Tracing the evolution of single-cell 3D genomes in Kras-driven cancers.

Although three-dimensional (3D) genome structures are altered in cancer, it remains unclear how these changes evolve and diversify during cancer progression. Leveraging genome-wide chromatin tracing to visualize 3D genome folding directly in tissues, we generated 3D genome cancer atlases of oncogenic Kras-driven mouse lung adenocarcinoma (LUAD) and pancreatic ductal adenocarcinoma. Here we define nonmonotonic, stage-specific alterations in 3D genome compaction, heterogeneity and compartmentalization as cancers progress from normal to preinvasive and ultimately to invasive tumors, discovering a potential structural bottleneck in early tumor progression.

Identification of genes and lncRNAs for intramuscular and subcutaneous fat deposition in ducks by transcriptome analysis.

The purpose of this study was to identify key lncRNAs and mRNAs involved in fat deposition of meat ducks based on whole transcriptome sequencing for intramuscular preadipocyte (IMP-0), intramuscular adipocyte after 4 days of induction (IMP-4), subcutaneous preadipocyte (SCP-0), and subcutaneous adipocyte after 4 days of induction (SCP-4). We found 1419 and 697 differentially expressed genes and lncRNAs in the IMP-0-vs-IMP-4 group, respectively. There were 2307 and 1180 differentially expressed genes and differential lncRNAs, respectively, in the SCP-0-vs-SCP-4 group.

Global, regional, and national burden of cataract: A comprehensive analysis and projections from 1990 to 2021.

Objective: Cataract is the most prevalent cause of blindness. Surgery remains the only effective and widely accepted treatment; early diagnosis and intervention can significantly prevent blindness. Hence,Understanding the current epidemiological status of cataract is crucial for formulating better healthcare policies and effectively preventing blindness due to cataract.

Exploring the effects of audiovisual incongruence on working memory performance in the combined 2-back+ Go/NoGo paradigm.

Introduction: The human brain processes 83% of information visually and 11% auditorily, with visual perception dominating multisensory integration. While audiovisual congruence enhances cognitive performance, the impact of audiovisual incongruence on working memory (WM) remains controversial. This study investigated how audiovisual incongruence affects WM performance under varying cognitive loads.

Multifunctional and Radii-Matched High-Entropy Engineering Toward Locally-Regulable Metal Oxide Layers in Sodium-Layered Oxide Cathode.

Layered oxides, one of the most fascinating cathodes for sodium-ion batteries (SIBs), have appropriate voltage window and feasible preparation process, however, cycling stability is the biggest challenge. Element doping is the most rational strategy to address this problem, but six-coordinated octahedral radii and different radii in different valence states of these doping elements and the functions of these elements need to be taken into account. Hence, an example of P2/O3-type NaMnNiFeMgVCoCuZnSnO (high-entropy-doped layered oxides, HEO) has been designed in consideration of moderate six-coordinated octahedral radii and stabling the metal-oxygen bond.

Sulfur modification enhances promotion of carbon-iron composites on carbon chain elongation.

Efficient synthesis of caproate is crucial for the recovery of organic wastes. However, the yield and selectivity of caproate are limited by the efficiency of carbon chain elongation (CCE). This study presents sulfur-modified carbon-iron composites (BC[S-Fe]) as an innovative approach to enhance CCE for efficient caproate biosynthesis.

Construction of Organic-Inorganic Solid Electrolyte Interphase by Gas-Liquid Plasma for High Performance Lithium Metal Anodes.

The construction of high-quality solid electrolyte interphase (SEI) on Li metal is one of the key strategies to improve the performance of Li metal anodes. Herein, we propose a novel gas-liquid hybrid source plasma technology to construct composite SEI consisting of organic lithium methyl carbonate (LMC) and inorganic lithium nitride (LiN) and lithium oxide (LiO) on the lithium metal. Supported by the theoretical calculation, the inorganic LiN and LiO phases possess low diffusion barrier potentials, favorable for fast Li transportation, and enhanced lithophilicity.

Astrocytic mGluR5-dependent calcium hyperactivity promotes amyloid-β pathology and cognitive impairment.

Astrocytic dysfunction is a crucial factor for the pathogenesis of Alzheimer's disease. Metabotropic glutamate receptor 5 (mGluR5) is ubiquitously expressed in the brain and is a key molecule that regulates synaptic transmission and plasticity. It has been shown that mGluR5 is elevated in astrocytes in Alzheimer's disease.

Perturb-tracing enables high-content screening of multi-scale 3D genome regulators.

