Biology.

PIEZO1, a critical mechanosensitive ion channel, has been demonstrated to convert mechanical forces into biochemical signals via Ca influx, thereby regulating pathways such as Wnt/β-catenin, NF-κB, and YAP/TAZ. It plays a pivotal role in craniofacial biology by maintaining bone homeostasis, facilitating dentin mineralization, periodontal remodeling, and orthodontic tooth movement. Expressed in bone tissue, dental pulp stem cells, and periodontal ligament cells, dysregulated PIEZO1 is implicated in a number of conditions, including osteoporosis, dentin hypersensitivity, temporomandibular disorders, and periodontitis.

The clinical value of serum sST2 and cfDNA in guiding evidence-based nursing care for children with severe pneumonia complicated by myocardial damage.

Background: To investigate how serum sST2 and cfDNA can be used to inform evidence-based nursing practices for children with severe pneumonia and myocardial damage.

Methods: 100 children with severe pneumonia complicated with myocardial damage were recruited as research subjects. After assessing serum sST2 and cfDNA concentrations, the individuals were categorised into a control cohort (receiving standard treatment, n=50) and an experimental cohort (receiving evidence-based treatment guided by serum sST2 and cfDNA markers, n=50).

High-throughput single-cell analysis reveals fully human Omp38-specific monoclonal antibodies against Acinetobacter baumannii.

Background: Acinetobacter baumannii (A. baumannii) is a significant global health threat, particularly in hospital environments, where it is often linked to severe infections. As the need for innovative therapeutic approaches grows, fully human monoclonal antibodies (mAbs) have gained attention because of their high specificity, reduced immunogenicity, and enhanced affinity for target antigens, which may improve clinical efficacy.

Lightweight remote sensing change detection with progressive multi scale difference aggregation.

Change detection (CD) is the process of acquiring changes in the ground surface by analyzing remotely sensed images of the same location at two different stages. Deep learning is becoming increasingly popular for change detection tasks in remote sensing due to its significant advantages in deep feature representation and nonlinear problem modeling. However, many previous neural network-based approaches require a large number of parameters and computations and high-performance hardware, which makes their practical application in remote sensing challenging.

Constitutive Androstane Receptor in Macrophages Regulates Toll-Like Receptor 4-Mediated Innate Immune Responses Against Endotoxemic Liver Injury.

Macrophage proinflammatory hyperactivation drives the pathogenesis of acute liver injury, a common complication of sepsis. The role of the nuclear receptor constitutive androstane receptor (CAR) in endotoxin-induced liver injury remains unclear. Here, this study reports that CAR is highly expressed in human and murine macrophages.

Immune stratification in HPV-negative HNSCC: prognostic significance and prediction of immunotherapy response.

The tumor immune microenvironment (TIME) played a crucial role in head and neck squamous cell carcinoma (HNSCC), significantly impacting disease progression and immunotherapy. To address these complexities, we analyzed gene expression profiles of 790 HNSCC tumors by combining data from The Cancer Genome Atlas (TCGA; n = 520) and the Gene Expression Omnibu database (GEO, n = 270). We identified five distinct TIME-based phenotypes, with the E class-characterized by tertiary lymphoid structures (TLS)-showing the highest survival rates and immunotherapy responses.

Tumor-targeted nano-delivery system of camptothecin nanocrystals for the effective treatment of triple negative breast cancer.

Clinical studies have shown that camptothecin and its derivatives have good antitumor activity for triple negative breast cancer (TNBC). However, the poor water solubility and low therapeutic selectivity limited their use in the clinic. Drug delivery systems are of great significance in enhancing drug solubility and precisely controlling drug release, thus increasing the therapeutic efficacy and reducing the toxicity.

Identification of a novel SALL4 variant associated with unilateral renal agenesis and right renal pelvis duplication by prenatal exome sequencing: a case report.

Congenital renal anomalies are one of the leading causes of perinatal and neonatal mortality in children. Here, we present a case of a 17-week-6-day pregnant patient, in whom prenatal ultrasound confirmed the fetal right duplex kidney and left renal agenesis, leading to termination of pregnancy at the patient's request. Whole-exome sequencing were conducted on the fetus and its parents to identify the cause of the fetal ultrasound abnormalities, followed by validation with Sanger sequencing and CMA/SNP-Array.

Decreased Serum Adipose Triglyceride Lipase Level Is Associated With Renal Function Impairment in Patients With Type 2 Diabetes.

