SSBP1 promotes bovine ephemeral fever virus replication by antagonizing antiviral immune responses via degrading MAVS.

Mitochondrial single-stranded DNA-binding protein 1 (SSBP1), a component of the mitochondrial DNA replisome, is involved in DNA replication, repair and maintenance of mitochondrial DNA. However, its function in the bovine ephemeral fever virus (BEFV) infection has not been described. Herein, we found that SSBP1 acted as an essential negative regulator of mitochondrial antiviral signaling protein (MAVS) to maintain immune homeostasis.

Understanding the potential role of microbial electrolysis cells in promoting electron transfer and microbial metabolism during the drying period in treating metformin-containing wastewater with an adsorption-biological coupling system.

Effective removal of metformin from wastewater through biological treatment technology has been a challenging issue. Enhancing electron transfer was demonstrated to be an effective measure to improve the removal of refractory pollutants from wastewater. In this study, the effects of a microbial electrolysis cell (MEC) in strengthening an adsorption-biological coupling reactor during the drying period in treating metformin wastewater were investigated, along with its microbial community and metabolism.

Pathologic characteristics and management strategies for two categories of prostate cancer patients with low prostate-specific antigen undergoing radical prostatectomy.

Background: Prostate cancer (PCa) with low levels of prostate-specific antigen (PSA) (0-4 ng/mL) includes PCa detected through biopsy and incidental PCa (IPC) in patients with previous prostate surgeries. The study was conducted to compare these two groups of patients undergoing radical prostatectomy (RP), aiming to assess pathological characteristics and suggest strategies for predicting and managing low PSA PCa.

Methods: A retrospective analysis was performed on two categories of low PSA PCa patients.

HBimmCue: A Versatile Fluorescent Probe for Multi-Scale Imaging of Lipid Polarity and Membrane Order in Inner Mitochondrial Membrane.

Mitochondrial membrane environmental dynamics are crucial for understanding function, yet high-resolution observation remains challenging. Here, HBimmCue is introduced as a fluorescent probe localized to inner mitochondrial membrane (IMM) that reports lipid polarity and membrane order changes, which correlate with cellular respiration levels. Using HBimmCue and fluorescence lifetime imaging microscopy (FLIM), IMM lipid heterogeneity is uncovered across scales, from nanoscale structures within individual mitochondria to mouse pre-implantation embryos.

Non vertical ionization-dissociation model for strong IR induced dissociation dynamics of [Formula: see text].

Electron-nuclear coupling plays a crucial role in strong laser induced molecular dissociation dynamics. The interplay between electronic and nuclear degrees of freedom determines the pathways and outcomes of molecular fragmentation. However, a full quantum mechanical treatment of electron-nuclear dynamics is computationally intensive.

Genome-wide association study of sucrose content in vegetable soybean.

Background: Vegetable soybean is an important legume vegetable. High sucrose content is a significant quality characteristic of vegetable soybean that influences consumers' taste. However, the genetic basis of sucrose content in vegetable soybean is currently unclear.

The clinical results and function of the intersegmental disc after posterior-only partial corpectomy for lumbar burst fractures.

We treated the burst spinal fracture with posterior subtotal corpectomy and reconstruction. In some cases, the endplate and the adjacent disc can be preserved during the operation. The adjacent disc retained its mobility after the removal of the posterior pedicle screws.

Metagenomics and metaproteomics reveal the effects of sludge types and inoculation modes on N,N-dimethylformamide degradation pathways and the microbial community involved.

This study demonstrated the effects of the sludge type and inoculation method on the N,N-dimethylformamide degradation pathway and associated microbial communities. The sludge type is critical for DMF metabolism, with acclimatized aerobic sludge having a significant advantage in terms of DMF metabolism performance, whereas acclimatized anaerobic sludge has a reduced DMF metabolism capacity. Metagenomic revealed increased abundances of Methanosarcina, Pelomona and Xanthobacter in the adapted anaerobic sludge, suggesting that anaerobic sludge can utilize the methyl products produced by DMF metabolism for growth.

Effects of flotation reagents with aniline aerofloat and ammonium dibutyl dithiophosphate on a constructed rapid infiltration system: Performance and microbial metabolic pathways.

Aniline aerofloat (AAF) and ammonium dibutyl dithiophosphate (ADD) are the key flotation reagents in mineral processing. This study investigated the performance of the constructed rapid infiltration systems with coke and red mud as adsorbents for treatment AAF and ADD wastewater. Meanwhile, the effects of AAF and ADD on the microbial metabolic pathways of the systems were unraveled.

Soil nutrients and enzyme activities based on millet continuous cropping obstacles.