Three-dimensional (3D) genome organization becomes altered during development, aging and disease, but the factors regulating chromatin topology are incompletely understood and currently no technology can efficiently screen for new regulators of multi-scale chromatin organization. Here, we developed an image-based high-content screening platform (Perturb-tracing) that combines pooled CRISPR screens, a cellular barcode readout method (BARC-FISH) and chromatin tracing. We performed a loss-of-function screen in human cells, and visualized alterations to their 3D chromatin folding conformations, alongside perturbation-paired barcode readout in the same single cells.

Innovative Analysis of the Interconnected Network Structure Between Anxiety and Sleep Quality Among College Students.

Background: A complex interplay exists between anxiety and sleep quality. However, there is a scarcity of network analysis studies examining this relationship, particularly among college students. Previous research has relied on sum scores from scales, which fails to capture the nuanced, symptom-level associations between anxiety and sleep quality.

Anti-corrosion lithium anode interface by LiLaZrTaO modified buffer layer for stable cycling of room-temperature solid-state lithium metal batteries.

Non-flammable butanedinitrile (SN) is recognized as a highly prospective plasticizer for significantly reducing the operating temperature of polyethylene oxide (PEO)-based solid polymer electrolytes. However, the instability of the lithium anode interface severely hinders the practical application of PEO/SN-based solid polymer electrolytes in room-temperature solid-state lithium metal batteries. In this work, we propose fast-ion conductive LiLaZrTaO (LLZTO) nanoparticles as corrosion inhibitors to constructure a multifunctional buffer layer on the surface of PEO/SN-based solid electrolyte (PSE@LLZTO) to stabilize the interface structure of Li anode via a facile spin-coating transfer technique.

Incidence and temporal trends of out-of-hospital cardiac arrest in Shenzhen, China (2011-2018).

Background: Out-of-hospital cardiac arrest (OHCA) is a significant global public health issue, few studies describe characteristics and trends in China. This study examines OHCA features and trends in Shenzhen, one of the fastest-growing cities.

Methods: This retrospective study analysed data from the Shenzhen Emergency Center database (2011-2018), including ambulance dispatch and pre-hospital medical records.

Temporal trends of presumed cardiac origin out-of-hospital cardiac arrest incidence in Guangzhou, southern China: A 10-year consecutive analysis.

Background: Despite the rising disease mortality rates, there is a dearth of studies on the incidence and trends of out-of-hospital cardiac arrests (OHCA) in China. This study aims to investigate the incidence and temporal trends of presumed cardiac origin OHCA in Guangzhou, Southern China, from 2011 to 2020.

Methods And Results: In this population-based retrospective cohort study, pre-hospital data from the Guangzhou Emergency Medical Service (GZ-EMS) from 2011 to 2020 were analyzed.

Stress and Interpersonal Relationships in Medical Students During Public Health Emergencies: A Network Analysis.

Background: With the growing demand for medical services worldwide, especially during public health emergencies, medical students, as a special group of future medical workers, are important for the development of health services, and they therefore experience more stress than ordinary college students do. The mental health of medical students plays a vital role in their training, and interpersonal relationships are an important factor affecting their stress levels.

Purpose: This study aims to investigate the network structure of stress and interpersonal relationships among Chinese medical students and identify bridge variables to prevent and relieve this stress.

3'UTR of tobacco vein mottling virus regulates downstream GFP expression and changes in host gene expression.

Introduction: Tobacco vein mottling virus (TVMV) is a member of the family . The 3' untranslated region (3'UTR) of viral genomic RNA has been reported to significantly impact viral infection. Nevertheless, the role of the TVMV 3'UTR during viral infection remains unknown.

Interface-reinforced high-capacity fiber cathode for wearable Li-S batteries.

Fiber-shaped Li-S batteries are attractive for constructing smart textiles as flexible power solutions due to their high theoretical specific capacity, flexibility and wearability. However, severe interfacial issues, such as the shuttle effect of polysulfides on the cathode side, lead to capacity decay and poor lifespan of the batteries. Herein, we report a fiber-shaped composite cathode with collaborative interface interactions to maintain electrode integrity and boost electrochemical performance.

Study on the Water Absorption Characteristics of Anthracite Particles after Immersion in Water.

Reducing pollution caused by coal dust has always been a hot issue in the coal mining industry, and the water absorption of coal particles plays an important role in dust reduction. To study the relationship between different immersion time and the water absorption of coal particles, the substance content of coal particles after immersion was analyzed and water absorption characterization of coal particles was carried out by X-ray diffraction (XRD), water absorption calculations, and water absorption measurements. The results indicate that immersion can alter the material content of coal particles, leading to a decrease in the content of soluble mineral kaolinite, thereby affecting the wettability of coal particles.