Diagnosing diabetic kidney disease (DKD) remains a significant challenge. Research has increasingly focused on kidney injury resulting from lipid metabolism disorders. Adipose triglyceride lipase (ATGL), a pivotal enzyme in lipolysis, is essential for maintaining lipid metabolism balance.

From operating room to recovery: Evidence and gaps in cardiac surgical nursing integrative psychological support.

This review explored the application and effectiveness of and research gaps in integrative psychological support in patients undergoing cardiac surgery during the perioperative period. These patients often encounter psychological issues such as anxiety and depression, which affect their postoperative recovery and quality of life. Previous research indicated that preoperative psychological assessments and interventions significantly enhance patients' psychological states and postoperative outcomes.

Successful Treatment of Mucormycosis and Azole-Resistant Coinfection in a Diabetic Patient: A Case Report.

Objective: To report a case of coinfection with mucormycosis and azole-resistant in a patient with poorly controlled type 2 diabetes, highlighting successfully treated with high-dose posaconazole combined with liposomal amphotericin B and lobectomy.

Methods: A 52-year-old man who presented to our hospital with a 1-month history of fever accompanied by productive cough and sputum. He had type 2 diabetes with poor control of glucose level.

Stable Synaptic Transistor-Based Humidity Sensor with Broad Detection Window.

Human visual recognition is profoundly affected by ambient relative humidity, yet current bionic and neuromorphic systems lack the ability to adapt to environmental variability, resulting in mismatches between human and robotic perception. In this work, a stable humidity-sensitive synaptic transistor featuring a broad detection window is designed and fabricated to bridge the gap between human and robotic sensory capabilities. The proposed humidity sensory neuron integrates a humidity sensing unit with a synaptic transistor in a separation device structure, enabling independent optimization of sensing and neuromorphic functions.

Is adenotonsillar hypertrophy associated with dentofacial morphology? A systematic review and meta-analyses.

Introduction: As a common cause of upper airway obstruction in children, adenotonsillar hypertrophy (ATH) has been hypothesized to adversely affect dentofacial development and morphology. This systematic review aimed to summarize the existing evidence regarding the association between ATH and dentofacial characteristics of children.

Methods: Four databases (PubMed, Embase, Web of Science, and VIP Chinese Journal Database) were searched from inception to November 1, 2024, for cross-sectional studies that compared the dental or craniofacial characteristics of children with and without adenoid hypertrophy (AH) and/or tonsillar hypertrophy (TH).

Two DNA-binding One Zinc Finger transcription factors, MdCDOF3 and MdDOF3.6, accelerate leaf senescence by activating in response to sorbitol signaling in apple.

Leaf senescence, an essential component of the plant life cycle, seriously affects the productivity of numerous commercial crops, with cytokinins serving as crucial regulators in delaying this process. Here, we observed that apple () leaves exhibiting deficiencies in sorbitol synthesis due to antisense inhibition of () presented an increase in cytokinin content and exhibited a delay in leaf senescence, in contrast to wild-type (WT) leaves. Transcriptome analysis indicated that the expression of (), encoding a key enzyme in the cytokinin degradation pathway, was significantly downregulated in the antisense lines.

Decision level scheme for fusing multiomics and histology slide images using deep neural network for tumor prognosis prediction.

Molecular biostatistical workflows in oncology often rely on predictive models that use multimodal data. Advances in deep learning and artificial intelligence technologies have enabled the multimodal fusion of large volumes of multimodal data. Here, we presented a decision level multimodal data fusion framework for integrating multiomics and pathological tissue slide images for prognosis prediction.

Nuclear Receptor ERRγ Protects Against Cardiac Ischemic Injury by Suppressing GBP5-Mediated Myocardial Inflammation.

Myocardial inflammation plays a critical role in the progression of injury following myocardial infarction (MI), yet the transcriptional mechanisms regulating cardiomyocyte inflammation to mitigate post-ischemic injury remain poorly understood. This study elucidated the role of Estrogen-Related Receptor Gamma (ERRγ) in modulating the inflammatory response post-MI, demonstrating that ERRγ expression was downregulated in ischemic tissue and hypoxic neonatal mouse ventricular myocytes (NMVMs). Cardiomyocyte-specific overexpression of ERRγ reduced infarct size, improved cardiac function, and suppressed excessive myocardial inflammation and pyroptosis by binding to the GBP5 promoter, thereby inhibiting GBP5 transcription and reducing NLRP3 inflammasome assembly.