Identification and experimental validation of prognostic genes related to cytochrome c in breast cancer.

Breast cancer (BC) is one of the most prevalent malignant diseases affecting women. Cytochrome c (Cyt c) plays a critical role in various pathological processes, however, its precise mechanism in BC remains unclear. This study aimed to identify prognostic genes linked to Cyt c in BC and explore their underlying mechanisms.

The chain mediation effects of general self-efficacy and psychological resilience between physical activity and academic stress among Chinese adolescents.

Introduction: Excessive academic stress is a significant factor influencing the mental health of Chinese adolescents. This study aimed to examine the relationship between physical activity and academic stress and explore the chain-mediated role of general self-efficacy and psychological resilience.

Methods: A survey was administered to 1,230 adolescents using the Physical Activity Questionnaire (PAQ-A), the Academic Stress Scale, the General Self-Efficacy Scale (GSES), and the Psychological Resilience Scale.

Adaptation and psychometric evaluation of the Chinese version of the hip disability and osteoarthritis outcome Score-Physical function Short-form (HOOS-PS).

Background: Chronic hip joint disease has a high prevalence in China, and the primary scale currently used to assess functional recovery after total hip arthroplasty (THA) is the Hip disability and Osteoarthritis Outcome Score - Physical Function Short-form (HOOS-PS). Therefore, our objective was to translate the HOOS-PS scale into Simplified Chinese and evaluate its reliability, validity, and responsiveness in THA patients.

Methods: First, we followed the widely accepted cross-cultural translation process to translate the original HOOS-PS into the Chinese version of HOOS-PS (CHOOS-PS).

Pred5AOP: an efficient screening of food-derived antioxidant peptides based on deep learning, molecular docking, and experimental validation.

Antioxidant peptides derived from dietary proteins positively impact human health due to their high activity and safety. In this study, a database of 76,343 peptides was constructed via in silico hydrolysis of 29 dietary proteins. A novel antioxidant peptide screening method, Pred5AOP, based on the MLP algorithm, identified six representative peptides: DHD, DDHR, TPNT, HYHE, NDHY, and NGDA.

Prediction of postoperative visual cognitive impairment using graph theory and machine learning based on resting-state brain networks.

Background: Visual cognitive impairment is among the most common postoperative cognitive dysfunctions, significantly impacting recovery and quality of life in elderly patients. However, effective preoperative prediction methods remain lacking. We developed a machine learning model using graph theory analysis of resting-state functional connectivity networks to predict postoperative visual cognitive impairment.

Efficacy and safety of robot-assisted versus endo-laparoscopic ventral hernia repair: a meta-analysis of randomized controlled trials.

Purpose: To comprehensively compare the surgical outcomes of robotic ventral hernia repair (rVHR) with traditional endo-laparoscopic ventral hernia repair (lapVHR) using systematic review methods, evaluating the efficacy and safety of rVHR and providing reference for clinical applications of rVHR.

Methods: This systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) list. The following electronic databases were searched: PubMed, Web of science, Cochrane library, Embase, Scopus, and SpringerLink.

A novel colorimetric detection based on bifunctionalized gold nanoparticle combined with machine learning and deep learning models to identify microbial transglutaminase in foods.

Microbial transglutaminase (mTG) is widely used in the food industry to enhance the appearance and texture of meat and fish products, as well as the smoothness and richness of dairy products. However, the undisclosed excessive addition of mTG contributes to various health issues, including celiac disease with intestinal leakage, anemia, osteoporosis, dermatitis, and other parenteral symptoms. In this study, we developed a novel method combining gold nanoparticles (AuNPs), machine learning, and deep learning to study mTG activity in both aqueous solutions and diverse processed foods.

Alterations of Glymphatic System Before and After Shunt Surgery in Patients With Idiopathic Normal Pressure Hydrocephalus: A Longitudinal Study.

Aims: This study aimed to assess the glymphatic dysfunction in idiopathic normal pressure hydrocephalus (iNPH) patients and its recovery post-shunt surgery using diffusion tensor image analysis along perivascular spaces (DTI-ALPS).

Methods: Thirty-five iNPH patients and forty healthy controls (HC) underwent MRI scans and neuropsychological assessments at baseline. A follow-up study, conducted three months post-shunt surgery, included fifteen iNPH patients.

Individual-level cortical morphological network analysis in idiopathic normal pressure hydrocephalus: diagnostic and prognostic insights.

