Spatial-temporal lipidomics reveals dysregulated lipid metabolism in mouse brain during Alzheimer's disease progression.

Introduction: Tracking spatial lipidomic changes during Alzheimer's disease (AD) progression is crucial for elucidating the underlying mechanisms of the disease.

Objectives: This study aims to investigate the spatial-temporal lipidomic alterations and their associated metabolic enzyme changes during AD progression.

Methods: The spatial lipidomic changes and corresponding alterations in metabolic enzymes during AD progression in amyloid precursor protein/presenilin-1 (APP/PS1) mice were thoroughly analyzed using an advanced ambient mass spectrometry imaging (MSI) technique, complemented by immunofluorescence (IF) imaging.

Coexistence of human pegivirus infection and poor hematopoietic reconstruction in a lymphoma patient after autologous hematopoietic stem cell transplantation: A case report and literature review.

Rationale: Lymphoblastic lymphoma (LBL) is an exceptionally aggressive form of lymphoma. Following the achievement of complete remission through induction therapy, it is imperative to proceed with consolidation and intensive treatment, subsequently followed by hematopoietic stem cell transplantation (HSCT) at the earliest opportunity. Hepatitis G virus (HPgV), an ribonucleic acid virus transmitted via blood, also known as human pegivirus, exhibits a significantly higher positivity rate among HSCT patients compared to the general population.

Efficient Synthesis of Clovibactin and Its Analogues: Promoting the Discovery of More Potent Antibiotics via Multiple Screenings.

Increasingly, antimicrobial resistance has become a major concern for public health. The recently discovered cyclic depsipeptide, clovibactin, is a potential antibiotic candidate with potent activities against multidrug-resistant pathogens. It contains three d-amino acids, including a rare d-hydroxyasparagine residue.

Incidence, Risk Factors, and Outcomes of Seizures in Extracorporeal Cardiopulmonary Resuscitation Patients: An Analysis of the Extracorporeal Life Support Organization Registry.

Extracorporeal cardiopulmonary resuscitation (ECPR) for refractory cardiac arrest is increasing in usage, but risk factors for seizures are largely unknown. We included adult ECPR patients in the Extracorporeal Life Support Organization Registry from 512 extracorporeal membrane oxygenation (ECMO) centers (2013-2023). Seizures were determined clinically or by electroencephalogram.

Deep-learning-based endoscopic single-shot fringe projection profilometry.

Significance: Conventional fringe projection profilometry (FPP) requires multiple image acquisitions and therefore long acquisition times that make it slow for high-speed dynamic measurements. We propose and demonstrate a deep-learning-based single-shot FPP system utilizing a single endoscope for surgical guidance.

Aim: We aim to achieve real-time depth map generation of target tissues with high accuracy for robotic surgical guidance.

Exploring the Association Between Early PaCO Correction Speed and Cerebrovascular Autoregulation in a Porcine Model of Extracorporeal Resuscitation.

Background: Prior clinical research demonstrated that rapid reduction in arterial carbon dioxide (PaCO) levels during extracorporeal membrane oxygenation (ECMO) is associated with acute brain injury (ABI), which may be due to sudden cerebral vasoconstriction and impaired cerebrovascular autoregulation (CVAR). However, the causal relationship between rapid PaCO correction and its impact on ABI has not been firmly established due to the lack of high-quality evidence. We aimed to investigate whether rapid PaCO correction following extracorporeal cardiopulmonary resuscitation (ECPR) causes CVAR impairment and neuronal injury in a porcine model.

Enhanced Ionic Conductivity in Bidisperse Solid Electrolytes.

The search for safe, reliable, and compact high-capacity energy storage devices has led to an increased interest in all-solid-state battery research. Solid electrolytes provide enhanced safety and durability due to their reduced volatility and increased mechanical strength compared with organic liquid electrolytes. Using a particle dynamics electromechanics computational model, the ionic conductivity of a solid electrolyte comprised of bidisperse grain size consisting of a mixture of coarse and fine particles is examined.

Global, regional, and country-specific lifetime risks of osteoarthritis, 1990-2021: a systematic analysis for the global burden of disease study 2021.

Background: Osteoarthritis (OA) is a leading cause of disability worldwide, yet global estimates of lifetime risk remain limited. This study aims to provide comprehensive global, regional, and national lifetime risk estimates for OA, including knee, hand, and hip subtypes, from 1990 to 2021.