In order to evaluate the effects of continuous cropping of millet on soil nutrients and soil enzyme activities, the present study was based on four treatments of 2 years of continuous cropping (T1), 3 years of continuous cropping (T2), 4 years of continuous cropping (T3) and rotational cropping (CK), based on 4 years of no fertilizer positioning experiments, and the soil nutrients, soil enzyme activities and millets yields were determined, respectively. The results showed that with the increase of continuous cropping years, the millet yield decreased and was significantly lower than that of rotating with legume crops, and compared with CK, the yields of T1, T2 and T3 treatments were reduced by 8.92%, 13.

Hidden delta degradation due to fluvial sediment decline and intensified marine storms.

Deltas are threatened by erosion due to climate change and reduced sediment supply, but their response to these changes remains poorly quantified. We investigate the abandoned Yellow River delta that has transitioned from rapid growth to ongoing deterioration due to a river avulsion removing the sediment supply. Integrating bathymetric data, process observations, and sediment transport modeling, we find that while the subaerial delta was stabilized by engineering measures, the subaqueous delta continued to erode due to intensified storms, losing 39% of its mass deposited before the avulsion.

Impact analysis of hydraulic residence time and dissolved oxygen on performance efficiency and microbial community in N, N-dimethylformamide wastewater treated by an AnSBR-ASBR.

Suitable operating parameters are one of the key factors to efficient and stable biological wastewater treatment of N, N-dimethylformamide (DMF) wastewater. In this study, an improved AnSBR-ASBR reactor (anaerobic sequencing batch reactor, AnSBR, and aerobic SBR, ASBR, run in series) was used to investigated the effects of operating conditions such as hydraulic residence time (HRT), AnSBR stirring speed and ASBR dissolved oxygen (DO) for DMF wastewater treatment. When HRT decreased from 24 h to 12 h, the average removal rates of COD by the AnSBR were 34.

Use of iron-loaded biochar to alleviate anammox performance inhibition under PFOA stress conditions: Integrated analysis of sludge characteristics and metagenomics.

The negative effects of perfluorooctanoic acid (PFOA) on biological nitrogen removal performance in wastewater treatment plants, are receiving increasing attention due to the widespread reporting of this issue. In this study, pomelo peel iron-loaded biochar (Fe-PBC) was added to an anammox bioreactor to alleviate the negative effects of PFOA. Results showed that the addition of Fe-PBC increased the ammonia and nitrite removal efficiencies from 77.

Local infiltration of HYR-PB21, a sustained-release formulation of bupivacaine, provides analgesia and reduces opioid requirement after haemorrhoidectomy: a randomised controlled trial.

Background: HYR-PB21 is a new sustained-release formulation of bupivacaine indicated for controlling postoperative pain. The objectives of this study were to investigate the analgesic efficacy and safety profile of HYR-PB21 in patients after haemorrhoidectomy.

Methods: This was a multicentre, randomised, double-blind, positive-controlled trial.

Anthocyanin pigmentation as a quantitative visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots.

Visualization of root colonization by arbuscular mycorrhizal fungi (AMF) is the most elementary experiment in the field of mycorrhizal symbiosis. The most widely used approach for evaluating levels of AMF colonization is staining with trypan blue or ink, which is scored using the time-consuming grid intersection method. Here we demonstrate the use of an anthocyanin-based visual marker system for visualizing AMF colonization of Medicago truncatula roots.

Assessment of the potential for quantifying multi-period suspended sediment concentration variations using satellites with different temporal resolution.

Suspended sediment concentration (SSC) is a crucial indicator for coastal health and geomorphological evolution, featured by complex periodic processes on multiple timescales in response to different cyclic forcing factors. Although remote sensing has functioned as an important means for SSC estimation with sufficient spatio-temporal coverage, the low effective sampling rates and resulting unevenly spaced characteristics of the retrieved time series would hamper the extraction of the representative SSC portrayal (amplitude and phase) on multiple timescales, especially for low-resolution satellites. Here, we retrieved a 9-year hourly GOCI SSC time series (January 2012 to December 2020) at two coastal sites in China (Haimen and Haizhou Bay) as reference cases, and utilized them to obtain MODIS, Sentinel and Landsat sequences with average temporal resolutions of 0.

Effects of PFOA on the physicochemical properties of anaerobic granular sludge: Performance evaluation, microbial community and metagenomic analysis.

The impact of perfluorooctanoic acid (PFOA) on the anaerobic granular sludge was evaluated through a sequential batch experiment. Results showed that PFOA inhibited the chemical oxygen demand (COD) removal rate of the sludge and the dosage of 100 mg/L PFOA was more obvious. However, this negative effect would gradually weaken with the adaptation of microorganisms.

Northwestern Pacific tropical cyclone activity enhanced by increased Asian dust emissions during the Little Ice Age.

Instrumental records reveal that intense tropical cyclone (TC) activity varies with tropical sea surface temperature (SST) on annual-decadal scales. Drivers of intense TC activity at the centennial-millennial scale are less clear, due to the sparseness of pre-observational reconstructions. Here, we present a new 2 kyr continuous activity record of intense TCs from offshore eastern China.