Background: Idiopathic normal-pressure hydrocephalus (iNPH) is a neurodegenerative disorder characterized by treatable cognitive impairment, remains poorly understood in terms of its underlying pathological mechanisms. Cortical morphological similarity network, which quantify synchronized morphological changes across brain regions, offer novel insights into inter-individual neuroanatomical variability. This study investigates individual-level cortical morphological network patterns in iNPH, explores their diagnostic utility and prognostic value for postoperative outcomes.

Differential Expression of Disulfidptosis-Related Genes in Spinal Cord Injury and Their Role in the Immune Microenvironment.

Spinal cord injury (SCI) often results in severe sensory, motor, and autonomic dysfunction, with limited treatment options due to complex underlying mechanisms. Disulfidptosis, a recently discovered form of cell death driven by disulfide bond accumulation, has been linked to various diseases, but its role in SCI remains unexplored. This study investigates the involvement of disulfidptosis-related genes (DRGs) in SCI to identify potential diagnostic markers and therapeutic targets.

Toward a Small-Molecule Antagonist Radioligand for Positron Emission Tomography Imaging of the Mu Opioid Receptor.

The opioid crisis is a catastrophic health emergency catalyzed by the misuse of opioids that target and activate the mu opioid receptor. Many traditional radioligands used to study the mu opioid receptor are often tightly regulated owing to their abuse and respiratory depression potential. Of those that are not regulated, a lack of opioid receptor subtype selectivity can cause confounding in interpreting results.

Bilateral non-traumatic multiple calf muscle hernias in a young adult: a rare case report.

Background: Muscle hernia refers to the localized protrusion of muscle from a weak point due to fascia defects. Muscle hernias can be classified as structural or traumatic. There are many reasons for the formation of fascia defects.

Cognitive outcomes after extracranial-intracranial bypass surgery in elderly patients diagnosed with atherosclerotic cerebral steno-occlusive artery disease.

Background: The safety and clinical effectiveness of extracranial-intracranial (EC-IC) bypass surgery in elderly patients with atherosclerotic internal carotid artery and/or middle cerebral artery steno-occlusive (ACMSO) disease remain ambiguous. Here, we analyzed our experience of EC-IC bypass surgery to evaluate its clinical safety and effect on the cognitive function for elderly patients with ACMSO.

Methods: This retrospective study enrolled patients >60 years of age diagnosed with ACMSO who underwent EC-IC bypass surgery at the authors' center between January 2018 and January 2021.

Characterization of cross-resistance gene optrA-carrying Campylobacter coli.

Campylobacter is a significant foodborne pathogen causing human campylobacteriosis. The optrA gene, which encodes an ABC-F protein, confers cross-resistance to oxazolidinones and phenicols, and recent studies have identified the emergence of optrA in Campylobacter. However, detailed information of optrA- carrying C.

Noninvasive Detection of Oxidative Stress in a Mouse Model of 4R Tauopathy via Positron Emission Tomography with [F]ROStrace.

Oxidative stress, defined as the excessive production of reactive oxygen species (ROS), is a crucial factor in the pathogenesis of various neurodegenerative diseases, including the 4-repeat (4R) tauopathies. Collectively, the 4R tauopathies are characterized by the progressive aggregation of tau protein isoforms with four microtubule-binding domains in and around brain cells. The cyclical relationship between oxidative stress and 4R tau aggregation suggests that a means of imaging ROS noninvasively could be a valuable tool for the study and treatment of 4R tauopathy in both humans and animal models.

Iodinated Copper-Cysteamine Nanoparticles as Radiosensitizers for Tumor Radiotherapy.

Radiotherapy is a widely applied first-line clinical treatment modality of cancer. Copper-cysteamine (Cu-Cy) nanoparticles represent a new type of photosensitizer that demonstrates significant anti-tumor potential by X-ray-induced photodynamic therapy. Iodide is a high-Z element with superior X-ray absorption ability and has the β-decay radiotherapeutic nuclide, I, which emits Cherenkov light.

Prevalence of idiopathic normal pressure hydrocephalus in older adult population in Shanghai, China: A population-based observational study.

Introduction: Idiopathic normal pressure hydrocephalus (iNPH), a condition that primarily affects the elderly, has an unclear prevalence rate in China.

Methods: A cross-sectional survey involving 1491 seniors aged 60 and above in Shanghai was conducted. Clinical symptoms and brain imaging data were collected for the diagnosis of suboptimal probable iNPH (s-probable iNPH).

Graph analysis based on SCN reveals novel neuroanatomical targets related to tinnitus distress.