Methods: Using data from the Global Burden of Disease (GBD) Study 2021, we estimated the lifetime risk of developing OA from age 20 onwards, stratified by sex, location, and Socio-demographic Index (SDI).

Bering: joint cell segmentation and annotation for spatial transcriptomics with transferred graph embeddings.

Single-cell spatial transcriptomics can provide subcellular resolution for a deep understanding of molecular mechanisms. However, accurate segmentation and annotation remain a major challenge that limits downstream analysis. Current machine learning methods heavily rely on nuclei or cell body staining, resulting in the significant loss of both transcriptome depth and the limited ability to learn spatial colocalization patterns.

Efficacy of Holmium Laser Enucleation of the Prostate in Patients With Detrusor Underactivity or Acontractile Bladder.

Purpose: This study evaluates the efficacy of holmium laser enucleation of the prostate (HoLEP) as a deobstructive surgical intervention for patients with detrusor underactivity (DU) or an acontractile bladder (AcB).

Methods: Between April 2021 and May 2024, patients undergoing HoLEP for benign prostatic obstruction (BPO) were selected based on urodynamic study results indicating DU or AcB. The primary outcome was the change in voiding efficiency (VE) following HoLEP.

Targeting CD155 in lung adenocarcinoma: A5 nanobody-based therapeutics for precision treatment and enhanced drug delivery.

This study presents a novel approach targeting CD155, an overexpressed protein in lung adenocarcinoma (LUAD), using nanobodies with exceptional precision and efficacy. The significant upregulation of CD155 in LUAD, associated with poor patient outcomes, highlights its potential as a therapeutic target. An anti-CD155 nanobody (A5 Nb) is developed that binds to CD155-positive lung cancer cells with high affinity (A5 Nb K = 0.

Evaluation of radiation-induced bone marrow toxicity using artificial intelligence-based image analysis in mice.

Radiation exposure profoundly affects bone marrow, with even low-dose exposure inducing notable biological changes. Traditional pathology methods often face challenges in detecting subtle alterations. However, artificial intelligence (AI) offers enhanced sensitivity and precision, enabling a more detailed analysis.

Investigating the dynamics of intracranial pressure and cerebral autoregulation during extracorporeal cardiopulmonary resuscitation using a porcine model.

Background: Extracorporeal cardiopulmonary resuscitation (ECPR) has emerged as a promising intervention for refractory cardiac arrest, with potential benefits in survival and neurological outcomes. However, the impact of ECPR's rapid, non-pulsatile restoration of cerebral blood flow on intracranial physiology and autoregulation remains poorly understood.

Methods: Using a porcine model, this study investigated the dynamics of intracranial pressure (ICP), cerebral autoregulation, and pulsatility during key experimental phases: Baseline, Fibrillation, ECMO, and ROSC.

A cross-sectional study on the knowledge, attitudes and practice of chewing areca nut and its risk factors among college students.

Chewing areca nut is common in many nations. However, chewing areca nut may do more harm than good. We aimed to figure out the knowledge, attitudes, and practices of areca nut chewing among college students.

Surgical Outcomes With Neoadjuvant Durvalumab Plus Chemotherapy Followed by Adjuvant Durvalumab in Resectable NSCLC.

Introduction: In AEGEAN, perioperative durvalumab plus neoadjuvant chemotherapy, versus neoadjuvant chemotherapy alone, significantly improved event-free survival (p = 0.004) and pathologic complete response (p < 0.001; primary end points; modified intention-to-treat [mITT] population, which excluded patients with known EGFR or ALK aberrations) with a manageable safety profile in patients with resectable (R)-NSCLC.

Carbon-Coated Silver Nanoparticles for Highly Robust Chloride Capture in Electrochemical Desalination and Lithium Recovery.

In recent years, electrochemical desalination and resource recovery processes have gained significant attention as means of utilizing waters, wherein the majority of anionic species are chloride ions. There have been research efforts to develop high-performance electrode materials for the chloride uptake. Silver with extremely fast kinetics is an ideal option, but the cost and stability issues are yet to be resolved.

Impact of Obesity on Neurologic Outcomes in Patients Undergoing Venoarterial Extracorporeal Membrane Oxygenation.