Purpose: Tinnitus is considered a neurological disorder affecting both auditory and nonauditory networks. This study aimed to investigate the structural brain covariance network in tinnitus patients and analyze its altered topological properties.

Materials: Fifty three primary tinnitus patients and 67 age- and sex-matched healthy controls (HCs) were included.

Conditional Relay Activation of Theranostic Prodrug by Pretargeting Bioorthogonal Trigger and Fluorescence-Guided Visible Light Irradiation.

Bioorthogonalized light-responsive click-and-uncage platform has enabled precise cell surface engineering and timed payload release, but most of such photoactivatable prodrugs have "always-on" photoactivity leading to the dark toxicity. On the other hand, the conditionally activatable photocage is limited to the application of fluorogenic probe/photosensitizer liberation. Herein, we devise a conditionally activatable theranostic platform based on the tetrazine (Tz)-boron-dipyrromethene (BODIPY) construct, in which tetrazine serves as a quencher motif to disable both the fluorescence and photoresponsivity of BODIPY.

Development and validation of a lung biological equivalent dose-based multiregional radiomic model for predicting symptomatic radiation pneumonitis after SBRT in lung cancer patients.

Background: This study aimed to develop and validate a multiregional radiomic-based composite model to predict symptomatic radiation pneumonitis (SRP) in non-small cell lung cancer (NSCLC) patients treated with stereotactic body radiation therapy (SBRT).

Materials And Methods: 189 patients from two institutions were allocated into training, internal validation and external testing cohorts. The associations between the SRP and clinic-dosimetric factors were analyzed using univariate and multivariate regression.

Mapping the evolution of PET/MR research: a bibliometric analysis of publication trends, leading contributors, and conceptual frameworks (2011-2023).

Background: Positron emission tomography-magnetic resonance imaging (PET/MR) is a cutting-edge hybrid imaging technology with the potential to revolutionize medical diagnosis. This bibliometric study aims to map the research landscape of PET/MR by analyzing a curated set of Web of Science Core Collection documents from 2011 to 2023.

Methods: We conducted a bibliometric analysis to map the research landscape of PET/MR, leveraging a curated dataset of 3,600 documents retrieved from the Web of Science Core Collection spanning the period from 2011 to 2023.

Kinetic Analysis and Metabolism of Poly(Adenosine Diphosphate-Ribose) Polymerase-1-Targeted F-Fluorthanatrace PET in Breast Cancer.

The poly(adenosine diphosphate-ribose) polymerase inhibitors (PARPi) have demonstrated efficacy in ovarian, breast, and prostate cancers, but current biomarkers do not consistently predict clinical benefit. F-fluorthanatrace (F-FTT) is an analog to rucaparib, a clinically approved PARPi, and is a candidate biomarker for PARPi response. This study intends to characterize F-FTT pharmacokinetics in breast cancer and optimize image timing for clinical trials.

PET Imaging with [F]ROStrace Detects Oxidative Stress and Predicts Parkinson's Disease Progression in Mice.

Although the precise molecular mechanisms responsible for neuronal death and motor dysfunction in late-onset Parkinson's disease (PD) are unknown, evidence suggests that mitochondrial dysfunction and neuroinflammation occur early, leading to a collective increase in reactive oxygen species (ROS) production and oxidative stress. However, the lack of methods for tracking oxidative stress in the living brain has precluded its use as a potential biomarker. The goal of the current study is to address this need through the evaluation of the first superoxide (O)-sensitive radioactive tracer, [F]ROStrace, in a model of late-onset PD.

A Small-molecule Antagonist Radiotracer for Positron Emission Tomography Imaging of the Mu Opioid Receptor.

The opioid crisis is a catastrophic health emergency catalyzed by the misuse of opioids that target and activate the mu opioid receptor. Traditional radioligands used to study the mu opioid receptor are often tightly regulated owing to their abuse and respiratory depression potential. In the present study, we sought to design and characterize a library of 24 non-agonist ligands for the mu opioid receptor.

A tau dephosphorylation-targeting chimeraselectively recruits protein phosphatase-1 to ameliorate Alzheimer's disease and tauopathies.

Abnormal accumulation of hyperphosphorylated tau (pTau) is a major cause of neurodegeneration in Alzheimer's disease (AD) and related tauopathies. Therefore, reducing pTau holds therapeutic promise for these diseases. Here, we developed a chimeric peptide, named D20, for selective facilitation of tau dephosphorylation by recruiting protein phosphatase 1 (PP1) to tau.