Objective: To investigate the impact of body mass index (BMI) on neurologic outcomes in venoarterial extracorporeal membrane oxygenation (VA-ECMO) DESIGN: Retrospective observational study SETTING: Single center PARTICIPANTS: Adults INTERVENTIONS: VA-ECMO MEASUREMENTS AND MAIN RESULTS: Obese (BMI≥30 kg/m²) and nonobese (BMI<30 kg/m²) patient groups were compared. The primary outcome was neurologic outcomes at discharge, with "good" (modified Rankin Scale [mRS] score 0-3) and "poor" (mRS score 4-6). Secondary outcomes were acute brain injury (ABI) and mortality.

Carbonation impact on the stabilization of heavy metals in carbon-rich geopolymers based on gasification slag and fly ash.

Carbonization is a common phenomenon that occurs when carbon-rich geopolymers engages in heavy metals' stabilization. The carbonation impact on the stabilization effect remains controversy. This study investigates the carbonation course and the relative migration and transportation of heavy metals in carbon-rich geopolymer with different type of ions.

Associations of tongue and hyoid position, tongue volume, and pharyngeal airway dimensions with various dentoskeletal growth patterns.

Objective: This study investigated the association between tongue and hyoid position, tongue volume, and pharyngeal airway dimensions with craniofacial growth patterns in the sagittal, vertical, and transverse planes.

Methods: Cone beam computed tomography was used to assess 185 non-growing subjects (mean age, 28.7 ± 9.

Fracture structure and dynamic characteristics of coal after various soaking times with supercritical CO.

Current research on the mechanical properties and damage of coal after supercritical CO (sCO) soaking has focused on static investigations. To understand the dynamic characteristics, researchers often analyze the parameters characterizing the fracture and three-dimensional strain fields of coal before and after sCO soaking. We explored the relationship between the time-dependent damage to coal and the deterioration of dynamic parameters.

Using high-frequency oscillation to detect acute brain injury in extracorporeal membrane oxygenation supported patients.

Objective: This study aims to utilize HFO analysis to enhance existing SSEP modality and develop it as a bedside diagnostic tool for acute brain injury (ABI) detection in Extracorporeal Membrane Oxygenation (ECMO) patients.

Significance: Timely diagnosis of ABI in ECMO patients is challenging due to logistical complexities with computed tomography (CT) and magnetic resonance imaging (MRI). Integrating time-frequency analysis into routine SSEP monitoring for early ABI detection can facilitate timely medical decisions.

Structural study on human microbiome-derived polyketide synthases that assemble genotoxic colibactin.

Colibactin, a human microbiome-derived genotoxin, promotes colorectal cancer by damaging the host gut epithelial genomes. While colibactin is synthesized via a hybrid non-ribosomal peptide synthetase (NRPS)-polyketide synthase (PKS) pathway, known as pks or clb, the structural details of its biosynthetic enzymes remain limited, hindering our understanding of its biosynthesis and clinical application. In this study, we report the cryo-EM structures of two colibactin-producing PKS enzymes, ClbC and ClbI, captured in different reaction states using a substrate-mimic crosslinker.

Experimental study on energy evolution and damage characteristics of unloading coal under cyclic loading.

During deep coal mining, coal is often subjected to vertical cyclic loading and lateral unloading. Understanding the failure characteristics of coal under these combined mechanical conditions is essential for safe extraction. This study utilized the MTS816 system to conduct cyclic loading tests on unloaded coal samples under varying confining pressures.

Simultaneous Targeting of Tumor Cells and Tumor-Associated Macrophages To Reprogram Glioblastoma Using Trypsinized Extracellular Vesicles Carrying Tumor Suppressive MicroRNA.

Glioblastoma (GBM) remains difficult to treat due to poor drug delivery across the blood-brain barrier and an immunosuppressive tumor microenvironment (TME). Tumor-suppressive microRNAs (miRNAs) offer a promising strategy to reprogram both tumor cells and the TME, but inefficient delivery systems limit their clinical application. We previously reported that tumor-suppressive miR-138 regresses tumor growth in preclinical GBM models.

KAT5 regulates neurodevelopmental states associated with G0-like populations in glioblastoma.

Quiescence cancer stem-like cells may play key roles in promoting tumor cell heterogeneity and recurrence for many tumors, including glioblastoma (GBM). Here we show that the protein acetyltransferase KAT5 is a key regulator of transcriptional, epigenetic, and proliferative heterogeneity impacting transitions into G0-like states in GBM. KAT5 activity suppresses the emergence of quiescent subpopulations with neurodevelopmental progenitor characteristics, while promoting GBM stem-like cell (GSC) self-renewal through coordinately regulating E2F- and MYC- transcriptional networks with protein translation.